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Extracellular Matrices for PSC Culture
Whether you are culturing your cells on feeder layers or require feeder-free, xeno-free conditions for more clinical applications, Gibco products provide you with all the choices to culture your pluripotent stem cells in the right environment. W e provide extracellular matrices (ECMs) that are designed to:
- Minimize adaptation time,
- Maximize cell performance, and
- Help meet regulatory requirements
Which extracellular matrix is right for you?
| Order now | Order now | Order now | Order now | |
| Most commonly used basement membrane matrix for ESC and iPSC cultures | Simplified solution for the most commonly used basement membrane | Optimized for use with Essential 8 Medium | Superior recovery during challenging transitions | |
|---|---|---|---|---|
| Product name | Geltrex LDEV-Free hESC Qualified | Geltrex hESC Qualified, Ready-To-Use | Vitronectin (VTN-N) | Laminin 521 |
| CTS version available | Not currently available | CTS CELLstart Substrate | CTS Vitronectin (VTN-N) Recombinant Human Protein | Not currently available |
| Recommended applications | Feeder and feeder-free culture | Feeder and feeder-free culture | Feeder-free and xeno-free culture | Feeder-free and xeno-free culture |
| Recommended medium pairing | StemPro hESC SFM or StemFlex Medium | StemPro hESC SFM or StemFlex Medium | StemFlex Medium or Essential 8 Flex Medium | StemFlex Medium or Essential 8 Flex Medium |
| Source origin | Cultured mouse EHS tumor cells; undefined | Cultured mouse EHS tumor cells; undefined | Human (recombinant); defined | Human (recombinant); defined |
| Storage temperature | Frozen (-20 to -80° C) | Refrigerate (2-8° C) | Frozen (-20 to -80° C) | Frozen (-20 to -80° C) |
| Reagent preparation time | Thaw on ice, overnight in refrigerator, dilute 1:100 | Ready to use | Thaw at room temperature, dilute 1:100 | Thaw at 2-8° C, dilute 1:50 |
| Unit size | 1 mL or 5 mL | 50 mL | 1 mL | 1 mL or 10 mL |
| Number of plates coated/unit/span | 16x6 well plate from 1 mL vial diluted 1:100 | 8x6 well plates | 16x6 well plates from 1 mL vial diluted 1:100 | 3 x 6 well plate from 1 mL |
Attachment Factor 0.1% Gelatin, (Cat. No. S006100) may be used for feeder-layer attachment.