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Invitrogen
MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Super Bright™ 645, eBioscience™
FIGURE: 1 / 32
MHC Class II (I-A/I-E) Antibody (64-5321-82) in Flow
Staining of C57BL/6 splenocytes with Anti-Mouse CD3 FITC (Product # 11-0031-82) and Rat IgG2b K Isotype Control (Product # 64-4031-82) (left) or 0.06 µg of Anti-Mouse MHC Class II (I-A/I-E) Super Bright 645 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
64-5321-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
M5/114.15.2
Conjugate
Excitation/Emission Max
414/645 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2662402
Product Specific Information
Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Applications Reported: This M5/114.15.2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: A alpha; cell surface glycoprotein; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; histocompatibility 2, class II antigen A, alpha; histocompatibility 2, class II antigen A, beta 1; I-Ab; I-Ad; I-Aq; I-E beta MHC class II; I-Ed; I-Ek; IAalpha; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; major histocompatibility protein class II alpha chain; MHC class 2; MHC class II antigen A alpha isoform-004; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC H2-IE-beta cell surface glycoprotein; MHC-II I-A(d) alpha; response to metastatic cancers 1
Gene Aliases: Aalpha; Abeta; AI845868; H-2Aa; H-2Ab; H-2Eb; H2-Aa; H2-Ab; H2-Ab1; H2-iabeta; H2Aa; H2Eb; I-Aalpha; I-Abeta; Ia-1; Ia-2; Ia-4; Ia1; Ia2; Ia4; IAalpha; IAb; Rmcs1
UniProt ID: (Mouse) P14434, (Mouse) P14483
Entrez Gene ID: (Mouse) 14960, (Mouse) 14961, (Mouse) 14969
immune system process
antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
immune response
antigen processing and presentation
antigen processing and presentation of exogenous peptide antigen via MHC class II
response to interferon-gamma
negative regulation of T cell proliferation
positive regulation of T cell differentiation
antigen processing and presentation of peptide antigen
B cell affinity maturation
immunoglobulin production involved in immunoglobulin mediated immune response
humoral immune response mediated by circulating immunoglobulin
positive regulation of antigen processing and presentation
positive regulation of T-helper 1 type immune response
positive regulation of alpha-beta T cell activation
cellular response to interferon-gamma
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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