Invitrogen

CD140b (PDGFRB) Monoclonal Antibody (APB5), PE, eBioscience™

21 Published Figures

38 References

View all (90) PDGFRB (CD140b) antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (21)

CD140b (PDGFRB) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 22

CD140b (PDGFRB) Antibody (12-1402-81) in Flow

Staining of NIH/3T3 cell line with 0.5 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.5 µg of Anti-Mouse CD140b (PDGF Receptor b) PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-1402-81

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 36 publications 36 publications

In vitro Assay (IV)

- -

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

APB5

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_529484

Product Specific Information

Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.

Applications Reported: This APB5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

PDGFRB is a receptor tyrosine kinase and has been implicated in atherogenesis and in cell transformation by the SIS oncogene. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses PDGFRB (CD140b) to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Type H vessel/platelet-derived growth factor receptor β⁺ perivascular cell disintegration is involved in vascular injury and bone loss in radiation-induced bone damage.

Cell proliferation

Li J,Chen X,Ren L,Chen X,Wu T,Wang Y,Ren X,Cheng B,Xia J

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Establishment of a pure culture of immature Sertoli cells by PDGFRA staining and cell sorting.

Molecular reproduction and development

Malolina EA,Galiakberova AA,Dashinimaev EB,Kulibin AY

12-1402-81 was used in Flow cytometry/Cell sorting to demonstrate a novel approach to establish a pure Sertoli cell culture from immature mouse testes.

Sun May 01 00:00:00 EDT 2022

Cellular dormancy in minimal residual disease following targeted therapy.

Breast cancer research : BCR

Ruth JR,Pant DK,Pan TC,Seidel HE,Baksh SC,Keister BA,Singh R,Sterner CJ,Bakewell SJ,Moody SE,Belka GK,Chodosh LA

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Distinct Fibroblast Lineages Give Rise to NG2+ Pericyte Populations in Mouse Skin Development and Repair.

Frontiers in cell and developmental biology

Goss G,Rognoni E,Salameti V,Watt FM

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Wed Mar 02 00:00:00 EST 2022

Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease.

FEBS open bio

Lee LL,Khakoo AY,Chintalgattu V

12-1402 was used in Flow cytometry/Cell sorting to characterize cardiac PCs under in vitro disease conditions and supports the hypothesis that cardiac PCs are key vasoactive cells that can regulate blood flow in the heart.

Fri Jan 01 00:00:00 EST 2021

Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease.

FEBS open bio

Lee LL,Khakoo AY,Chintalgattu V

Fri Jan 01 00:00:00 EST 2021

Tissue-Resident PDGFRα⁺ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner.

Cell reports

Santini MP,Malide D,Hoffman G,Pandey G,D'Escamard V,Nomura-Kitabayashi A,Rovira I,Kataoka H,Ochando J,Harvey RP,Finkel T,Kovacic JC

12-1402 was used in Flow cytometry/Cell sorting to support a context-dependent "yin-yang" biology of tissue-resident mesenchymal progenitor cells, which possess an innate ability to limit injury expansion while also promoting fibrosis in an unfavorable environment.

Tue Jan 14 00:00:00 EST 2020

Dual lineage tracing identifies intermediate mesenchymal stage for endocardial contribution to fibroblasts, coronary mural cells, and adipocytes.

The Journal of biological chemistry

Huang X,Feng T,Jiang Z,Meng J,Kou S,Lu Z,Chen W,Lin CP,Zhou B,Zhang H

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Fri May 31 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

Mural cell-derived laminin-α5 plays a detrimental role in ischemic stroke.

Acta neuropathologica communications

Nirwane A,Johnson J,Nguyen B,Miner JH,Yao Y

12-1402 was used in Flow cytometry/Cell sorting to investigate whether mural cell-derived laminin-α5 is involved in vascular integrity regulation.

Mon Feb 18 00:00:00 EST 2019

Adventitial Sca1+ Cells Transduced With ETV2 Are Committed to the Endothelial Fate and Improve Vascular Remodeling After Injury.

Arteriosclerosis, thrombosis, and vascular biology

Le Bras A,Yu B,Issa Bhaloo S,Hong X,Zhang Z,Hu Y,Xu Q

12-1402 was used in Flow cytometry/Cell sorting to provide evidence that the genetic manipulation of vascular progenitors is a promising approach to improve vascular function after endothelial injury.

Mon Jan 01 00:00:00 EST 2018

Pulmonary pericytes regulate lung morphogenesis.

Nature communications

Kato K,Diéguez-Hurtado R,Park DY,Hong SP,Kato-Azuma S,Adams S,Stehling M,Trappmann B,Wrana JL,Koh GY,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Nature communications

Corallino S,Malinverno C,Neumann B,Tischer C,Palamidessi A,Frittoli E,Panagiotakopoulou M,Disanza A,Malet-Engra G,Nastaly P,Galli C,Luise C,Bertalot G,Pece S,Di Fiore PP,Gauthier N,Ferrari A,Maiuri P,Scita G

12-1402 was used in Flow cytometry/Cell sorting to identify RAB35 as necessary for the formation of growth factor-induced waves of circular dorsal ruffles, apically restricted actin-rich migratory protrusions.

Mon Apr 16 00:00:00 EDT 2018

Imatinib Inhibits the Renewal and Tumorigenicity of CT-26 Colon Cancer Cells after Cytoreductive Treatment with Doxorubicin.

Archivum immunologiae et therapiae experimentalis

Przybyszewska M,Miłoszewska J,Kotlarz A,Swoboda P,Pyśniak K,Szczepek W,Kaczmarek Ł,Markowicz S

12140281 was used in flow cytometry to assess if drugs that target receptor tyrosine kinases silence or eliminate colorectal cancer cells that are refractory to conventional treatment

Wed Feb 01 00:00:00 EST 2017

Laminin differentially regulates the stemness of type I and type II pericytes.

Stem cell research & therapy

Gautam J,Nirwane A,Yao Y

Tue Feb 07 00:00:00 EST 2017

Neonatal pancreatic pericytes support β-cell proliferation.

Molecular metabolism

Epshtein A,Rachi E,Sakhneny L,Mizrachi S,Baer D,Landsman L

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Mammary tumor-derived CCL2 enhances pro-metastatic systemic inflammation through upregulation of IL1β in tumor-associated macrophages.

Oncoimmunology

Kersten K,Coffelt SB,Hoogstraat M,Verstegen NJM,Vrijland K,Ciampricotti M,Doornebal CW,Hau CS,Wellenstein MD,Salvagno C,Doshi P,Lips EH,Wessels LFA,de Visser KE

12-1402 was used in Flow cytometry/Cell sorting to demonstrate mammary tumor-derived CCL2 can facilitate the breast cancer-induced pro-metastatic systemic inflammatory gamma delta T cell-IL17–neutrophil axis.

Sat Jan 29 00:00:00 EST 2022

Age-dependent modulation of vascular niches for haematopoietic stem cells.

Nature

Kusumbe AP,Ramasamy SK,Itkin T,Mäe MA,Langen UH,Betsholtz C,Lapidot T,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2016

Cardiac Fibro-Adipocyte Progenitors Express Desmosome Proteins and Preferentially Differentiate to Adipocytes Upon Deletion of the Desmoplakin Gene.

Circulation research

Lombardi R,Chen SN,Ruggiero A,Gurha P,Czernuszewicz GZ,Willerson JT,Marian AJ

12140281 was used in flow cytometry and immunocytochemistry to discuss the cellular origins of fibroadipocytes in arrhythmogenic cardiomyopathy

Fri Jun 24 00:00:00 EDT 2016

Cardiac Fibro-Adipocyte Progenitors Express Desmosome Proteins and Preferentially Differentiate to Adipocytes Upon Deletion of the Desmoplakin Gene.

Circulation research

Lombardi R,Chen SN,Ruggiero A,Gurha P,Czernuszewicz GZ,Willerson JT,Marian AJ

12140281 was used in flow cytometry and immunocytochemistry to discuss the cellular origins of fibroadipocytes in arrhythmogenic cardiomyopathy

Fri Jun 24 00:00:00 EDT 2016

Paracrine Wnt1 Drives Interstitial Fibrosis without Inflammation by Tubulointerstitial Cross-Talk.

Journal of the American Society of Nephrology : JASN

Maarouf OH,Aravamudhan A,Rangarajan D,Kusaba T,Zhang V,Welborn J,Gauvin D,Hou X,Kramann R,Humphreys BD

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Analysis of the brain mural cell transcriptome.

Scientific reports

He L,Vanlandewijck M,Raschperger E,Andaloussi Mäe M,Jung B,Lebouvier T,Ando K,Hofmann J,Keller A,Betsholtz C

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.

Nature communications

Chen Q,Zhang H,Liu Y,Adams S,Eilken H,Stehling M,Corada M,Dejana E,Zhou B,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Fri Aug 12 00:00:00 EDT 2016

The role of pericytic laminin in blood brain barrier integrity maintenance.

Scientific reports

Gautam J,Zhang X,Yao Y

12140281 was used in flow cytometry to examine the function of pericyte-derived laminin in vascular integrity

Thu Nov 03 00:00:00 EDT 2016

Laminin regulates PDGFRβ(+) cell stemness and muscle development.

Nature communications

Yao Y,Norris EH,Mason CE,Strickland S

12-1402 was used in Flow cytometry/Cell sorting to show that laminin has a critical role in the control of PDGFRBeta(+) cell stemness and that PDGFRBeta(+) may be a drug target for treatment of muscular dystrophy.

Tue May 03 00:00:00 EDT 2016

Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney.

JCI insight

Sato Y,Mii A,Hamazaki Y,Fujita H,Nakata H,Masuda K,Nishiyama S,Shibuya S,Haga H,Ogawa O,Shimizu A,Narumiya S,Kaisho T,Arita M,Yanagisawa M,Miyasaka M,Sharma K,Minato N,Kawamoto H,Yanagita M

12-1402 was used in Flow cytometry/Cell sorting to show how the inhibition of tertiary lymphoid tissue may constitute a therapeutic approach for treating acute kidney injury in the elderly.

Thu Jul 21 00:00:00 EDT 2016

Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle.

The Journal of clinical investigation

Quattrocelli M,Swinnen M,Giacomazzi G,Camps J,Barthélemy I,Ceccarelli G,Caluwé E,Grosemans H,Thorrez L,Pelizzo G,Muijtjens M,Verfaillie CM,Blot S,Janssens S,Sampaolesi M

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Crouch EE,Liu C,Silva-Vargas V,Doetsch F

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Wed Mar 18 00:00:00 EDT 2015

Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer.

Cell cycle (Georgetown, Tex.)

Qin Y,Qin J,Zhou C,Li J,Gao WQ

12-1402 was used in Flow cytometry/Cell sorting to demonstrates that ESCs can be generated from the adipose tissue by somatic cell nuclear transfer (SCNT) and suggests that ADCs can be a new donor-cell type for potential therapeutic cloning.

Tue Jan 26 00:00:00 EST 2016

Mouse dental pulp stem cells support human umbilical cord blood-derived hematopoietic stem/progenitor cells in vitro.

Cell transplantation

Nakatsuka R,Matsuoka Y,Uemura Y,Sumide K,Iwaki R,Takahashi M,Fujioka T,Sasaki Y,Sonoda Y

12-1402 was used in Flow cytometry/Cell sorting to demonstrate that prospectively isolated murine PDGFRα and Sca-1 double-positive dental pulp stem cells could support primitive human CD34+ and CD34- hematopoietic stem cells in vitro.

Wed Sep 16 00:00:00 EDT 2015

Quantitative assessment of angiogenesis, perfused blood vessels and endothelial tip cells in the postnatal mouse brain.

Nature protocols

Wälchli T,Mateos JM,Weinman O,Babic D,Regli L,Hoerstrup SP,Gerhardt H,Schwab ME,Vogel J

12-1402-81 was used in flow cytometry to describe a method that allows the assessment of these distinct angiogenic steps together with antibody-based protein detection in the postnatal mouse brain.

Thu Jan 01 00:00:00 EST 2015

Pericytes derived from adipose-derived stem cells protect against retinal vasculopathy.

PloS one

Mendel TA,Clabough EB,Kao DS,Demidova-Rice TN,Durham JT,Zotter BC,Seaman SA,Cronk SM,Rakoczy EP,Katz AJ,Herman IM,Peirce SM,Yates PA

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2014

Label retention identifies a multipotent mesenchymal stem cell-like population in the postnatal thymus.

PloS one

Osada M,Singh VJ,Wu K,Sant'Angelo DB,Pezzano M

12-1402 was used in Flow cytometry/Cell sorting to investigate whether adult stem cells contribute to the maintenance of thymic epithelial microenvironments, revealing a multipotent mesenchymal stem cell-like population in the postnatal thymus.

Tue Sep 30 00:00:00 EDT 2014

Intratumoral IL-12 gene therapy results in the crosspriming of Tc1 cells reactive against tumor-associated stromal antigens.

Molecular therapy : the journal of the American Society of Gene Therapy

Zhao X,Bose A,Komita H,Taylor JL,Kawabe M,Chi N,Spokas L,Lowe DB,Goldbach C,Alber S,Watkins SC,Butterfield LH,Kalinski P,Kirkwood JM,Storkus WJ

12-1402 was used in Flow cytometry/Cell sorting to study how TASA and their derivative peptides may prove useful in vaccine formulations against solid cancers.

Fri Apr 01 00:00:00 EDT 2011

Assessment of the myogenic stem cell compartment following transplantation of Pax3/Pax7-induced embryonic stem cell-derived progenitors.

Stem cells (Dayton, Ohio)

Darabi R,Santos FN,Filareto A,Pan W,Koene R,Rudnicki MA,Kyba M,Perlingeiro RC

12-1402 was used in Flow cytometry/Cell sorting to investigate Pax3/Pax7-induced embryonic stem cell-derived progenitors as a therapeutic application of ES-derived myogenic progenitors in muscle disorders.

Sun May 01 00:00:00 EDT 2011

Imatinib has deleterious effects on differentiating spermatogonia while sparing spermatogonial stem cell self renewal.

Reproductive toxicology (Elmsford, N.Y.)

Heim C,Minniear K,Dann CT

12-1402 was used in Immunohistochemistry to support the possibility of utilizing germline stem cell cultures for preclinical drug tests.

Sun May 01 00:00:00 EDT 2011

Differential contribution to neuroendocrine tumorigenesis of parallel egfr signaling in cancer cells and pericytes.

Genes & cancer

Nolan-Stevaux O,Truitt MC,Pahler JC,Olson P,Guinto C,Lee DC,Hanahan D

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 12-1402-81) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2010

A protocol for phenotypic detection and characterization of vascular cells of different origins in a lung neovascularization model in rodents.

Nature protocols

Jones RC,Capen DE,Cohen KS,Munn LL,Jain RK,Duda DG

12-1402 was used in Flow cytometry/Cell sorting to describe a protocol to immunophenotype vascular cells by high-resolution imaging and by fluorescence-activated flow cytometry in a lung neovascularization model.

Tue Nov 18 00:00:00 EST 2008

Characterization of fibroblasts responsible for cartilage destruction in arthritis.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Treese C,Mittag A,Lange F,Tarnok A,Loesche A,Emmrich F,Lehmann J,Sack U

12-1402 was used in Flow cytometry/Cell sorting to show that LS48 is an excellent tool to gain a deeper understanding of synovial fibroblasts in rheumatoid arthritis.

Tue Apr 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b; CD140b antigen; PDGF beta chain; PDGF Receptor beta; PDGF-R-beta; PDGFR-1; PDGFR-beta; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1

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Gene Aliases: AI528809; CD140b; Pdgfr; PDGFR-1; Pdgfr1; Pdgfrb

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UniProt ID: (Mouse) P05622

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Entrez Gene ID: (Mouse) 18596

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity signal transducer activity platelet-derived growth factor-activated receptor activity platelet-derived growth factor beta-receptor activity receptor binding platelet-derived growth factor receptor binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding vascular endothelial growth factor binding phosphatidylinositol 3-kinase binding platelet-derived growth factor binding transmembrane signal receptor

Process(es)

Performance Guarantee

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CD140b (PDGFRB) Monoclonal Antibody (APB5), PE, eBioscience™

CD140b (PDGFRB) Antibody (12-1402-81) in Flow

Product Details

12-1402-81

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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