Invitrogen

CD45R (B220) Monoclonal Antibody (RA3-6B2), APC-eFluor™ 780, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

71 Published Figures

135 References

View all (105) B220 (CD45R) antibodies

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Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD45R (B220) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 73

CD45R (B220) Antibody (47-0452-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.25 µg of Rat IgG2a kappa Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or 0.25 µg of Anti-Human/Mouse CD45R (B220) APC-eFluor® 780 (right). Total viable cells were used for analysis.

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Product Details

47-0452-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 134 publications 134 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bacteria, Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RA3-6B2

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1518810

Product Specific Information

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Platelet factors attenuate inflammation and rescue cognition in ageing.

Nature

Schroer AB,Ventura PB,Sucharov J,Misra R,Chui MKK,Bieri G,Horowitz AM,Smith LK,Encabo K,Tenggara I,Couthouis J,Gross JD,Chan JM,Luke A,Villeda SA

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

B cell class switch recombination is regulated by DYRK1A through MSH6 phosphorylation.

Nature communications

Stoler-Barak L,Harris E,Peres A,Hezroni H,Kuka M,Di Lucia P,Grenov A,Gurwicz N,Kupervaser M,Yip BH,Iannacone M,Yaari G,Crispino JD,Shulman Z

47-0452-82 was used in Flow cytometry/Cell sorting to demonstrate DYRK1A-mediated biological mechanisms of B cell immune responses that may be used for therapeutic manipulation in antibody-mediated autoimmunity.

Thu Mar 16 00:00:00 EDT 2023

Stromal niche inflammation mediated by IL-1 signalling is a targetable driver of haematopoietic ageing.

Nature cell biology

Mitchell CA,Verovskaya EV,Calero-Nieto FJ,Olson OC,Swann JW,Wang X,Hérault A,Dellorusso PV,Zhang SY,Svendsen AF,Pietras EM,Bakker ST,Ho TT,Göttgens B,Passegué E

47-0452-82 was used in Flow cytometry/Cell sorting to characterize the inflammatory milieu that drives both niche and haematopoietic remodelling.

Sun Jan 01 00:00:00 EST 2023

The histone H2B ubiquitination regulator Wac is essential for plasma cell differentiation.

FEBS letters

Li Y,Ruan GX,Chen W,Huang H,Zhang R,Wang J,Ouyang Y,Zhu Z,Meng L,Wang R,Huo J,Xu S,Ou X

47-0452-82 was used in Flow Cytometry to demonstrate an essential role of Wac-mediated ubH2B in PC differentiation and shed light on the epigenetic mechanisms underlying this process.

Sat Jul 01 00:00:00 EDT 2023

Secretory MPP3 reinforce myeloid differentiation trajectory and amplify myeloid cell production.

The Journal of experimental medicine

Kang YA,Paik H,Zhang SY,Chen JJ,Olson OC,Mitchell CA,Collins A,Swann JW,Warr MR,Fan R,Passegué E

47-0452-82 was used in Flow Cytometry to identify a novel regulatory function for a secretory MPP3 subset that controls myeloid differentiation through lineage-priming and cytokine production and acts as a self-reinforcing amplification compartment in inflammatory stress and disease conditions.

Mon Aug 07 00:00:00 EDT 2023

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

47-0452-82 was used in Flow cytometry/Cell sorting to show that influenza infection rapidly induces EFRs, generating protective antibodies via Toll-like receptor (TLR)-mediated mechanisms that are both B cell intrinsic and extrinsic.

Wed Jul 05 00:00:00 EDT 2023

Niche-expressed Galectin-1 is involved in pre-B acute lymphoblastic leukemia relapse through pre-B cell receptor activation.

iScience

Pelletier J,Balzano M,Destin J,Montersino C,Delahaye MC,Marchand T,Bailly AL,Bardin F,Coppin E,Goubard A,Castellano R,de Bruijn MJW,Rip J,Collette Y,Dubreuil P,Tarte K,Broccardo C,Hendriks RW,Schiff C,Vey N,Aurrand-Lions M,Mancini SJC

47-0452-82 was used in Flow cytometry/Cell sorting to show that non-cell autonomous signals transmitted by BM niches represent promising targets to improve B-ALL patient survival.

Fri Apr 21 00:00:00 EDT 2023

CD153/CD30 signaling promotes age-dependent tertiary lymphoid tissue expansion and kidney injury.

The Journal of clinical investigation

Sato Y,Oguchi A,Fukushima Y,Masuda K,Toriu N,Taniguchi K,Yoshikawa T,Cui X,Kondo M,Hosoi T,Komidori S,Shimizu Y,Fujita H,Jiang L,Kong Y,Yamanashi T,Seita J,Yamamoto T,Toyokuni S,Hamazaki Y,Hattori M,Yoshikai Y,Boor P,Floege J,Kawamoto H,Murakawa Y,Minato N,Yanagita M

47-0452-82 was used in Flow Cytometry to reveal a previously unappreciated function of CD153/CD30 signaling in TLT formation and propose targeting the CD153/CD30 signaling pathway as a therapeutic target for slowing kidney disease progression.

Tue Jan 18 00:00:00 EST 2022

Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.

JCI insight

Zhong L,Yao L,Holdreith N,Yu W,Gui T,Miao Z,Elkaim Y,Li M,Gong Y,Pacifici M,Maity A,Busch TM,Joeng KS,Cengel K,Seale P,Tong W,Qin L

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Apr 08 00:00:00 EDT 2022

Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma.

Cell death & disease

Sun Q,Wang Y,Ji H,Sun X,Xie S,Chen L,Li S,Zeng W,Chen R,Tang Q,Zuo J,Hou L,Hosaka K,Lu Y,Liu Y,Ye Y,Yang Y

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Aug 19 00:00:00 EDT 2022

Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis.

Science advances

Ma L,Tang Q,Gao X,Lee J,Lei R,Suzuki M,Zheng D,Ito K,Frenette PS,Dawlaty MM

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis.

Science advances

Ma L,Tang Q,Gao X,Lee J,Lei R,Suzuki M,Zheng D,Ito K,Frenette PS,Dawlaty MM

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Maternal regulation of biliary disease in neonates via gut microbial metabolites.

Nature communications

Jee JJ,Yang L,Shivakumar P,Xu PP,Mourya R,Thanekar U,Yu P,Zhu Y,Pan Y,Wang H,Duan X,Ye Y,Wang B,Jin Z,Liu Y,Cao Z,Watanabe-Chailland M,Romick-Rosendale LE,Wagner M,Fei L,Luo Z,Ollberding NJ,Tang ST,Bezerra JA

47-0452-82 was used in Flow Cytometry to study the relationship between microbial metabolites and biliary disease in offspring.

Mon Jan 10 00:00:00 EST 2022

Sphk2 deletion is involved in structural abnormalities and Th17 response but does not aggravate colon inflammation induced by sub-chronic stress.

Scientific reports

Martín-Hernández D,Gutiérrez IL,González-Prieto M,MacDowell KS,Robledo-Montaña J,Tendilla-Beltrán H,Calleja-Rodríguez N,Bris ÁG,Ulecia-Morón C,Moreno B,Caso JR,García-Bueno B,Rodrigues-Mascarenhas S,Marín-Jiménez I,Leza JC,Menchén L

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2022

Assessing the Presence of Hematopoietic Stem and Progenitor Cells in Mouse Spleen.

Bio-protocol

Marié IJ,Brambilla L,Levy DE

47-0452-82 was used in Flow cytometry/Cell sorting to describe an analogous procedure using hematopoietic material from spleen, allowing detection of functional progenitors and/or stem cells in the spleen that can occur under certain pathologies.

Sun Jun 05 00:00:00 EDT 2022

The microbiota regulates hematopoietic stem cell fate decisions by controlling iron availability in bone marrow.

Cell stem cell

Zhang D,Gao X,Li H,Borger DK,Wei Q,Yang E,Xu C,Pinho S,Frenette PS

47-0452-82 was used in Flow cytometry/Cell sorting to reveal an intricate interplay between the microbiota, macrophages, and iron, and their essential roles in regulating critical HSC fate decisions under stress.

Thu Feb 03 00:00:00 EST 2022

YTHDF2 suppresses the plasmablast genetic program and promotes germinal center formation.

Cell reports

Grenov A,Hezroni H,Lasman L,Hanna JH,Shulman Z

47-0452-82 was used in Flow Cytometry to reveal how post-transcriptional suppression of gene expression directs appropriate B cell fate commitment during initiation of the adaptive immune response.

Tue May 03 00:00:00 EDT 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system.

Computational and structural biotechnology journal

Shen Y,Jiang L,Iyer VS,Raposo B,Dubnovitsky A,Boddul SV,Kasza Z,Wermeling F

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Wed Nov 10 00:00:00 EST 2021

Defining the Mechanistic Correlates of Protection Conferred by Whole-Cell Vaccination against Pseudomonas aeruginosa Acute Murine Pneumonia.

Infection and immunity

Sen-Kilic E,Blackwood CB,Huckaby AB,Horspool AM,Weaver KL,Malkowski AC,Witt WT,Bevere JR,Damron FH,Barbier M

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2021

Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo.

Stem cell research

Sudo K,Yamazaki S,Wilkinson AC,Nakauchi H,Nakamura Y

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.

eLife

Hill DL,Whyte CE,Innocentin S,Lee JL,Dooley J,Wang J,James EA,Lee JC,Kwok WW,Zand MS,Liston A,Carr EJ,Linterman MA

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

Metabolic preconditioning in CD4+ T cells restores inducible immune tolerance in lupus-prone mice.

JCI insight

Wilson CS,Stocks BT,Hoopes EM,Rhoads JP,McNew KL,Major AS,Moore DJ

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Oct 08 00:00:00 EDT 2021

The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation.

Nature communications

Shang M,Yang H,Yang R,Chen T,Fu Y,Li Y,Fang X,Zhang K,Zhang J,Li H,Cao X,Gu J,Xiao J,Zhang Q,Liu X,Yu Q,Wang T

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Mon Mar 29 00:00:00 EDT 2021

Type 2 diabetic mice enter a state of spontaneous hibernation-like suspended animation following accumulation of uric acid.

The Journal of biological chemistry

Zhao Y,Cheng R,Zhao Y,Ge W,Yang Y,Ding Z,Xu X,Wang Z,Wu Z,Zhang J

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Synergistic immunotherapy of glioblastoma by dual targeting of IL-6 and CD40.

Nature communications

Yang F,He Z,Duan H,Zhang D,Li J,Yang H,Dorsey JF,Zou W,Nabavizadeh SA,Bagley SJ,Abdullah K,Brem S,Zhang L,Xu X,Byrne KT,Vonderheide RH,Gong Y,Fan Y

47-0452-82 was used in Flow Cytometry to investigate if an antibody cocktail-based immunotherapy that combines checkpoint blockade with dual-targeting of IL-6 and CD40 may offer exciting opportunities for glioblastoma and other solid tumors.

Tue Jun 08 00:00:00 EDT 2021

Regulatory T cells function in established systemic inflammation and reverse fatal autoimmunity.

Nature immunology

Hu W,Wang ZM,Feng Y,Schizas M,Hoyos BE,van der Veeken J,Verter JG,Bou-Puerto R,Rudensky AY

47-0452-82 was used in flow cytometry to show that Treg cells are functional in settings of established broad-spectrum systemic inflammation and are capable of affording sustained reset of immune homeostasis.

Wed Sep 01 00:00:00 EDT 2021

The impact of stress on the transcriptomic signature of iNKT1 cells.

Biochemistry and biophysics reports

Papadogianni G,Ravens I,Hassan A,Dittrich-Breiholz O,Bernhardt G,Georgiev H

47-0452-82 was used in Flow Cytometry to conclude that to avoid erroneous conclusions, a comparison of expression profiles must take into consideration the history of cell preparation.

Wed Dec 01 00:00:00 EST 2021

Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis.

Immunity

Tummers B,Mari L,Guy CS,Heckmann BL,Rodriguez DA,Rühl S,Moretti J,Crawford JC,Fitzgerald P,Kanneganti TD,Janke LJ,Pelletier S,Blander JM,Green DR

47-0452-82 was used in Flow Cytometry to suggest an in vivo role of the inflammatory RIPK1-caspase-8-FADD (FADDosome) complex and reveal a FADD-independent inflammatory role of caspase-8 that involves activation of an inflammasome.

Tue Jun 16 00:00:00 EDT 2020

SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation.

Immunity

Lederer K,Castaño D,Gómez Atria D,Oguin TH,Wang S,Manzoni TB,Muramatsu H,Hogan MJ,Amanat F,Cherubin P,Lundgreen KA,Tam YK,Fan SHY,Eisenlohr LC,Maillard I,Weissman D,Bates P,Krammer F,Sempowski GD,Pardi N,Locci M

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Long-term ex vivo expansion of mouse hematopoietic stem cells.

Nature protocols

Wilkinson AC,Ishida R,Nakauchi H,Yamazaki S

47-0452 was used in Flow cytometry/Cell sorting to describe a simple platform for the expansion of functional mouse hematopoietic stem cells ex vivo for >1 month under fully defined albumin-free conditions.

Sat Feb 01 00:00:00 EST 2020

Snai2 Maintains Bone Marrow Niche Cells by Repressing Osteopontin Expression.

Developmental cell

Wei Q,Nakahara F,Asada N,Zhang D,Gao X,Xu C,Alfieri A,Brodin NP,Zimmerman SE,Mar JC,Guha C,Guo W,Frenette PS

47-0452-82 was used in Flow Cytometry to conclude that SNAI2 is a critical regulator of the transcriptional network maintaining MSPCs by the suppression of osteopontin expression.

Mon Jun 08 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Myeloid Cell CK2 Regulates Inflammation and Resistance to Bacterial Infection.

Frontiers in immunology

Larson SR,Bortell N,Illies A,Crisler WJ,Matsuda JL,Lenz LL

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

Myeloid Cell CK2 Regulates Inflammation and Resistance to Bacterial Infection.

Frontiers in immunology

Larson SR,Bortell N,Illies A,Crisler WJ,Matsuda JL,Lenz LL

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity.

Frontiers in immunology

Myers DR,Abram CL,Wildes D,Belwafa A,Welsh AMN,Schulze CJ,Choy TJ,Nguyen T,Omaque N,Hu Y,Singh M,Hansen R,Goldsmith MA,Quintana E,Smith JAM,Lowell CA

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Mon Jun 14 00:00:00 EDT 2021

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

REV7 is required for processing AID initiated DNA lesions in activated B cells.

Nature communications

Yang D,Sun Y,Chen J,Zhang Y,Fan S,Huang M,Xie X,Cai Y,Shang Y,Gui T,Sun L,Hu J,Dong J,Yeap LS,Wang X,Xiao W,Meng FL

47-0452 was used in Flow cytometry/Cell sorting to identify that REV7-depedent TLS across UNG-processed apurinic/apyrimidinic sites is required for cell survival upon AID/APOBEC deamination.

Thu Jun 04 00:00:00 EDT 2020

B7-CD28 co-stimulation modulates central tolerance via thymic clonal deletion and Treg generation through distinct mechanisms.

Nature communications

Watanabe M,Lu Y,Breen M,Hodes RJ

47-0452 was used in Flow cytometry/Cell sorting to reveal a function of B7-CD28 co-stimulation in shaping the T cell repertoire and limiting autoimmunity through both thymic clonal deletion and Treg cell generation.

Tue Dec 08 00:00:00 EST 2020

TLR9 Sensing of Self-DNA Controls Cell-Mediated Immunity to Listeria Infection via Rapid Conversion of Conventional CD4⁺ T Cells to T_reg.

Cell reports

Dolina JS,Lee J,Griswold RQ,Labarta-Bajo L,Kannan S,Greenbaum JA,Bahia El Idrissi N,Pont MJ,Croft M,Schoenberger SP

47-0452 was used in Flow cytometry/Cell sorting to resolve long-standing questions regarding the conditional requirement for TH amongst pathogens and reveal a remarkable degree of plasticity in the function of CD4+ T cells, which can be quickly converted to Tregin vivo by infection-mediated immune modulation.

Tue Apr 07 00:00:00 EDT 2020

Heterogeneity in old fibroblasts is linked to variability in reprogramming and wound healing.

Nature

Mahmoudi S,Mancini E,Xu L,Moore A,Jahanbani F,Hebestreit K,Srinivasan R,Li X,Devarajan K,Prélot L,Ang CE,Shibuya Y,Benayoun BA,Chang ALS,Wernig M,Wysocka J,Longaker MT,Snyder MP,Brunet A

47-0452 was used in Flow cytometry/Cell sorting to show that this variability may reflect distinct stochastic ageing trajectories between individuals, and could help in developing personalized strategies to improve iPS cell generation and wound healing in elderly individuals.

Tue Oct 01 00:00:00 EDT 2019

Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation.

Nature

Wilkinson AC,Ishida R,Kikuchi M,Sudo K,Morita M,Crisostomo RV,Yamamoto R,Loh KM,Nakamura Y,Watanabe M,Nakauchi H,Yamazaki S

47-0452 was used in Flow cytometry/Cell sorting to identify polyvinyl alcohol as a functionally superior replacement for serum albumin that is compatible with good manufacturing practice.

Mon Jul 01 00:00:00 EDT 2019

Toxin-Triggered Interleukin-1 Receptor Signaling Enables Early-Life Discrimination of Pathogenic versus Commensal Skin Bacteria.

Cell host & microbe

Leech JM,Dhariwala MO,Lowe MM,Chu K,Merana GR,Cornuot C,Weckel A,Ma JM,Leitner EG,Gonzalez JR,Vasquez KS,Diep BA,Scharschmidt TC

47-0452-82 was used in Flow cytometry/Cell sorting to study how skin colonization by commensal Staphylococcus epidermidis facilitates immune tolerance preferentially in neonates via induction of antigen-specific regulatory T cells.

Wed Dec 11 00:00:00 EST 2019

Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells.

Nature cell biology

Nakahara F,Borger DK,Wei Q,Pinho S,Maryanovich M,Zahalka AH,Suzuki M,Cruz CD,Wang Z,Xu C,Boulais PE,Ma'ayan A,Greally JM,Frenette PS

47-0452-82 was used in Flow Cytometry to provide insight into the transcriptional regulation of the niche with implications for stem cell-based therapies.

Wed May 01 00:00:00 EDT 2019

TNF-α Coordinates Hematopoietic Stem Cell Survival and Myeloid Regeneration.

Cell stem cell

Yamashita M,Passegué E

47-0452 was used in Flow cytometry/Cell sorting to reveal several TNF-α-mediated pro-survival mechanisms unique to HSCs, highlight an important role for necroptosis in HSC killing, and establish TNF-α as a major pro-survival and pro-regeneration factor for HSCs.

Thu Sep 05 00:00:00 EDT 2019

TCR Affinity Biases Th Cell Differentiation by Regulating CD25, Eef1e1, and Gbp2.

Journal of immunology (Baltimore, Md. : 1950)

Kotov DI,Mitchell JS,Pengo T,Ruedl C,Way SS,Langlois RA,Fife BT,Jenkins MK

47-0452-82 was used in Flow Cytometry to study TCR affinity and influence on regulation of genes in naive T cells that control the differentiation process in cells.

Wed May 01 00:00:00 EDT 2019

Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells.

Frontiers in immunology

Xu J,Pei S,Wang Y,Liu J,Qian Y,Huang M,Zhang Y,Xiao Y

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Wed Oct 14 00:00:00 EDT 2020

Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.

Nature communications

Ramakrishna C,Kujawski M,Chu H,Li L,Mazmanian SK,Cantin EM

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue May 14 00:00:00 EDT 2019

The Hematopoietic Oxidase NOX2 Regulates Self-Renewal of Leukemic Stem Cells.

Cell reports

Adane B,Ye H,Khan N,Pei S,Minhajuddin M,Stevens BM,Jones CL,D'Alessandro A,Reisz JA,Zaberezhnyy V,Gasparetto M,Ho TC,Kelly KK,Myers JR,Ashton JM,Siegenthaler J,Kume T,Campbell EL,Pollyea DA,Becker MW,Jordan CT

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Apr 02 00:00:00 EDT 2019

Mutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction.

Frontiers in immunology

Fenninger F,Han J,Stanwood SR,Nohara LL,Arora H,Choi KB,Munro L,Pfeifer CG,Shanina I,Horwitz MS,Jefferies WA

47-0452 was used in Flow cytometry/Cell sorting to investigate how a mutation in an L-Type calcium channel gene influences T lymphocyte dysfunction.

Thu Oct 29 00:00:00 EDT 2020

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Cell reports

Balzano M,De Grandis M,Vu Manh TP,Chasson L,Bardin F,Farina A,Sergé A,Bidaut G,Charbord P,Hérault L,Bailly AL,Cartier-Michaud A,Boned A,Dalod M,Duprez E,Genever P,Coles M,Bajenoff M,Xerri L,Aurrand-Lions M,Schiff C,Mancini SJC

47-0452 was used in Flow cytometry/Cell sorting to study pro-B cells in their support of stroll cells and their interaction network allowing their retention.

Tue Mar 19 00:00:00 EDT 2019

Peptide:MHCII Tetramer-Based Cell Enrichment for the Study of Epitope-Specific CD4⁺ T Cells.

Current protocols in immunology

Kotov DI,Jenkins MK

47-0452 was used in Flow cytometry/Cell sorting to study polyclonal epitope-specific CD4+ T cells under physiological conditions.

Sat Jun 01 00:00:00 EDT 2019

Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.

Nature medicine

Maryanovich M,Zahalka AH,Pierce H,Pinho S,Nakahara F,Asada N,Wei Q,Wang X,Ciero P,Xu J,Leftin A,Frenette PS

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

B Cells Are the Dominant Antigen-Presenting Cells that Activate Naive CD4⁺ T Cells upon Immunization with a Virus-Derived Nanoparticle Antigen.

Immunity

Hong S,Zhang Z,Liu H,Tian M,Zhu X,Zhang Z,Wang W,Zhou X,Zhang F,Ge Q,Zhu B,Tang H,Hua Z,Hou B

47-0452-82 was used in Flow Cytometry to demonstrate that antigen-specific B cells were the dominant antigen-presenting cells that initiated naive CD4+ T cell activation. Using RNA phage Qβ-derived VLP (Qβ-VLP) as a model antigen.

Tue Oct 16 00:00:00 EDT 2018

Large-Scale Clonal Analysis Resolves Aging of the Mouse Hematopoietic Stem Cell Compartment.

Cell stem cell

Yamamoto R,Wilkinson AC,Ooehara J,Lan X,Lai CY,Nakauchi Y,Pritchard JK,Nakauchi H

47-0452 was used in Flow cytometry/Cell sorting to determine the age-related functional changes to the haematopoietic stem cell compartment.

Thu Apr 05 00:00:00 EDT 2018

Granulocyte-CSF links destructive inflammation and comorbidities in obstructive lung disease.

The Journal of clinical investigation

Tsantikos E,Lau M,Castelino CM,Maxwell MJ,Passey SL,Hansen MJ,McGregor NE,Sims NA,Steinfort DP,Irving LB,Anderson GP,Hibbs ML

47-0452 was used in Flow cytometry/Cell sorting to study the role of blood growth factors in chronic obstructive pulmonary disease.

Fri Jun 01 00:00:00 EDT 2018

IL1RAP potentiates multiple oncogenic signaling pathways in AML.

The Journal of experimental medicine

Mitchell K,Barreyro L,Todorova TI,Taylor SJ,Antony-Debré I,Narayanagari SR,Carvajal LA,Leite J,Piperdi Z,Pendurti G,Mantzaris I,Paietta E,Verma A,Gritsman K,Steidl U

47-0452 was used in Flow cytometry/Cell sorting to show that targeting of interleukin-1 receptor accessory protein via RNA interference, genetic deletion, or antibodies inhibits acute myeloid leukaemia pathogenesis in vitro and in vivo.

Mon Jun 04 00:00:00 EDT 2018

Proapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner.

Frontiers in immunology

Liu R,King A,Bouillet P,Tarlinton DM,Strasser A,Heierhorst J

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Host Tumor Suppressor p18^INK4c Functions as a Potent Cell-Intrinsic Inhibitor of Murine Gammaherpesvirus 68 Reactivation and Pathogenesis.

Journal of virology

Niemeyer BF,Oko LM,Medina EM,Oldenburg DG,White DW,Cool CD,Clambey ET,van Dyk LF

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Thu Mar 15 00:00:00 EDT 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43.

Experimental and therapeutic medicine

Li Z,Zhang S,Cao L,Li W,Ye YC,Shi ZX,Wang ZR,Sun LX,Wang JW,Jia LT,Wang W

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice.

Frontiers in immunology

Perez-Chacon G,Adrados M,Vallejo-Cremades MT,Lefebvre S,Reed JC,Zapata JM

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Oct 25 00:00:00 EDT 2019

ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.

Frontiers in immunology

Glal D,Sudhakar JN,Lu HH,Liu MC,Chiang HY,Liu YC,Cheng CF,Shui JW

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Thu Sep 26 00:00:00 EDT 2019

Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes.

Scientific reports

Osum KC,Burrack AL,Martinov T,Sahli NL,Mitchell JS,Tucker CG,Pauken KE,Papas K,Appakalai B,Spanier JA,Fife BT

47-0452 was used in Flow cytometry/Cell sorting to suggest that insulin-producing Β cells respond to pancreatic inflammation and IFN-γ production by upregulating PD-L1 expression to limit self-reactive T cells.

Tue May 29 00:00:00 EDT 2018

IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate.

eLife

Noviski M,Mueller JL,Satterthwaite A,Garrett-Sinha LA,Brombacher F,Zikherman J

47-0452-82 was used in Flow Cytometry to study how IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate.

Fri Mar 09 00:00:00 EST 2018

Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow.

Nature communications

Xu C,Gao X,Wei Q,Nakahara F,Zimmerman SE,Mar J,Frenette PS

47-0452 was used in Flow cytometry/Cell sorting to characterise the expression signature that distinguishes arterial endothelial cells from sinusoidal endothelial cells.

Fri Jun 22 00:00:00 EDT 2018

Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.

Nature

Hérault A,Binnewies M,Leong S,Calero-Nieto FJ,Zhang SY,Kang YA,Wang X,Pietras EM,Chu SH,Barry-Holson K,Armstrong S,Göttgens B,Passegué E

47-0452 was used in Flow cytometry/Cell sorting to show that in leukaemia, GMP clusters are constantly produced due to lack of termination cytokines that restore haematopoietic stem-cell quiescence.

Thu Apr 06 00:00:00 EDT 2017

Autophagy maintains the metabolism and function of young and old stem cells.

Nature

Ho TT,Warr MR,Adelman ER,Lansinger OM,Flach J,Verovskaya EV,Figueroa ME,Passegué E

47-0452 was used in Flow cytometry/Cell sorting to demonstrate an essential function for autophagy in removing activated mitochondria and controlling oxidative metabolism, thereby maintaining HSC stemness and regenerative potential.

Thu Mar 09 00:00:00 EST 2017

Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Nature cell biology

Asada N,Kunisaki Y,Pierce H,Wang Z,Fernandez NF,Birbrair A,Ma'ayan A,Frenette PS

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma.

The Journal of experimental medicine

Takahashi T,Asano Y,Sugawara K,Yamashita T,Nakamura K,Saigusa R,Ichimura Y,Toyama T,Taniguchi T,Akamata K,Noda S,Yoshizaki A,Tsuruta D,Trojanowska M,Sato S

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Mon Apr 03 00:00:00 EDT 2017

Pms2 and uracil-DNA glycosylases act jointly in the mismatch repair pathway to generate Ig gene mutations at A-T base pairs.

The Journal of experimental medicine

Girelli Zubani G,Zivojnovic M,De Smet A,Albagli-Curiel O,Huetz F,Weill JC,Reynaud CA,Storck S

47-0452 was used in Flow cytometry/Cell sorting to study how U:G mismatches is translated into the single-strand nick required for error prone synthesis.

Mon Apr 03 00:00:00 EDT 2017

Autophagy in Dendritic Cells and B Cells Is Critical for the Inflammatory State of TLR7-Mediated Autoimmunity.

Journal of immunology (Baltimore, Md. : 1950)

Weindel CG,Richey LJ,Mehta AJ,Shah M,Huber BT

47-0452 was used in Flow cytometry/Cell sorting to identify that autophagy in B cells and dendritic cells are key to TLR7-mediated autoimmune disorders.

Wed Feb 01 00:00:00 EST 2017

EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.

Nature communications

Béguelin W,Rivas MA,Calvo Fernández MT,Teater M,Purwada A,Redmond D,Shen H,Challman MF,Elemento O,Singh A,Melnick AM

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2017

DNA-binding of the Tet-transactivator curtails antigen-induced lymphocyte activation in mice.

Nature communications

Ottina E,Peperzak V,Schoeler K,Carrington E,Sgonc R,Pellegrini M,Preston S,Herold MJ,Strasser A,Villunger A

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Wed Oct 18 00:00:00 EDT 2017

The Interleukin (IL)-1R1 pathway is a critical negative regulator of PyMT-mediated mammary tumorigenesis and pulmonary metastasis.

Oncoimmunology

Dagenais M,Dupaul-Chicoine J,Douglas T,Champagne C,Morizot A,Saleh M

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.

JCI insight

Azzam KM,Madenspacher JH,Cain DW,Lai L,Gowdy KM,Rai P,Janardhan K,Clayton N,Cunningham W,Jensen H,Patel PS,Kearney JF,Taylor GA,Fessler MB

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis.

PloS one

Komegae EN,Souza TA,Grund LZ,Lima C,Lopes-Ferreira M

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.

PloS one

Ender F,Wiese AV,Schmudde I,Sun J,Vollbrandt T,König P,Laumonnier Y,Köhl J

47-0452 was used in Flow cytometry/Cell sorting to demonstrate a complex regulation pattern of C5aR1 in the airways, lung tissue and lymph nodes of mice.

Thu Aug 17 00:00:00 EDT 2017

Acute and chronic effects of treatment with mesenchymal stromal cells on LPS-induced pulmonary inflammation, emphysema and atherosclerosis development.

PloS one

Khedoe PPSJ,de Kleijn S,van Oeveren-Rietdijk AM,Plomp JJ,de Boer HC,van Pel M,Rensen PCN,Berbée JFP,Hiemstra PS

47-0452 was used in Flow cytometry/Cell sorting to investigate the immunomodulatory properties of MSC in acute and chronic models of lipopolysaccharide (LPS)-induced inflammation, emphysema and atherosclerosis development.

Thu Oct 12 00:00:00 EDT 2017

Small molecule p300/catenin antagonist enhances hematopoietic recovery after radiation.

PloS one

Zhao Y,Wu K,Nguyen C,Smbatyan G,Melendez E,Higuchi Y,Chen Y,Kahn M

47-0452 was used in Flow cytometry/Cell sorting to report that the small molecule YH250 stimulates hematopoiesis in lethally or sublethally irradiated mice.

Tue Sep 26 00:00:00 EDT 2017

MyD88-dependent pro-interleukin-1β induction in dendritic cells exposed to food-grade synthetic amorphous silica.

Particle and fibre toxicology

Winkler HC,Kornprobst J,Wick P,von Moos LM,Trantakis I,Schraner EM,Bathke B,Hochrein H,Suter M,Naegeli H

47-0452 was used in Flow cytometry/Cell sorting to unexpectedly show that food-grade SAS particles are able to directly initiate the endosomal MyD88-dependent pathogen pattern recognition and signaling pathway in steady-state DCs.

Fri Jun 23 00:00:00 EDT 2017

Group 2 innate lymphoid cells license dendritic cells to potentiate memory TH2 cell responses.

Nature immunology

Halim TY,Hwang YY,Scanlon ST,Zaghouani H,Garbi N,Fallon PG,McKenzie AN

47-0452 was used in Flow cytometry/Cell sorting to investigate the role of ILC2 cells in allergic inflammation, showing that they license dendritic cells to potentiate memory TH2 cell responses.

Fri Jan 01 00:00:00 EST 2016

Chronic interleukin-1 exposure drives haematopoietic stem cells towards precocious myeloid differentiation at the expense of self-renewal.

Nature cell biology

Pietras EM,Mirantes-Barbeito C,Fong S,Loeffler D,Kovtonyuk LV,Zhang S,Lakshminarasimhan R,Chin CP,Techner JM,Will B,Nerlov C,Steidl U,Manz MG,Schroeder T,Passegué E

47-0452 was used in Flow cytometry/Cell sorting to identify a critical regulatory circuit that tailors HSC responses to acute needs, and is likely to underlie deregulated blood homeostasis in chronic inflammation conditions.

Wed Jun 01 00:00:00 EDT 2016

Heterozygous Mutation in IκBNS Leads to Reduced Levels of Natural IgM Antibodies and Impaired Responses to T-Independent Type 2 Antigens.

Frontiers in immunology

Pedersen GK,Ádori M,Stark JM,Khoenkhoen S,Arnold C,Beutler B,Karlsson Hedestam GB

47-0452 was used in Flow cytometry/Cell sorting to identify a role for the atypical IκB protein IκBNS in humoral immunity.

Mon Mar 14 00:00:00 EDT 2016

IL-33-Dependent Group 2 Innate Lymphoid Cells Promote Cutaneous Wound Healing.

The Journal of investigative dermatology

Rak GD,Osborne LC,Siracusa MC,Kim BS,Wang K,Bayat A,Artis D,Volk SW

47-0452 was used in Flow cytometry/Cell sorting to demonstrate that cutaneous injury promotes an IL-33-dependent group 2 ILC (ILC2) response and that abrogation of this response can impair efficient wound closure.

Mon Feb 01 00:00:00 EST 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

Low-Dose Intestinal Trichuris muris Infection Alters the Lung Immune Microenvironment and Can Suppress Allergic Airway Inflammation.

Infection and immunity

Chenery AL,Antignano F,Burrows K,Scheer S,Perona-Wright G,Zaph C

47-0452 was used in Flow cytometry/Cell sorting to study crosstalk between the intestine and lung during disease, showing that low-dose intestinal Trichuris muris infection alters the lung immune microenvironment.

Mon Feb 01 00:00:00 EST 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

p21^Cip1 plays a critical role in the physiological adaptation to fasting through activation of PPARα.

Scientific reports

Lopez-Guadamillas E,Fernandez-Marcos PJ,Pantoja C,Muñoz-Martin M,Martínez D,Gómez-López G,Campos-Olivas R,Valverde AM,Serrano M

47-0452 was used in Flow cytometry/Cell sorting to examine the expression of several tumour suppressors upon fasting in mice.

Mon Oct 10 00:00:00 EDT 2016

Serum Amyloid A3 Secreted by Preosteoclasts Inhibits Parathyroid Hormone-stimulated cAMP Signaling in Murine Osteoblasts.

The Journal of biological chemistry

Choudhary S,Goetjen A,Estus T,Jacome-Galarza CE,Aguila HL,Lorenzo J,Pilbeam C

47-0452 was used in Flow cytometry/Cell sorting to identify the inhibitor of PTH-stimulated osteoblast differentiation.

Fri Feb 19 00:00:00 EST 2016

Aging-associated inflammation promotes selection for adaptive oncogenic events in B cell progenitors.

The Journal of clinical investigation

Henry CJ,Casás-Selves M,Kim J,Zaberezhnyy V,Aghili L,Daniel AE,Jimenez L,Azam T,McNamee EN,Clambey ET,Klawitter J,Serkova NJ,Tan AC,Dinarello CA,DeGregori J

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

B cell autophagy mediates TLR7-dependent autoimmunity and inflammation.

Autophagy

Weindel CG,Richey LJ,Bolland S,Mehta AJ,Kearney JF,Huber BT

47-0452-82 was used in Flow Cytometry to demonstrate that autophagy plays an essential role in TLR7 activation of B cells for the induction of systemic lupus erythematosus.

Mon Apr 18 00:00:00 EDT 2016

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

MHC-mismatched mixed chimerism mediates thymic deletion of cross-reactive autoreactive T cells and prevents insulitis in nonobese diabetic mice.

Journal of immunology (Baltimore, Md. : 1950)

Racine JJ,Zhang M,Wang M,Morales W,Shen C,Zeng D

47-0452 was used in Flow cytometry/Cell sorting to investigate whether mismatched MHC class II in mixed chimeras mediates deletion of cross-reactive autoreactive thymocytes.

Thu Jan 01 00:00:00 EST 2015

Inhibition of the CXCL12/CXCR4 chemokine axis with AMD3100, a CXCR4 small molecule inhibitor, worsens murine hepatic injury.

Hepatology research : the official journal of the Japan Society of Hepatology

Saiman Y,Jiao J,Fiel MI,Friedman SL,Aloman C,Bansal MB

47-0452 was used in Flow cytometry/Cell sorting to suggest that inhibition of the CXCR4/CXCL12 chemokine axis is injurious through modulation of the hepatic inflammatory response.

Wed Jul 01 00:00:00 EDT 2015

Limited miR-17-92 overexpression drives hematologic malignancies.

Leukemia research

Danielson LS,Reavie L,Coussens M,Davalos V,Castillo-Martin M,Guijarro MV,Coffre M,Cordon-Cardo C,Aifantis I,Ibrahim S,Liu C,Koralov SB,Hernando E

47-0452 was used in Flow cytometry/Cell sorting to show limited miR-17-92 overexpression can drive hematologic malignancies.

Sun Mar 01 00:00:00 EST 2015

Endothelial Gata5 transcription factor regulates blood pressure.

Nature communications

Messaoudi S,He Y,Gutsol A,Wight A,Hébert RL,Vilmundarson RO,Makrigiannis AP,Chalmers J,Hamet P,Tremblay J,McPherson R,Stewart AFR,Touyz RM,Nemer M

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Mon Nov 30 00:00:00 EST 2015

Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.

Nature communications

Singh NK,Kotla S,Dyukova E,Traylor JG,Orr AW,Chernoff J,Marion TN,Rao GN

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Wed Jun 24 00:00:00 EDT 2015

Genome Wide Identification of SARS-CoV Susceptibility Loci Using the Collaborative Cross.

PLoS genetics

Gralinski LE,Ferris MT,Aylor DL,Whitmore AC,Green R,Frieman MB,Deming D,Menachery VD,Miller DR,Buus RJ,Bell TA,Churchill GA,Threadgill DW,Katze MG,McMillan L,Valdar W,Heise MT,Pardo-Manuel de Villena F,Baric RS

47-0452 was used in Flow cytometry/Cell sorting to validate the Collaborative Cross platform as a resource for uncovering the genetic contributions of complex traits in microbial diseases, inflammation and viral replication.

Thu Oct 01 00:00:00 EDT 2015

Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality.

Nature communications

Alvarez S,Díaz M,Flach J,Rodriguez-Acebes S,López-Contreras AJ,Martínez D,Cañamero M,Fernández-Capetillo O,Isern J,Passegué E,Méndez J

47-0452 was used in Flow cytometry/Cell sorting to determine that haematopoietic progenitors are particularly sensitive to replication stress.

Mon Oct 12 00:00:00 EDT 2015

Megakaryocytes regulate hematopoietic stem cell quiescence through CXCL4 secretion.

Nature medicine

Bruns I,Lucas D,Pinho S,Ahmed J,Lambert MP,Kunisaki Y,Scheiermann C,Schiff L,Poncz M,Bergman A,Frenette PS

47-0452 was used in Flow cytometry/Cell sorting to indicate that a terminally differentiated cell type derived from HSCs contributes to the HSC niche, directly regulating HSC behavior.

Sat Nov 01 00:00:00 EDT 2014

Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells.

Nature

Flach J,Bakker ST,Mohrin M,Conroy PC,Pietras EM,Reynaud D,Alvarez S,Diolaiti ME,Ugarte F,Forsberg EC,Le Beau MM,Stohr BA,Méndez J,Morrison CG,Passegué E

47-0452 was used in Flow cytometry/Cell sorting to study functional decline in ageing haematopoietic stem cells due to replicative stress.

Thu Aug 14 00:00:00 EDT 2014

Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response.

Journal of immunology (Baltimore, Md. : 1950)

Park KS,Bayles I,Szlachta-McGinn A,Paul J,Boiko J,Santos P,Liu J,Wang Z,Borghesi L,Milcarek C

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Sat Nov 01 00:00:00 EDT 2014

IκBε is a key regulator of B cell expansion by providing negative feedback on cRel and RelA in a stimulus-specific manner.

Journal of immunology (Baltimore, Md. : 1950)

Alves BN,Tsui R,Almaden J,Shokhirev MN,Davis-Turak J,Fujimoto J,Birnbaum H,Ponomarenko J,Hoffmann A

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

CD40 ligand preferentially modulates immune response and enhances protection against influenza virus.

Journal of immunology (Baltimore, Md. : 1950)

Hashem AM,Gravel C,Chen Z,Yi Y,Tocchi M,Jaentschke B,Fan X,Li C,Rosu-Myles M,Pereboev A,He R,Wang J,Li X

47-0452 was used in Flow cytometry/Cell sorting to characterise an increased in vivo load of CD40-targeted Ag upon the secretion of the NP-CD40L fusion protein from adenovirus-infected cells.

Tue Jul 15 00:00:00 EDT 2014

Rhof promotes murine marginal zone B cell development.

Nagoya journal of medical science

Kishimoto M,Matsuda T,Yanase S,Katsumi A,Suzuki N,Ikejiri M,Takagi A,Ikawa M,Kojima T,Kunishima S,Kiyoi H,Naoe T,Matsushita T,Maruyama M

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-κB signalling.

PloS one

Kasama Y,Mizukami T,Kusunoki H,Peveling-Oberhag J,Nishito Y,Ozawa M,Kohara M,Mizuochi T,Tsukiyama-Kohara K

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Mon Dec 22 00:00:00 EST 2014

Establishment of a murine graft-versus-myeloma model using allogeneic stem cell transplantation.

PloS one

Binsfeld M,Beguin Y,Belle L,Otjacques E,Hannon M,Briquet A,Heusschen R,Drion P,Zilberberg J,Bogen B,Baron F,Caers J

47-0452 was used in Flow cytometry/Cell sorting to investigate the anti-myeloma effects of allo-SCT from B10.D2 mice into MHC-matched myeloma-bearing Balb/cJ mice.

Tue Dec 15 00:00:00 EST 2015

HIV-1 Env-specific memory and germinal center B cells in C57BL/6 mice.

Viruses

Soldemo M,Pedersen GK,Karlsson Hedestam GB

47-0452 was used in Flow cytometry/Cell sorting to compare HIV-1 Env immunogenicity and memory in BALB/c and C57BL/6 mice.

Fri Sep 05 00:00:00 EDT 2014

FOXO3A directs a protective autophagy program in haematopoietic stem cells.

Nature

Warr MR,Binnewies M,Flach J,Reynaud D,Garg T,Malhotra R,Debnath J,Passegué E

47-0452 was used in Flow cytometry/Cell sorting to show how FOXO3A directs a protective autophagy program in haematopoietic stem cells.

Thu Feb 21 00:00:00 EST 2013

Ecto-5'-nucleotidase on immune cells protects from adverse cardiac remodeling.

Circulation research

Bönner F,Borg N,Jacoby C,Temme S,Ding Z,Flögel U,Schrader J

47-0452 was used in Flow cytometry/Cell sorting to examine the role of CD73 in cardiac repair and remodelling in mice after reperfused myocardial infarction.

Fri Jul 19 00:00:00 EDT 2013

Reciprocal effects of rab7 deletion in activated and neglected T cells.

Autophagy

Roy SG,Stevens MW,So L,Edinger AL

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

Dual Pten/Tp53 suppression promotes sarcoma progression by activating Notch signaling.

The American journal of pathology

Guijarro MV,Dahiya S,Danielson LS,Segura MF,Vales-Lara FM,Menendez S,Popiolek D,Mittal K,Wei JJ,Zavadil J,Cordon-Cardo C,Pandolfi PP,Hernando E

47-0452 was used in Flow cytometry/Cell sorting to elucidate the mechanisms driving cytogenetically complex sarcoma development.

Sat Jun 01 00:00:00 EDT 2013

TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.

PLoS genetics

Gómez-Herreros F,Romero-Granados R,Zeng Z,Alvarez-Quilón A,Quintero C,Ju L,Umans L,Vermeire L,Huylebroeck D,Caldecott KW,Cortés-Ledesma F

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

N-cadherin in osteolineage cells is not required for maintenance of hematopoietic stem cells.

Blood

Greenbaum AM,Revollo LD,Woloszynek JR,Civitelli R,Link DC

47-0452 was used in Flow cytometry/Cell sorting to investigate whether N-cadherin expression on osteoblast lineage cells regulates HSC function and quiescence, showing that it is not required.

Thu Jul 12 00:00:00 EDT 2012

Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress.

The Journal of experimental medicine

Austin WR,Armijo AL,Campbell DO,Singh AS,Hsieh T,Nathanson D,Herschman HR,Phelps ME,Witte ON,Czernin J,Radu CG

47-0452 was used in Flow cytometry/Cell sorting to investigate a functional link between nucleotide deficiency, replication stress, and two enzymes of the nucleoside salvage pathway: deoxycytidine kinase and thymidine kinase.

Mon Nov 19 00:00:00 EST 2012

The polymorphic pseudokinase ROP5 controls virulence in Toxoplasma gondii by regulating the active kinase ROP18.

PLoS pathogens

Behnke MS,Fentress SJ,Mashayekhi M,Li LX,Taylor GA,Sibley LD

47-0452 was used in Flow cytometry/Cell sorting to demonstrate that the pseudokinase ROP5 controls the virulence of Toxoplasma gondii by blocking IFN-γ mediated clearance in activated macrophages.

Fri Apr 19 00:00:00 EDT 2013

Splenic morphological changes are accompanied by altered baseline immunity in a mouse model of sickle-cell disease.

The American journal of pathology

Szczepanek SM,McNamara JT,Secor ER,Natarajan P,Guernsey LA,Miller LA,Ballesteros E,Jellison E,Thrall RS,Andemariam B

47-0452 was used in Flow cytometry/Cell sorting to investigate the relationship between functional asplenia from infarctions and increased infectious mortality in a mouse model of sickle-cell disease.

Thu Nov 01 00:00:00 EDT 2012

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase.

Journal of immunology (Baltimore, Md. : 1950)

Hasham MG,Snow KJ,Donghia NM,Branca JA,Lessard MD,Stavnezer J,Shopland LS,Mills KD

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2012

Arthritogenic self-reactive CD4+ T cells acquire an FR4hiCD73hi anergic state in the presence of Foxp3+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Martinez RJ,Zhang N,Thomas SR,Nandiwada SL,Jenkins MK,Binstadt BA,Mueller DL

47-0452 was used in Flow cytometry/Cell sorting to suggest that autoimmune arthritis develops in the setting of lymphopenia when Foxp3(+)CD4(+) regulatory T cells are insufficient to functionally inactivate all autoreactive CD4(+) T cells that encounter self-Ag.

Sun Jan 01 00:00:00 EST 2012

Resident cardiac immune cells and expression of the ectonucleotidase enzymes CD39 and CD73 after ischemic injury.

PloS one

Bönner F,Borg N,Burghoff S,Schrader J

47-0452 was used in Flow cytometry/Cell sorting to explore the abundance of CD73 and CD39 on immune cells under control conditions and in response to inflammation.

Thu Aug 30 00:00:00 EDT 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

Expression of the G-CSF receptor in monocytic cells is sufficient to mediate hematopoietic progenitor mobilization by G-CSF in mice.

The Journal of experimental medicine

Christopher MJ,Rao M,Liu F,Woloszynek JR,Link DC

47-0452 was used in Flow cytometry/Cell sorting to suggest a model in which G-CSFR signals in bone marrow monocytic cells inhibit the production of trophic factors required for osteoblast lineage cell maintenance.

Mon Feb 14 00:00:00 EST 2011

Magnetic-based purification of untouched mouse germinal center B cells for ex vivo manipulation and biochemical analysis.

Nature protocols

Cato MH,Yau IW,Rickert RC

47-0452 was used in Flow cytometry/Cell sorting to provide a protocol for a novel magnetic bead separation scheme for the purification of mature B cells for biochemical assessment.

Thu Jun 09 00:00:00 EDT 2011

Blockade of B7-H1 (programmed death ligand 1) enhances humoral immunity by positively regulating the generation of T follicular helper cells.

Journal of immunology (Baltimore, Md. : 1950)

Hams E,McCarron MJ,Amu S,Yagita H,Azuma M,Chen L,Fallon PG

47-0452 was used in Flow cytometry/Cell sorting to demonstrate that the coinhibitory B7-H1/programmed death 1 pathway can limit the expansion of T(FH) cells and constrain Ag-specific Ig responses.

Sun May 15 00:00:00 EDT 2011

Chronic helminth infection promotes immune regulation in vivo through dominance of CD11cloCD103- dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Smith KA,Hochweller K,Hämmerling GJ,Boon L,MacDonald AS,Maizels RM

47-0452 was used in Flow cytometry/Cell sorting to identify that the preferential expansion of a novel population of CD11c(lo) non-plasmacytoid DCs is associated with the pro-regulatory environment of chronic intestinal helminth infection.

Wed Jun 15 00:00:00 EDT 2011

PAD4-mediated neutrophil extracellular trap formation is not required for immunity against influenza infection.

PloS one

Hemmers S,Teijaro JR,Arandjelovic S,Mowen KA

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2011

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

p27 deficiency cooperates with Bcl-2 but not Bax to promote T-cell lymphoma.

PloS one

Cheng N,van de Wetering CI,Knudson CM

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Wed Apr 02 00:00:00 EDT 2008

Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing.

Molecular and cellular biology

Bai L,Chen Y,He Y,Dai X,Lin X,Wen R,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2007

Essential role of phospholipase C gamma 2 in early B-cell development and Myc-mediated lymphomagenesis.

Molecular and cellular biology

Wen R,Chen Y,Bai L,Fu G,Schuman J,Dai X,Zeng H,Yang C,Stephan RP,Cleveland JL,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2006

A role for brain-derived neurotrophic factor in B cell development.

Journal of neuroimmunology

Schuhmann B,Dietrich A,Sel S,Hahn C,Klingenspor M,Lommatzsch M,Gudermann T,Braun A,Renz H,Nockher WA

Wed Jun 01 00:00:00 EDT 2005

Impaired immune responses and B-cell proliferation in mice lacking the Id3 gene.

Molecular and cellular biology

Pan L,Sato S,Frederick JP,Sun XH,Zhuang Y

Published figure using CD45R (B220) monoclonal antibody (Product # 47-0452-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; loc; Ly-5; LY5; Lyt-4; PTPRC; T200

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UniProt ID: (Human) P08575, (Mouse) P06800

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Entrez Gene ID: (Human) 5788, (Mouse) 19264

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding phosphoprotein phosphatase activity heparin binding hydrolase activity phosphatase activity heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation activation of MAPK activity positive regulation of T cell mediated cytotoxicity positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin leukocyte cell-cell adhesion response to gamma radiation B cell differentiation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion T cell proliferation positive regulation of MAPK cascade positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes negative regulation of peptidyl-tyrosine phosphorylation regulation of B cell receptor signaling pathway regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway

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CD45R (B220) Monoclonal Antibody (RA3-6B2), APC-eFluor™ 780, eBioscience™

CD45R (B220) Antibody (47-0452-82) in Flow

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47-0452-82

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