Invitrogen

CD8a Monoclonal Antibody (OKT8 (OKT-8)), eBioscience™

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Antibody Testing Data (1)
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CD8a Antibody (14-0086-80) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.125 µg of Anti0Human CD8 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were used for analysis.

CD8a Antibody in Immunocytochemistry (ICC/IF)

CD8a Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

14-0086-80

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 18 publications 18 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Functional Assay (Functional)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

OKT8 (OKT-8)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467092

Product Specific Information

Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Product # 14-0088 and clone HIT8a, Product # 14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.

Applications Reported: The OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT8 (OKT-8) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

Published figure using CD8a monoclonal antibody (Product # 14-0086-80) in Flow Cytometry

Mon Jul 26 00:00:00 EDT 2021

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

Published figure using CD8a monoclonal antibody (Product # 14-0086-80) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

Published figure using CD8a monoclonal antibody (Product # 14-0086-80) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2023

Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.

Scientific reports

Reber AJ,Music N,Kim JH,Gansebom S,Chen J,York I

Published figure using CD8a monoclonal antibody (Product # 14-0086-80) in Flow Cytometry

Tue Apr 17 00:00:00 EDT 2018

PD-L1 expression and prognostic impact in glioblastoma.

Neuro-oncology

Nduom EK,Wei J,Yaghi NK,Huang N,Kong LY,Gabrusiewicz K,Ling X,Zhou S,Ivan C,Chen JQ,Burks JK,Fuller GN,Calin GA,Conrad CA,Creasy C,Ritthipichai K,Radvanyi L,Heimberger AB

Published figure using CD8a monoclonal antibody (Product # 14-0086-80) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Oncology letters

Liu Z,Han H,He X,Li S,Wu C,Yu C,Wang S

Published figure using CD8a monoclonal antibody (Product # 14-0086-80) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Increased circulating IL-9-producing CD8⁺ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.

Experimental and therapeutic medicine

Wang W,Cheng ZS,Chen YF,Lin YH

Published figure using CD8a monoclonal antibody (Product # 14-0086-80) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Distinct Metabolic Requirements of Exhausted and Functional Virus-Specific CD8 T Cells in the Same Host.

Cell reports

Schurich A,Pallett LJ,Jajbhay D,Wijngaarden J,Otano I,Gill US,Hansi N,Kennedy PT,Nastouli E,Gilson R,Frezza C,Henson SM,Maini MK

14-0086 was used in Flow cytometry/Cell sorting to demonstrate that functional and exhausted human-virus-specific CD8 T-cells have distinct metabolic phenotypes, shaping their effector capacity.

Tue Aug 02 00:00:00 EDT 2016

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

14-0086 was used in Immunoprecipitation to study the structure of sorting nexins.

Fri Jun 05 00:00:00 EDT 2015

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

14-0086 was used in Immunoprecipitation to study the structure of sorting nexins.

Fri Jun 05 00:00:00 EDT 2015

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

14-0086 was used in Immunoprecipitation to study the structure of sorting nexins.

Fri Jun 05 00:00:00 EDT 2015

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

14-0086 was used in Immunoprecipitation to study the structure of sorting nexins.

Fri Jun 05 00:00:00 EDT 2015

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

14-0086 was used in Immunoprecipitation to study the structure of sorting nexins.

Fri Jun 05 00:00:00 EDT 2015

Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Hartmann N,Giese NA,Giese T,Poschke I,Offringa R,Werner J,Ryschich E

14-0086 was used in Flow cytometry/Cell sorting to analyse how chemokines and extracellular matrix collagen interact in mediating T-cell infiltration in pancreatic ductal adenocarcinoma.

Tue Jul 01 00:00:00 EDT 2014

TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.

PloS one

Reichwald K,Jørgensen TZ,Skov S

Published figure using CD8a monoclonal antibody (Product # 14-0086-80) in Flow Cytometry

Tue Nov 10 00:00:00 EST 2015

Phase I trial of a multi-epitope-pulsed dendritic cell vaccine for patients with newly diagnosed glioblastoma.

Cancer immunology, immunotherapy : CII

Phuphanich S,Wheeler CJ,Rudnick JD,Mazer M,Wang H,Nuño MA,Richardson JE,Fan X,Ji J,Chu RM,Bender JG,Hawkins ES,Patil CG,Black KL,Yu JS

14-0086 was used in Flow cytometry/Cell sorting to study how expression of four ICT-107 targeted antigens in the pre-vaccine tumors correlated with prolonged overall survival and PFS in ND-GBM patients.

Tue Jan 01 00:00:00 EST 2013

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD8a monoclonal antibody (Product # 14-0086-80) in Flow Cytometry

Fri Oct 13 00:00:00 EDT 2017

MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.

PloS one

Dössinger G,Bunse M,Bet J,Albrecht J,Paszkiewicz PJ,Weißbrich B,Schiedewitz I,Henkel L,Schiemann M,Neuenhahn M,Uckert W,Busch DH

Published figure using CD8a monoclonal antibody (Product # 14-0086-80) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Engagement of T-cell antigen receptor and CD4/CD8 co-receptors induces prolonged STAT activation through autocrine/paracrine stimulation in human primary T cells.

Biochemical and biophysical research communications

Chueh FY,Yu CL

14-0086 was used in Functional assays to determine the extent of STAT protein involvement in T-cell antigen receptor signalling.

Fri Sep 21 00:00:00 EDT 2012

Novel serial positive enrichment technology enables clinical multiparameter cell sorting.

PloS one

Stemberger C,Dreher S,Tschulik C,Piossek C,Bet J,Yamamoto TN,Schiemann M,Neuenhahn M,Martin K,Schlapschy M,Skerra A,Schmidt T,Edinger M,Riddell SR,Germeroth L,Busch DH

14-0086 was used in Flow cytometry/Cell sorting to exhibit novel, low-affinity antibody-derived Fab-fragments for use in clinical cell preparations and research.

Mon Aug 27 00:00:00 EDT 2012

Anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement and improve peptide-MHCI tetramer staining.

Journal of immunology (Baltimore, Md. : 1950)

Clement M,Ladell K,Ekeruche-Makinde J,Miles JJ,Edwards ES,Dolton G,Williams T,Schauenburg AJ,Cole DK,Lauder SN,Gallimore AM,Godkin AJ,Burrows SR,Price DA,Sewell AK,Wooldridge L

14-0086 was used in Functional assays to investigate the role of CD8 in CD8(+) T cell activation, showing that anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement.

Fri Jul 15 00:00:00 EDT 2011

DNA methylation prevents CTCF-mediated silencing of the oncogene BCL6 in B cell lymphomas.

The Journal of experimental medicine

Lai AY,Fatemi M,Dhasarathy A,Malone C,Sobol SE,Geigerman C,Jaye DL,Mav D,Shah R,Li L,Wade PA

14-0086 was used in Functional assays to investigate roles of DNA methylation in cancer, showing that it prevents CTCF-mediated silencing of the oncogene BCL6 in B cell lymphomas.

Mon Aug 30 00:00:00 EDT 2010

The Mi-2/NuRD complex associates with pericentromeric heterochromatin during S phase in rapidly proliferating lymphoid cells.

Chromosoma

Helbling Chadwick L,Chadwick BP,Jaye DL,Wade PA

14-0086 was used in Flow cytometry/Cell sorting to propose that Mi-2/NuRD constitutes an enzymatic component of a pathway for assembly and maturation of chromatin.

Sat Aug 01 00:00:00 EDT 2009

PILAR is a novel modulator of human T-cell expansion.

Blood

Huarte E,Cubillos-Ruiz JR,Nesbeth YC,Scarlett UK,Martinez DG,Engle XA,Rigby WF,Pioli PA,Guyre PM,Conejo-Garcia JR

14-0086 was used in Flow cytometry/Cell sorting to identify a novel human transmembrane molecule expressed on T cells that enhances their expansion through CD161.

Fri Aug 15 00:00:00 EDT 2008

Interaction of human PD-L1 and B7-1.

Molecular immunology

Butte MJ,Peña-Cruz V,Kim MJ,Freeman GJ,Sharpe AH

14-0086 was used in Flow cytometry/Cell sorting to investigate whether human B7-1 and PD-L1 can participate in a similar interaction as murine B7-1 and PD-L1 do, showing that there is interaction of human PD-L1 and B7-1.

Fri Aug 01 00:00:00 EDT 2008

Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.

Vaccine

Co MD,Orphin L,Cruz J,Pazoles P,Rothman AL,Ennis FA,Terajima M

Published figure using CD8a monoclonal antibody (Product # 14-0086-80) in Flow Cytometry

Mon Apr 07 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8a Monoclonal Antibody (OKT8 (OKT-8)), eBioscience™

CD8a Antibody (14-0086-80) in Flow

Product Details

14-0086-80

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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