Invitrogen

CD8a Monoclonal Antibody (OKT8 (OKT-8)), eFluor™ 450, eBioscience™

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CD8a Antibody (48-0086-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4724-82) (blue histogram) or Anti-Human CD8a eFluor® 450 (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD8a Antibody in Immunocytochemistry (ICC/IF)

CD8a Antibody in Immunoprecipitation (IP)

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Product Details

48-0086-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 18 publications 18 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Ferret, Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT8 (OKT-8)

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1907412

Product Specific Information

Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Product # 14-0088 and clone HIT8a, Product # 14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.

Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT8 (OKT-8) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Single-cell profiling of the antigen-specific response to BNT162b2 SARS-CoV-2 RNA vaccine.

Nature communications

Kramer KJ,Wilfong EM,Voss K,Barone SM,Shiakolas AR,Raju N,Roe CE,Suryadevara N,Walker LM,Wall SC,Paulo A,Schaefer S,Dahunsi D,Westlake CS,Crowe JE,Carnahan RH,Rathmell JC,Bonami RH,Georgiev IS,Irish JM

48-0086-42 was used in Flow cytometry/Cell sorting to identify an antigen-specific cellular basis of RNA vaccine-based immunity.

Thu Jun 16 00:00:00 EDT 2022

Recruitment of highly cytotoxic CD8⁺ T cell receptors in mild SARS-CoV-2 infection.

Cell reports

Wagner KI,Mateyka LM,Jarosch S,Grass V,Weber S,Schober K,Hammel M,Burrell T,Kalali B,Poppert H,Beyer H,Schambeck S,Holdenrieder S,Strötges-Achatz A,Haselmann V,Neumaier M,Erber J,Priller A,Yazici S,Roggendorf H,Odendahl M,Tonn T,Dick A,Witter K,Mijočević H,Protzer U,Knolle PA,Pichlmair A,Crowell CS,Gerhard M,D'Ippolito E,Busch DH

48-0086-42 was used in Flow Cytometry to isolate T cell receptor (TCR) repertoires specific for immunodominant SARS-CoV-2 epitopes restricted to common human Leukocyte antigen (HLA) class I molecules in convalescent individuals.

Tue Jan 11 00:00:00 EST 2022

SARS-CoV-2 spike protein harnesses SNX27-mediated endocytic recycling pathway.

MedComm

Zhao L,Zhong K,Zhao J,Yong X,Tong A,Jia D

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Immunocytochemistry

Wed Dec 01 00:00:00 EST 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

48-0086-42 was used in Flow Cytometry to characterize the phenotypic profiles of SARS-CoV-2 antigen-reactive T cells.

Mon Jul 26 00:00:00 EDT 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

48-0086-42 was used in Flow Cytometry to characterize the phenotypic profiles of SARS-CoV-2 antigen-reactive T cells.

Mon Jul 26 00:00:00 EDT 2021

Complement-activated interferon-γ-primed human endothelium transpresents interleukin-15 to CD8+ T cells.

The Journal of clinical investigation

Xie CB,Jiang B,Qin L,Tellides G,Kirkiles-Smith NC,Jane-Wit D,Pober JS

48-0086 was used in Flow cytometry/Cell sorting to report that IFN-γ priming induced nuclear expression of IL-15/IL-15Rα complexes in cultured human ECs and that MAC-induced IL-1β stimulated translocation of IL-15/IL-15Rα complexes to the EC surface in a canonical NF-κB-dependent process in which IL-15/IL-15Rα transpresentation increased activation and maturation of alloreactive CD8+ Tem cells.

Wed Jul 01 00:00:00 EDT 2020

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2023

Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.

Scientific reports

Reber AJ,Music N,Kim JH,Gansebom S,Chen J,York I

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Flow Cytometry

Tue Apr 17 00:00:00 EDT 2018

Decidual T Cells Exhibit a Highly Differentiated Phenotype and Demonstrate Potential Fetal Specificity and a Strong Transcriptional Response to IFN.

Journal of immunology (Baltimore, Md. : 1950)

Powell RM,Lissauer D,Tamblyn J,Beggs A,Cox P,Moss P,Kilby MD

48-0086 was used in Flow cytometry/Cell sorting to investigate the specificity and regulation of CD4+ and CD8+ T cells obtained from human third trimester decidua and demonstrated that decidual CD4+ and CD8+ T cells exhibit a highly differentiated effector memory phenotype in comparison with peripheral blood.

Wed Nov 15 00:00:00 EST 2017

PD-L1 expression and prognostic impact in glioblastoma.

Neuro-oncology

Nduom EK,Wei J,Yaghi NK,Huang N,Kong LY,Gabrusiewicz K,Ling X,Zhou S,Ivan C,Chen JQ,Burks JK,Fuller GN,Calin GA,Conrad CA,Creasy C,Ritthipichai K,Radvanyi L,Heimberger AB

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Oncology letters

Liu Z,Han H,He X,Li S,Wu C,Yu C,Wang S

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Increased circulating IL-9-producing CD8⁺ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.

Experimental and therapeutic medicine

Wang W,Cheng ZS,Chen YF,Lin YH

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Peripheral Leukocyte Migration in Ferrets in Response to Infection with Seasonal Influenza Virus.

PloS one

Music N,Reber AJ,Kim JH,York IA

48-0086 was used in Flow cytometry/Cell sorting to show that lymphocytes differentially home to lymph nodes and tissues based on cell type and proximity to the site of infection.

Wed Jul 12 00:00:00 EDT 2017

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Immunoprecipitation

Fri Jun 05 00:00:00 EDT 2015

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Immunoprecipitation

Fri Jun 05 00:00:00 EDT 2015

TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.

PloS one

Reichwald K,Jørgensen TZ,Skov S

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Flow Cytometry

Tue Nov 10 00:00:00 EST 2015

Influenza vaccination accelerates recovery of ferrets from lymphopenia.

PloS one

Music N,Reber AJ,Lipatov AS,Kamal RP,Blanchfield K,Wilson JR,Donis RO,Katz JM,York IA

48-0086 was used in Flow cytometry/Cell sorting to assess the effectiveness of the influenza vaccine in restoring normal blood cell levels.

Wed Oct 07 00:00:00 EDT 2015

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Flow Cytometry

Fri Oct 13 00:00:00 EDT 2017

MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.

PloS one

Dössinger G,Bunse M,Bet J,Albrecht J,Paszkiewicz PJ,Weißbrich B,Schiedewitz I,Henkel L,Schiemann M,Neuenhahn M,Uckert W,Busch DH

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Vaccination against seasonal influenza A/H3N2 virus reduces the induction of heterosubtypic immunity against influenza A/H5N1 virus infection in ferrets.

Journal of virology

Bodewes R,Kreijtz JH,Geelhoed-Mieras MM,van Amerongen G,Verburgh RJ,van Trierum SE,Kuiken T,Fouchier RA,Osterhaus AD,Rimmelzwaan GF

48-0086 was used in Flow cytometry/Cell sorting to investigate how infection with a recent influenza A/H3N2 virus strain induces robust protection in ferrets against infection with a highly pathogenic avian influenza virus of the H5N1 subtype.

Tue Mar 01 00:00:00 EST 2011

Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.

Vaccine

Co MD,Orphin L,Cruz J,Pazoles P,Rothman AL,Ennis FA,Terajima M

Published figure using CD8a monoclonal antibody (Product # 48-0086-42) in Flow Cytometry

Mon Apr 07 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8a Monoclonal Antibody (OKT8 (OKT-8)), eFluor™ 450, eBioscience™

CD8a Antibody (48-0086-42) in Flow

Product Details

48-0086-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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