Invitrogen

CD45R (B220) Monoclonal Antibody (RA3-6B2), Functional Grade, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

93 Published Figures

124 References

View all (99) B220 (CD45R) antibodies

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Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

CD45R (B220) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 95

CD45R (B220) Antibody (16-0452-82) in Flow

Staining of mouse splenocytes with Anti-Human/Mouse CD45R (B220) FITC (left) or APC (right).Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

CD45R (B220) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD45R (B220) Antibody in Immunocytochemistry (ICC/IF)

CD45R (B220) Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

CD45R (B220) Antibody in Immunohistochemistry (IHC)

CD45R (B220) Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

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Product Details

16-0452-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 36 publications 36 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Flow Cytometry (Flow)

0.5 µg/test

View 75 publications 75 publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RA3-6B2

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468959

Product Specific Information

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Applications Reported: The RA3-6B2 antibody has been reported for use in flow cytometric analysis. RA3-6B2 has also been reported in functional studies of B cells.

Applications Tested: The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.

JCI insight

Zhong L,Yao L,Holdreith N,Yu W,Gui T,Miao Z,Elkaim Y,Li M,Gong Y,Pacifici M,Maity A,Busch TM,Joeng KS,Cengel K,Seale P,Tong W,Qin L

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Apr 08 00:00:00 EDT 2022

Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma.

Cell death & disease

Sun Q,Wang Y,Ji H,Sun X,Xie S,Chen L,Li S,Zeng W,Chen R,Tang Q,Zuo J,Hou L,Hosaka K,Lu Y,Liu Y,Ye Y,Yang Y

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Aug 19 00:00:00 EDT 2022

Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis.

Science advances

Ma L,Tang Q,Gao X,Lee J,Lei R,Suzuki M,Zheng D,Ito K,Frenette PS,Dawlaty MM

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Sphk2 deletion is involved in structural abnormalities and Th17 response but does not aggravate colon inflammation induced by sub-chronic stress.

Scientific reports

Martín-Hernández D,Gutiérrez IL,González-Prieto M,MacDowell KS,Robledo-Montaña J,Tendilla-Beltrán H,Calleja-Rodríguez N,Bris ÁG,Ulecia-Morón C,Moreno B,Caso JR,García-Bueno B,Rodrigues-Mascarenhas S,Marín-Jiménez I,Leza JC,Menchén L

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Monocyte-derived dendritic cells link localized secretory IgA deficiency to adaptive immune activation in COPD.

Mucosal immunology

Richmond BW,Mansouri S,Serezani A,Novitskiy S,Blackburn JB,Du RH,Fuseini H,Gutor S,Han W,Schaff J,Vasiukov G,Xin MK,Newcomb DC,Jin L,Blackwell TS,Polosukhin VV

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon Mar 01 00:00:00 EST 2021

A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system.

Computational and structural biotechnology journal

Shen Y,Jiang L,Iyer VS,Raposo B,Dubnovitsky A,Boddul SV,Kasza Z,Wermeling F

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Wed Nov 10 00:00:00 EST 2021

Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation.

Disease models & mechanisms

Dobosz E,Lorenz G,Ribeiro A,Würf V,Wadowska M,Kotlinowski J,Schmaderer C,Potempa J,Fu M,Koziel J,Lech M

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Thu Mar 18 00:00:00 EDT 2021

Defining the Mechanistic Correlates of Protection Conferred by Whole-Cell Vaccination against Pseudomonas aeruginosa Acute Murine Pneumonia.

Infection and immunity

Sen-Kilic E,Blackwood CB,Huckaby AB,Horspool AM,Weaver KL,Malkowski AC,Witt WT,Bevere JR,Damron FH,Barbier M

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2021

Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo.

Stem cell research

Sudo K,Yamazaki S,Wilkinson AC,Nakauchi H,Nakamura Y

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Role of CXCL5 in Regulating Chemotaxis of Innate and Adaptive Leukocytes in Infected Lungs Upon Pulmonary Influenza Infection.

Frontiers in immunology

Guo L,Li N,Yang Z,Li H,Zheng H,Yang J,Chen Y,Zhao X,Mei J,Shi H,Worthen GS,Liu L

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Tue Feb 08 00:00:00 EST 2022

Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia.

Cell death & disease

Zaccagnini G,Greco S,Longo M,Maimone B,Voellenkle C,Fuschi P,Carrara M,Creo P,Maselli D,Tirone M,Mazzone M,Gaetano C,Spinetti G,Martelli F

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2021

Ibrutinib does not prevent kidney fibrosis following acute and chronic injury.

Scientific reports

Belliere J,Casemayou A,Colliou E,El Hachem H,Kounde C,Piedrafita A,Feuillet G,Schanstra JP,Faguer S

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon Jun 07 00:00:00 EDT 2021

High-throughput analysis of lung immune cells in a combined murine model of agriculture dust-triggered airway inflammation with rheumatoid arthritis.

PloS one

Gaurav R,Mikuls TR,Thiele GM,Nelson AJ,Niu M,Guda C,Eudy JD,Barry AE,Wyatt TA,Romberger DJ,Duryee MJ,England BR,Poole JA

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Thu Jul 08 00:00:00 EDT 2021

Proteostasis in dendritic cells is controlled by the PERK signaling axis independently of ATF4.

Life science alliance

Mendes A,Gigan JP,Rodriguez Rodrigues C,Choteau SA,Sanseau D,Barros D,Almeida C,Camosseto V,Chasson L,Paton AW,Paton JC,Argüello RJ,Lennon-Duménil AM,Gatti E,Pierre P

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon Feb 01 00:00:00 EST 2021

Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.

eLife

Hill DL,Whyte CE,Innocentin S,Lee JL,Dooley J,Wang J,James EA,Lee JC,Kwok WW,Zand MS,Liston A,Carr EJ,Linterman MA

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

Metabolic preconditioning in CD4+ T cells restores inducible immune tolerance in lupus-prone mice.

JCI insight

Wilson CS,Stocks BT,Hoopes EM,Rhoads JP,McNew KL,Major AS,Moore DJ

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Oct 08 00:00:00 EDT 2021

The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation.

Nature communications

Shang M,Yang H,Yang R,Chen T,Fu Y,Li Y,Fang X,Zhang K,Zhang J,Li H,Cao X,Gu J,Xiao J,Zhang Q,Liu X,Yu Q,Wang T

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Mon Mar 29 00:00:00 EDT 2021

Type 2 diabetic mice enter a state of spontaneous hibernation-like suspended animation following accumulation of uric acid.

The Journal of biological chemistry

Zhao Y,Cheng R,Zhao Y,Ge W,Yang Y,Ding Z,Xu X,Wang Z,Wu Z,Zhang J

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Targeting Ovarian Carcinoma with TSP-1:CD47 Antagonist TAX2 Activates Anti-Tumor Immunity.

Cancers

Jeanne A,Sarazin T,Charlé M,Moali C,Fichel C,Boulagnon-Rombi C,Callewaert M,Andry MC,Diesis E,Delolme F,Rioult D,Dedieu S

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Thu Oct 07 00:00:00 EDT 2021

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

Wilfahrt D,Philips RL,Lama J,Kizerwetter M,Shapiro MJ,McCue SA,Kennedy MM,Rajcula MJ,Zeng H,Shapiro VS

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Protein Assays and Analysis

Thu Dec 02 00:00:00 EST 2021

Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants.

Science advances

Zhang YN,Paynter J,Sou C,Fourfouris T,Wang Y,Abraham C,Ngo T,Zhang Y,He L,Zhu J

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry (Frozen)

Fri Oct 22 00:00:00 EDT 2021

SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation.

Immunity

Lederer K,Castaño D,Gómez Atria D,Oguin TH,Wang S,Manzoni TB,Muramatsu H,Hogan MJ,Amanat F,Cherubin P,Lundgreen KA,Tam YK,Fan SHY,Eisenlohr LC,Maillard I,Weissman D,Bates P,Krammer F,Sempowski GD,Pardi N,Locci M

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Synergistic factors control kinase-phosphatase organization in B-cells engaged with supported bilayers.

Molecular biology of the cell

Núñez MF,Wisser K,Veatch SL

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunocytochemistry

Thu Mar 19 00:00:00 EDT 2020

Pituitary Adenylate Cyclase-Activating Polypeptide Alleviates Intestinal, Extra-Intestinal and Systemic Inflammatory Responses during Acute Campylobacter jejuni-induced Enterocolitis in Mice.

Pathogens (Basel, Switzerland)

Heimesaat MM,Mousavi S,Kløve S,Genger C,Weschka D,Tamas A,Reglodi D,Bereswill S

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry (Paraffin)

Wed Sep 30 00:00:00 EDT 2020

Pituitary Adenylate Cyclase-Activating Polypeptide Alleviates Intestinal, Extra-Intestinal and Systemic Inflammatory Responses during Acute Campylobacter jejuni-induced Enterocolitis in Mice.

Pathogens (Basel, Switzerland)

Heimesaat MM,Mousavi S,Kløve S,Genger C,Weschka D,Tamas A,Reglodi D,Bereswill S

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry (Paraffin)

Wed Sep 30 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota.

Pathogens (Basel, Switzerland)

Kløve S,Genger C,Mousavi S,Weschka D,Bereswill S,Heimesaat MM

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon May 18 00:00:00 EDT 2020

Ischemia Reperfusion Injury Triggers CXCL13 Release and B-Cell Recruitment After Allogenic Kidney Transplantation.

Frontiers in immunology

Kreimann K,Jang MS,Rong S,Greite R,von Vietinghoff S,Schmitt R,Bräsen JH,Schiffer L,Gerstenberg J,Vijayan V,Dittrich-Breiholz O,Wang L,Karsten CM,Gwinner W,Haller H,Immenschuh S,Gueler F

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon Apr 12 00:00:00 EDT 2021

Overcoming Immunological Challenges to Helper-Dependent Adenoviral Vector-Mediated Long-Term CFTR Expression in Mouse Airways.

Genes

Cao H,Duan R,Hu J

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon May 18 00:00:00 EDT 2020

Myeloid Cell CK2 Regulates Inflammation and Resistance to Bacterial Infection.

Frontiers in immunology

Larson SR,Bortell N,Illies A,Crisler WJ,Matsuda JL,Lenz LL

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity.

Frontiers in immunology

Myers DR,Abram CL,Wildes D,Belwafa A,Welsh AMN,Schulze CJ,Choy TJ,Nguyen T,Omaque N,Hu Y,Singh M,Hansen R,Goldsmith MA,Quintana E,Smith JAM,Lowell CA

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Mon Jun 14 00:00:00 EDT 2021

Fucoxanthin Ameliorates Atopic Dermatitis Symptoms by Regulating Keratinocytes and Regulatory Innate Lymphoid Cells.

International journal of molecular sciences

Natsume C,Aoki N,Aoyama T,Senda K,Matsui M,Ikegami A,Tanaka K,Azuma YT,Fujita T

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Sun Mar 22 00:00:00 EDT 2020

The Host-Specific Intestinal Microbiota Composition Impacts Campylobacter coli Infection in a Clinical Mouse Model of Campylobacteriosis.

Pathogens (Basel, Switzerland)

Heimesaat MM,Genger C,Klove S,Weschka D,Mousavi S,Bereswill S

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Tue Sep 29 00:00:00 EDT 2020

Cep55 overexpression promotes genomic instability and tumorigenesis in mice.

Communications biology

Sinha D,Nag P,Nanayakkara D,Duijf PHG,Burgess A,Raninga P,Smits VAJ,Bain AL,Subramanian G,Wall M,Finnie JW,Kalimutho M,Khanna KK

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Wed Oct 21 00:00:00 EDT 2020

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model.

International journal of molecular sciences

Aung KT,Akiyama K,Kunitomo M,Mun AY,Tosa I,Nguyen HTT,Zhang J,Kohno T,Ono M,Hara ES,Kuboki T

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunocytochemistry

Fri Oct 30 00:00:00 EDT 2020

Requirements for the differentiation of innate T-bet^high memory-phenotype CD4⁺ T lymphocytes under steady state.

Nature communications

Kawabe T,Yi J,Kawajiri A,Hilligan K,Fang D,Ishii N,Yamane H,Zhu J,Jankovic D,Kim KS,Trinchieri G,Sher A

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon Jul 06 00:00:00 EDT 2020

Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis.

Immunity

Castro-Dopico T,Dennison TW,Ferdinand JR,Mathews RJ,Fleming A,Clift D,Stewart BJ,Jing C,Strongili K,Labzin LI,Monk EJM,Saeb-Parsy K,Bryant CE,Clare S,Parkes M,Clatworthy MR

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Tue Apr 16 00:00:00 EDT 2019

Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis.

Immunity

Castro-Dopico T,Dennison TW,Ferdinand JR,Mathews RJ,Fleming A,Clift D,Stewart BJ,Jing C,Strongili K,Labzin LI,Monk EJM,Saeb-Parsy K,Bryant CE,Clare S,Parkes M,Clatworthy MR

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Tue Apr 16 00:00:00 EDT 2019

Distinct Requirements of CHD4 during B Cell Development and Antibody Response.

Cell reports

Yen WF,Sharma R,Cols M,Lau CM,Chaudhry A,Chowdhury P,Yewdell WT,Vaidyanathan B,Sun A,Coffre M,Pucella JN,Chen CC,Jasin M,Sun JC,Rudensky AY,Koralov SB,Chaudhuri J

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Apr 30 00:00:00 EDT 2019

Comprehensive Kinetic Survey of Intestinal, Extra-Intestinal and Systemic Sequelae of Murine Ileitis Following Peroral Low-Dose Toxoplasma gondii Infection.

Frontiers in cellular and infection microbiology

Heimesaat MM,Dunay IR,Bereswill S

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon Dec 09 00:00:00 EST 2019

Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells.

Frontiers in immunology

Xu J,Pei S,Wang Y,Liu J,Qian Y,Huang M,Zhang Y,Xiao Y

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Wed Oct 14 00:00:00 EDT 2020

Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.

Nature communications

Ramakrishna C,Kujawski M,Chu H,Li L,Mazmanian SK,Cantin EM

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue May 14 00:00:00 EDT 2019

The Hematopoietic Oxidase NOX2 Regulates Self-Renewal of Leukemic Stem Cells.

Cell reports

Adane B,Ye H,Khan N,Pei S,Minhajuddin M,Stevens BM,Jones CL,D'Alessandro A,Reisz JA,Zaberezhnyy V,Gasparetto M,Ho TC,Kelly KK,Myers JR,Ashton JM,Siegenthaler J,Kume T,Campbell EL,Pollyea DA,Becker MW,Jordan CT

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Apr 02 00:00:00 EDT 2019

Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.

Nature medicine

Maryanovich M,Zahalka AH,Pierce H,Pinho S,Nakahara F,Asada N,Wei Q,Wang X,Ciero P,Xu J,Leftin A,Frenette PS

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation.

Immunity

Emgård J,Kammoun H,García-Cassani B,Chesné J,Parigi SM,Jacob JM,Cheng HW,Evren E,Das S,Czarnewski P,Sleiers N,Melo-Gonzalez F,Kvedaraite E,Svensson M,Scandella E,Hepworth MR,Huber S,Ludewig B,Peduto L,Villablanca EJ,Veiga-Fernandes H,Pereira JP,Flavell RA,Willinger T

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Tue Jan 16 00:00:00 EST 2018

Microbial symbionts regulate the primary Ig repertoire.

The Journal of experimental medicine

Chen Y,Chaudhary N,Yang N,Granato A,Turner JA,Howard SL,Devereaux C,Zuo T,Shrestha A,Goel RR,Neuberg D,Wesemann DR

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Mon May 07 00:00:00 EDT 2018

MK2 contributes to tumor progression by promoting M2 macrophage polarization and tumor angiogenesis.

Proceedings of the National Academy of Sciences of the United States of America

Suarez-Lopez L,Sriram G,Kong YW,Morandell S,Merrick KA,Hernandez Y,Haigis KM,Yaffe MB

16-0452 was used in Immunohistochemistry to show that p38/MAPKAP Kinase 2 activity in the myeloid compartment promotes tumour progression by supporting tumour neoangiogenesis in vivo.

Tue May 01 00:00:00 EDT 2018

Proapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner.

Frontiers in immunology

Liu R,King A,Bouillet P,Tarlinton DM,Strasser A,Heierhorst J

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Host Tumor Suppressor p18^INK4c Functions as a Potent Cell-Intrinsic Inhibitor of Murine Gammaherpesvirus 68 Reactivation and Pathogenesis.

Journal of virology

Niemeyer BF,Oko LM,Medina EM,Oldenburg DG,White DW,Cool CD,Clambey ET,van Dyk LF

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu Mar 15 00:00:00 EDT 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43.

Experimental and therapeutic medicine

Li Z,Zhang S,Cao L,Li W,Ye YC,Shi ZX,Wang ZR,Sun LX,Wang JW,Jia LT,Wang W

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

Tumor-immune profiling of murine syngeneic tumor models as a framework to guide mechanistic studies and predict therapy response in distinct tumor microenvironments.

PloS one

Yu JW,Bhattacharya S,Yanamandra N,Kilian D,Shi H,Yadavilli S,Katlinskaya Y,Kaczynski H,Conner M,Benson W,Hahn A,Seestaller-Wehr L,Bi M,Vitali NJ,Tsvetkov L,Halsey W,Hughes A,Traini C,Zhou H,Jing J,Lee T,Figueroa DJ,Brett S,Hopson CB,Smothers JF,Hoos A,Srinivasan R

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon Apr 08 00:00:00 EDT 2019

Anti-inflammatory Effects of the Octapeptide NAP in Human Microbiota-Associated Mice Suffering from Subacute Ileitis.

European journal of microbiology & immunology

Escher U,Giladi E,Dunay IR,Bereswill S,Gozes I,Heimesaat MM

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry (Paraffin)

Mon Jun 25 00:00:00 EDT 2018

Anti-inflammatory Effects of the Octapeptide NAP in Human Microbiota-Associated Mice Suffering from Subacute Ileitis.

European journal of microbiology & immunology

Escher U,Giladi E,Dunay IR,Bereswill S,Gozes I,Heimesaat MM

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry (Paraffin)

Mon Jun 25 00:00:00 EDT 2018

Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice.

Frontiers in immunology

Perez-Chacon G,Adrados M,Vallejo-Cremades MT,Lefebvre S,Reed JC,Zapata JM

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Oct 25 00:00:00 EDT 2019

ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.

Frontiers in immunology

Glal D,Sudhakar JN,Lu HH,Liu MC,Chiang HY,Liu YC,Cheng CF,Shui JW

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu Sep 26 00:00:00 EDT 2019

Multidrug-resistant Pseudomonas aeruginosa aggravates inflammatory responses in murine chronic colitis.

Scientific reports

von Klitzing E,Ekmekciu I,Kühl AA,Bereswill S,Heimesaat MM

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Fri Apr 27 00:00:00 EDT 2018

Inflammasome-driven catecholamine catabolism in macrophages blunts lipolysis during ageing.

Nature

Camell CD,Sander J,Spadaro O,Lee A,Nguyen KY,Wing A,Goldberg EL,Youm YH,Brown CW,Elsworth J,Rodeheffer MS,Schultze JL,Dixit VD

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2017

Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Nature cell biology

Asada N,Kunisaki Y,Pierce H,Wang Z,Fernandez NF,Birbrair A,Ma'ayan A,Frenette PS

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Anti-tumor immunity of BAM-SiPc-mediated vascular photodynamic therapy in a BALB/c mouse model.

Cellular & molecular immunology

Yeung HY,Lo PC,Ng DK,Fong WP

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Wed Feb 01 00:00:00 EST 2017

Combined antiangiogenic and anti-PD-L1 therapy stimulates tumor immunity through HEV formation.

Science translational medicine

Allen E,Jabouille A,Rivera LB,Lodewijckx I,Missiaen R,Steri V,Feyen K,Tawney J,Hanahan D,Michael IP,Bergers G

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Wed Apr 12 00:00:00 EDT 2017

Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma.

The Journal of experimental medicine

Takahashi T,Asano Y,Sugawara K,Yamashita T,Nakamura K,Saigusa R,Ichimura Y,Toyama T,Taniguchi T,Akamata K,Noda S,Yoshizaki A,Tsuruta D,Trojanowska M,Sato S

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon Apr 03 00:00:00 EDT 2017

Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma.

The Journal of experimental medicine

Takahashi T,Asano Y,Sugawara K,Yamashita T,Nakamura K,Saigusa R,Ichimura Y,Toyama T,Taniguchi T,Akamata K,Noda S,Yoshizaki A,Tsuruta D,Trojanowska M,Sato S

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon Apr 03 00:00:00 EDT 2017

ADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.

RNA biology

Ben-Shoshan SO,Kagan P,Sultan M,Barabash Z,Dor C,Jacob-Hirsch J,Harmelin A,Pappo O,Marcu-Malina V,Ben-Ari Z,Amariglio N,Rechavi G,Goldstein I,Safran M

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu May 04 00:00:00 EDT 2017

EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.

Nature communications

Béguelin W,Rivas MA,Calvo Fernández MT,Teater M,Purwada A,Redmond D,Shen H,Challman MF,Elemento O,Singh A,Melnick AM

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2017

DNA-binding of the Tet-transactivator curtails antigen-induced lymphocyte activation in mice.

Nature communications

Ottina E,Peperzak V,Schoeler K,Carrington E,Sgonc R,Pellegrini M,Preston S,Herold MJ,Strasser A,Villunger A

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Wed Oct 18 00:00:00 EDT 2017

Integrating Enhancer Mechanisms to Establish a Hierarchical Blood Development Program.

Cell reports

Mehta C,Johnson KD,Gao X,Ong IM,Katsumura KR,McIver SC,Ranheim EA,Bresnick EH

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2017

The Interleukin (IL)-1R1 pathway is a critical negative regulator of PyMT-mediated mammary tumorigenesis and pulmonary metastasis.

Oncoimmunology

Dagenais M,Dupaul-Chicoine J,Douglas T,Champagne C,Morizot A,Saleh M

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.

JCI insight

Azzam KM,Madenspacher JH,Cain DW,Lai L,Gowdy KM,Rai P,Janardhan K,Clayton N,Cunningham W,Jensen H,Patel PS,Kearney JF,Taylor GA,Fessler MB

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis.

PloS one

Komegae EN,Souza TA,Grund LZ,Lima C,Lopes-Ferreira M

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from polyposis initiation.

International journal of cancer

Allaire JM,Roy SA,Ouellet C,Lemieux É,Jones C,Paquet M,Boudreau F,Perreault N

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Wed Jun 01 00:00:00 EDT 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

This paper is the winner of an SFB Award in the Hospital Intern, Residency category: Peptide biomaterials raising adaptive immune responses in wound healing contexts.

Journal of biomedical materials research. Part A

Vigneswaran Y,Han H,De Loera R,Wen Y,Zhang X,Sun T,Mora-Solano C,Collier JH

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Mon Aug 01 00:00:00 EDT 2016

Postnatal lethality and abnormal development of foregut and spleen in Ndrg4 mutant mice.

Biochemical and biophysical research communications

Qu X,Li J,Baldwin HS

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Fri Feb 12 00:00:00 EST 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

p21^Cip1 plays a critical role in the physiological adaptation to fasting through activation of PPARα.

Scientific reports

Lopez-Guadamillas E,Fernandez-Marcos PJ,Pantoja C,Muñoz-Martin M,Martínez D,Gómez-López G,Campos-Olivas R,Valverde AM,Serrano M

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Mon Oct 10 00:00:00 EDT 2016

Mitotic History Reveals Distinct Stem Cell Populations and Their Contributions to Hematopoiesis.

Cell reports

Säwén P,Lang S,Mandal P,Rossi DJ,Soneji S,Bryder D

16-0452 was used in Flow cytometry/Cell sorting to characterize hematopoietic stem cell and progenitor proliferation dynamics in steady state and following several types of induced stress.

Tue Mar 29 00:00:00 EDT 2016

Aging-associated inflammation promotes selection for adaptive oncogenic events in B cell progenitors.

The Journal of clinical investigation

Henry CJ,Casás-Selves M,Kim J,Zaberezhnyy V,Aghili L,Daniel AE,Jimenez L,Azam T,McNamee EN,Clambey ET,Klawitter J,Serkova NJ,Tan AC,Dinarello CA,DeGregori J

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.

Proceedings of the National Academy of Sciences of the United States of America

Wu L,Li N,Zhang M,Xue SL,Cassady K,Lin Q,Riggs AD,Zeng D

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Tue Dec 29 00:00:00 EST 2015

MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.

Proceedings of the National Academy of Sciences of the United States of America

Wu L,Li N,Zhang M,Xue SL,Cassady K,Lin Q,Riggs AD,Zeng D

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Tue Dec 29 00:00:00 EST 2015

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.

Immunity & ageing : I & A

Matsuda T,Yanase S,Takaoka A,Maruyama M

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Mon Mar 02 00:00:00 EST 2015

B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses.

Journal of immunology (Baltimore, Md. : 1950)

Pone EJ,Lam T,Lou Z,Wang R,Chen Y,Liu D,Edinger AL,Xu Z,Casali P

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Murine Fibroblastic Reticular Cells From Lymph Node Interact With CD4+ T Cells Through CD40-CD40L.

Transplantation

Nakayama Y,Brinkman CC,Bromberg JS

16-0452 was used in Flow cytometry/Cell sorting to elucidate the mechanisms facilitating bidirectional communication between fibroblastic reticular cells and CD4(+) T cells.

Sat Aug 01 00:00:00 EDT 2015

Comparison of tamoxifen and letrozole response in mammary preneoplasia of ER and aromatase overexpressing mice defines an immune-associated gene signature linked to tamoxifen resistance.

Carcinogenesis

Dabydeen SA,Kang K,Díaz-Cruz ES,Alamri A,Axelrod ML,Bouker KB,Al-Kharboosh R,Clarke R,Hennighausen L,Furth PA

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Thu Jan 01 00:00:00 EST 2015

Limited miR-17-92 overexpression drives hematologic malignancies.

Leukemia research

Danielson LS,Reavie L,Coussens M,Davalos V,Castillo-Martin M,Guijarro MV,Coffre M,Cordon-Cardo C,Aifantis I,Ibrahim S,Liu C,Koralov SB,Hernando E

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Sun Mar 01 00:00:00 EST 2015

Limited miR-17-92 overexpression drives hematologic malignancies.

Leukemia research

Danielson LS,Reavie L,Coussens M,Davalos V,Castillo-Martin M,Guijarro MV,Coffre M,Cordon-Cardo C,Aifantis I,Ibrahim S,Liu C,Koralov SB,Hernando E

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Sun Mar 01 00:00:00 EST 2015

Endothelial Gata5 transcription factor regulates blood pressure.

Nature communications

Messaoudi S,He Y,Gutsol A,Wight A,Hébert RL,Vilmundarson RO,Makrigiannis AP,Chalmers J,Hamet P,Tremblay J,McPherson R,Stewart AFR,Touyz RM,Nemer M

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Mon Nov 30 00:00:00 EST 2015

Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.

Nature communications

Singh NK,Kotla S,Dyukova E,Traylor JG,Orr AW,Chernoff J,Marion TN,Rao GN

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Wed Jun 24 00:00:00 EDT 2015

Essential role for autophagy in the maintenance of immunological memory against influenza infection.

Nature medicine

Chen M,Hong MJ,Sun H,Wang L,Shi X,Gilbert BE,Corry DB,Kheradmand F,Wang J

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu May 01 00:00:00 EDT 2014

Imaging murine NALT following intranasal immunization with flagellin-modified circumsporozoite protein malaria vaccines.

Mucosal immunology

Nacer A,Carapau D,Mitchell R,Meltzer A,Shaw A,Frevert U,Nardin EH

16-0452 was used in Flow cytometry/Cell sorting to understand the intranasal immunisation-elicited immune response in nasopharynx-associated lymphoid tissue.

Sat Mar 01 00:00:00 EST 2014

Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response.

Journal of immunology (Baltimore, Md. : 1950)

Park KS,Bayles I,Szlachta-McGinn A,Paul J,Boiko J,Santos P,Liu J,Wang Z,Borghesi L,Milcarek C

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Sat Nov 01 00:00:00 EDT 2014

IκBε is a key regulator of B cell expansion by providing negative feedback on cRel and RelA in a stimulus-specific manner.

Journal of immunology (Baltimore, Md. : 1950)

Alves BN,Tsui R,Almaden J,Shokhirev MN,Davis-Turak J,Fujimoto J,Birnbaum H,Ponomarenko J,Hoffmann A

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-κB signalling.

PloS one

Kasama Y,Mizukami T,Kusunoki H,Peveling-Oberhag J,Nishito Y,Ozawa M,Kohara M,Mizuochi T,Tsukiyama-Kohara K

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Mon Dec 22 00:00:00 EST 2014

B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-κB signalling.

PloS one

Kasama Y,Mizukami T,Kusunoki H,Peveling-Oberhag J,Nishito Y,Ozawa M,Kohara M,Mizuochi T,Tsukiyama-Kohara K

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Mon Dec 22 00:00:00 EST 2014

Rhof promotes murine marginal zone B cell development.

Nagoya journal of medical science

Kishimoto M,Matsuda T,Yanase S,Katsumi A,Suzuki N,Ikejiri M,Takagi A,Ikawa M,Kojima T,Kunishima S,Kiyoi H,Naoe T,Matsushita T,Maruyama M

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

Selective regulation of lymphopoiesis and leukemogenesis by individual zinc fingers of Ikaros.

Nature immunology

Schjerven H,McLaughlin J,Arenzana TL,Frietze S,Cheng D,Wadsworth SE,Lawson GW,Bensinger SJ,Farnham PJ,Witte ON,Smale ST

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

Natural IgG antibodies provide innate protection against ficolin-opsonized bacteria.

The EMBO journal

Panda S,Zhang J,Tan NS,Ho B,Ding JL

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Wed Nov 13 00:00:00 EST 2013

Reciprocal effects of rab7 deletion in activated and neglected T cells.

Autophagy

Roy SG,Stevens MW,So L,Edinger AL

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis.

eLife

Olive V,Sabio E,Bennett MJ,De Jong CS,Biton A,McGann JC,Greaney SK,Sodir NM,Zhou AY,Balakrishnan A,Foth M,Luftig MA,Goga A,Speed TP,Xuan Z,Evan GI,Wan Y,Minella AC,He L

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Tue Oct 15 00:00:00 EDT 2013

Cbx4 regulates the proliferation of thymic epithelial cells and thymus function.

Development (Cambridge, England)

Liu B,Liu YF,Du YR,Mardaryev AN,Yang W,Chen H,Xu ZM,Xu CQ,Zhang XR,Botchkarev VA,Zhang Y,Xu GL

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Fri Feb 01 00:00:00 EST 2013

Cbx4 regulates the proliferation of thymic epithelial cells and thymus function.

Development (Cambridge, England)

Liu B,Liu YF,Du YR,Mardaryev AN,Yang W,Chen H,Xu ZM,Xu CQ,Zhang XR,Botchkarev VA,Zhang Y,Xu GL

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Fri Feb 01 00:00:00 EST 2013

Arrayed lentiviral barcoding for quantification analysis of hematopoietic dynamics.

Stem cells (Dayton, Ohio)

Grosselin J,Sii-Felice K,Payen E,Chretien S,Tronik-Le Roux D,Leboulch P

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

Chemokine CXCL16 suppresses liver metastasis of colorectal cancer via augmentation of tumor-infiltrating natural killer T cells in a murine model.

Oncology reports

Kee JY,Ito A,Hojo S,Hashimoto I,Igarashi Y,Tsukada K,Irimura T,Shibahara N,Nakayama T,Yoshie O,Sakurai H,Saiki I,Koizumi K

16-0452 was used in Flow cytometry/Cell sorting to investigate the role of cancer-derived CXCL16 and the possibility of gene therapy using CXCL16 for the treatment of cancer.

Fri Mar 01 00:00:00 EST 2013

Neuronal and nonneuronal cholinergic structures in the mouse gastrointestinal tract and spleen.

The Journal of comparative neurology

Gautron L,Rutkowski JM,Burton MD,Wei W,Wan Y,Elmquist JK

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunofluorescence

Fri Nov 01 00:00:00 EDT 2013

TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.

PLoS genetics

Gómez-Herreros F,Romero-Granados R,Zeng Z,Alvarez-Quilón A,Quintero C,Ju L,Umans L,Vermeire L,Huylebroeck D,Caldecott KW,Cortés-Ledesma F

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase.

Journal of immunology (Baltimore, Md. : 1950)

Hasham MG,Snow KJ,Donghia NM,Branca JA,Lessard MD,Stavnezer J,Shopland LS,Mills KD

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Fri Feb 25 00:00:00 EST 2011

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Immunohistochemistry

Fri Feb 25 00:00:00 EST 2011

Sensitized phenotypic screening identifies gene dosage sensitive region on chromosome 11 that predisposes to disease in mice.

EMBO molecular medicine

Ermakova O,Piszczek L,Luciani L,Cavalli FM,Ferreira T,Farley D,Rizzo S,Paolicelli RC,Al-Banchaabouchi M,Nerlov C,Moriggl R,Luscombe NM,Gross C

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2011

PAD4-mediated neutrophil extracellular trap formation is not required for immunity against influenza infection.

PloS one

Hemmers S,Teijaro JR,Arandjelovic S,Mowen KA

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2011

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

p27 deficiency cooperates with Bcl-2 but not Bax to promote T-cell lymphoma.

PloS one

Cheng N,van de Wetering CI,Knudson CM

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Wed Apr 02 00:00:00 EDT 2008

Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing.

Molecular and cellular biology

Bai L,Chen Y,He Y,Dai X,Lin X,Wen R,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2007

Essential role of phospholipase C gamma 2 in early B-cell development and Myc-mediated lymphomagenesis.

Molecular and cellular biology

Wen R,Chen Y,Bai L,Fu G,Schuman J,Dai X,Zeng H,Yang C,Stephan RP,Cleveland JL,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2006

Essential role of phospholipase C gamma 2 in early B-cell development and Myc-mediated lymphomagenesis.

Molecular and cellular biology

Wen R,Chen Y,Bai L,Fu G,Schuman J,Dai X,Zeng H,Yang C,Stephan RP,Cleveland JL,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2006

Distinct roles of phosphoinositide-3 kinase and phospholipase Cgamma2 in B-cell receptor-mediated signal transduction.

Molecular and cellular biology

Dai X,Chen Y,Schuman J,Hua Z,Adamson JW,Wen R,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2006

A role for brain-derived neurotrophic factor in B cell development.

Journal of neuroimmunology

Schuhmann B,Dietrich A,Sel S,Hahn C,Klingenspor M,Lommatzsch M,Gudermann T,Braun A,Renz H,Nockher WA

Wed Jun 01 00:00:00 EDT 2005

Impaired immune responses and B-cell proliferation in mice lacking the Id3 gene.

Molecular and cellular biology

Pan L,Sato S,Frederick JP,Sun XH,Zhuang Y

Published figure using CD45R (B220) monoclonal antibody (Product # 16-0452-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; loc; Ly-5; LY5; Lyt-4; PTPRC; T200

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UniProt ID: (Human) P08575, (Mouse) P06800

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Entrez Gene ID: (Human) 5788, (Mouse) 19264

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding phosphoprotein phosphatase activity heparin binding hydrolase activity phosphatase activity heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation activation of MAPK activity positive regulation of T cell mediated cytotoxicity positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin leukocyte cell-cell adhesion response to gamma radiation B cell differentiation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion T cell proliferation positive regulation of MAPK cascade positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes negative regulation of peptidyl-tyrosine phosphorylation regulation of B cell receptor signaling pathway regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway

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CD45R (B220) Monoclonal Antibody (RA3-6B2), Functional Grade, eBioscience™

CD45R (B220) Antibody (16-0452-82) in Flow

Product Details

16-0452-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

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Type

Clone

Conjugate

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