Invitrogen

CD45R (B220) Monoclonal Antibody (RA3-6B2), Super Bright™ 702, eBioscience™

CD45R (B220) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 32

CD45R (B220) Antibody (67-0452-82) in Flow

BALB/c mouse bone marrow cells were stained with CD3 Monoclonal Antibody, APC (Product # 17-0031-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Super Bright 702 (Product # 67-4321-82) (left) or 0.25 µg of CD45R (B220) Monoclonal Antibody, Super Bright 702 (right). Cells in the lymphocyte gate were used for analysis.

CD45R (B220) Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

CD45R (B220) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

67-0452-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 24 publications 24 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 702, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RA3-6B2

Conjugate

Super Bright™ 702 Super Bright™ 702 Super Bright™ 702

Excitation/Emission Max

413/702 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2744882

Product Specific Information

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Platelet factors attenuate inflammation and rescue cognition in ageing.

Nature

Schroer AB,Ventura PB,Sucharov J,Misra R,Chui MKK,Bieri G,Horowitz AM,Smith LK,Encabo K,Tenggara I,Couthouis J,Gross JD,Chan JM,Luke A,Villeda SA

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

O-GlcNAc glycosylation orchestrates fate decision and niche function of bone marrow stromal progenitors.

eLife

Zhang Z,Huang Z,Awad M,Elsalanty M,Cray J,Ball LE,Maynard JC,Burlingame AL,Zeng H,Mansky KC,Ruan HB

67-0452-82 was used in Flow Cytometry to establish intracellular O-linked β-N-acetylglucosamine (O-GlcNAc) modification as a posttranslational switch that dictates the differentiation fate and niche function of early BM stromal cells (BMSCs).

Thu Mar 02 00:00:00 EST 2023

Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.

JCI insight

Zhong L,Yao L,Holdreith N,Yu W,Gui T,Miao Z,Elkaim Y,Li M,Gong Y,Pacifici M,Maity A,Busch TM,Joeng KS,Cengel K,Seale P,Tong W,Qin L

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Fri Apr 08 00:00:00 EDT 2022

Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma.

Cell death & disease

Sun Q,Wang Y,Ji H,Sun X,Xie S,Chen L,Li S,Zeng W,Chen R,Tang Q,Zuo J,Hou L,Hosaka K,Lu Y,Liu Y,Ye Y,Yang Y

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Fri Aug 19 00:00:00 EDT 2022

Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis.

Science advances

Ma L,Tang Q,Gao X,Lee J,Lei R,Suzuki M,Zheng D,Ito K,Frenette PS,Dawlaty MM

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Sphk2 deletion is involved in structural abnormalities and Th17 response but does not aggravate colon inflammation induced by sub-chronic stress.

Scientific reports

Martín-Hernández D,Gutiérrez IL,González-Prieto M,MacDowell KS,Robledo-Montaña J,Tendilla-Beltrán H,Calleja-Rodríguez N,Bris ÁG,Ulecia-Morón C,Moreno B,Caso JR,García-Bueno B,Rodrigues-Mascarenhas S,Marín-Jiménez I,Leza JC,Menchén L

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2022

P140 Peptide Leads to Clearance of Autoreactive Lymphocytes and Normalizes Immune Response in Lupus-Prone Mice.

Frontiers in immunology

Schall N,Talamini L,Wilhelm M,Jouvin-Marche E,Muller S

67-0452-82 was used in Flow cytometry/Cell sorting to suggest that P140 belongs to a new family of non-immunosuppressive immunoregulators that do not correct T and B cell abnormalities but rather contribute to the clearance of deleterious T and B cells.

Tue Jun 21 00:00:00 EDT 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Monocyte-derived dendritic cells link localized secretory IgA deficiency to adaptive immune activation in COPD.

Mucosal immunology

Richmond BW,Mansouri S,Serezani A,Novitskiy S,Blackburn JB,Du RH,Fuseini H,Gutor S,Han W,Schaff J,Vasiukov G,Xin MK,Newcomb DC,Jin L,Blackwell TS,Polosukhin VV

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Mon Mar 01 00:00:00 EST 2021

A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system.

Computational and structural biotechnology journal

Shen Y,Jiang L,Iyer VS,Raposo B,Dubnovitsky A,Boddul SV,Kasza Z,Wermeling F

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Wed Nov 10 00:00:00 EST 2021

Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation.

Disease models & mechanisms

Dobosz E,Lorenz G,Ribeiro A,Würf V,Wadowska M,Kotlinowski J,Schmaderer C,Potempa J,Fu M,Koziel J,Lech M

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Thu Mar 18 00:00:00 EDT 2021

Defining the Mechanistic Correlates of Protection Conferred by Whole-Cell Vaccination against Pseudomonas aeruginosa Acute Murine Pneumonia.

Infection and immunity

Sen-Kilic E,Blackwood CB,Huckaby AB,Horspool AM,Weaver KL,Malkowski AC,Witt WT,Bevere JR,Damron FH,Barbier M

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2021

Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo.

Stem cell research

Sudo K,Yamazaki S,Wilkinson AC,Nakauchi H,Nakamura Y

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Role of CXCL5 in Regulating Chemotaxis of Innate and Adaptive Leukocytes in Infected Lungs Upon Pulmonary Influenza Infection.

Frontiers in immunology

Guo L,Li N,Yang Z,Li H,Zheng H,Yang J,Chen Y,Zhao X,Mei J,Shi H,Worthen GS,Liu L

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Tue Feb 08 00:00:00 EST 2022

Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia.

Cell death & disease

Zaccagnini G,Greco S,Longo M,Maimone B,Voellenkle C,Fuschi P,Carrara M,Creo P,Maselli D,Tirone M,Mazzone M,Gaetano C,Spinetti G,Martelli F

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2021

Ibrutinib does not prevent kidney fibrosis following acute and chronic injury.

Scientific reports

Belliere J,Casemayou A,Colliou E,El Hachem H,Kounde C,Piedrafita A,Feuillet G,Schanstra JP,Faguer S

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Mon Jun 07 00:00:00 EDT 2021

High-throughput analysis of lung immune cells in a combined murine model of agriculture dust-triggered airway inflammation with rheumatoid arthritis.

PloS one

Gaurav R,Mikuls TR,Thiele GM,Nelson AJ,Niu M,Guda C,Eudy JD,Barry AE,Wyatt TA,Romberger DJ,Duryee MJ,England BR,Poole JA

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Thu Jul 08 00:00:00 EDT 2021

Proteostasis in dendritic cells is controlled by the PERK signaling axis independently of ATF4.

Life science alliance

Mendes A,Gigan JP,Rodriguez Rodrigues C,Choteau SA,Sanseau D,Barros D,Almeida C,Camosseto V,Chasson L,Paton AW,Paton JC,Argüello RJ,Lennon-Duménil AM,Gatti E,Pierre P

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Mon Feb 01 00:00:00 EST 2021

Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.

eLife

Hill DL,Whyte CE,Innocentin S,Lee JL,Dooley J,Wang J,James EA,Lee JC,Kwok WW,Zand MS,Liston A,Carr EJ,Linterman MA

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

Metabolic preconditioning in CD4+ T cells restores inducible immune tolerance in lupus-prone mice.

JCI insight

Wilson CS,Stocks BT,Hoopes EM,Rhoads JP,McNew KL,Major AS,Moore DJ

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Fri Oct 08 00:00:00 EDT 2021

The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation.

Nature communications

Shang M,Yang H,Yang R,Chen T,Fu Y,Li Y,Fang X,Zhang K,Zhang J,Li H,Cao X,Gu J,Xiao J,Zhang Q,Liu X,Yu Q,Wang T

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Mon Mar 29 00:00:00 EDT 2021

Type 2 diabetic mice enter a state of spontaneous hibernation-like suspended animation following accumulation of uric acid.

The Journal of biological chemistry

Zhao Y,Cheng R,Zhao Y,Ge W,Yang Y,Ding Z,Xu X,Wang Z,Wu Z,Zhang J

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Targeting Ovarian Carcinoma with TSP-1:CD47 Antagonist TAX2 Activates Anti-Tumor Immunity.

Cancers

Jeanne A,Sarazin T,Charlé M,Moali C,Fichel C,Boulagnon-Rombi C,Callewaert M,Andry MC,Diesis E,Delolme F,Rioult D,Dedieu S

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Thu Oct 07 00:00:00 EDT 2021

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

Wilfahrt D,Philips RL,Lama J,Kizerwetter M,Shapiro MJ,McCue SA,Kennedy MM,Rajcula MJ,Zeng H,Shapiro VS

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Protein Assays and Analysis

Thu Dec 02 00:00:00 EST 2021

Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants.

Science advances

Zhang YN,Paynter J,Sou C,Fourfouris T,Wang Y,Abraham C,Ngo T,Zhang Y,He L,Zhu J

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry (Frozen)

Fri Oct 22 00:00:00 EDT 2021

SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation.

Immunity

Lederer K,Castaño D,Gómez Atria D,Oguin TH,Wang S,Manzoni TB,Muramatsu H,Hogan MJ,Amanat F,Cherubin P,Lundgreen KA,Tam YK,Fan SHY,Eisenlohr LC,Maillard I,Weissman D,Bates P,Krammer F,Sempowski GD,Pardi N,Locci M

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Synergistic factors control kinase-phosphatase organization in B-cells engaged with supported bilayers.

Molecular biology of the cell

Núñez MF,Wisser K,Veatch SL

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunocytochemistry

Thu Mar 19 00:00:00 EDT 2020

Pituitary Adenylate Cyclase-Activating Polypeptide Alleviates Intestinal, Extra-Intestinal and Systemic Inflammatory Responses during Acute Campylobacter jejuni-induced Enterocolitis in Mice.

Pathogens (Basel, Switzerland)

Heimesaat MM,Mousavi S,Kløve S,Genger C,Weschka D,Tamas A,Reglodi D,Bereswill S

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry (Paraffin)

Wed Sep 30 00:00:00 EDT 2020

Pituitary Adenylate Cyclase-Activating Polypeptide Alleviates Intestinal, Extra-Intestinal and Systemic Inflammatory Responses during Acute Campylobacter jejuni-induced Enterocolitis in Mice.

Pathogens (Basel, Switzerland)

Heimesaat MM,Mousavi S,Kløve S,Genger C,Weschka D,Tamas A,Reglodi D,Bereswill S

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry (Paraffin)

Wed Sep 30 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota.

Pathogens (Basel, Switzerland)

Kløve S,Genger C,Mousavi S,Weschka D,Bereswill S,Heimesaat MM

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Mon May 18 00:00:00 EDT 2020

Ischemia Reperfusion Injury Triggers CXCL13 Release and B-Cell Recruitment After Allogenic Kidney Transplantation.

Frontiers in immunology

Kreimann K,Jang MS,Rong S,Greite R,von Vietinghoff S,Schmitt R,Bräsen JH,Schiffer L,Gerstenberg J,Vijayan V,Dittrich-Breiholz O,Wang L,Karsten CM,Gwinner W,Haller H,Immenschuh S,Gueler F

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Mon Apr 12 00:00:00 EDT 2021

Overcoming Immunological Challenges to Helper-Dependent Adenoviral Vector-Mediated Long-Term CFTR Expression in Mouse Airways.

Genes

Cao H,Duan R,Hu J

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Mon May 18 00:00:00 EDT 2020

Myeloid Cell CK2 Regulates Inflammation and Resistance to Bacterial Infection.

Frontiers in immunology

Larson SR,Bortell N,Illies A,Crisler WJ,Matsuda JL,Lenz LL

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity.

Frontiers in immunology

Myers DR,Abram CL,Wildes D,Belwafa A,Welsh AMN,Schulze CJ,Choy TJ,Nguyen T,Omaque N,Hu Y,Singh M,Hansen R,Goldsmith MA,Quintana E,Smith JAM,Lowell CA

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Mon Jun 14 00:00:00 EDT 2021

Fucoxanthin Ameliorates Atopic Dermatitis Symptoms by Regulating Keratinocytes and Regulatory Innate Lymphoid Cells.

International journal of molecular sciences

Natsume C,Aoki N,Aoyama T,Senda K,Matsui M,Ikegami A,Tanaka K,Azuma YT,Fujita T

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Sun Mar 22 00:00:00 EDT 2020

The Host-Specific Intestinal Microbiota Composition Impacts Campylobacter coli Infection in a Clinical Mouse Model of Campylobacteriosis.

Pathogens (Basel, Switzerland)

Heimesaat MM,Genger C,Klove S,Weschka D,Mousavi S,Bereswill S

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Tue Sep 29 00:00:00 EDT 2020

Cep55 overexpression promotes genomic instability and tumorigenesis in mice.

Communications biology

Sinha D,Nag P,Nanayakkara D,Duijf PHG,Burgess A,Raninga P,Smits VAJ,Bain AL,Subramanian G,Wall M,Finnie JW,Kalimutho M,Khanna KK

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Wed Oct 21 00:00:00 EDT 2020

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model.

International journal of molecular sciences

Aung KT,Akiyama K,Kunitomo M,Mun AY,Tosa I,Nguyen HTT,Zhang J,Kohno T,Ono M,Hara ES,Kuboki T

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunocytochemistry

Fri Oct 30 00:00:00 EDT 2020

Requirements for the differentiation of innate T-bet^high memory-phenotype CD4⁺ T lymphocytes under steady state.

Nature communications

Kawabe T,Yi J,Kawajiri A,Hilligan K,Fang D,Ishii N,Yamane H,Zhu J,Jankovic D,Kim KS,Trinchieri G,Sher A

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Immunohistochemistry

Mon Jul 06 00:00:00 EDT 2020

Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.

Nature communications

Ramakrishna C,Kujawski M,Chu H,Li L,Mazmanian SK,Cantin EM

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Tue May 14 00:00:00 EDT 2019

The Hematopoietic Oxidase NOX2 Regulates Self-Renewal of Leukemic Stem Cells.

Cell reports

Adane B,Ye H,Khan N,Pei S,Minhajuddin M,Stevens BM,Jones CL,D'Alessandro A,Reisz JA,Zaberezhnyy V,Gasparetto M,Ho TC,Kelly KK,Myers JR,Ashton JM,Siegenthaler J,Kume T,Campbell EL,Pollyea DA,Becker MW,Jordan CT

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Tue Apr 02 00:00:00 EDT 2019

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using CD45R (B220) monoclonal antibody (Product # 67-0452-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

View more View less

Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; loc; Ly-5; LY5; Lyt-4; PTPRC; T200

View more View less

UniProt ID: (Human) P08575, (Mouse) P06800

View more View less

Entrez Gene ID: (Human) 5788, (Mouse) 19264

View more View less

Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding phosphoprotein phosphatase activity heparin binding hydrolase activity phosphatase activity heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation activation of MAPK activity positive regulation of T cell mediated cytotoxicity positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin leukocyte cell-cell adhesion response to gamma radiation B cell differentiation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion T cell proliferation positive regulation of MAPK cascade positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes negative regulation of peptidyl-tyrosine phosphorylation regulation of B cell receptor signaling pathway regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD45R (B220) Monoclonal Antibody (RA3-6B2), Super Bright™ 702, eBioscience™

CD45R (B220) Antibody (67-0452-82) in Flow

Product Details

67-0452-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!