Invitrogen

Ly-6C Monoclonal Antibody (HK1.4), Super Bright™ 436, eBioscience™

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Ly-6C Antibody (62-5932-82) in Flow

Staining of BALB/c splenocytes with CD8a Monoclonal Antibody, FITC (Product # 11-0081-82) and 0.5 µg of Ly-6C Monoclonal Antibody, Super Bright 436. Total viable cells were used for analysis.

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Product Details

62-5932-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1.0 µg/test

View 38 publications 38 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2c, kappa

Class

Monoclonal

Type

Antibody

Clone

HK1.4

Conjugate

Super Bright™ 436 Super Bright™ 436 Super Bright™ 436

Excitation/Emission Max

413/431 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735067

Product Specific Information

Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells.

In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release.

Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

TGFβ1⁺CCR5⁺ neutrophil subset increases in bone marrow and causes age-related osteoporosis in male mice.

Nature communications

Li J,Yao Z,Liu X,Duan R,Yi X,Ayoub A,Sanders JO,Mesfin A,Xing L,Boyce BF

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Wed Jan 11 00:00:00 EST 2023

Sympathetic Nerves Coordinate Corneal Epithelial Wound Healing by Controlling the Mobilization of Ly6Chi Monocytes From the Spleen to the Injured Cornea.

Investigative ophthalmology & visual science

He S,Liu J,Xue Y,Fu T,Li Z

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Intranasal Delivery of Endothelial Cell-Derived Extracellular Vesicles with Supramolecular Gel Attenuates Myocardial Ischemia-Reperfusion Injury.

International journal of nanomedicine

Wang J,Tan Y,Dai Y,Hu K,Tan X,Jiang S,Li G,Zhang X,Kang L,Wang X,Xu B

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2023

Macrophagic AMPKα1 orchestrates regenerative inflammation induced by glucocorticoids.

EMBO reports

Caratti G,Desgeorges T,Juban G,Stifel U,Fessard A,Koenen M,Caratti B,Théret M,Skurk C,Chazaud B,Tuckermann JP,Mounier R

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Mon Feb 06 00:00:00 EST 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.

PLoS biology

Tóth K,Lénárt N,Berki P,Fekete R,Szabadits E,Pósfai B,Cserép C,Alatshan A,Benkő S,Kiss D,Hübner CA,Gulyás A,Kaila K,Környei Z,Dénes Á

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

Gene-dosage effect of Pfkfb3 on monocyte/macrophage biology in atherosclerosis.

British journal of pharmacology

Guo S,Li A,Fu X,Li Z,Cao K,Song M,Huang S,Li Z,Yan J,Wang L,Dai X,Feng D,Wang Y,He J,Huo Y,Xu Y

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Mineralocorticoid receptor antagonists and glucocorticoids differentially affect skeletal muscle inflammation and pathology in muscular dystrophy.

JCI insight

Howard ZM,Gomatam CK,Rabolli CP,Lowe J,Piepho AB,Bansal SS,Accornero F,Rafael-Fortney JA

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Mon Oct 10 00:00:00 EDT 2022

Therapeutic CFTR Correction Normalizes Systemic and Lung-Specific S1P Level Alterations Associated with Heart Failure.

International journal of molecular sciences

Uhl FE,Vanherle L,Matthes F,Meissner A

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Fri Jan 14 00:00:00 EST 2022

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Pancreatic Cancer Chemotherapy Is Potentiated by Induction of Tertiary Lymphoid Structures in Mice.

Cellular and molecular gastroenterology and hepatology

Delvecchio FR,Fincham REA,Spear S,Clear A,Roy-Luzarraga M,Balkwill FR,Gribben JG,Bombardieri M,Hodivala-Dilke K,Capasso M,Kocher HM

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Mon Mar 28 00:00:00 EDT 2022

Disrupted Iron Metabolism and Mortality during Co-infection with Malaria and an Intestinal Gram-Negative Extracellular Pathogen.

Cell reports

Dos Santos LI,Torres TA,Diniz SQ,Gonçalves R,Caballero-Flores G,Núñez G,Gazzinelli RT,Maloy KJ,Ribeiro do V Antonelli L

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Myeloid DJ-1 deficiency protects acetaminophen-induced acute liver injury through decreasing inflammatory response.

Aging

Wang B,Li J,Jiao J,Xu M,Luo Y,Wang F,Xia Q,Gao Y,Feng Y,Kong X,Sun X

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Wed Jul 21 00:00:00 EDT 2021

NOX4 regulates macrophage apoptosis resistance to induce fibrotic progression.

The Journal of biological chemistry

Larson-Casey JL,Gu L,Kang J,Dhyani A,Carter AB

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

Abraxane-induced bone marrow CD11b⁺ myeloid cell depletion in tumor-bearing mice is visualized by μPET-CT with ⁶⁴Cu-labeled anti-CD11b and prevented by anti-CSF-1.

Theranostics

Cao Q,Huang Q,Wang YA,Li C

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Fri Jul 23 00:00:00 EDT 2021

Inflammatory resolution and vascular barrier restoration after retinal ischemia reperfusion injury.

Journal of neuroinflammation

Abcouwer SF,Shanmugam S,Muthusamy A,Lin CM,Kong D,Hager H,Liu X,Antonetti DA

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

Roles of lung-recruited monocytes and pulmonary Vascular Endothelial Growth Factor (VEGF) in resolving Ventilator-Induced Lung Injury (VILI).

PloS one

Lin CK,Huang TH,Yang CT,Shi CS

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Plaque-associated myeloid cells derive from resident microglia in an Alzheimer's disease model.

The Journal of experimental medicine

Reed-Geaghan EG,Croxford AL,Becher B,Landreth GE

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Mon Apr 06 00:00:00 EDT 2020

Proenkephalin⁺ regulatory T cells expanded by ultraviolet B exposure maintain skin homeostasis with a healing function.

Proceedings of the National Academy of Sciences of the United States of America

Shime H,Odanaka M,Tsuiji M,Matoba T,Imai M,Yasumizu Y,Uraki R,Minohara K,Watanabe M,Bonito AJ,Fukuyama H,Ohkura N,Sakaguchi S,Morita A,Yamazaki S

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Tue Aug 25 00:00:00 EDT 2020

IgE Contributes to Atherosclerosis and Obesity by Affecting Macrophage Polarization, Macrophage Protein Network, and Foam Cell Formation.

Arteriosclerosis, thrombosis, and vascular biology

Zhang X,Li J,Luo S,Wang M,Huang Q,Deng Z,de Febbo C,Daoui A,Liew PX,Sukhova GK,Metso J,Jauhiainen M,Shi GP,Guo J

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Modulation of the functions of myeloid-derived suppressor cells : a new strategy of hydrogen sulfide anti-cancer effects.

British journal of pharmacology

De Cicco P,Ercolano G,Rubino V,Terrazzano G,Ruggiero G,Cirino G,Ianaro A

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Temporospatial distribution and transcriptional profile of retinal microglia in the oxygen-induced retinopathy mouse model.

Glia

Boeck M,Thien A,Wolf J,Hagemeyer N,Laich Y,Yusuf D,Backofen R,Zhang P,Boneva S,Stahl A,Hilgendorf I,Agostini H,Prinz M,Wieghofer P,Schlunck G,Schlecht A,Lange C

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Characterization and regulation of osteoclast precursors following chronic Porphyromonas gingivalis infection.

Journal of leukocyte biology

Zhao Y,Li Z,Su L,Ballesteros-Tato A,Katz J,Michalek SM,Feng X,Zhang P

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Antitumor Effect and Immune Response of Nanosecond Pulsed Electric Fields in Pancreatic Cancer.

Frontiers in oncology

Zhao J,Chen S,Zhu L,Zhang L,Liu J,Xu D,Tian G,Jiang T

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Fri Oct 28 00:00:00 EDT 2022

Effects of Hypertonic Saline and Hydroxyethyl Starch on Myeloid-Derived Suppressor Cells in Hemorrhagic Shock Mice under Secondary Bacterial Attack.

BioMed research international

Jiang JK,Hong LJ,Lu YQ

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Site-1 protease ablation in the osterix-lineage in mice results in bone marrow neutrophilia and hematopoietic stem cell alterations.

Biology open

Patra D,Kim J,Zhang Q,Tycksen E,Sandell LJ

62-5932 was used in Flow cytometry/Cell sorting to show that S1P has a role in the lineage specification of hematopoietic stem cells and/or their progenitors for development of a normal hematopoietic niche.

Tue Jun 23 00:00:00 EDT 2020

Site-1 protease ablation in the osterix-lineage in mice results in bone marrow neutrophilia and hematopoietic stem cell alterations.

Biology open

Patra D,Kim J,Zhang Q,Tycksen E,Sandell LJ

Tue Jun 23 00:00:00 EDT 2020

The impact of Clonorchis sinensis infection on immune response in mice with type II collagen-induced arthritis.

BMC immunology

Li X,Yang Y,Qin S,Kong F,Yan C,Cheng W,Pan W,Yu Q,Hua H,Zheng K,Tang R

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Mon Feb 17 00:00:00 EST 2020

GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype.

Cells

Wang L,Li Y,Wang X,Wang P,Essandoh K,Cui S,Huang W,Mu X,Liu Z,Wang Y,Peng T,Fan GC

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Fri Jan 03 00:00:00 EST 2020

The Transcription Factor Nfix Requires RhoA-ROCK1 Dependent Phagocytosis to Mediate Macrophage Skewing during Skeletal Muscle Regeneration.

Cells

Saclier M,Lapi M,Bonfanti C,Rossi G,Antonini S,Messina G

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Fri Mar 13 00:00:00 EDT 2020

Extracellular miR-146a-5p Induces Cardiac Innate Immune Response and Cardiomyocyte Dysfunction.

ImmunoHorizons

Shimada BK,Yang Y,Zhu J,Wang S,Suen A,Kronstadt SM,Jeyaram A,Jay SM,Zou L,Chao W

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Mon Sep 21 00:00:00 EDT 2020

Resident macrophages acquire innate immune memory in staphylococcal skin infection.

eLife

Feuerstein R,Forde AJ,Lohrmann F,Kolter J,Ramirez NJ,Zimmermann J,Gomez de Agüero M,Henneke P

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Wed Jul 08 00:00:00 EDT 2020

Squalene emulsion-based vaccine adjuvants stimulate CD8 T cell, but not antibody responses, through a RIPK3-dependent pathway.

eLife

Kim EH,Woodruff MC,Grigoryan L,Maier B,Lee SH,Mandal P,Cortese M,Natrajan MS,Ravindran R,Ma H,Merad M,Gitlin AD,Mocarski ES,Jacob J,Pulendran B

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Tue Jun 09 00:00:00 EDT 2020

Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation.

Science advances

Ma C,Yang D,Wang B,Wu C,Wu Y,Li S,Liu X,Lassen K,Dai L,Yang S

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Host Cathelicidin Exacerbates Group B Streptococcus Urinary Tract Infection.

mSphere

Patras KA,Coady A,Babu P,Shing SR,Ha AD,Rooholfada E,Brandt SL,Geriak M,Gallo RL,Nizet V

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Wed Apr 22 00:00:00 EDT 2020

Increased flux through the mevalonate pathway mediates fibrotic repair without injury.

The Journal of clinical investigation

Larson-Casey JL,Vaid M,Gu L,He C,Cai GQ,Ding Q,Davis D,Berryhill TF,Wilson LS,Barnes S,Neighbors JD,Hohl RJ,Zimmerman KA,Yoder BK,Longhini ALF,Hanumanthu VS,Surolia R,Antony VB,Carter AB

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

Differentiated macrophages acquire a pro-inflammatory and cell death-resistant phenotype due to increasing XIAP and p38-mediated inhibition of RipK1.

The Journal of biological chemistry

Rijal D,Ariana A,Wight A,Kim K,Alturki NA,Aamir Z,Ametepe ES,Korneluk RG,Tiedje C,Menon MB,Gaestel M,McComb S,Sad S

Published figure using Ly-6C monoclonal antibody (Product # 62-5932-82) in Flow Cytometry

Fri Jul 27 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Ly-6C1; Ly6c2; lymphocyte antigen 6 complex, locus C; Lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C

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Gene Aliases: AA682074; AA959465; Ly-6C; Ly-6C1; Ly6c; Ly6c1

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UniProt ID: (Mouse) P0CW02

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Entrez Gene ID: (Mouse) 17067

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Performance Guarantee

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Ly-6C Monoclonal Antibody (HK1.4), Super Bright™ 436, eBioscience™

Ly-6C Antibody (62-5932-82) in Flow

Product Details

62-5932-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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