RNA Quantification

Nucleic acid quantification determines the concentration and quality of nucleic acids prior to proceeding downstream. If you run a gene expression experiment and do not get results, quantifying the RNA in your sample is a useful troubleshooting experiment.

Fluorescence and spectrophotometry are two commonly used quantification methods. They differ in sensitivity, throughput, sample volume required, and information obtained about the sample. Choosing the right technology for your samples, workflow, and throughput can save significant time and money by helping to prevent downstream experimental failures.

Fluorescence

Fluorescent dyes distinguish between different types of nucleic acids (dsDNA, ssDNA, RNA).  We offer a complete portfolio of fluorometers accommodating individual samples or microplate readers for high throughput operations.

Qubit 4 Fluorometer	Qubit Flex Fluorometer	Fluoroskan Microplate Fluorometer	Varioskan LUX Multimode Microplate Reader
Single sample	Eight samples	6 to 384 well plates	6 to 1,536 well plates

Spectrophotometry

UV-Vis absorbance is measured to distinguish between nucleic acids and proteins and to assess purity of the sample.

NanoDrop One/OneC Microvolume UV-Vis Spectrophotometer	NanoDrop 8000 Spectrophotometer	Multiskan SkyHigh Microplate Spectrophotometer	Varioskan LUX Multimode Microplate Reader
Single sample	Eight samples	6 to 384 well plates, cuvettes, μDrop  Plate	6 to 384 well plates, μDrop and μDrop Duo Plates