Reverse Transcription

Once RNA is quantified, you are ready for reverse transcription. The ideal reverse transcriptase (RT) will reverse-transcribe even the most difficult RNAs, such as those degraded during the purification process or those in limited quantity. Sensitivity, consistency, thermostability, and speed are key attributes to consider when selecting the best RT for your experiment. This is a critical step in gene expression analysis and Thermo Fisher Scientific can be relied upon to deliver products that meet every research need and to produce accurate gene expression results.

Oligonucleotides

Oligonucleotides (oligos) and probes are used at many points within gene expression workflows. While the technology is well established, degraded, or nonspecific oligos can ruin your results. Choose from a wide variety of pre-designed and custom primers and probes, available with more than 50 modifications including MGB, TAMRA, and QSY.

Oligonucleotides

Artistic rendition of DNA base pairings in an oligo

Probes

Reverse transcriptases

Choose from a variety of reverse transcription enzymes and formats, for superior cDNA synthesis results with any type of template RNA. Enjoy proven results, even with the presence of inhibitors or partially degraded RNA, allowing you to salvage suboptimal samples.

Which SuperScript product is right for you?

	Most effective enzyme for all RNA, including difficult templates	Full optimization with flexible priming options for RT-PCR	Highest performance and convenience for RT-qPCR	Highest performance and convenience for RT-qPCR; with integrated gDNA removal	Fast and simple RT-PCR workflow
Product	SuperScript IV Reverse Transcriptase	SuperScript IV First-Strand Synthesis System	SuperScript IV VILO Master Mix	SuperScript IV VILO Master Mix with ezDNase	SuperScript IV One-Step RT-PCR System
Format	Stand-alone enzyme	cDNA synthesis kit	cDNA synthesis master mix	cDNA synthesis master mix with gDNA removal	One-step RT-PCR kit
Optimal reaction temperature	50–55°C	50–55°C	50°C	50°C	50-55°C
RT reaction time	10 min	10 min	10 min	10 min (additional 2 min gDNA removal)	10 min
Protocol	Download	Download	Download	Download	Download

RNA inhibitors

Small amounts of RNases can sometimes co-purify with isolated RNA and compromise downstream applications. These contaminants can be introduced via tips, tubes, and other reagents. RNase inhibitors are used to inhibit and control contaminants.

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