Invitrogen

MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), PerCP-eFluor™ 710, eBioscience™

MHC Class II (I-A/I-E) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 82

MHC Class II (I-A/I-E) Antibody (46-5321-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.03 µg of Rat IgG2b kappa Isotype Control PerCP-eFluor® 710 (Product # 46-4031-82) (left) or 0.03 µg of Anti-Mouse MHC Class II (I-A/I-E) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

46-5321-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.06 µg/test

View 84 publications 84 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M5/114.15.2

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834439

Product Specific Information

Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.

Applications Reported: This M5/114.15.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

IFN-γ signature enables selection of neoadjuvant treatment in patients with stage III melanoma.

The Journal of experimental medicine

Reijers ILM,Rao D,Versluis JM,Menzies AM,Dimitriadis P,Wouters MW,Spillane AJ,Klop WMC,Broeks A,Bosch LJW,Lopez-Yurda M,van Houdt WJ,Rawson RV,Grijpink-Ongering LG,Gonzalez M,Cornelissen S,Bouwman J,Sanders J,Plasmeijer E,Elshot YS,Scolyer RA,van de Wiel BA,Peeper DS,van Akkooi ACJ,Long GV,Blank CU

46-5321-82 was used in Flow cytometry/Cell sorting to investigate whether adding domatinostat to neoadjuvant therapy in stage III melanoma patients, based on their baseline IFN-γ scores, could enhance the IFN-γ response and improve treatment efficacy.

Mon May 01 00:00:00 EDT 2023

Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota.

Cell

Lima-Junior DS,Krishnamurthy SR,Bouladoux N,Collins N,Han SJ,Chen EY,Constantinides MG,Link VM,Lim AI,Enamorado M,Cataisson C,Gil L,Rao I,Farley TK,Koroleva G,Attig J,Yuspa SH,Fischbach MA,Kassiotis G,Belkaid Y

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Jul 08 00:00:00 EDT 2021

CD11b Deficiency Exacerbates Methicillin-Resistant Staphylococcus aureus-Induced Sepsis by Upregulating Inflammatory Responses of Macrophages.

Immune network

Sim H,Jeong D,Kim HI,Pak S,Thapa B,Kwon HJ,Lee K

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Prostaglandin E2 serves a dual role in regulating the migration of dendritic cells.

International journal of molecular medicine

Diao G,Huang J,Zheng X,Sun X,Tian M,Han J,Guo J

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Wip1 regulates the immunomodulatory effects of murine mesenchymal stem cells in type 1 diabetes mellitus via targeting IFN-α/BST2.

Cell death discovery

Zhou N,Liu W,Zhang W,Liu Y,Li X,Wang Y,Zheng R,Zhang Y

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Topical arginase inhibition decreases growth of cutaneous squamous cell carcinoma.

Scientific reports

Mittal A,Wang M,Vidyarthi A,Yanez D,Pizzurro G,Thakral D,Tracy E,Colegio OR

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon May 24 00:00:00 EDT 2021

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Smek1 deficiency exacerbates experimental autoimmune encephalomyelitis by activating proinflammatory microglia and suppressing the IDO1-AhR pathway.

Journal of neuroinflammation

Duan RN,Yang CL,Du T,Liu A,Wang AR,Sun WJ,Li X,Li JX,Yan CZ,Liu QJ

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

Frequent amplification of HDAC genes and efficacy of HDAC inhibitor chidamide and PD-1 blockade combination in soft tissue sarcoma.

Journal for immunotherapy of cancer

Que Y,Zhang XL,Liu ZX,Zhao JJ,Pan QZ,Wen XZ,Xiao W,Xu BS,Hong DC,Guo TH,Shen LJ,Fan WJ,Chen HY,Weng DS,Xu HR,Zhou PH,Zhang YZ,Niu XH,Zhang X

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Early activation of the cardiac CX3CL1/CX3CR1 axis delays β-adrenergic-induced heart failure.

Scientific reports

Flamant M,Mougenot N,Balse E,Le Fèvre L,Atassi F,Gautier EL,Le Goff W,Keck M,Nadaud S,Combadière C,Boissonnas A,Pavoine C

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Sep 09 00:00:00 EDT 2021

PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway.

Frontiers in immunology

Lv N,Jin S,Liang Z,Wu X,Kang Y,Su L,Dong Y,Wang B,Ma T,Shi L

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Feb 28 00:00:00 EST 2022

Epinephrine Production in Th17 Cells and Experimental Autoimmune Encephalitis.

Frontiers in immunology

Yang P,Tian H,Zou YR,Chambon P,Ichinose H,Honig G,Diamond B,Kim SJ

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages.

EMBO molecular medicine

Menga A,Serra M,Todisco S,Riera-Domingo C,Ammarah U,Ehling M,Palmieri EM,Di Noia MA,Gissi R,Favia M,Pierri CL,Porporato PE,Castegna A,Mazzone M

46-5321 was used in Flow cytometry/Cell sorting to evaluate the pharmacological potential of Glutamine synthetase (GS) inhibitors in targeting metastasis.

Wed Oct 07 00:00:00 EDT 2020

Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages.

EMBO molecular medicine

Menga A,Serra M,Todisco S,Riera-Domingo C,Ammarah U,Ehling M,Palmieri EM,Di Noia MA,Gissi R,Favia M,Pierri CL,Porporato PE,Castegna A,Mazzone M

46-5321 was used in Flow cytometry/Cell sorting to evaluate the pharmacological potential of Glutamine synthetase (GS) inhibitors in targeting metastasis.

Wed Oct 07 00:00:00 EDT 2020

Induction of autophagy through CLEC4E in combination with TLR4: an innovative strategy to restrict the survival of Mycobacterium tuberculosis.

Autophagy

Pahari S,Negi S,Aqdas M,Arnett E,Schlesinger LS,Agrewala JN

46-5321-82 was used in Flow Cytometry to invesigate if a unique immunotherapeutic approach involving CLEC4E in conjunction with TLR4 to restrict the survival of mycobacterium tuberculosis through autophagy exists.

Mon Jun 01 00:00:00 EDT 2020

E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions.

International journal of oncology

Huang J,Diao G,Zhang Q,Chen Y,Han J,Guo J

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

IFN-β signalling regulates RAW 264.7 macrophage activation, cytokine production, and killing activity.

Innate immunity

Karimi Y,Giles EC,Vahedi F,Chew MV,Nham T,Loukov D,Lee AJ,Bowdish DM,Ashkar AA

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103⁺CD11b⁺ Dendritic Cells.

Frontiers in immunology

Ko HJ,Hong SW,Verma R,Jung J,Lee M,Kim N,Kim D,Surh CD,Kim KS,Rudra D,Im SH

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Apr 19 00:00:00 EDT 2021

GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype.

Cells

Wang L,Li Y,Wang X,Wang P,Essandoh K,Cui S,Huang W,Mu X,Liu Z,Wang Y,Peng T,Fan GC

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Fri Jan 03 00:00:00 EST 2020

Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells.

Nature communications

Lucas B,White AJ,Cosway EJ,Parnell SM,James KD,Jones ND,Ohigashi I,Takahama Y,Jenkinson WE,Anderson G

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon May 04 00:00:00 EDT 2020

Smooth muscle-derived progenitor cell myofibroblast differentiation through KLF4 downregulation promotes arterial remodeling and fibrosis.

JCI insight

Lu S,Jolly AJ,Strand KA,Dubner AM,Mutryn MF,Moulton KS,Nemenoff RA,Majesky MW,Weiser-Evans MC

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Dec 03 00:00:00 EST 2020

Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection.

Cell reports

Dokoshi T,Zhang LJ,Li F,Nakatsuji T,Butcher A,Yoshida H,Shimoda M,Okada Y,Gallo RL

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Jan 07 00:00:00 EST 2020

Ly6c^Lo non-classical monocytes promote resolution of rhesus rotavirus-mediated perinatal hepatic inflammation.

Scientific reports

Alkhani A,Levy CS,Tsui M,Rosenberg KA,Polovina K,Mattis AN,Mack M,Van Dyken S,Wang BM,Maher JJ,Nijagal A

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

MiR-103 protects from recurrent spontaneous abortion via inhibiting STAT1 mediated M1 macrophage polarization.

International journal of biological sciences

Zhu X,Liu H,Zhang Z,Wei R,Zhou X,Wang Z,Zhao L,Guo Q,Zhang Y,Chu C,Wang L,Li X

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Fri Nov 12 00:00:00 EST 2021

Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.

Journal for immunotherapy of cancer

Neuwelt AJ,Kimball AK,Johnson AM,Arnold BW,Bullock BL,Kaspar RE,Kleczko EK,Kwak JW,Wu MH,Heasley LE,Doebele RC,Li HY,Nemenoff RA,Clambey ET

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Immunomodulatory Effects of Recombinant Mycobacterium smegmatis Expressing Antigen-85B Epitopes in Infected J774A.1 Murine Macrophages.

Pathogens (Basel, Switzerland)

Kadir NA,Acosta A,Sarmiento ME,Norazmi MN

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Sun Nov 29 00:00:00 EST 2020

Artificial Mini Dendritic Cells Boost T Cell-Based Immunotherapy for Ovarian Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Cheng S,Xu C,Jin Y,Li Y,Zhong C,Ma J,Yang J,Zhang N,Li Y,Wang C,Yang Z,Wang Y

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Optimized Protocol for Isolation of Small Extracellular Vesicles from Human and Murine Lymphoid Tissues.

International journal of molecular sciences

Bordas M,Genard G,Ohl S,Nessling M,Richter K,Roider T,Dietrich S,Maaß KK,Seiffert M

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Aug 04 00:00:00 EDT 2020

Skeletal muscle healing by M1-like macrophages produced by transient expression of exogenous GM-CSF.

Stem cell research & therapy

Martins L,Gallo CC,Honda TSB,Alves PT,Stilhano RS,Rosa DS,Koh TJ,Han SW

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Fri Nov 06 00:00:00 EST 2020

Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine.

Nature communications

Campesato LF,Budhu S,Tchaicha J,Weng CH,Gigoux M,Cohen IJ,Redmond D,Mangarin L,Pourpe S,Liu C,Zappasodi R,Zamarin D,Cavanaugh J,Castro AC,Manfredi MG,McGovern K,Merghoub T,Wolchok JD

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Aug 11 00:00:00 EDT 2020

Lactobacillus reuteri 5454 and Bifidobacterium animalis ssp. lactis 5764 improve colitis while differentially impacting dendritic cells maturation and antimicrobial responses.

Scientific reports

Hrdý J,Alard J,Couturier-Maillard A,Boulard O,Boutillier D,Delacre M,Lapadatescu C,Cesaro A,Blanc P,Pot B,Ryffel B,Chamaillard M,Grangette C

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Mar 24 00:00:00 EDT 2020

Gemcitabine Recruits M2-Type Tumor-Associated Macrophages into the Stroma of Pancreatic Cancer.

Translational oncology

Bulle A,Dekervel J,Deschuttere L,Nittner D,Libbrecht L,Janky R,Plaisance S,Topal B,Coosemans A,Lambrechts D,Van Cutsem E,Verslype C,van Pelt J

46-5321-82 was used in Immunohistochemistry (Paraffin) to investigate the interaction between the dense stroma and tumor cells in pancreatic ductal adenocarcinoma (PDAC) using patient-derived tumor xenograft models.

Sun Mar 01 00:00:00 EST 2020

Nuclear imaging-guided PD-L1 blockade therapy increases effectiveness of cancer immunotherapy.

Journal for immunotherapy of cancer

Gao H,Wu Y,Shi J,Zhang X,Liu T,Hu B,Jia B,Wan Y,Liu Z,Wang F

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

A Two-Cell Model for IL-1β Release Mediated by Death-Receptor Signaling.

Cell reports

Donado CA,Cao AB,Simmons DP,Croker BA,Brennan PJ,Brenner MB

46-5321 was used in Flow cytometry/Cell sorting to describe an alternative, two-cell model for IL-1β release where invariant natural killer T (iNKT) cells use the death receptor pathway to instruct antigen-presenting cells to secrete IL-1β.

Tue Apr 07 00:00:00 EDT 2020

CDK4/6 inhibition promotes immune infiltration in ovarian cancer and synergizes with PD-1 blockade in a B cell-dependent manner.

Theranostics

Zhang QF,Li J,Jiang K,Wang R,Ge JL,Yang H,Liu SJ,Jia LT,Wang L,Chen BL

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2020

Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Liu Q,Xie Z,Qiu M,Shim I,Yang Y,Xie S,Yang Q,Wang D,Chen S,Fan T,Ding B,Guo Z,Adah D,Yao X,Zhang Y,Wu H,Wu Z,Wei C,Wang H,Kim HS,Zou Q,Yan Q,Cai Z,Kim JS,Liu LP,Zhang H,Cao Y

46-5321-82 was used in Flow Cytometry to unravel a promising biphotonic strategy for precision treatment of cancer.

Tue Dec 01 00:00:00 EST 2020

Polyanhydride Nanoparticles Induce Low Inflammatory Dendritic Cell Activation Resulting in CD8⁺ T Cell Memory and Delayed Tumor Progression.

International journal of nanomedicine

Darling R,Senapati S,Christiansen J,Liu L,Ramer-Tait AE,Narasimhan B,Wannemuehler M

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

Compartmentalized gut lymph node drainage dictates adaptive immune responses.

Nature

Esterházy D,Canesso MCC,Mesin L,Muller PA,de Castro TBR,Lockhart A,ElJalby M,Faria AMC,Mucida D

46-5321-82 was used in Flow Cytometry to show drainage of gut-draining lymph nodes can resolve conflicting tolerogenic and inflammatory intestinal responses by encouraging antigen targeting to specific gut segments.

Wed May 01 00:00:00 EDT 2019

cGAS/STING axis mediates a topoisomerase II inhibitor-induced tumor immunogenicity.

The Journal of clinical investigation

Wang Z,Chen J,Hu J,Zhang H,Xu F,He W,Wang X,Li M,Lu W,Zeng G,Zhou P,Huang P,Chen S,Li W,Xia LP,Xia X

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Aug 13 00:00:00 EDT 2019

Antiallodynic Effects of Cannabinoid Receptor 2 (CB₂R) Agonists on Retrovirus Infection-Induced Neuropathic Pain.

Pain research & management

Sheng WS,Chauhan P,Hu S,Prasad S,Lokensgard JR

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

MDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment.

Journal for immunotherapy of cancer

Fang DD,Tang Q,Kong Y,Wang Q,Gu J,Fang X,Zou P,Rong T,Wang J,Yang D,Zhai Y

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Nov 28 00:00:00 EST 2019

LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8⁺ T cell tumor-infiltration impairing anti-PD1 therapy.

Nature communications

Pascual-García M,Bonfill-Teixidor E,Planas-Rigol E,Rubio-Perez C,Iurlaro R,Arias A,Cuartas I,Sala-Hojman A,Escudero L,Martínez-Ricarte F,Huber-Ruano I,Nuciforo P,Pedrosa L,Marques C,Braña I,Garralda E,Vieito M,Squatrito M,Pineda E,Graus F,Espejo C,Sahuquillo J,Tabernero J,Seoane J

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Anti-Bacterial Effect of CpG-DNA Involves Enhancement of the Complement Systems.

International journal of molecular sciences

Kim TH,Park J,Kim D,Gautam A,Akauliya M,Kim J,Lee H,Park S,Lee Y,Kwon HJ

46-5321-82 was used in Flow Cytometry to suggest that CpG-DNA enhances the anti-bacterial activity of the immune system by augmenting the levels of complement systems after E. coli K1 infection and triggering other factors as well.

Wed Jul 10 00:00:00 EDT 2019

Macrophage migration inhibitory factor enhances influenza-associated mortality in mice.

JCI insight

Smith CA,Tyrell DJ,Kulkarni UA,Wood S,Leng L,Zemans RL,Bucala R,Goldstein DR

46-5321-82 was used in Flow cytometry/Cell sorting to reveal that migration inhibitory factor (MIF) impairs antiviral host immunity and increases inflammation during influenza infection and suggests that targeting MIF could be therapeutically beneficial during influenza viral infection.

Thu Jul 11 00:00:00 EDT 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5.

The Journal of experimental medicine

Buxadé M,Huerga Encabo H,Riera-Borrull M,Quintana-Gallardo L,López-Cotarelo P,Tellechea M,Martínez-Martínez S,Redondo JM,Martín-Caballero J,Flores JM,Bosch E,Rodríguez-Fernández JL,Aramburu J,López-Rodríguez C

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Nov 05 00:00:00 EST 2018

Effect of immune tolerance induced by immature dendritic cells and CTLA4-Ig on systemic lupus erythematosus: An in vivo study.

Experimental and therapeutic medicine

Huang C,Zhang L,Ling F,Wen S,Luo Y,Liu H,Liu J,Zheng W,Liang M,Sun J,Lin YK

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

FACS isolation of live mouse eosinophils at high purity via a protocol that does not target Siglec F.

Journal of immunological methods

Geslewitz WE,Percopo CM,Rosenberg HF

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Myeloid apolipoprotein E controls dendritic cell antigen presentation and T cell activation.

Nature communications

Bonacina F,Coe D,Wang G,Longhi MP,Baragetti A,Moregola A,Garlaschelli K,Uboldi P,Pellegatta F,Grigore L,Da Dalt L,Annoni A,Gregori S,Xiao Q,Caruso D,Mitro N,Catapano AL,Marelli-Berg FM,Norata GD

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Aug 06 00:00:00 EDT 2018

CD4⁺ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema.

Nature communications

García Nores GD,Ly CL,Cuzzone DA,Kataru RP,Hespe GE,Torrisi JS,Huang JJ,Gardenier JC,Savetsky IL,Nitti MD,Yu JZ,Rehal S,Mehrara BJ

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu May 17 00:00:00 EDT 2018

Lin^-CCR2⁺ hematopoietic stem and progenitor cells overcome resistance to PD-1 blockade.

Nature communications

Flores CT,Wildes TJ,Drake JA,Moore GL,Dean BD,Abraham RS,Mitchell DA

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Wed Oct 17 00:00:00 EDT 2018

A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer.

The Journal of clinical investigation

Berlato C,Khan MN,Schioppa T,Thompson R,Maniati E,Montfort A,Jangani M,Canosa M,Kulbe H,Hagemann UB,Duncan AR,Fletcher L,Wilkinson RW,Powles T,Quezada SA,Balkwill FR

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction.

The Journal of clinical investigation

Nakaya M,Watari K,Tajima M,Nakaya T,Matsuda S,Ohara H,Nishihara H,Yamaguchi H,Hashimoto A,Nishida M,Nagasaka A,Horii Y,Ono H,Iribe G,Inoue R,Tsuda M,Inoue K,Tanaka A,Kuroda M,Nagata S,Kurose H

46-5321 was used in Flow cytometry/Cell sorting to reveal previously unrecognized roles of myofibroblasts in regulating apoptotic engulfment.

Tue Jan 03 00:00:00 EST 2017

Prolonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells.

Frontiers in immunology

Bai WJ,Li CG,Zhang CC,Xu LH,Zeng QZ,Hu B,Hong Z,He XH,Ouyang DY

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice.

Neurobiology of disease

MacPherson KP,Sompol P,Kannarkat GT,Chang J,Sniffen L,Wildner ME,Norris CM,Tansey MG

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Discrimination of the heterogeneity of bone marrow‑derived dendritic cells.

Molecular medicine reports

Wang J,Dai X,Hsu C,Ming C,He Y,Zhang J,Wei L,Zhou P,Wang CY,Yang J,Gong N

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Loss of IRF2BP2 in Microglia Increases Inflammation and Functional Deficits after Focal Ischemic Brain Injury.

Frontiers in cellular neuroscience

Cruz SA,Hari A,Qin Z,Couture P,Huang H,Lagace DC,Stewart AFR,Chen HH

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Mar 17 00:00:00 EDT 2022

Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis.

PloS one

McDonald LT,Johnson SD,Russell DL,Young MRI,LaRue AC

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Sep 25 00:00:00 EDT 2017

Microglial brain region-dependent diversity and selective regional sensitivities to aging.

Nature neuroscience

Grabert K,Michoel T,Karavolos MH,Clohisey S,Baillie JK,Stevens MP,Freeman TC,Summers KM,McColl BW

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Opposite Effects of Coinjection and Distant Injection of Mesenchymal Stem Cells on Breast Tumor Cell Growth.

Stem cells translational medicine

Zheng H,Zou W,Shen J,Xu L,Wang S,Fu YX,Fan W

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Recruited monocytes modulate malaria-induced lung injury through CD36-mediated clearance of sequestered infected erythrocytes.

Journal of leukocyte biology

Lagassé HA,Anidi IU,Craig JM,Limjunyawong N,Poupore AK,Mitzner W,Scott AL

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

Effects of ketanserin on experimental colitis in mice and macrophage function.

International journal of molecular medicine

Xiao J,Shao L,Shen J,Jiang W,Feng Y,Zheng P,Liu F

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Repulsive guidance molecule a blockade exerts the immunoregulatory function in DCs stimulated with ABP and LPS.

Human vaccines & immunotherapeutics

Xu X,Gao Y,Zhai Z,Zhang S,Shan F,Feng J

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Aug 02 00:00:00 EDT 2016

Thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells.

American journal of translational research

Wang H,Hu W,Liang Z,Zeng L,Li J,Yan H,Yang P,Liu Z,Wang L

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Stroke sensitivity in the aged: sex chromosome complement vs. gonadal hormones.

Aging

McCullough LD,Mirza MA,Xu Y,Bentivegna K,Steffens EB,Ritzel R,Liu F

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Epidermal RAF prevents allergic skin disease.

eLife

Raguz J,Jeric I,Niault T,Nowacka JD,Kuzet SE,Rupp C,Fischer I,Biggi S,Borsello T,Baccarini M

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

Internalization and presentation of myelin antigens by the brain endothelium guides antigen-specific T cell migration.

eLife

Lopes Pinheiro MA,Kamermans A,Garcia-Vallejo JJ,van Het Hof B,Wierts L,O'Toole T,Boeve D,Verstege M,van der Pol SM,van Kooyk Y,de Vries HE,Unger WW

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

Adaptive Immune Regulation of Mammary Postnatal Organogenesis.

Developmental cell

Plaks V,Boldajipour B,Linnemann JR,Nguyen NH,Kersten K,Wolf Y,Casbon AJ,Kong N,van den Bijgaart RJ,Sheppard D,Melton AC,Krummel MF,Werb Z

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Sep 14 00:00:00 EDT 2015

The late endosomal adaptor molecule p14 (LAMTOR2) regulates TGFβ1-mediated homeostasis of Langerhans cells.

The Journal of investigative dermatology

Sparber F,Tripp CH,Komenda K,Scheffler JM,Clausen BE,Huber LA,Romani N,Stoitzner P

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Complement C3 mediated targeting of liposomes to granulocytic myeloid derived suppressor cells.

Nanomedicine : nanotechnology, biology, and medicine

Kullberg M,Martinson H,Mann K,Anchordoquy TJ

46-5321 was used in Flow cytometry/Cell sorting to describe the development of a liposomal drug delivery system which targets immune suppressors.

Sat Aug 01 00:00:00 EDT 2015

A genome-wide analysis identifies a notch-RBP-Jκ-IL-7Rα axis that controls IL-17-producing γδ T cell homeostasis in mice.

Journal of immunology (Baltimore, Md. : 1950)

Nakamura M,Shibata K,Hatano S,Sato T,Ohkawa Y,Yamada H,Ikuta K,Yoshikai Y

46-5321 was used in Flow cytometry/Cell sorting to investigate additional roles for the Notch axis in the homeostasis of γδ T cells.

Thu Jan 01 00:00:00 EST 2015

Yersinia pestis targets neutrophils via complement receptor 3.

Cellular microbiology

Merritt PM,Nero T,Bohman L,Felek S,Krukonis ES,Marketon MM

46-5321 was used in Flow cytometry/Cell sorting to identify the factors involved in the targeting of murine splenocytes by Yersinia pestis.

Fri May 01 00:00:00 EDT 2015

Reducing progression of experimental lupus nephritis via inhibition of the B7/CD28 signaling pathway.

Molecular medicine reports

Huang L,Kong Y,Wang J,Sun J,Shi Q,Qiu YH

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Macrophages Contribute to the Spermatogonial Niche in the Adult Testis.

Cell reports

DeFalco T,Potter SJ,Williams AV,Waller B,Kan MJ,Capel B

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Tue Aug 18 00:00:00 EDT 2015

High-fat-diet-mediated dysbiosis promotes intestinal carcinogenesis independently of obesity.

Nature

Schulz MD,Atay C,Heringer J,Romrig FK,Schwitalla S,Aydin B,Ziegler PK,Varga J,Reindl W,Pommerenke C,Salinas-Riester G,Böck A,Alpert C,Blaut M,Polson SC,Brandl L,Kirchner T,Greten FR,Polson SW,Arkan MC

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Oct 23 00:00:00 EDT 2014

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy.

PloS one

Xiao GY,Liu IH,Cheng CC,Chang CC,Lee YH,Cheng WT,Wu SC

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu May 28 00:00:00 EDT 2015

Assessing the role of STAT3 in DC differentiation and autologous DC immunotherapy in mouse models of GBM.

PloS one

Assi H,Espinosa J,Suprise S,Sofroniew M,Doherty R,Zamler D,Lowenstein PR,Castro MG

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Fri Jan 23 00:00:00 EST 2015

CD30 is required for activation of a unique subset of interleukin-17A-producing γδ T cells in innate immunity against Mycobacterium bovis Bacillus Calmette-Guerin infection.

Infection and immunity

Guo Y,Sun X,Shibata K,Yamada H,Muta H,Podack ER,Yoshikai Y

46-5321 was used in Flow cytometry/Cell sorting to demonstrate the involvement of CD30 signalling in the production of IL-17A by γδ T cells following BCG infection.

Tue Oct 01 00:00:00 EDT 2013

Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages.

Blood

He H,Xu J,Warren CM,Duan D,Li X,Wu L,Iruela-Arispe ML

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Thu Oct 11 00:00:00 EDT 2012

Isolation and characterization of novel murine epiphysis derived mesenchymal stem cells.

PloS one

Cheng CC,Lian WS,Hsiao FS,Liu IH,Lin SP,Lee YH,Chang CC,Xiao GY,Huang HY,Cheng CF,Cheng WT,Wu SC

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2012

Mycobacterium bovis BCG decreases MHC-II expression in vivo on murine lung macrophages and dendritic cells during aerosol infection.

Cellular immunology

Pecora ND,Fulton SA,Reba SM,Drage MG,Simmons DP,Urankar-Nagy NJ,Boom WH,Harding CV

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 46-5321-82) in Flow Cytometry

Fri Jan 02 00:00:00 EST 2009

Bioinformatics

Protein Aliases: A alpha; cell surface glycoprotein; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; histocompatibility 2, class II antigen A, alpha; histocompatibility 2, class II antigen A, beta 1; I-Ab; I-Ad; I-Aq; I-E beta MHC class II; I-Ed; I-Ek; IAalpha; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; major histocompatibility protein class II alpha chain; MHC class 2; MHC class II antigen A alpha isoform-004; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC H2-IE-beta cell surface glycoprotein; MHC-II I-A(d) alpha; response to metastatic cancers 1

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Gene Aliases: Aalpha; Abeta; AI845868; H-2Aa; H-2Ab; H-2Eb; H2-Aa; H2-Ab; H2-Ab1; H2-iabeta; H2Aa; H2Eb; I-Aalpha; I-Abeta; Ia-1; Ia-2; Ia-4; Ia1; Ia2; Ia4; IAalpha; IAb; Rmcs1

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UniProt ID: (Mouse) P14434, (Mouse) P14483

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Entrez Gene ID: (Mouse) 14960, (Mouse) 14961, (Mouse) 14969

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Function(s)

protein binding peptide antigen binding protein heterodimerization activity transcription factor binding toxic substance binding ubiquitin protein ligase binding repressing transcription factor binding major histocompatibility complex protein

Process(es)

immune system process antigen processing and presentation of peptide or polysaccharide antigen via MHC class II immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II response to interferon-gamma negative regulation of T cell proliferation positive regulation of T cell differentiation antigen processing and presentation of peptide antigen B cell affinity maturation immunoglobulin production involved in immunoglobulin mediated immune response humoral immune response mediated by circulating immunoglobulin positive regulation of antigen processing and presentation positive regulation of T-helper 1 type immune response positive regulation of alpha-beta T cell activation cellular response to interferon-gamma

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MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), PerCP-eFluor™ 710, eBioscience™

MHC Class II (I-A/I-E) Antibody (46-5321-82) in Flow

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46-5321-82

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