Invitrogen

CD8a Monoclonal Antibody (OKT8 (OKT-8)), PE, eBioscience™

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CD8a Antibody (12-0086-42) in Flow

Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, APC (Product # 17-0199-42) and Mouse IgG2a kappa Isotype Control, PE (Product # 12-4724-82) (left) or CD8a Monoclonal Antibody, PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-0086-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.03 µg)/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Human

Published species

Bovine, Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT8 (OKT-8)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10732344

Product Specific Information

Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Product # 14-0088 and clone HIT8a, Product # 14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.

Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT8 (OKT-8) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Microbially-Induced Exosomes from Dendritic Cells Promote Paracrine Immune Senescence: Novel Mechanism of Bone Degenerative Disease in Mice.

Aging and disease

Elsayed R,Elashiry M,Liu Y,Morandini AC,El-Awady A,Elashiry MM,Hamrick M,Cutler CW

12-0086-42 was used in Flow cytometry/Cell sorting to conclude that immune senescence, compounded by advanced age, and accelerated by oral dysbiosis and its induced SASP exosomes, plays a pivotal role in the pathophysiology of experimental periodontitis.

Wed Feb 01 00:00:00 EST 2023

Orthotopic T-cell receptor replacement in primary human T cells using CRISPR-Cas9-mediated homology-directed repair.

STAR protocols

Moosmann C,Müller TR,Busch DH,Schober K

12-0086-42 was used in Flow Cytometry to demonstrate the use of CRISPR-Cas9 for simultaneous editing of T-cell receptor α- and β-chains in primary human T cells.

Fri Mar 18 00:00:00 EDT 2022

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

12-0086-42 was used in Flow cytometry/Cell sorting to explore the expression of iNKT cells in psoriasis patients and the effect of secukinumab on them.

Wed Feb 23 00:00:00 EST 2022

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

12-0086-42 was used in Flow Cytometry to characterize the phenotypic profiles of SARS-CoV-2 antigen-reactive T cells.

Mon Jul 26 00:00:00 EDT 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

12-0086-42 was used in Flow Cytometry to characterize the phenotypic profiles of SARS-CoV-2 antigen-reactive T cells.

Mon Jul 26 00:00:00 EDT 2021

Single-cell transcriptome analysis of the heterogeneous effects of differential expression of tumor PD-L1 on responding TCR-T cells.

Theranostics

Ding R,Liu S,Wang S,Chen H,Wang F,Xu Q,Zhu L,Dong X,Gu Y,Zhang X,Chao CC,Gao Q

12-0086-42 was used in Flow Cytometry to evaluate how tumor expression of PD-L1 affects T cell functionality in anti-cancer TCR-T cell therapy.

Mon Aug 02 00:00:00 EDT 2021

New Bicistronic TALENs Greatly Improve Genome Editing.

Current protocols in stem cell biology

Martín-Fernández JM,Fleischer A,Vallejo-Diez S,Palomino E,Sánchez-Gilabert A,Ruiz R,Bejarano Y,Llinàs P,Gayá A,Bachiller D

12-0086-42 was used in Flow Cytometry /test) to present data regarding the use of a new, more efficient type of transcription activator-like effector nuclease (TALEN) for gene editing.

Sun Mar 01 00:00:00 EST 2020

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5.

BMC biotechnology

Lv Y,Xiao FJ,Wang Y,Zou XH,Wang H,Wang HY,Wang LS,Lu ZZ

12-0086-42 was used in Flow Cytometry to show the HAdV-11p fiber pseudotyped HAdV-5 could effectively transduce human T cells when human EF1a promoter was used to control the expression of transgene, suggesting its possible application in T cell immunocellular therapy.

Wed Apr 24 00:00:00 EDT 2019

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2023

Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.

Scientific reports

Reber AJ,Music N,Kim JH,Gansebom S,Chen J,York I

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Tue Apr 17 00:00:00 EDT 2018

Essential role for CCR6 in certain inflammatory diseases demonstrated using specific antagonist and knockin mice.

JCI insight

Robert R,Ang C,Sun G,Juglair L,Lim EX,Mason LJ,Payne NL,Bernard CC,Mackay CR

12-0086 was used in Flow cytometry/Cell sorting to investigate the role of CCR6 in the recruitment of T cells to inflammatory sites.

Thu Aug 03 00:00:00 EDT 2017

Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity.

Science translational medicine

Dendrou CA,Cortes A,Shipman L,Evans HG,Attfield KE,Jostins L,Barber T,Kaur G,Kuttikkatte SB,Leach OA,Desel C,Faergeman SL,Cheeseman J,Neville MJ,Sawcer S,Compston A,Johnson AR,Everett C,Bell JI,Karpe F,Ultsch M,Eigenbrot C,McVean G,Fugger L

12-0086-42 was used in Flow Cytometry to reveal a protective homozygous effect that defined a signalling optimum between autoimmunity and immunodeficiency and identified TYK2 as a potential drug target for autoimmune disorders.

Wed Nov 02 00:00:00 EDT 2016

PD-L1 expression and prognostic impact in glioblastoma.

Neuro-oncology

Nduom EK,Wei J,Yaghi NK,Huang N,Kong LY,Gabrusiewicz K,Ling X,Zhou S,Ivan C,Chen JQ,Burks JK,Fuller GN,Calin GA,Conrad CA,Creasy C,Ritthipichai K,Radvanyi L,Heimberger AB

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Oncology letters

Liu Z,Han H,He X,Li S,Wu C,Yu C,Wang S

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Increased circulating IL-9-producing CD8⁺ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.

Experimental and therapeutic medicine

Wang W,Cheng ZS,Chen YF,Lin YH

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Large-Scale Purification of r28M: A Bispecific scFv Antibody Targeting Human Melanoma Produced in Transgenic Cattle.

PloS one

Spiesberger K,Paulfranz F,Egger A,Reiser J,Vogl C,Rudolf-Scholik J,Mayrhofer C,Grosse-Hovest L,Brem G

12-0086-42 was used in Flow cytometry/Cell sorting to study how the bispecific scFv antibody r28M produced in transgenic cattle targets human melanoma

Mon Jun 27 00:00:00 EDT 2016

TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.

PloS one

Reichwald K,Jørgensen TZ,Skov S

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Tue Nov 10 00:00:00 EST 2015

Autologous Graft versus Host Disease: An Emerging Complication in Patients with Multiple Myeloma.

Bone marrow research

Batra A,Cottler-Fox M,Harville T,Rhodes-Clark BS,Makhoul I,Nakagawa M

12-0086 was used in Flow cytometry/Cell sorting to compare expression of cell surface molecules on hematopoietic progenitor cell products between healthy individuals and sufferers of autologous graft versus host disease.

Fri May 30 00:00:00 EDT 2014

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Fri Oct 13 00:00:00 EDT 2017

MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.

PloS one

Dössinger G,Bunse M,Bet J,Albrecht J,Paszkiewicz PJ,Weißbrich B,Schiedewitz I,Henkel L,Schiemann M,Neuenhahn M,Uckert W,Busch DH

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Epigenetic biomarkers of T-cells in human glioma.

Epigenetics

Wiencke JK,Accomando WP,Zheng S,Patoka J,Dou X,Phillips JJ,Hsuang G,Christensen BC,Houseman EA,Koestler DC,Bracci P,Wiemels JL,Wrensch M,Nelson HH,Kelsey KT

12-0086 was used in Flow cytometry/Cell sorting to develop a new assay to capture glioma immune biology based on quantitative methylation specific PCR (qMSP) of two T-cell genes.

Sat Dec 01 00:00:00 EST 2012

TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.

Molecular immunology

Arimura Y,Vang T,Tautz L,Williams S,Mustelin T

12-0086 was used in Flow cytometry/Cell sorting to investigate how PTP-PEST is down-regulated following T cell activation, and how that can lead to reduced IL-2 secretion following TCR and CD28 stimulation.

Sun Jun 01 00:00:00 EDT 2008

Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.

Vaccine

Co MD,Orphin L,Cruz J,Pazoles P,Rothman AL,Ennis FA,Terajima M

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Mon Apr 07 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8a Monoclonal Antibody (OKT8 (OKT-8)), PE, eBioscience™

CD8a Antibody (12-0086-42) in Flow

Product Details

12-0086-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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