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Transmitted Light/Colorimetric Imaging Information
Colorimetric imaging can be a useful tool for studying highly autofluorescent tissue, such as heart, because this autofluorescence can significantly interfere with a fluorescent probe signal. Colorimetric detection is also favored when labeling must be compatible with standard histological stains that provide morphological and contextual information. Here we have a collection of educational resources to help you get the best results.
Transmitted light/colorimetric imaging features
BioProbes article
Click-iT EdU and Click-iT TUNEL colorimetric assays for immunohistochemistry
This article describes the conversion of our popular fluorescence-based click chemistry assays for cell proliferation and apoptosis to colorimetric assays.
Scientific poster
Incorporating histological analysis into existing high-content imaging
To address the need for quantitative colorimetric imaging, we have developed a method for analyzing histological samples on a high-content imaging instrument using a specialized bright-field unit.
Transmitted light/colorimetric imaging learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2016) | Blood cell counting using the Countess II FL Automated Cell Counter | automated cell counter, brightfield microscopy, cell counting, cell counting sample preparation, Countess, EVOS, fluorescent dyes, immunophenotyping, ReadyProbes |
| BioProbes article (2007) | in situ hybridization made easy | Alexa Fluor, brightfield microscopy, fluorescent dyes, fluorescence microscopy/fluorescence imaging, gene expression, in situhybridization, nucleic acid detection, sample stability |
| BioProbes article (2013) | The EVOS FL Auto Imaging System—Automated imaging. Simplified | brightfield microscopy, cell structure-all, EVOS, fluorescence microscopy/fluorescence imaging |
| BioProbes article (2014) | Introducing a three-channel cell counter for your benchtop—The new and improved Countess II FL Automated Cell Counter | cell counting, automated cell counter, brightfield microscopy, Countess, EVOS fluorescence microscopy/fluorescence imaging |
| BioProbes article (2015) | CellInsight CX7 High Content Analysis Platform—Accessible technology for high-content analysis | ArrayScan, brightfield microscopy, cell structure CellInsight, high content analysis |
| BioProbes article (2016) | Click-iT EdU and Click-iT TUNEL Colorimetric Assays for immunohistochemistry—Proliferating and apoptotic cells revealed | apoptosis, brightfield microscopy, cell proliferation, Click-iT, colorimetric,fixed-cell imaging, immunohistochemistry (IHC) |
| Protocol | Colorimetric FFPE—trypsin digestion | colorimetric staining, general ab staining, imaging |
| Protocol | Colorimetric FFPE—Low pH antigen retrieval | colorimetric staining, general ab staining, imaging |
| Protocol | Colorimetric FFPE—High pH antigen retrieval | colorimetric staining, general ab staining, imaging |
| Scientific poster (2008) | Quantum dot (Qdot) labeling of gene expression in fresh frozen brain tissue using high-throughput in situ hybridization | in situ hybridization, brightfield microscopy, confocal microscopy,fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, nanocrystals, neuroscience, Qdot, tissue |
| Scientific poster (2015) | Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue | apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC) |
| Scientific poster (2016) | Correlating internalization and potency to accelerate antibody discovery and development | brightfield microscopy, colorimetric, endocytosis and phagocytosis, EVOS,fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC) |
| Scientific poster (2016) | Incorporating histological analysis into existing high content imaging | antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC) |
| Scientific poster (2016) | Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy | apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC) |
| White paper (2015) | Accuracy and precision with the Countess II FL Automated Cell Counter | automated cell counter, brightfield microscopy, cell counting, Countess |
For Research Use Only. Not for use in diagnostic procedures.