Invitrogen

CD8a Monoclonal Antibody (OKT8 (OKT-8)), APC, eBioscience™

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CD8a Antibody (17-0086-42) in Flow

Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, PE (Product # 12-0199-42) and Mouse IgG2a kappa Isotype Control, APC (Product # 17-4724-81) (left) or CD8a Monoclonal Antibody, APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-0086-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 22 publications 22 publications

T-Cell Activation (TCA)

- -

Product Specifications

Species Reactivity

Human

Published species

Ferret, Human, Non-human primate

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT8 (OKT-8)

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10667892

Product Specific Information

Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Product # 14-0088 and clone HIT8a, Product # 14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.

Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT8 (OKT-8) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway.

Journal of experimental & clinical cancer research : CR

Liu Y,Xu Q,Deng F,Zheng Z,Luo J,Wang P,Zhou J,Lu X,Zhang L,Chen Z,Zhang Q,Chen Q,Zuo D

17-0086-42 was used in Flow cytometry/Cell sorting to identify HERC2 functions as a previously unknown modulator of the JAK2/STAT3 pathway, thereby promoting inflammation-induced stemness and immune evasion in HCC.

Wed Feb 01 00:00:00 EST 2023

A Novel Homozygous Stop Mutation in IL23R Causes Mendelian Susceptibility to Mycobacterial Disease.

Journal of clinical immunology

Staels F,Lorenzetti F,De Keukeleere K,Willemsen M,Gerbaux M,Neumann J,Tousseyn T,Pasciuto E,De Munter P,Bossuyt X,Gijsbers R,Liston A,Humblet-Baron S,Schrijvers R

17-0086-42 was used in Flow cytometry/Cell sorting to demonstrate that impaired IL-23 immunity caused by a homozygous R381X mutation in IL23R underlies MSMD, corroborating earlier findings with a homozygous p.C115Y IL23R mutation.

Tue Nov 01 00:00:00 EDT 2022

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

Published figure using CD8a monoclonal antibody (Product # 17-0086-42) in Flow Cytometry

Mon Jul 26 00:00:00 EDT 2021

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

The Journal of clinical investigation

Guedan S,Madar A,Casado-Medrano V,Shaw C,Wing A,Liu F,Young RM,June CH,Posey AD

17-0086 was used in Flow cytometry/Cell sorting to suggest modifications to the costimulatory domains of CAR-T cells can enable longer persistence and thereby improve antitumor response.

Mon Jun 01 00:00:00 EDT 2020

A rational mouse model to detect on-target, off-tumor CAR T cell toxicity.

JCI insight

Castellarin M,Sands C,Da T,Scholler J,Graham K,Buza E,Fraietta JA,Zhao Y,June CH

17-0086 was used in Flow cytometry/Cell sorting to develop a mouse model with stable and tunable human Her2 (hHer2) expression on normal hepatic tissue and compare toxicity between affinity-tuned Her2 chimeric antigen receptor T cells (CARTs).

Thu Jul 23 00:00:00 EDT 2020

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

Published figure using CD8a monoclonal antibody (Product # 17-0086-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

Published figure using CD8a monoclonal antibody (Product # 17-0086-42) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2023

Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.

Scientific reports

Reber AJ,Music N,Kim JH,Gansebom S,Chen J,York I

Published figure using CD8a monoclonal antibody (Product # 17-0086-42) in Flow Cytometry

Tue Apr 17 00:00:00 EDT 2018

Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis.

Frontiers in immunology

Lopes DM,Oliveira SC,Page B,Carvalho LP,Carvalho EM,Cardoso LS

17-0086 was used in Flow cytometry/Cell sorting to evaluate the potential of the S.mansoni Sm29 antigen to change aspects of profiles of monocyte derived dendritic cells and lymphocytes from subjects with cutaneous leishmaniasis.

Tue Oct 15 00:00:00 EDT 2019

Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing.

Cell

Zheng C,Zheng L,Yoo JK,Guo H,Zhang Y,Guo X,Kang B,Hu R,Huang JY,Zhang Q,Liu Z,Dong M,Hu X,Ouyang W,Peng J,Zhang Z

17-0086 was used in Flow cytometry/Cell sorting to characterise the RNA expression of infiltrating T cells in liver cancer.

Thu Jun 15 00:00:00 EDT 2017

PD-L1 expression and prognostic impact in glioblastoma.

Neuro-oncology

Nduom EK,Wei J,Yaghi NK,Huang N,Kong LY,Gabrusiewicz K,Ling X,Zhou S,Ivan C,Chen JQ,Burks JK,Fuller GN,Calin GA,Conrad CA,Creasy C,Ritthipichai K,Radvanyi L,Heimberger AB

Published figure using CD8a monoclonal antibody (Product # 17-0086-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Oncology letters

Liu Z,Han H,He X,Li S,Wu C,Yu C,Wang S

17-0086 was used in Flow cytometry/Cell sorting to demonstrate that the galectin-9-Tim-3 pathway may be critical in the immunoevasion of glioma.

Tue Mar 01 00:00:00 EST 2016

Increased circulating IL-9-producing CD8⁺ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.

Experimental and therapeutic medicine

Wang W,Cheng ZS,Chen YF,Lin YH

Published figure using CD8a monoclonal antibody (Product # 17-0086-42) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

PD-1(+) CD8(+) T cells are exhausted in tumours and functional in draining lymph nodes of colorectal cancer patients.

British journal of cancer

Wu X,Zhang H,Xing Q,Cui J,Li J,Li Y,Tan Y,Wang S

17-0086 was used in Flow cytometry/Cell sorting to evaluate the role of PD-1-PD-L1 pathway in CD8(+) T-cell functions in tumour-draining lymph nodes (TDLNs) and tumours of CRC patients.

Tue Sep 23 00:00:00 EDT 2014

NOD-Rag2null IL-2Rγnull mice: an alternative to NOG mice for generation of humanized mice.

Experimental animals

Katano I,Ito R,Kamisako T,Eto T,Ogura T,Kawai K,Suemizu H,Takahashi T,Kawakami Y,Ito M

17-0086 was used in Flow cytometry/Cell sorting to evaluate the usefulness of NR2G mice as humanised mice by comparing human cell engraftment and differentiation among NOG, NR2G, and NOD-scid mice after HSC transplantation.

Mon Feb 09 00:00:00 EST 2015

TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.

PloS one

Reichwald K,Jørgensen TZ,Skov S

Published figure using CD8a monoclonal antibody (Product # 17-0086-42) in Flow Cytometry

Tue Nov 10 00:00:00 EST 2015

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD8a monoclonal antibody (Product # 17-0086-42) in Flow Cytometry

Fri Oct 13 00:00:00 EDT 2017

MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.

PloS one

Dössinger G,Bunse M,Bet J,Albrecht J,Paszkiewicz PJ,Weißbrich B,Schiedewitz I,Henkel L,Schiemann M,Neuenhahn M,Uckert W,Busch DH

Published figure using CD8a monoclonal antibody (Product # 17-0086-42) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement and improve peptide-MHCI tetramer staining.

Journal of immunology (Baltimore, Md. : 1950)

Clement M,Ladell K,Ekeruche-Makinde J,Miles JJ,Edwards ES,Dolton G,Williams T,Schauenburg AJ,Cole DK,Lauder SN,Gallimore AM,Godkin AJ,Burrows SR,Price DA,Sewell AK,Wooldridge L

17-0086-42 was used in Flow Cytometry to investigate the role of CD8 in CD8(+) T cell activation, showing that anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement.

Fri Jul 15 00:00:00 EDT 2011

Serum response factor regulates immediate early host gene expression in Toxoplasma gondii-infected host cells.

PloS one

Wiley M,Teygong C,Phelps E,Radke J,Blader IJ

17-0086 was used in Flow cytometry/Cell sorting to investigate the effect of Toxoplasma infection on Serum Response Factor activation.

Tue Mar 29 00:00:00 EDT 2011

Contemporary seasonal influenza A (H1N1) virus infection primes for a more robust response to split inactivated pandemic influenza A (H1N1) Virus vaccination in ferrets.

Clinical and vaccine immunology : CVI

Ellebedy AH,Fabrizio TP,Kayali G,Oguin TH,Brown SA,Rehg J,Thomas PG,Webby RJ

17-0086 was used in Flow cytometry/Cell sorting to investigate the effect of previous exposure to a contemporary seasonal H1N1 influenza virus followed by subsequent dose of nonadjuvanted monovalent inactivated vaccine on immune response.

Wed Dec 01 00:00:00 EST 2010

Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.

Vaccine

Co MD,Orphin L,Cruz J,Pazoles P,Rothman AL,Ennis FA,Terajima M

Mon Apr 07 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8a Monoclonal Antibody (OKT8 (OKT-8)), APC, eBioscience™

CD8a Antibody (17-0086-42) in Flow

Product Details

17-0086-42

Flow Cytometry (Flow)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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