Passaging Pluripotent Stem Cells | Thermo Fisher Scientific

Pluripotent stem cell passaging or dissociating can be accomplished using various methods depending on your culture medium and application. Whether you prefer to passage your cell enzymatically or manually, Gibco media has the solution for your PSC subculture.

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Which passaging method is right for you?

	Recommended for clump passaging in feeder and feeder-free cultures	Standard reagent for clump passaging in feeder and feeder-free cultures	Quick and gentle dissociation, recommended for single cell passaging	Recommended for clump passaging with feeder-free Essential 8 Medium	Optimal for clinical research applications that require human origin materials only	Quick and easy manual passaging tool for feeder and feeder-free cultures
	Order	Order	Order	Order	Order	Order
Product name	Dispase	Collagenase IV	Accutase	UltraPure 0.5M EDTA	TrypLE Select CTS	StemPro EZPassage Disposable Stem Cell Passaging Tool
Recommended applications	Passaging in clumps	Passaging in clumps	Dissociation to single cells	Passaging in clumps	Passaging in clumps or single cell passaging	Mechanical passaging in clumps
Source origin	Bacillus polymyxa	Clostridium histolyticum	Marine	Chemical animal-origin free	Recombinant enzyme animal-origin free	Not applicable
Form	Lyophilized powder	Lyophilized powder	Liquid, ready to use	Liquid, dilute 1,000x	Liquid, ready to use	Individually wrapped
Storage temperature	2–8°C	2–8°C	2–8°C	Room temperature	Room temperature	Room temperature
Shelf life of liquid format	2 weeks	2 weeks	2 years	2 years	2 years	Not applicable
Inactivation method	Inhibition by dilution (no inhibitors needed)	Inhibition by dilution (no inhibitors needed)	Inhibition by dilution (no inhibitors needed)	Inhibition by dilution (no inhibitors needed)	Inhibition by dilution (no inhibitors needed)	Not applicable
Unit size	5 g	1 g	100 mL	4 x 100 mL	100 mL	Box of 10 units
Optimized concentration for use	2 mg/mL	1 mg/mL for feeder-dependent, 10 mg/mL for feeder-free	1X Solution	0.5 mM	1X Solution	Not applicable
Number of plates passaged/unit	1,250 x 60-mm plates	500 x 60-mm plates at 1 mg/mL, 50 x 60-mm plates at 10 mg/mL	50 x 60-mm plates	50,000 x 60-mm plates	50 x 60-mm plates	10 plates