Invitrogen

MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Alexa Fluor™ 532, eBioscience™

MHC Class II (I-A/I-E) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 32

MHC Class II (I-A/I-E) Antibody (58-5321-82) in Flow

BALB/c mouse splenocytes were stained with CD3e Monoclonal Antibody, APC (Product # 17-0031-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Alexa Fluor 532 (Product # 58-4031-80) (left) or 0.25 µg of MHC Class II Monoclonal Antibody, Alexa Fluor 532 (right). Cells in the lymphocyte gate were used for analysis.

MHC Class II (I-A/I-E) Antibody in Immunohistochemistry (IHC)

MHC Class II (I-A/I-E) Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

58-5321-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.5 µg/test

View 35 publications 35 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 532, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M5/114.15.2

Conjugate

Alexa Fluor™ 532 Alexa Fluor™ 532 Alexa Fluor™ 532

Excitation/Emission Max

534/553 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2811913

Product Specific Information

Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.

Applications Reported: This m5/114.15.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This m5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser

Target Information

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A alpha; cell surface glycoprotein; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; histocompatibility 2, class II antigen A, alpha; histocompatibility 2, class II antigen A, beta 1; I-Ab; I-Ad; I-Aq; I-E beta MHC class II; I-Ed; I-Ek; IAalpha; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; major histocompatibility protein class II alpha chain; MHC class 2; MHC class II antigen A alpha isoform-004; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC H2-IE-beta cell surface glycoprotein; MHC-II I-A(d) alpha; response to metastatic cancers 1

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Gene Aliases: Aalpha; Abeta; AI845868; H-2Aa; H-2Ab; H-2Eb; H2-Aa; H2-Ab; H2-Ab1; H2-iabeta; H2Aa; H2Eb; I-Aalpha; I-Abeta; Ia-1; Ia-2; Ia-4; Ia1; Ia2; Ia4; IAalpha; IAb; Rmcs1

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UniProt ID: (Mouse) P14434, (Mouse) P14483

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Entrez Gene ID: (Mouse) 14960, (Mouse) 14961, (Mouse) 14969

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Function(s)

protein binding peptide antigen binding protein heterodimerization activity transcription factor binding toxic substance binding ubiquitin protein ligase binding repressing transcription factor binding major histocompatibility complex protein

Process(es)

immune system process antigen processing and presentation of peptide or polysaccharide antigen via MHC class II immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II response to interferon-gamma negative regulation of T cell proliferation positive regulation of T cell differentiation antigen processing and presentation of peptide antigen B cell affinity maturation immunoglobulin production involved in immunoglobulin mediated immune response humoral immune response mediated by circulating immunoglobulin positive regulation of antigen processing and presentation positive regulation of T-helper 1 type immune response positive regulation of alpha-beta T cell activation cellular response to interferon-gamma

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Alexa Fluor™ 532, eBioscience™

MHC Class II (I-A/I-E) Antibody (58-5321-82) in Flow

Product Details

58-5321-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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