Invitrogen

CD8a Monoclonal Antibody (OKT8 (OKT-8)), Alexa Fluor™ 700, eBioscience™

FIGURE: 1 / 18

CD8a Antibody (56-0086-42) in Flow

Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC (Product # 11-0199-42) and Mouse IgG2a kappa Isotype Control, Alexa Fluor 700 (Product # 56-4724-80) (left) or CD8 Monoclonal Antibody, Alexa Fluor 700 (right). Cells in the lymphocyte gate were used for analysis.

CD8a Antibody in Immunocytochemistry (ICC/IF)

CD8a Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

56-0086-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 22 publications 22 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT8 (OKT-8)

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670753

Product Specific Information

Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8) and clone HIT8a (Product # 14-0089-82) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.

Applications Reported:This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.

Applications Tested: Has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

SARS-CoV-2 spike protein harnesses SNX27-mediated endocytic recycling pathway.

MedComm

Zhao L,Zhong K,Zhao J,Yong X,Tong A,Jia D

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Immunocytochemistry

Wed Dec 01 00:00:00 EST 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry

Mon Jul 26 00:00:00 EDT 2021

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

HIV Replication in Humanized IL-3/GM-CSF-Transgenic NOG Mice.

Pathogens (Basel, Switzerland)

Perdomo-Celis F,Medina-Moreno S,Davis H,Bryant J,Zapata JC

56-0086-42 was used in Flow Cytometry to investigate the dynamics of HIV infection in humanized NOD/Shi-scid-IL2rγnull (NOG) mice bearing the human genes for interleukin (IL)-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) (NOG-EXL mice).

Tue Mar 12 00:00:00 EDT 2019

RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses.

Cell

Bradley T,Peppa D,Pedroza-Pacheco I,Li D,Cain DW,Henao R,Venkat V,Hora B,Chen Y,Vandergrift NA,Overman RG,Edwards RW,Woods CW,Tomaras GD,Ferrari G,Ginsburg GS,Connors M,Cohen MS,Moody MA,Borrow P,Haynes BF

56-0086-42 was used in Flow Cytometry to identify the upregulation of RAB11FIP5 in HIV-1 infected individuals who produce HIV-1-specific broadly neutralising antibodies.

Thu Oct 04 00:00:00 EDT 2018

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2023

Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.

Scientific reports

Reber AJ,Music N,Kim JH,Gansebom S,Chen J,York I

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry

Tue Apr 17 00:00:00 EDT 2018

The chimeric TAC receptor co-opts the T cell receptor yielding robust anti-tumor activity without toxicity.

Nature communications

Helsen CW,Hammill JA,Lau VWC,Mwawasi KA,Afsahi A,Bezverbnaya K,Newhook L,Hayes DL,Aarts C,Bojovic B,Denisova GF,Kwiecien JM,Brain I,Derocher H,Milne K,Nelson BH,Bramson JL

56-0086 was used in Flow cytometry/Cell sorting to show that T cell antigen coupler-engineered T cells may have a superior therapeutic index relative to chimeric antigen receptor-T cells.

Fri Aug 03 00:00:00 EDT 2018

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

56-0086 was used in Flow cytometry/Cell sorting to suggest that despite the fact that helminth parasites and cancer cell lines are extraordinarily disparate, they share the ability to alter the phenotype of human monocytes.

Sun Apr 01 00:00:00 EDT 2018

Transcriptomic analysis of hepatocellular carcinoma reveals molecular features of disease progression and tumor immune biology.

NPJ precision oncology

Okrah K,Tarighat S,Liu B,Koeppen H,Wagle MC,Cheng G,Sun C,Dey A,Chang MT,Sumiyoshi T,Mounir Z,Cummings C,Hampton G,Amler L,Fridlyand J,Hegde PS,Turley SJ,Lackner MR,Huang SM

56-0086 was used in Flow cytometry/Cell sorting to identify unique molecular signatures pertaining to hepatocellular carcinoma disease progression and tumour immunity.

Wed Oct 04 00:00:00 EDT 2023

High frequency of intestinal T_H17 cells correlates with microbiota alterations and disease activity in multiple sclerosis.

Science advances

Cosorich I,Dalla-Costa G,Sorini C,Ferrarese R,Messina MJ,Dolpady J,Radice E,Mariani A,Testoni PA,Canducci F,Comi G,Martinelli V,Falcone M

56-0086 was used in Flow cytometry/Cell sorting to assess the crucial role of the intestinal environment in promoting Th17 cell expansion in human multiple sclerosis patients.

Sat Jul 01 00:00:00 EDT 2017

PD-L1 expression and prognostic impact in glioblastoma.

Neuro-oncology

Nduom EK,Wei J,Yaghi NK,Huang N,Kong LY,Gabrusiewicz K,Ling X,Zhou S,Ivan C,Chen JQ,Burks JK,Fuller GN,Calin GA,Conrad CA,Creasy C,Ritthipichai K,Radvanyi L,Heimberger AB

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Oncology letters

Liu Z,Han H,He X,Li S,Wu C,Yu C,Wang S

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Increased circulating IL-9-producing CD8⁺ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.

Experimental and therapeutic medicine

Wang W,Cheng ZS,Chen YF,Lin YH

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

A fully human IgG1 anti-PD-L1 MAb in an in vitro assay enhances antigen-specific T-cell responses.

Clinical & translational immunology

Grenga I,Donahue RN,Lepone LM,Richards J,Schlom J

56-0086 was used in Flow cytometry/Cell sorting to explore the effects of Avelumab: a checkpoint-blocking mAB targeting PD-L1, on the immune responses in antigen-specific IVS assays.

Sun May 01 00:00:00 EDT 2016

Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.

Nature communications

Bemark M,Hazanov H,Strömberg A,Komban R,Holmqvist J,Köster S,Mattsson J,Sikora P,Mehr R,Lycke NY

56-0086 was used in Flow cytometry/Cell sorting to study the effects of oral immunisation on inducing gut mucosal memory in B cells.

Tue Sep 06 00:00:00 EDT 2016

Phase I Trial of a Yeast-Based Therapeutic Cancer Vaccine (GI-6301) Targeting the Transcription Factor Brachyury.

Cancer immunology research

Heery CR,Singh BH,Rauckhorst M,Marté JL,Donahue RN,Grenga I,Rodell TC,Dahut W,Arlen PM,Madan RA,Schlom J,Gulley JL

56-0086 was used in Immuno-assay to investigate the effect of GI-6301 on Brachyury in clinical trials.

Sun Nov 01 00:00:00 EDT 2015

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Immunofluorescence

Fri Jun 05 00:00:00 EDT 2015

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Immunofluorescence

Fri Jun 05 00:00:00 EDT 2015

Designed ankyrin repeat proteins are effective targeting elements for chimeric antigen receptors.

Journal for immunotherapy of cancer

Hammill JA,VanSeggelen H,Helsen CW,Denisova GF,Evelegh C,Tantalo DG,Bassett JD,Bramson JL

56-0086 was used in Flow cytometry/Cell sorting to investigate the production of chimeric antigen receptors with novel antigen binding attributes.

Thu Dec 17 00:00:00 EST 2015

TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.

PloS one

Reichwald K,Jørgensen TZ,Skov S

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry

Tue Nov 10 00:00:00 EST 2015

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry

Fri Oct 13 00:00:00 EDT 2017

MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.

PloS one

Dössinger G,Bunse M,Bet J,Albrecht J,Paszkiewicz PJ,Weißbrich B,Schiedewitz I,Henkel L,Schiemann M,Neuenhahn M,Uckert W,Busch DH

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

TH17, TH22 and Treg cells are enriched in the healthy human cecum.

PloS one

Wolff MJ,Leung JM,Davenport M,Poles MA,Cho I,Loke P

56-0086 was used in Flow cytometry/Cell sorting to characterise CD4+ T cell populations in mucosal pinch biopsies collected from a healthy population.

Fri Dec 07 00:00:00 EST 2012

Angiopoietin 2 stimulates TIE2-expressing monocytes to suppress T cell activation and to promote regulatory T cell expansion.

Journal of immunology (Baltimore, Md. : 1950)

Coffelt SB,Chen YY,Muthana M,Welford AF,Tal AO,Scholz A,Plate KH,Reiss Y,Murdoch C,De Palma M,Lewis CE

56-0086 was used in Flow cytometry/Cell sorting to suggest that ANGPT2-stimulated TIE2-expressing monocytes/macrophages represent a novel, potent immunosuppressive force in tumours.

Fri Apr 01 00:00:00 EDT 2011

Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.

Vaccine

Co MD,Orphin L,Cruz J,Pazoles P,Rothman AL,Ennis FA,Terajima M

Published figure using CD8a monoclonal antibody (Product # 56-0086-42) in Flow Cytometry

Mon Apr 07 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD8a Monoclonal Antibody (OKT8 (OKT-8)), Alexa Fluor™ 700, eBioscience™

CD8a Antibody (56-0086-42) in Flow

Product Details

56-0086-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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