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High quality, pure, intact RNA is critical to most gene expression experiments. The Thermo Fisher Scientific portfolio of RNA preparation products runs both broad and deep, to provide the right solution every time. Choose from silica-column or magnetic bead kits for total RNA extraction. Find the plastics, magnets, and purification instruments best suited to your preferred method. And be confident in your RNase-free workspace with RNase-free lab essentials for every step.
Eliminating nuclease contamination, particularly RNase contamination, is a constant, tedious challenge. We offer several surface decontamination products that are proven effective at eliminating RNase contamination from lab surfaces.
Stop RNase contamination before your experiments are compromised with our catalog of essential RNA products.
For over two decades, our scientists have developed innovative, robust RNA extraction products that make your job easier and your gene expression analysis more precise.
Total RNA extraction | microRNA & small RNA isolation | mRNA extraction | Sequence-specific RNA extraction | Purified RNA | Viral RNA from liquid and cell-free samples | Automated RNA extraction
Total RNA extraction |
Gold standard for highly pure, intact RNA | High-quality RNA in less than 20 minutes | High-throughput purification of RNA and DNA | Complete, no purification system for qRT-PCR results | |
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Isolation method | High purity organic extraction (requires alcohol precipitation) | Fast, convenient silica column | Scalable, flexible format with magnetic beads | Chemical lysis (time to qRT-PCR results) |
Prep time | ~1 hr | < 20 minutes | ~45 minutes | 10 minutes
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Compatible sample types | Most sample types, particularly more difficult to lyse | Cells and tissues | Tissues, cells, blood, serum/plasma, urine, plants | Cells
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30 min isolation from most samples | Recover all RNAs as well as proteins | HTP isolation of total RNA enriched for small RNAs | Complete kit, sample-to-qPCR from cells | |
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RNA types isolated | Small & large RNA molecules | Small & large RNA molecules | Small & large RNA molecules | Small & large RNA molecules |
Prep time | 20 minutes | 30 minutes | <1 hour | 10 minutes |
Compatible sample types | Most sample types | Most sample types | Serum/Plasma, whole blood, Fresh/frozen tissue, cells, urine | Cells |
Isolation method | Highest purity and convenience; includes both organic extraction and silica column | Highest purity and convenience; includes both organic extraction and silica column | Scalable, flexible format with magnetic beads | Cells-to-CT direct lysis, no purification required |
mRNA extraction |
Rapid mRNA purified from total RNA | No purification needed, mRNA direct from crude samples | mRNA from micro-sized samples best for next-generation sequencing of mRNA | HTP mRNA directly or enrich from total RNA | |
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Prep time | 15 minutes | 15 minutes | 15 minutes | 1.5 hours |
Direct isolation of mRNA from crude samples | No | Yes | Yes | Yes |
mRNA enrichment from total RNA | Yes | No | No | Yes |
4 beads in one kit, identify best for your application | Best solution for viscous samples | High surface area for maximum enrichment | |
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Bead size | 1 µm x 2, 2.8 µm x 2 | 2.8 µm | 1 µm |
Biotinylated Ig (µg/mg beads) | up to 20 | up to 10 | up to 20 |
Binding capacity (free biotin) | 6502,500 pmoles/mg beads | 650–1,350 pmoles/mg beads | 2,500 pmoles/mg beads |
Purified RNA |
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Viral RNA from liquid and cell-free samples |
Product | Format | Most suitable downstream application | ||||
|---|---|---|---|---|---|---|
Real-Time RT-PCR | Microarray analysis | Sequencing | Northern blot | Cloning | ||
Total RNA | ||||||
Chaotropic salt-based lysis, silica filter | ||||||
Chaotropic salt-based lysis, silica filter | ||||||
Chaotropic salt-based lysis, magnetic particles | ||||||
Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | ||||||
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Product | Format | Most suitable downstream application | ||||
Real-Time RT-PCR | Microarray analysis | Sequencing | Northern blot | Cloning | ||
Chaotropic salt-based lysis, silica filter, 96-well processing, automation compatible | ||||||
| MagMAX Total Nucleic Acid Isolation Kit | Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | |||||
Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | ||||||
Chaotropic salt-based lysis, magnetic beads, automation compatible | ||||||
Chaotropic salt-based lysis, magnetic particles, automation compatible | ||||||
Chaotropic salt-based lysis, magnetic particles | ||||||
Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | ||||||
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Product | Format | Most suitable downstream application | ||||
Real-Time RT-PCR | Microarray analysis | Sequencing | Northern blot | Cloning | ||
Chaotropic salt-based lysis, silica filter | ||||||
mirVana miRNA Isolation Kit (without phenol - must be purchased separately) | Chaotropic salt-based lysis, silica filter | |||||
Lysis in TRIzol reagent, one-step reagent | ||||||
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Product | Format | Most suitable downstream application | ||||
Real-Time RT-PCR | Microarray analysis | Sequencing | Northern blot | Cloning | ||
Chaotropic salt-based lysis, magnetic beads | ||||||
Chaotropic salt-based lysis, magnetic beads | ||||||
Automated RNA extraction |
KingFisher instruments automate RNA extraction by moving magnetic beads (not liquids), for clean extractions and consistent, repeatable results. Automation enables increased sample output in shorter time, with minimal hands-on effort.
KingFisher Duo Prime |
KingFisher Flex |
KingFisher Presto |
Complact benchtop | Benchtop | Benchtop, integrates with robotic liquid handler |
Low–Medium | Medium–High | Ultrahigh |
Stabilize and preserve the integrity of your RNA, either at the point of collection or post-collection.
Style Sheet for Global Design System
Style Sheet for Komodo Tabs