Invitrogen

Ly-6C Monoclonal Antibody (HK1.4), PE-Cyanine7, eBioscience™

FIGURE: 1 / 70

Ly-6C Antibody (25-5932-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD8a APC (Product # 17-0081-82) and 0.06 µg of Anti-Mouse Ly-6C PE-Cyanine7. Cells in the lymphocyte gate were used for analysis.

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Product Details

25-5932-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 80 publications 80 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2c, kappa

Class

Monoclonal

Type

Antibody

Clone

HK1.4

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573503

Product Specific Information

Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells.

In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release.

Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

TGFβ1⁺CCR5⁺ neutrophil subset increases in bone marrow and causes age-related osteoporosis in male mice.

Nature communications

Li J,Yao Z,Liu X,Duan R,Yi X,Ayoub A,Sanders JO,Mesfin A,Xing L,Boyce BF

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Jan 11 00:00:00 EST 2023

Sympathetic Nerves Coordinate Corneal Epithelial Wound Healing by Controlling the Mobilization of Ly6Chi Monocytes From the Spleen to the Injured Cornea.

Investigative ophthalmology & visual science

He S,Liu J,Xue Y,Fu T,Li Z

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Intranasal Delivery of Endothelial Cell-Derived Extracellular Vesicles with Supramolecular Gel Attenuates Myocardial Ischemia-Reperfusion Injury.

International journal of nanomedicine

Wang J,Tan Y,Dai Y,Hu K,Tan X,Jiang S,Li G,Zhang X,Kang L,Wang X,Xu B

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2023

Macrophagic AMPKα1 orchestrates regenerative inflammation induced by glucocorticoids.

EMBO reports

Caratti G,Desgeorges T,Juban G,Stifel U,Fessard A,Koenen M,Caratti B,Théret M,Skurk C,Chazaud B,Tuckermann JP,Mounier R

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Mon Feb 06 00:00:00 EST 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.

PLoS biology

Tóth K,Lénárt N,Berki P,Fekete R,Szabadits E,Pósfai B,Cserép C,Alatshan A,Benkő S,Kiss D,Hübner CA,Gulyás A,Kaila K,Környei Z,Dénes Á

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

Gene-dosage effect of Pfkfb3 on monocyte/macrophage biology in atherosclerosis.

British journal of pharmacology

Guo S,Li A,Fu X,Li Z,Cao K,Song M,Huang S,Li Z,Yan J,Wang L,Dai X,Feng D,Wang Y,He J,Huo Y,Xu Y

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Mineralocorticoid receptor antagonists and glucocorticoids differentially affect skeletal muscle inflammation and pathology in muscular dystrophy.

JCI insight

Howard ZM,Gomatam CK,Rabolli CP,Lowe J,Piepho AB,Bansal SS,Accornero F,Rafael-Fortney JA

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Mon Oct 10 00:00:00 EDT 2022

Therapeutic CFTR Correction Normalizes Systemic and Lung-Specific S1P Level Alterations Associated with Heart Failure.

International journal of molecular sciences

Uhl FE,Vanherle L,Matthes F,Meissner A

25-5932-82 was used in Flow cytometry/Cell sorting to suggest an important role for the CFTR-S1P axis in HF-mediated systemic and pulmonary inflammation.

Fri Jan 14 00:00:00 EST 2022

TNF hampers intestinal tissue repair in colitis by restricting IL-22 bioavailability.

Mucosal immunology

Ninnemann J,Winsauer C,Bondareva M,Kühl AA,Lozza L,Durek P,Lissner D,Siegmund B,Kaufmann SHE,Mashreghi MF,Nedospasov SA,Kruglov AA

25-5932-82 was used in Flow cytometry/Cell sorting to show how anti-TNF therapy induces mucosal healing by increasing IL-22 availability and implicates new therapeutic opportunities for IBD.

Fri Apr 01 00:00:00 EDT 2022

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Pancreatic Cancer Chemotherapy Is Potentiated by Induction of Tertiary Lymphoid Structures in Mice.

Cellular and molecular gastroenterology and hepatology

Delvecchio FR,Fincham REA,Spear S,Clear A,Roy-Luzarraga M,Balkwill FR,Gribben JG,Bombardieri M,Hodivala-Dilke K,Capasso M,Kocher HM

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Mon Mar 28 00:00:00 EDT 2022

Disrupted Iron Metabolism and Mortality during Co-infection with Malaria and an Intestinal Gram-Negative Extracellular Pathogen.

Cell reports

Dos Santos LI,Torres TA,Diniz SQ,Gonçalves R,Caballero-Flores G,Núñez G,Gazzinelli RT,Maloy KJ,Ribeiro do V Antonelli L

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Myeloid DJ-1 deficiency protects acetaminophen-induced acute liver injury through decreasing inflammatory response.

Aging

Wang B,Li J,Jiao J,Xu M,Luo Y,Wang F,Xia Q,Gao Y,Feng Y,Kong X,Sun X

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Jul 21 00:00:00 EDT 2021

NOX4 regulates macrophage apoptosis resistance to induce fibrotic progression.

The Journal of biological chemistry

Larson-Casey JL,Gu L,Kang J,Dhyani A,Carter AB

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

Abraxane-induced bone marrow CD11b⁺ myeloid cell depletion in tumor-bearing mice is visualized by μPET-CT with ⁶⁴Cu-labeled anti-CD11b and prevented by anti-CSF-1.

Theranostics

Cao Q,Huang Q,Wang YA,Li C

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Fri Jul 23 00:00:00 EDT 2021

Inflammatory resolution and vascular barrier restoration after retinal ischemia reperfusion injury.

Journal of neuroinflammation

Abcouwer SF,Shanmugam S,Muthusamy A,Lin CM,Kong D,Hager H,Liu X,Antonetti DA

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

Roles of lung-recruited monocytes and pulmonary Vascular Endothelial Growth Factor (VEGF) in resolving Ventilator-Induced Lung Injury (VILI).

PloS one

Lin CK,Huang TH,Yang CT,Shi CS

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization.

Cell death & disease

Liu L,Wu Y,Wang B,Jiang Y,Lin L,Li X,Yang S

25-5932 was used in Flow cytometry/Cell sorting to provide demonstration of DA-DRD5 signaling in colonic macrophages controlling the development of colitis by regulating M1/M2 macrophage polarization.

Mon May 17 00:00:00 EDT 2021

Intestinal barrier dysfunction plays an integral role in arthritis pathology and can be targeted to ameliorate disease.

Med (New York, N.Y.)

Matei DE,Menon M,Alber DG,Smith AM,Nedjat-Shokouhi B,Fasano A,Magill L,Duhlin A,Bitoun S,Gleizes A,Hacein-Bey-Abina S,Manson JJ,Rosser EC,Klein N,Blair PA,Mauri C

25-5932-82 was used in Flow Cytometry to analyze changes in markers of gut permeability, damage, and inflammation in peripheral blood and serum of rheumatoid arthritis patients, as well as relevant organs in engineered arthritic mice.

Fri Jul 09 00:00:00 EDT 2021

Plaque-associated myeloid cells derive from resident microglia in an Alzheimer's disease model.

The Journal of experimental medicine

Reed-Geaghan EG,Croxford AL,Becher B,Landreth GE

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Mon Apr 06 00:00:00 EDT 2020

Proenkephalin⁺ regulatory T cells expanded by ultraviolet B exposure maintain skin homeostasis with a healing function.

Proceedings of the National Academy of Sciences of the United States of America

Shime H,Odanaka M,Tsuiji M,Matoba T,Imai M,Yasumizu Y,Uraki R,Minohara K,Watanabe M,Bonito AJ,Fukuyama H,Ohkura N,Sakaguchi S,Morita A,Yamazaki S

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Tue Aug 25 00:00:00 EDT 2020

IgE Contributes to Atherosclerosis and Obesity by Affecting Macrophage Polarization, Macrophage Protein Network, and Foam Cell Formation.

Arteriosclerosis, thrombosis, and vascular biology

Zhang X,Li J,Luo S,Wang M,Huang Q,Deng Z,de Febbo C,Daoui A,Liew PX,Sukhova GK,Metso J,Jauhiainen M,Shi GP,Guo J

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Modulation of the functions of myeloid-derived suppressor cells : a new strategy of hydrogen sulfide anti-cancer effects.

British journal of pharmacology

De Cicco P,Ercolano G,Rubino V,Terrazzano G,Ruggiero G,Cirino G,Ianaro A

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Temporospatial distribution and transcriptional profile of retinal microglia in the oxygen-induced retinopathy mouse model.

Glia

Boeck M,Thien A,Wolf J,Hagemeyer N,Laich Y,Yusuf D,Backofen R,Zhang P,Boneva S,Stahl A,Hilgendorf I,Agostini H,Prinz M,Wieghofer P,Schlunck G,Schlecht A,Lange C

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Astrocytes promote a protective immune response to brain Toxoplasma gondii infection via IL-33-ST2 signaling.

PLoS pathogens

Still KM,Batista SJ,O'Brien CA,Oyesola OO,Früh SP,Webb LM,Smirnov I,Kovacs MA,Cowan MN,Hayes NW,Thompson JA,Tait Wojno ED,Harris TH

25-5932 was used in Flow cytometry/Cell sorting to show that IL-33 is expressed by oligodendrocytes and astrocytes during T. gondii infection, is released locally into the cerebrospinal fluid of T. gondii-infected animals, and is required for control of infection.

Thu Oct 01 00:00:00 EDT 2020

Characterization and regulation of osteoclast precursors following chronic Porphyromonas gingivalis infection.

Journal of leukocyte biology

Zhao Y,Li Z,Su L,Ballesteros-Tato A,Katz J,Michalek SM,Feng X,Zhang P

25-5932-82 was used in Flow Cytometry to provide new insights into the characterization and regulation of OCP following a chronic bacterial infection.

Thu Oct 01 00:00:00 EDT 2020

Characterization and regulation of osteoclast precursors following chronic Porphyromonas gingivalis infection.

Journal of leukocyte biology

Zhao Y,Li Z,Su L,Ballesteros-Tato A,Katz J,Michalek SM,Feng X,Zhang P

25-5932-82 was used in Flow Cytometry to provide new insights into the characterization and regulation of OCP following a chronic bacterial infection.

Thu Oct 01 00:00:00 EDT 2020

Antitumor Effect and Immune Response of Nanosecond Pulsed Electric Fields in Pancreatic Cancer.

Frontiers in oncology

Zhao J,Chen S,Zhu L,Zhang L,Liu J,Xu D,Tian G,Jiang T

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Fri Oct 28 00:00:00 EDT 2022

Methylprednisolone alleviates multiple sclerosis by expanding myeloid-derived suppressor cells via glucocorticoid receptor β and S100A8/9 up-regulation.

Journal of cellular and molecular medicine

Wang Z,Zheng G,Li G,Wang M,Ma Z,Li H,Wang XY,Yi H

25-5932 was used in Flow cytometry/Cell sorting to suggest that GCs treatment may help relieve the acute phase of MS by expanding MDSC through up-regulating of GR signalling and S100A8/A9 heterodimers.

Tue Dec 01 00:00:00 EST 2020

Effects of Hypertonic Saline and Hydroxyethyl Starch on Myeloid-Derived Suppressor Cells in Hemorrhagic Shock Mice under Secondary Bacterial Attack.

BioMed research international

Jiang JK,Hong LJ,Lu YQ

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Site-1 protease ablation in the osterix-lineage in mice results in bone marrow neutrophilia and hematopoietic stem cell alterations.

Biology open

Patra D,Kim J,Zhang Q,Tycksen E,Sandell LJ

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Tue Jun 23 00:00:00 EDT 2020

The impact of Clonorchis sinensis infection on immune response in mice with type II collagen-induced arthritis.

BMC immunology

Li X,Yang Y,Qin S,Kong F,Yan C,Cheng W,Pan W,Yu Q,Hua H,Zheng K,Tang R

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Mon Feb 17 00:00:00 EST 2020

The impact of Clonorchis sinensis infection on immune response in mice with type II collagen-induced arthritis.

BMC immunology

Li X,Yang Y,Qin S,Kong F,Yan C,Cheng W,Pan W,Yu Q,Hua H,Zheng K,Tang R

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Mon Feb 17 00:00:00 EST 2020

GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype.

Cells

Wang L,Li Y,Wang X,Wang P,Essandoh K,Cui S,Huang W,Mu X,Liu Z,Wang Y,Peng T,Fan GC

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Fri Jan 03 00:00:00 EST 2020

The Transcription Factor Nfix Requires RhoA-ROCK1 Dependent Phagocytosis to Mediate Macrophage Skewing during Skeletal Muscle Regeneration.

Cells

Saclier M,Lapi M,Bonfanti C,Rossi G,Antonini S,Messina G

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Fri Mar 13 00:00:00 EDT 2020

Extracellular miR-146a-5p Induces Cardiac Innate Immune Response and Cardiomyocyte Dysfunction.

ImmunoHorizons

Shimada BK,Yang Y,Zhu J,Wang S,Suen A,Kronstadt SM,Jeyaram A,Jay SM,Zou L,Chao W

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Mon Sep 21 00:00:00 EDT 2020

Extracellular miR-146a-5p Induces Cardiac Innate Immune Response and Cardiomyocyte Dysfunction.

ImmunoHorizons

Shimada BK,Yang Y,Zhu J,Wang S,Suen A,Kronstadt SM,Jeyaram A,Jay SM,Zou L,Chao W

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Mon Sep 21 00:00:00 EDT 2020

Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation.

Science advances

Ma C,Yang D,Wang B,Wu C,Wu Y,Li S,Liu X,Lassen K,Dai L,Yang S

25-5932 was used in Flow cytometry/Cell sorting to provide the first demonstration of GSDMD's role in controlling colitis and a detailed delineation of the underlying mechanism.

Fri May 01 00:00:00 EDT 2020

Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation.

Science advances

Ma C,Yang D,Wang B,Wu C,Wu Y,Li S,Liu X,Lassen K,Dai L,Yang S

25-5932 was used in Flow cytometry/Cell sorting to provide the first demonstration of GSDMD's role in controlling colitis and a detailed delineation of the underlying mechanism.

Fri May 01 00:00:00 EDT 2020

Resident macrophages acquire innate immune memory in staphylococcal skin infection.

eLife

Feuerstein R,Forde AJ,Lohrmann F,Kolter J,Ramirez NJ,Zimmermann J,Gomez de Agüero M,Henneke P

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Jul 08 00:00:00 EDT 2020

Squalene emulsion-based vaccine adjuvants stimulate CD8 T cell, but not antibody responses, through a RIPK3-dependent pathway.

eLife

Kim EH,Woodruff MC,Grigoryan L,Maier B,Lee SH,Mandal P,Cortese M,Natrajan MS,Ravindran R,Ma H,Merad M,Gitlin AD,Mocarski ES,Jacob J,Pulendran B

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Tue Jun 09 00:00:00 EDT 2020

Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

Nature communications

Sidwell T,Liao Y,Garnham AL,Vasanthakumar A,Gloury R,Blume J,Teh PP,Chisanga D,Thelemann C,de Labastida Rivera F,Engwerda CR,Corcoran L,Kometani K,Kurosaki T,Smyth GK,Shi W,Kallies A

25-5932 was used in Flow cytometry/Cell sorting to show homeostasis of thymically-derived and peripherally-derived Treg cells is maintained through the balancing of TCR signaling induced transcriptional activity by Bach2.

Tue Jan 14 00:00:00 EST 2020

Host deficiency in ephrin-A1 inhibits breast cancer metastasis.

F1000Research

Shiuan E,Inala A,Wang S,Song W,Youngblood V,Chen J,Brantley-Sieders DM

25-5932 was used in Flow cytometry/Cell sorting to study how ephrin-A1 host deficiency affects cancer growth and metastasis in a murine model of breast cancer.

Mon Feb 01 00:00:00 EST 2021

Host Cathelicidin Exacerbates Group B Streptococcus Urinary Tract Infection.

mSphere

Patras KA,Coady A,Babu P,Shing SR,Ha AD,Rooholfada E,Brandt SL,Geriak M,Gallo RL,Nizet V

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Apr 22 00:00:00 EDT 2020

Host Cathelicidin Exacerbates Group B Streptococcus Urinary Tract Infection.

mSphere

Patras KA,Coady A,Babu P,Shing SR,Ha AD,Rooholfada E,Brandt SL,Geriak M,Gallo RL,Nizet V

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Apr 22 00:00:00 EDT 2020

Regulatory T Cells Support Breast Cancer Progression by Opposing IFN-γ-Dependent Functional Reprogramming of Myeloid Cells.

Cell reports

Clark NM,Martinez LM,Murdock S,deLigio JT,Olex AL,Effi C,Dozmorov MG,Bos PD

25-5932 was used in Flow cytometry/Cell sorting to highlight the strong myeloid dependency of breast cancer and provide the basis for the development of therapeutic strategies based on manipulation of the IFN-γ signaling pathway in monocytes.

Tue Dec 08 00:00:00 EST 2020

Increased flux through the mevalonate pathway mediates fibrotic repair without injury.

The Journal of clinical investigation

Larson-Casey JL,Vaid M,Gu L,He C,Cai GQ,Ding Q,Davis D,Berryhill TF,Wilson LS,Barnes S,Neighbors JD,Hohl RJ,Zimmerman KA,Yoder BK,Longhini ALF,Hanumanthu VS,Surolia R,Antony VB,Carter AB

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

ABRO1 promotes NLRP3 inflammasome activation through regulation of NLRP3 deubiquitination.

The EMBO journal

Ren G,Zhang X,Xiao Y,Zhang W,Wang Y,Ma W,Wang X,Song P,Lai L,Chen H,Zhan Y,Zhang J,Yu M,Ge C,Li C,Yin R,Yang X

25-5932 was used in Flow cytometry/Cell sorting to study an ABRO1-mediated regulatory signaling system that controls activation of the NLRP3 inflammasome.

Fri Mar 15 00:00:00 EDT 2019

TCR Affinity Biases Th Cell Differentiation by Regulating CD25, Eef1e1, and Gbp2.

Journal of immunology (Baltimore, Md. : 1950)

Kotov DI,Mitchell JS,Pengo T,Ruedl C,Way SS,Langlois RA,Fife BT,Jenkins MK

25-5932-82 was used in Flow Cytometry to study TCR affinity and influence on regulation of genes in naive T cells that control the differentiation process in cells.

Wed May 01 00:00:00 EDT 2019

The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis.

Cell reports

Hou X,Chen G,Bracamonte-Baran W,Choi HS,Diny NL,Sung J,Hughes D,Won T,Wood MK,Talor MV,Hackam DJ,Klingel K,Davogustto G,Taegtmeyer H,Coppens I,Barin JG,Čiháková D

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Tue Jul 02 00:00:00 EDT 2019

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

25-5932-82 was used in Flow cytometry/Cell sorting to investigate Treg cell function in the progression from non-invasive to invasive breast cancer lesions.

Tue Sep 29 00:00:00 EDT 2020

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

25-5932-82 was used in Flow cytometry/Cell sorting to investigate Treg cell function in the progression from non-invasive to invasive breast cancer lesions.

Tue Sep 29 00:00:00 EDT 2020

Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner.

Cell

Thion MS,Low D,Silvin A,Chen J,Grisel P,Schulte-Schrepping J,Blecher R,Ulas T,Squarzoni P,Hoeffel G,Coulpier F,Siopi E,David FS,Scholz C,Shihui F,Lum J,Amoyo AA,Larbi A,Poidinger M,Buttgereit A,Lledo PM,Greter M,Chan JKY,Amit I,Beyer M,Schultze JL,Schlitzer A,Pettersson S,Ginhoux F,Garel S

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

Advanced Glycation End Products Enhance Murine Monocyte Proliferation in Bone Marrow and Prime Them into an Inflammatory Phenotype through MAPK Signaling.

Journal of diabetes research

Jin X,Liu L,Zhang Y,Xiang Y,Yin G,Lu Y,Shi L,Dong J,Shen C

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

iRhom2-mediated proinflammatory signalling regulates heart repair following myocardial infarction.

JCI insight

Barnette DN,Cahill TJ,Gunadasa-Rohling M,Carr CA,Freeman M,Riley PR

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Thu Feb 08 00:00:00 EST 2018

Differentiated macrophages acquire a pro-inflammatory and cell death-resistant phenotype due to increasing XIAP and p38-mediated inhibition of RipK1.

The Journal of biological chemistry

Rijal D,Ariana A,Wight A,Kim K,Alturki NA,Aamir Z,Ametepe ES,Korneluk RG,Tiedje C,Menon MB,Gaestel M,McComb S,Sad S

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Fri Jul 27 00:00:00 EDT 2018

The Combined Use of Melatonin and an Indoleamine 2,3-Dioxygenase-1 Inhibitor Enhances Vaccine-Induced Protective Cellular Immunity to HPV16-Associated Tumors.

Frontiers in immunology

Moreno ACR,Porchia BFMM,Pagni RL,Souza PDC,Pegoraro R,Rodrigues KB,Barros TB,Aps LRMM,de Araújo EF,Calich VLG,Ferreira LCS

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Thu Sep 19 00:00:00 EDT 2019

Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge.

Nature communications

Yi S,Zhai J,Niu R,Zhu G,Wang M,Liu J,Huang H,Wang Y,Jing X,Kang L,Song W,Shi Y,Tang H

25-5932 was used in Flow cytometry/Cell sorting to suggest that different lung antigen-presenting cells modulate lung cDC1-mediated eosinophil recruitment dynamically.

Mon Sep 24 00:00:00 EDT 2018

Lack of FcRn Impairs Natural Killer Cell Development and Functions in the Tumor Microenvironment.

Frontiers in immunology

Castaneda DC,Dhommée C,Baranek T,Dalloneau E,Lajoie L,Valayer A,Arnoult C,Demattéi MV,Fouquenet D,Parent C,Heuzé-Vourc'h N,Gouilleux-Gruart V

25-5932 was used in Flow cytometry/Cell sorting to elucidate the consequences of neonatal Fc receptor down-regulation in the anti-tumour immune response.

Mon Oct 21 00:00:00 EDT 2019

CCR2⁺ Monocyte-Derived Infiltrating Macrophages Are Required for Adverse Cardiac Remodeling During Pressure Overload.

JACC. Basic to translational science

Patel B,Bansal SS,Ismahil MA,Hamid T,Rokosh G,Mack M,Prabhu SD

Sun Apr 01 00:00:00 EDT 2018

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

25-5932 was used in Flow cytometry/Cell sorting to study whether helminth-induced type 2 immunity functionally affects the T virtual memory cell compartment.

Tue Oct 30 00:00:00 EDT 2018

Non-redundant Functions of IL-6 Produced by Macrophages and Dendritic Cells in Allergic Airway Inflammation.

Frontiers in immunology

Gubernatorova EO,Gorshkova EA,Namakanova OA,Zvartsev RV,Hidalgo J,Drutskaya MS,Tumanov AV,Nedospasov SA

25-5932 was used in Flow cytometry/Cell sorting to indicate that IL-6 plays a pathogenic role in the HDM-induced asthma model and that lung macrophages and dendritic cells are the predominant sources of pathogenic IL-6 but contribute differently to the disease.

Wed Oct 02 00:00:00 EDT 2019

Mir-223 regulates the number and function of myeloid-derived suppressor cells in multiple sclerosis and experimental autoimmune encephalomyelitis.

Acta neuropathologica

Cantoni C,Cignarella F,Ghezzi L,Mikesell B,Bollman B,Berrien-Elliott MM,Ireland AR,Fehniger TA,Wu GF,Piccio L

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

CCR2^- and CCR2⁺ corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion.

Mucosal immunology

Liu J,Xue Y,Dong D,Xiao C,Lin C,Wang H,Song F,Fu T,Wang Z,Chen J,Pan H,Li Y,Cai D,Li Z

25-5932 was used in Flow cytometry/Cell sorting to show how CCR2(-) and CCR2(+) corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion.

Fri Sep 01 00:00:00 EDT 2017

Infiltrating Myeloid Cells Exert Protumorigenic Actions via Neutrophil Elastase.

Molecular cancer research : MCR

Lerman I,Garcia-Hernandez ML,Rangel-Moreno J,Chiriboga L,Pan C,Nastiuk KL,Krolewski JJ,Sen A,Hammes SR

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Cardiac inflammation in genetic dilated cardiomyopathy caused by MYBPC3 mutation.

Journal of molecular and cellular cardiology

Lynch TL,Ismahil MA,Jegga AG,Zilliox MJ,Troidl C,Prabhu SD,Sadayappan S

25-5932 was used in Flow cytometry/Cell sorting to show that a robust proinflammatory response occurs in the hearts of mice with dilated cardiomyopathy caused by a mutation in the cardiac myosin binding protein-C gene.

Sun Jan 01 00:00:00 EST 2017

Activation of the NLRP3 Inflammasome Is Associated with Valosin-Containing Protein Myopathy.

Inflammation

Nalbandian A,Khan AA,Srivastava R,Llewellyn KJ,Tan B,Shukr N,Fazli Y,Kimonis VE,BenMohamed L

25-5932 was used in Flow cytometry/Cell sorting to investigate whether the NLRP3 inflammasome plays a role in valosin-containing protein-associated diseases, which classically affects the muscles, bones, and brain.

Wed Feb 01 00:00:00 EST 2017

Spatiotemporally restricted arenavirus replication induces immune surveillance and type I interferon-dependent tumour regression.

Nature communications

Kalkavan H,Sharma P,Kasper S,Helfrich I,Pandyra AA,Gassa A,Virchow I,Flatz L,Brandenburg T,Namineni S,Heikenwalder M,Höchst B,Knolle PA,Wollmann G,von Laer D,Drexler I,Rathbun J,Cannon PM,Scheu S,Bauer J,Chauhan J,Häussinger D,Willimsky G,Löhning M,Schadendorf D,Brandau S,Schuler M,Lang PA,Lang KS

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Mononuclear Phagocytes Are Dispensable for Cardiac Remodeling in Established Pressure-Overload Heart Failure.

PloS one

Patel B,Ismahil MA,Hamid T,Bansal SS,Prabhu SD

25-5932 was used in Flow cytometry/Cell sorting to assess for alterations in MPs.

Thu Aug 10 00:00:00 EDT 2017

BMP Antagonist Gremlin 2 Limits Inflammation After Myocardial Infarction.

Circulation research

Sanders LN,Schoenhard JA,Saleh MA,Mukherjee A,Ryzhov S,McMaster WG,Nolan K,Gumina RJ,Thompson TB,Magnuson MA,Harrison DG,Hatzopoulos AK

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Fri Jul 22 00:00:00 EDT 2016

Alternatively activated macrophages determine repair of the infarcted adult murine heart.

The Journal of clinical investigation

Shiraishi M,Shintani Y,Shintani Y,Ishida H,Saba R,Yamaguchi A,Adachi H,Yashiro K,Suzuki K

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Tissue damage negatively regulates LPS-induced macrophage necroptosis.

Cell death and differentiation

Li Z,Scott MJ,Fan EK,Li Y,Liu J,Xiao G,Li S,Billiar TR,Wilson MA,Jiang Y,Fan J

25-5932 was used in Flow cytometry/Cell sorting to investigate the mechanism and role of trauma/tissue damage in the regulation of LPS-induced MΦ necroptosis.

Thu Sep 01 00:00:00 EDT 2016

Macrophages transfer antigens to dendritic cells by releasing exosomes containing dead-cell-associated antigens partially through a ceramide-dependent pathway to enhance CD4(+) T-cell responses.

Immunology

Xu Y,Liu Y,Yang C,Kang L,Wang M,Hu J,He H,Song W,Tang H

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2016

Multimodal analysis of ocular inflammation using the endotoxin-induced uveitis mouse model.

Disease models & mechanisms

Chu CJ,Gardner PJ,Copland DA,Liyanage SE,Gonzalez-Cordero A,Kleine Holthaus SM,Luhmann UF,Smith AJ,Ali RR,Dick AD

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

25-5932 was used in Flow cytometry/Cell sorting to suggest persistent viral lytic gene expression during latency causes chronic inflammatory response inducing exhaustion of resident T cells in the ependyma.

Mon Aug 15 00:00:00 EDT 2016

Trypanosoma cruzi Causes Paralyzing Systemic Necrotizing Vasculitis Driven by Pathogen-Specific Type I Immunity in Mice.

Infection and immunity

Roffê E,Marino APMP,Weaver J,Wan W,de Araújo FF,Hoffman V,Santiago HC,Murphy PM

25-5932 was used in Flow cytometry/Cell sorting to investigate T. cruzi infection as a trigger of vasculitis.

Fri Apr 01 00:00:00 EDT 2016

Stromal senescence establishes an immunosuppressive microenvironment that drives tumorigenesis.

Nature communications

Ruhland MK,Loza AJ,Capietto AH,Luo X,Knolhoff BL,Flanagan KC,Belt BA,Alspach E,Leahy K,Luo J,Schaffer A,Edwards JR,Longmore G,Faccio R,DeNardo DG,Stewart SA

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Jun 08 00:00:00 EDT 2016

Ranitidine modifies myeloid cell populations and inhibits breast tumor development and spread in mice.

Oncoimmunology

Vila-Leahey A,Oldford SA,Marignani PA,Wang J,Haidl ID,Marshall JS

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

The impact of ranitidine on monocyte responses in the context of solid tumors.

Oncotarget

Vila-Leahey A,Rogers D,Marshall JS

25-5932 was used in Flow cytometry/Cell sorting to examine the impact of oral ranitidine treatment on multiple murine tumour models.

Tue Mar 08 00:00:00 EST 2016

Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine.

Scientific reports

Ochi T,Feng Y,Kitamoto S,Nagao-Kitamoto H,Kuffa P,Atarashi K,Honda K,Teitelbaum DH,Kamada N

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Wed Jun 15 00:00:00 EDT 2016

Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke.

Nature communications

Szalay G,Martinecz B,Lénárt N,Környei Z,Orsolits B,Judák L,Császár E,Fekete R,West BL,Katona G,Rózsa B,Dénes Á

25-5932 was used in Flow cytometry/Cell sorting to investigate the influence of microglia on neuronal activity and survival in the injured brain in vivo.

Tue May 03 00:00:00 EDT 2016

Obesity-associated NLRC4 inflammasome activation drives breast cancer progression.

Nature communications

Kolb R,Phan L,Borcherding N,Liu Y,Yuan F,Janowski AM,Xie Q,Markan KR,Li W,Potthoff MJ,Fuentes-Mattei E,Ellies LG,Knudson CM,Lee MH,Yeung SJ,Cassel SL,Sutterwala FS,Zhang W

25-5932 was used in Flow cytometry/Cell sorting to provide a causal mechanism by which obesity promotes breast cancer progression and lay out a foundation to block NLRC4 inflammasome activation or IL-1β signalling transduction for the treatment of obese cancer patients.

Thu Oct 06 00:00:00 EDT 2016

Cysteine cathepsin activity suppresses osteoclastogenesis of myeloid-derived suppressor cells in breast cancer.

Oncotarget

Edgington-Mitchell LE,Rautela J,Duivenvoorden HM,Jayatilleke KM,van der Linden WA,Verdoes M,Bogyo M,Parker BS

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2015

Glioma-associated microglia/macrophages display an expression profile different from M1 and M2 polarization and highly express Gpnmb and Spp1.

PloS one

Szulzewsky F,Pelz A,Feng X,Synowitz M,Markovic D,Langmann T,Holtman IR,Wang X,Eggen BJ,Boddeke HW,Hambardzumyan D,Wolf SA,Kettenmann H

Published figure using Ly-6C monoclonal antibody (Product # 25-5932-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2016

Bioinformatics

Protein Aliases: Ly-6C1; Ly6c2; lymphocyte antigen 6 complex, locus C; Lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C

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Gene Aliases: AA682074; AA959465; Ly-6C; Ly-6C1; Ly6c; Ly6c1

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UniProt ID: (Mouse) P0CW02

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Entrez Gene ID: (Mouse) 17067

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Ly-6C Monoclonal Antibody (HK1.4), PE-Cyanine7, eBioscience™

Ly-6C Antibody (25-5932-82) in Flow

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25-5932-82

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