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T225 Flasks for Cell Culture
T225 flask volume and surface area
Start your scale-up with a T225 flask that has the surface and ancillary options you're looking for in a tissue culture flask. Choose a cell culture flask from a collection that includes straight-neck or angled-neck options and surface treatment alternatives that help ensure your healthy tissue cells expand and grow in the flask of your choice.
Some useful numbers such as surface area and volumes of dissociation solutions are given below for T225 flasks.
Please note: The number of cells on a flask will vary with cell type. For this table, 6.3 x 106 HeLa cells were seeded and the confluent flask contained 30 x 106 cells.
| T225 flasks have a surface area of 225 cm2 | ||
|---|---|---|
| Dissociation reagent | Volume of reagent | Volume of growth medium |
| TrypLE Express enzyme | 22.0 mL | 45–68 mL |
| TrypLE Select (1x) enzyme | 22.0 mL | |
| TrypLE Select (10x) enzyme | 2.2 mL diluted to 22.0mL in DPBS/1 mM EDTA | |
| Versene reagent | 22.0 mL | |
| Trypsin | 22.0 mL | |
T225 flask: EasYFlask products
Ergonomically designed for maximal access to the growth surface, this low-profile flask optimizes incubator space and features angled walls for complete viewing under microscopes. Additional features include multiple cap options, and sterilization by gamma irradiation with non-pyrogenic certification.
Thermo Scientific BioLite T225 flask surface
Thermo Scientific BioLite cell culture treated flasks with filter cap and seal cap options suit a multitude of experimental needs while providing cost efficiency for a wide selection of applications in your cell culture routine.
Caps come in both vented and plug seal varieties.
Nunclon Delta treated T225 flasks
Composed of cell culture-treated polystyrene and surface treated with proprietary Nunclon Delta surface, these T225 flasks support optimal growth and attachment of cells to the growth surface.
Filtered caps allow for constant airflow while vent/close caps allow for sufficient gas exchange.
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Are you on the front lines of cancer research?
Surface selection: Achieving optimal cell growth and harvesting
Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.
- Surface modifications to improve cell adhesion
- Characterization of Nunc cell culture surfaces by adhesion properties
- Alternative methods for advanced cell harvesting
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Learn about how to build specific 3D cell spheroid models and platforms to answer the pressing and relevant questions in cancer research.
- Discerning the advantages and disadvantages of 2D versus 3D cell culturing approaches
- Understanding the method of rotating micro-gravity bioreactors as a means to produce long-term spheroid cultures
- Implementing spheroid cultures in cancer research
Watch more cell culture webinars
Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.
Featured article
Cell adhesion and growth on coated or modified glass or plastic surfaces
Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge.