Invitrogen

CD45R (B220) Monoclonal Antibody (RA3-6B2), PE-Cyanine5.5, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

70 Published Figures

63 References

View all (105) B220 (CD45R) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD45R (B220) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 72

CD45R (B220) Antibody (35-0452-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine5-5 (Product # 35-4321-82) (left) or 0.06 µg of Anti-Human/Mouse CD45R (B220) PE-Cyanine5-5 (right). Total viable cells were used for analysis.

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Product Details

35-0452-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 62 publications 62 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RA3-6B2

Conjugate

PE-Cyanine5.5 PE-Cyanine5.5 PE-Cyanine5.5

Excitation/Emission Max

564/692 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469721

Product Specific Information

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 695 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Platelet factors attenuate inflammation and rescue cognition in ageing.

Nature

Schroer AB,Ventura PB,Sucharov J,Misra R,Chui MKK,Bieri G,Horowitz AM,Smith LK,Encabo K,Tenggara I,Couthouis J,Gross JD,Chan JM,Luke A,Villeda SA

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.

JCI insight

Zhong L,Yao L,Holdreith N,Yu W,Gui T,Miao Z,Elkaim Y,Li M,Gong Y,Pacifici M,Maity A,Busch TM,Joeng KS,Cengel K,Seale P,Tong W,Qin L

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Apr 08 00:00:00 EDT 2022

Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma.

Cell death & disease

Sun Q,Wang Y,Ji H,Sun X,Xie S,Chen L,Li S,Zeng W,Chen R,Tang Q,Zuo J,Hou L,Hosaka K,Lu Y,Liu Y,Ye Y,Yang Y

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Aug 19 00:00:00 EDT 2022

Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis.

Science advances

Ma L,Tang Q,Gao X,Lee J,Lei R,Suzuki M,Zheng D,Ito K,Frenette PS,Dawlaty MM

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Sphk2 deletion is involved in structural abnormalities and Th17 response but does not aggravate colon inflammation induced by sub-chronic stress.

Scientific reports

Martín-Hernández D,Gutiérrez IL,González-Prieto M,MacDowell KS,Robledo-Montaña J,Tendilla-Beltrán H,Calleja-Rodríguez N,Bris ÁG,Ulecia-Morón C,Moreno B,Caso JR,García-Bueno B,Rodrigues-Mascarenhas S,Marín-Jiménez I,Leza JC,Menchén L

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system.

Computational and structural biotechnology journal

Shen Y,Jiang L,Iyer VS,Raposo B,Dubnovitsky A,Boddul SV,Kasza Z,Wermeling F

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Wed Nov 10 00:00:00 EST 2021

Defining the Mechanistic Correlates of Protection Conferred by Whole-Cell Vaccination against Pseudomonas aeruginosa Acute Murine Pneumonia.

Infection and immunity

Sen-Kilic E,Blackwood CB,Huckaby AB,Horspool AM,Weaver KL,Malkowski AC,Witt WT,Bevere JR,Damron FH,Barbier M

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2021

Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo.

Stem cell research

Sudo K,Yamazaki S,Wilkinson AC,Nakauchi H,Nakamura Y

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.

eLife

Hill DL,Whyte CE,Innocentin S,Lee JL,Dooley J,Wang J,James EA,Lee JC,Kwok WW,Zand MS,Liston A,Carr EJ,Linterman MA

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

Metabolic preconditioning in CD4+ T cells restores inducible immune tolerance in lupus-prone mice.

JCI insight

Wilson CS,Stocks BT,Hoopes EM,Rhoads JP,McNew KL,Major AS,Moore DJ

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Oct 08 00:00:00 EDT 2021

The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation.

Nature communications

Shang M,Yang H,Yang R,Chen T,Fu Y,Li Y,Fang X,Zhang K,Zhang J,Li H,Cao X,Gu J,Xiao J,Zhang Q,Liu X,Yu Q,Wang T

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Mon Mar 29 00:00:00 EDT 2021

Type 2 diabetic mice enter a state of spontaneous hibernation-like suspended animation following accumulation of uric acid.

The Journal of biological chemistry

Zhao Y,Cheng R,Zhao Y,Ge W,Yang Y,Ding Z,Xu X,Wang Z,Wu Z,Zhang J

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation.

Immunity

Lederer K,Castaño D,Gómez Atria D,Oguin TH,Wang S,Manzoni TB,Muramatsu H,Hogan MJ,Amanat F,Cherubin P,Lundgreen KA,Tam YK,Fan SHY,Eisenlohr LC,Maillard I,Weissman D,Bates P,Krammer F,Sempowski GD,Pardi N,Locci M

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Myeloid Cell CK2 Regulates Inflammation and Resistance to Bacterial Infection.

Frontiers in immunology

Larson SR,Bortell N,Illies A,Crisler WJ,Matsuda JL,Lenz LL

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity.

Frontiers in immunology

Myers DR,Abram CL,Wildes D,Belwafa A,Welsh AMN,Schulze CJ,Choy TJ,Nguyen T,Omaque N,Hu Y,Singh M,Hansen R,Goldsmith MA,Quintana E,Smith JAM,Lowell CA

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Mon Jun 14 00:00:00 EDT 2021

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

Use of Busulfan to Condition Mice for Bone Marrow Transplantation.

STAR protocols

Montecino-Rodriguez E,Dorshkind K

35-0452 was used in Flow cytometry/Cell sorting to describe a protocol for the use of busulfan to condition mice for bone marrow transplantation and several of the variables to consider for effective implementation.

Fri Dec 18 00:00:00 EST 2020

Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells.

Frontiers in immunology

Xu J,Pei S,Wang Y,Liu J,Qian Y,Huang M,Zhang Y,Xiao Y

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Wed Oct 14 00:00:00 EDT 2020

Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.

Nature communications

Ramakrishna C,Kujawski M,Chu H,Li L,Mazmanian SK,Cantin EM

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue May 14 00:00:00 EDT 2019

The Hematopoietic Oxidase NOX2 Regulates Self-Renewal of Leukemic Stem Cells.

Cell reports

Adane B,Ye H,Khan N,Pei S,Minhajuddin M,Stevens BM,Jones CL,D'Alessandro A,Reisz JA,Zaberezhnyy V,Gasparetto M,Ho TC,Kelly KK,Myers JR,Ashton JM,Siegenthaler J,Kume T,Campbell EL,Pollyea DA,Becker MW,Jordan CT

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Apr 02 00:00:00 EDT 2019

Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.

Nature medicine

Maryanovich M,Zahalka AH,Pierce H,Pinho S,Nakahara F,Asada N,Wei Q,Wang X,Ciero P,Xu J,Leftin A,Frenette PS

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

Proapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner.

Frontiers in immunology

Liu R,King A,Bouillet P,Tarlinton DM,Strasser A,Heierhorst J

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Host Tumor Suppressor p18^INK4c Functions as a Potent Cell-Intrinsic Inhibitor of Murine Gammaherpesvirus 68 Reactivation and Pathogenesis.

Journal of virology

Niemeyer BF,Oko LM,Medina EM,Oldenburg DG,White DW,Cool CD,Clambey ET,van Dyk LF

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Thu Mar 15 00:00:00 EDT 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43.

Experimental and therapeutic medicine

Li Z,Zhang S,Cao L,Li W,Ye YC,Shi ZX,Wang ZR,Sun LX,Wang JW,Jia LT,Wang W

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice.

Frontiers in immunology

Perez-Chacon G,Adrados M,Vallejo-Cremades MT,Lefebvre S,Reed JC,Zapata JM

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Oct 25 00:00:00 EDT 2019

ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.

Frontiers in immunology

Glal D,Sudhakar JN,Lu HH,Liu MC,Chiang HY,Liu YC,Cheng CF,Shui JW

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Thu Sep 26 00:00:00 EDT 2019

Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Nature cell biology

Asada N,Kunisaki Y,Pierce H,Wang Z,Fernandez NF,Birbrair A,Ma'ayan A,Frenette PS

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma.

The Journal of experimental medicine

Takahashi T,Asano Y,Sugawara K,Yamashita T,Nakamura K,Saigusa R,Ichimura Y,Toyama T,Taniguchi T,Akamata K,Noda S,Yoshizaki A,Tsuruta D,Trojanowska M,Sato S

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Mon Apr 03 00:00:00 EDT 2017

EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.

Nature communications

Béguelin W,Rivas MA,Calvo Fernández MT,Teater M,Purwada A,Redmond D,Shen H,Challman MF,Elemento O,Singh A,Melnick AM

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2017

DNA-binding of the Tet-transactivator curtails antigen-induced lymphocyte activation in mice.

Nature communications

Ottina E,Peperzak V,Schoeler K,Carrington E,Sgonc R,Pellegrini M,Preston S,Herold MJ,Strasser A,Villunger A

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Wed Oct 18 00:00:00 EDT 2017

The Interleukin (IL)-1R1 pathway is a critical negative regulator of PyMT-mediated mammary tumorigenesis and pulmonary metastasis.

Oncoimmunology

Dagenais M,Dupaul-Chicoine J,Douglas T,Champagne C,Morizot A,Saleh M

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.

JCI insight

Azzam KM,Madenspacher JH,Cain DW,Lai L,Gowdy KM,Rai P,Janardhan K,Clayton N,Cunningham W,Jensen H,Patel PS,Kearney JF,Taylor GA,Fessler MB

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis.

PloS one

Komegae EN,Souza TA,Grund LZ,Lima C,Lopes-Ferreira M

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Aging-associated inflammation promotes selection for adaptive oncogenic events in B cell progenitors.

The Journal of clinical investigation

Henry CJ,Casás-Selves M,Kim J,Zaberezhnyy V,Aghili L,Daniel AE,Jimenez L,Azam T,McNamee EN,Clambey ET,Klawitter J,Serkova NJ,Tan AC,Dinarello CA,DeGregori J

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Overexpression of Bovine FcRn in Mice Enhances T-Dependent Immune Responses by Amplifying T Helper Cell Frequency and Germinal Center Enlargement in the Spleen.

Frontiers in immunology

Schneider Z,Jani PK,Szikora B,Végh A,Kövesdi D,Iliás A,Cervenak J,Balogh P,Kurucz I,Kacskovics I

35-0452 was used in Flow cytometry/Cell sorting to demonstrate that Tg mice that overexpress bFcRn developed efficient immune response against very low dose of antigen.

Mon Aug 10 00:00:00 EDT 2015

Endothelial Gata5 transcription factor regulates blood pressure.

Nature communications

Messaoudi S,He Y,Gutsol A,Wight A,Hébert RL,Vilmundarson RO,Makrigiannis AP,Chalmers J,Hamet P,Tremblay J,McPherson R,Stewart AFR,Touyz RM,Nemer M

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Mon Nov 30 00:00:00 EST 2015

Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.

Nature communications

Singh NK,Kotla S,Dyukova E,Traylor JG,Orr AW,Chernoff J,Marion TN,Rao GN

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Wed Jun 24 00:00:00 EDT 2015

Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response.

Journal of immunology (Baltimore, Md. : 1950)

Park KS,Bayles I,Szlachta-McGinn A,Paul J,Boiko J,Santos P,Liu J,Wang Z,Borghesi L,Milcarek C

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Sat Nov 01 00:00:00 EDT 2014

IκBε is a key regulator of B cell expansion by providing negative feedback on cRel and RelA in a stimulus-specific manner.

Journal of immunology (Baltimore, Md. : 1950)

Alves BN,Tsui R,Almaden J,Shokhirev MN,Davis-Turak J,Fujimoto J,Birnbaum H,Ponomarenko J,Hoffmann A

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Siglec-G deficiency leads to more severe collagen-induced arthritis and earlier onset of lupus-like symptoms in MRL/lpr mice.

Journal of immunology (Baltimore, Md. : 1950)

Bökers S,Urbat A,Daniel C,Amann K,Smith KG,Espéli M,Nitschke L

35-0452 was used in Flow cytometry/Cell sorting to study how loss of the inhibitory receptor Siglec-G led to a moderate exacerbation of disease severity and early onset in both collagen-induced arthritis and spontaneous lupus nephritis.

Tue Apr 01 00:00:00 EDT 2014

Rhof promotes murine marginal zone B cell development.

Nagoya journal of medical science

Kishimoto M,Matsuda T,Yanase S,Katsumi A,Suzuki N,Ikejiri M,Takagi A,Ikawa M,Kojima T,Kunishima S,Kiyoi H,Naoe T,Matsushita T,Maruyama M

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-κB signalling.

PloS one

Kasama Y,Mizukami T,Kusunoki H,Peveling-Oberhag J,Nishito Y,Ozawa M,Kohara M,Mizuochi T,Tsukiyama-Kohara K

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Mon Dec 22 00:00:00 EST 2014

Reciprocal effects of rab7 deletion in activated and neglected T cells.

Autophagy

Roy SG,Stevens MW,So L,Edinger AL

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.

PLoS genetics

Gómez-Herreros F,Romero-Granados R,Zeng Z,Alvarez-Quilón A,Quintero C,Ju L,Umans L,Vermeire L,Huylebroeck D,Caldecott KW,Cortés-Ledesma F

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase.

Journal of immunology (Baltimore, Md. : 1950)

Hasham MG,Snow KJ,Donghia NM,Branca JA,Lessard MD,Stavnezer J,Shopland LS,Mills KD

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

PAD4-mediated neutrophil extracellular trap formation is not required for immunity against influenza infection.

PloS one

Hemmers S,Teijaro JR,Arandjelovic S,Mowen KA

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2011

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

p27 deficiency cooperates with Bcl-2 but not Bax to promote T-cell lymphoma.

PloS one

Cheng N,van de Wetering CI,Knudson CM

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Wed Apr 02 00:00:00 EDT 2008

Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing.

Molecular and cellular biology

Bai L,Chen Y,He Y,Dai X,Lin X,Wen R,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2007

Essential role of phospholipase C gamma 2 in early B-cell development and Myc-mediated lymphomagenesis.

Molecular and cellular biology

Wen R,Chen Y,Bai L,Fu G,Schuman J,Dai X,Zeng H,Yang C,Stephan RP,Cleveland JL,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2006

A role for brain-derived neurotrophic factor in B cell development.

Journal of neuroimmunology

Schuhmann B,Dietrich A,Sel S,Hahn C,Klingenspor M,Lommatzsch M,Gudermann T,Braun A,Renz H,Nockher WA

Wed Jun 01 00:00:00 EDT 2005

Impaired immune responses and B-cell proliferation in mice lacking the Id3 gene.

Molecular and cellular biology

Pan L,Sato S,Frederick JP,Sun XH,Zhuang Y

Published figure using CD45R (B220) monoclonal antibody (Product # 35-0452-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; loc; Ly-5; LY5; Lyt-4; PTPRC; T200

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UniProt ID: (Human) P08575, (Mouse) P06800

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Entrez Gene ID: (Human) 5788, (Mouse) 19264

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding phosphoprotein phosphatase activity heparin binding hydrolase activity phosphatase activity heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation activation of MAPK activity positive regulation of T cell mediated cytotoxicity positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin leukocyte cell-cell adhesion response to gamma radiation B cell differentiation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion T cell proliferation positive regulation of MAPK cascade positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes negative regulation of peptidyl-tyrosine phosphorylation regulation of B cell receptor signaling pathway regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway

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CD45R (B220) Monoclonal Antibody (RA3-6B2), PE-Cyanine5.5, eBioscience™

CD45R (B220) Antibody (35-0452-82) in Flow

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35-0452-82

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