Invitrogen

CD140b (PDGFRB) Monoclonal Antibody (APB5), Functional Grade, eBioscience™

CD140b (PDGFRB) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 88

CD140b (PDGFRB) Antibody (16-1402-82) in Flow

Staining of NIH-3T3 cell line with 0.5 µg of Rat IgG2a kappa Isotype Control Functional Grade Purified (Product # 16-4321-82) (open histogram) or 0.5 µg of Anti-Mouse CD140b (PDGF Receptor b) Functional Grade Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis.

CD140b (PDGFRB) Antibody in Immunohistochemistry (IHC)

CD140b (PDGFRB) Antibody in Immunocytochemistry (ICC/IF)

CD140b (PDGFRB) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD140b (PDGFRB) Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))

CD140b (PDGFRB) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD140b (PDGFRB) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

CD140b (PDGFRB) Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)

CD140b (PDGFRB) Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD140b (PDGFRB) Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD140b (PDGFRB) Antibody in Western Blot, Immunohistochemistry (WB, IHC)

CD140b (PDGFRB) Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))

Product Image Gallery

Product Details

16-1402-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 34 publications 34 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 12 publications 12 publications

Flow Cytometry (Flow)

1 µg/test

View 19 publications 19 publications

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

APB5

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469070

Product Specific Information

Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.

Applications Reported: The APB5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of ligand binding.

Applications Tested: The APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

PDGFRB is a receptor tyrosine kinase and has been implicated in atherogenesis and in cell transformation by the SIS oncogene. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses PDGFRB (CD140b) to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Type H vessel/platelet-derived growth factor receptor β⁺ perivascular cell disintegration is involved in vascular injury and bone loss in radiation-induced bone damage.

Cell proliferation

Li J,Chen X,Ren L,Chen X,Wu T,Wang Y,Ren X,Cheng B,Xia J

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Embryonic Pericytes Promote Microglial Homeostasis and Their Effects on Neural Progenitors in the Developing Cerebral Cortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Hattori Y,Itoh H,Tsugawa Y,Nishida Y,Kurata K,Uemura A,Miyata T

16-1402-82 was used in Flow cytometry/Cell sorting to provide evidence that pericytes function not only in the maintenance of cerebral microcirculation and blood brain barrier (BBB) integrity but also in microglial homeostasis in the developing cerebral walls.

Wed Jan 19 00:00:00 EST 2022

Release of stem cells from quiescence reveals gliogenic domains in the adult mouse brain.

Science (New York, N.Y.)

Delgado AC,Maldonado-Soto AR,Silva-Vargas V,Mizrak D,von Känel T,Tan KR,Paul A,Madar A,Cuervo H,Kitajewski J,Lin CS,Doetsch F

16-1402-82 was used in Flow Cytometry to show that platelet-derived growth factor receptor beta (PDGFRβ) is expressed by adult ventricular-subventricular zone neural stem cells that generate olfactory bulb interneurons and glia.

Fri Jun 11 00:00:00 EDT 2021

Cellular dormancy in minimal residual disease following targeted therapy.

Breast cancer research : BCR

Ruth JR,Pant DK,Pan TC,Seidel HE,Baksh SC,Keister BA,Singh R,Sterner CJ,Bakewell SJ,Moody SE,Belka GK,Chodosh LA

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Distinct Fibroblast Lineages Give Rise to NG2+ Pericyte Populations in Mouse Skin Development and Repair.

Frontiers in cell and developmental biology

Goss G,Rognoni E,Salameti V,Watt FM

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Wed Mar 02 00:00:00 EST 2022

Generation and characterization of a Myh6-driven Cre knockin mouse line.

Transgenic research

Huang X,Yan L,Kou S,Meng J,Lu Z,Lin CP,Liu C,Zhang H

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunocytochemistry

Wed Dec 01 00:00:00 EST 2021

Direct contribution of skeletal muscle mesenchymal progenitors to bone repair.

Nature communications

Julien A,Kanagalingam A,Martínez-Sarrà E,Megret J,Luka M,Ménager M,Relaix F,Colnot C

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunohistochemistry

Mon May 17 00:00:00 EDT 2021

Perivascular cell-derived extracellular vesicles stimulate colorectal cancer revascularization after withdrawal of antiangiogenic drugs.

Journal of extracellular vesicles

Huang M,Chen M,Qi M,Ye G,Pan J,Shi C,Yang Y,Zhao L,Mo X,Zhang Y,Li Y,Zhong J,Lu W,Li X,Zhang J,Lin J,Luo L,Liu T,Tang PM,Hong A,Cao Y,Ye W,Zhang D

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunocytochemistry

Sat May 01 00:00:00 EDT 2021

Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease.

FEBS open bio

Lee LL,Khakoo AY,Chintalgattu V

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

PDGF Receptor Alpha Signaling Is Key for Müller Cell Homeostasis Functions.

International journal of molecular sciences

Díaz-Lezama N,Wolf A,Koch S,Pfaller AM,Biber J,Guillonneau X,Langmann T,Grosche A

16-1402-82 was used in Functional assay to imply that targeting PDGF to treat retinal neovascular diseases may have short-term beneficial effects, but may elicit unwarranted side effects given the putative negative effects on Müller cell homeostatic functions potentially interfering with a long-term positive outcome.

Mon Jan 25 00:00:00 EST 2021

Single-cell profiling reveals an endothelium-mediated immunomodulatory pathway in the eye choroid.

The Journal of experimental medicine

Lehmann GL,Hanke-Gogokhia C,Hu Y,Bareja R,Salfati Z,Ginsberg M,Nolan DJ,Mendez-Huergo SP,Dalotto-Moreno T,Wojcinski A,Ochoa F,Zeng S,Cerliani JP,Panagis L,Zager PJ,Mullins RF,Ogura S,Lutty GA,Bang J,Zippin JH,Romano C,Rabinovich GA,Elemento O,Joyner AL,Rafii S,Rodriguez-Boulan E,Benedicto I

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunocytochemistry

Mon Jun 01 00:00:00 EDT 2020

Cell profiling of mouse acute kidney injury reveals conserved cellular responses to injury.

Proceedings of the National Academy of Sciences of the United States of America

Kirita Y,Wu H,Uchimura K,Wilson PC,Humphreys BD

16-1402-82 was used in Immunocytochemistry to provide a detailed description of cellular responses after injury and suggests that the FR-PTC state may represent a therapeutic target to improve repair.

Tue Jul 07 00:00:00 EDT 2020

TGFβ1 orchestrates renal fibrosis following Escherichia coli pyelonephritis.

Physiological reports

Hreha TN,Collins CA,Daugherty AL,Twentyman J,Paluri N,Hunstad DA

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunohistochemistry

Sun Mar 01 00:00:00 EST 2020

Angiomodulin (IGFBP7) is a cerebral specific angiocrine factor, but is probably not a blood-brain barrier inducer.

Fluids and barriers of the CNS

Bar O,Gelb S,Atamny K,Anzi S,Ben-Zvi A

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunocytochemistry

Wed Apr 01 00:00:00 EDT 2020

Angiomodulin (IGFBP7) is a cerebral specific angiocrine factor, but is probably not a blood-brain barrier inducer.

Fluids and barriers of the CNS

Bar O,Gelb S,Atamny K,Anzi S,Ben-Zvi A

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunocytochemistry

Wed Apr 01 00:00:00 EDT 2020

A conditionally immortalized Gli1-positive kidney mesenchymal cell line models myofibroblast transition.

American journal of physiology. Renal physiology

Ó hAinmhire E,Wu H,Muto Y,Donnelly EL,Machado FG,Fan LX,Chang-Panesso M,Humphreys BD

16-1402-82 was used in Immunohistochemistry to isolate kidney-Gli1 cells and observe their differentiation to myofibroblasts.

Tue Jan 01 00:00:00 EST 2019

Claudin-12 is not required for blood-brain barrier tight junction function.

Fluids and barriers of the CNS

Castro Dias M,Coisne C,Baden P,Enzmann G,Garrett L,Becker L,Hölter SM,Hrabě de Angelis M,Deutsch U,Engelhardt B

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Thu Sep 12 00:00:00 EDT 2019

Claudin-12 is not required for blood-brain barrier tight junction function.

Fluids and barriers of the CNS

Castro Dias M,Coisne C,Baden P,Enzmann G,Garrett L,Becker L,Hölter SM,Hrabě de Angelis M,Deutsch U,Engelhardt B

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Thu Sep 12 00:00:00 EDT 2019

Dual lineage tracing identifies intermediate mesenchymal stage for endocardial contribution to fibroblasts, coronary mural cells, and adipocytes.

The Journal of biological chemistry

Huang X,Feng T,Jiang Z,Meng J,Kou S,Lu Z,Chen W,Lin CP,Zhou B,Zhang H

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Fri May 31 00:00:00 EDT 2019

Dual lineage tracing identifies intermediate mesenchymal stage for endocardial contribution to fibroblasts, coronary mural cells, and adipocytes.

The Journal of biological chemistry

Huang X,Feng T,Jiang Z,Meng J,Kou S,Lu Z,Chen W,Lin CP,Zhou B,Zhang H

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Fri May 31 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

Molecular mechanisms of IL-33-mediated stromal interactions in cancer metastasis.

JCI insight

Andersson P,Yang Y,Hosaka K,Zhang Y,Fischer C,Braun H,Liu S,Yu G,Liu S,Beyaert R,Chang M,Li Q,Cao Y

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunohistochemistry

Thu Oct 18 00:00:00 EDT 2018

Pulmonary pericytes regulate lung morphogenesis.

Nature communications

Kato K,Diéguez-Hurtado R,Park DY,Hong SP,Kato-Azuma S,Adams S,Stehling M,Trappmann B,Wrana JL,Koh GY,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction.

The Journal of clinical investigation

Nakaya M,Watari K,Tajima M,Nakaya T,Matsuda S,Ohara H,Nishihara H,Yamaguchi H,Hashimoto A,Nishida M,Nagasaka A,Horii Y,Ono H,Iribe G,Inoue R,Tsuda M,Inoue K,Tanaka A,Kuroda M,Nagata S,Kurose H

16140282 was used in immunohistochemistry - frozen section to observe that cardiac myofibroblasts efficiently engulf dead cells

Tue Jan 03 00:00:00 EST 2017

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.

Developmental cell

Naiman N,Fujioka K,Fujino M,Valerius MT,Potter SS,McMahon AP,Kobayashi A

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Mon May 22 00:00:00 EDT 2017

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.

Developmental cell

Naiman N,Fujioka K,Fujino M,Valerius MT,Potter SS,McMahon AP,Kobayashi A

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Mon May 22 00:00:00 EDT 2017

Smooth muscle cell recruitment to lymphatic vessels requires PDGFB and impacts vessel size but not identity.

Development (Cambridge, England)

Wang Y,Jin Y,Mäe MA,Zhang Y,Ortsäter H,Betsholtz C,Mäkinen T,Jakobsson L

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Sun Oct 01 00:00:00 EDT 2017

Neonatal hypothyroidism affects testicular glucose homeostasis through increased oxidative stress in prepubertal mice: effects on GLUT3, GLUT8 and Cx43.

Andrology

Sarkar D,Singh SK

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Sat Jul 01 00:00:00 EDT 2017

Laminin differentially regulates the stemness of type I and type II pericytes.

Stem cell research & therapy

Gautam J,Nirwane A,Yao Y

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Tue Feb 07 00:00:00 EST 2017

Laminin differentially regulates the stemness of type I and type II pericytes.

Stem cell research & therapy

Gautam J,Nirwane A,Yao Y

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Tue Feb 07 00:00:00 EST 2017

Pericytes regulate VEGF-induced endothelial sprouting through VEGFR1.

Nature communications

Eilken HM,Diéguez-Hurtado R,Schmidt I,Nakayama M,Jeong HW,Arf H,Adams S,Ferrara N,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunohistochemistry

Fri Nov 17 00:00:00 EST 2017

Neonatal pancreatic pericytes support β-cell proliferation.

Molecular metabolism

Epshtein A,Rachi E,Sakhneny L,Mizrachi S,Baer D,Landsman L

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Age-dependent modulation of vascular niches for haematopoietic stem cells.

Nature

Kusumbe AP,Ramasamy SK,Itkin T,Mäe MA,Langen UH,Betsholtz C,Lapidot T,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2016

Age-dependent modulation of vascular niches for haematopoietic stem cells.

Nature

Kusumbe AP,Ramasamy SK,Itkin T,Mäe MA,Langen UH,Betsholtz C,Lapidot T,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2016

Age-dependent modulation of vascular niches for haematopoietic stem cells.

Nature

Kusumbe AP,Ramasamy SK,Itkin T,Mäe MA,Langen UH,Betsholtz C,Lapidot T,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2016

Age-dependent modulation of vascular niches for haematopoietic stem cells.

Nature

Kusumbe AP,Ramasamy SK,Itkin T,Mäe MA,Langen UH,Betsholtz C,Lapidot T,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2016

Paracrine Wnt1 Drives Interstitial Fibrosis without Inflammation by Tubulointerstitial Cross-Talk.

Journal of the American Society of Nephrology : JASN

Maarouf OH,Aravamudhan A,Rangarajan D,Kusaba T,Zhang V,Welborn J,Gauvin D,Hou X,Kramann R,Humphreys BD

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Tue Mar 01 00:00:00 EST 2016

Paracrine Wnt1 Drives Interstitial Fibrosis without Inflammation by Tubulointerstitial Cross-Talk.

Journal of the American Society of Nephrology : JASN

Maarouf OH,Aravamudhan A,Rangarajan D,Kusaba T,Zhang V,Welborn J,Gauvin D,Hou X,Kramann R,Humphreys BD

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Tue Mar 01 00:00:00 EST 2016

Epithelial cell integrin β1 is required for developmental angiogenesis in the pituitary gland.

Proceedings of the National Academy of Sciences of the United States of America

Scully KM,Skowronska-Krawczyk D,Krawczyk M,Merkurjev D,Taylor H,Livolsi A,Tollkuhn J,Stan RV,Rosenfeld MG

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunohistochemistry

Tue Nov 22 00:00:00 EST 2016

The role of pericytic laminin in blood brain barrier integrity maintenance.

Scientific reports

Gautam J,Zhang X,Yao Y

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunohistochemistry

Thu Nov 03 00:00:00 EDT 2016

The role of pericytic laminin in blood brain barrier integrity maintenance.

Scientific reports

Gautam J,Zhang X,Yao Y

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunohistochemistry

Thu Nov 03 00:00:00 EDT 2016

Laminin regulates PDGFRβ(+) cell stemness and muscle development.

Nature communications

Yao Y,Norris EH,Mason CE,Strickland S

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Tue May 03 00:00:00 EDT 2016

Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney.

JCI insight

Sato Y,Mii A,Hamazaki Y,Fujita H,Nakata H,Masuda K,Nishiyama S,Shibuya S,Haga H,Ogawa O,Shimizu A,Narumiya S,Kaisho T,Arita M,Yanagisawa M,Miyasaka M,Sharma K,Minato N,Kawamoto H,Yanagita M

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Thu Jul 21 00:00:00 EDT 2016

The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages.

Nature communications

Yang Y,Andersson P,Hosaka K,Zhang Y,Cao R,Iwamoto H,Yang X,Nakamura M,Wang J,Zhuang R,Morikawa H,Xue Y,Braun H,Beyaert R,Samani N,Nakae S,Hams E,Dissing S,Fallon PG,Langer R,Cao Y

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Fri May 06 00:00:00 EDT 2016

The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages.

Nature communications

Yang Y,Andersson P,Hosaka K,Zhang Y,Cao R,Iwamoto H,Yang X,Nakamura M,Wang J,Zhuang R,Morikawa H,Xue Y,Braun H,Beyaert R,Samani N,Nakae S,Hams E,Dissing S,Fallon PG,Langer R,Cao Y

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Fri May 06 00:00:00 EDT 2016

Isoform-Specific Modulation of Inflammation Induced by Adenoviral Mediated Delivery of Platelet-Derived Growth Factors in the Adult Mouse Heart.

PloS one

Gallini R,Huusko J,Ylä-Herttuala S,Betsholtz C,Andrae J

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunohistochemistry

Mon Aug 07 00:00:00 EDT 2017

Analysis of the brain mural cell transcriptome.

Scientific reports

He L,Vanlandewijck M,Raschperger E,Andaloussi Mäe M,Jung B,Lebouvier T,Ando K,Hofmann J,Keller A,Betsholtz C

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

Analysis of the brain mural cell transcriptome.

Scientific reports

He L,Vanlandewijck M,Raschperger E,Andaloussi Mäe M,Jung B,Lebouvier T,Ando K,Hofmann J,Keller A,Betsholtz C

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.

Nature communications

Chen Q,Zhang H,Liu Y,Adams S,Eilken H,Stehling M,Corada M,Dejana E,Zhou B,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Fri Aug 12 00:00:00 EDT 2016

Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.

Nature communications

Chen Q,Zhang H,Liu Y,Adams S,Eilken H,Stehling M,Corada M,Dejana E,Zhou B,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Fri Aug 12 00:00:00 EDT 2016

Overexpression of Heme Oxygenase-1 Prevents Renal Interstitial Inflammation and Fibrosis Induced by Unilateral Ureter Obstruction.

PloS one

Chen X,Wei SY,Li JS,Zhang QF,Wang YX,Zhao SL,Yu J,Wang C,Qin Y,Wei QJ,Lv GX,Li B

16140282 was used in immunohistochemistry - paraffin section to study the effect of heme oxygenase-1 on renal fibrosis using a unilateral ureter obstruction model

Fri Jul 08 00:00:00 EDT 2016

Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle.

The Journal of clinical investigation

Quattrocelli M,Swinnen M,Giacomazzi G,Camps J,Barthélemy I,Ceccarelli G,Caluwé E,Grosemans H,Thorrez L,Pelizzo G,Muijtjens M,Verfaillie CM,Blot S,Janssens S,Sampaolesi M

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

The αvβ1 integrin plays a critical in vivo role in tissue fibrosis.

Science translational medicine

Reed NI,Jo H,Chen C,Tsujino K,Arnold TD,DeGrado WF,Sheppard D

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Wed May 20 00:00:00 EDT 2015

Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Crouch EE,Liu C,Silva-Vargas V,Doetsch F

16-1402 was used in Flow cytometry/Cell sorting to develop a rapid FACS purification strategy to simultaneously isolate primary endothelial cells and pericytes from brain microregions.

Wed Mar 18 00:00:00 EDT 2015

Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Crouch EE,Liu C,Silva-Vargas V,Doetsch F

16-1402 was used in Flow cytometry/Cell sorting to develop a rapid FACS purification strategy to simultaneously isolate primary endothelial cells and pericytes from brain microregions.

Wed Mar 18 00:00:00 EDT 2015

Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Crouch EE,Liu C,Silva-Vargas V,Doetsch F

16-1402 was used in Flow cytometry/Cell sorting to develop a rapid FACS purification strategy to simultaneously isolate primary endothelial cells and pericytes from brain microregions.

Wed Mar 18 00:00:00 EDT 2015

Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases.

The Journal of biological chemistry

Yin H,Frontini MJ,Arpino JM,Nong Z,O'Neil C,Xu Y,Balint B,Ward AD,Chakrabarti S,Ellis CG,Gros R,Pickering JG

Fri Sep 04 00:00:00 EDT 2015

Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases.

The Journal of biological chemistry

Yin H,Frontini MJ,Arpino JM,Nong Z,O'Neil C,Xu Y,Balint B,Ward AD,Chakrabarti S,Ellis CG,Gros R,Pickering JG

Fri Sep 04 00:00:00 EDT 2015

Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases.

The Journal of biological chemistry

Yin H,Frontini MJ,Arpino JM,Nong Z,O'Neil C,Xu Y,Balint B,Ward AD,Chakrabarti S,Ellis CG,Gros R,Pickering JG

Fri Sep 04 00:00:00 EDT 2015

Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases.

The Journal of biological chemistry

Yin H,Frontini MJ,Arpino JM,Nong Z,O'Neil C,Xu Y,Balint B,Ward AD,Chakrabarti S,Ellis CG,Gros R,Pickering JG

Fri Sep 04 00:00:00 EDT 2015

Pericytes are progenitors for coronary artery smooth muscle.

eLife

Volz KS,Jacobs AH,Chen HI,Poduri A,McKay AS,Riordan DP,Kofler N,Kitajewski J,Weissman I,Red-Horse K

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Mon Oct 19 00:00:00 EDT 2015

Pericytes are progenitors for coronary artery smooth muscle.

eLife

Volz KS,Jacobs AH,Chen HI,Poduri A,McKay AS,Riordan DP,Kofler N,Kitajewski J,Weissman I,Red-Horse K

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Mon Oct 19 00:00:00 EDT 2015

Mfsd2a is critical for the formation and function of the blood-brain barrier.

Nature

Ben-Zvi A,Lacoste B,Kur E,Andreone BJ,Mayshar Y,Yan H,Gu C

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Thu May 22 00:00:00 EDT 2014

Formation of persistent hyperplastic primary vitreous in ephrin-A5-/- mice.

Investigative ophthalmology & visual science

Son AI,Sheleg M,Cooper MA,Sun Y,Kleiman NJ,Zhou R

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Wed Mar 19 00:00:00 EDT 2014

Distribution of microsomal prostaglandin E synthase-1 in the mouse brain.

The Journal of comparative neurology

Eskilsson A,Tachikawa M,Hosoya K,Blomqvist A

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Wed Oct 01 00:00:00 EDT 2014

Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity.

Nature communications

Yao Y,Chen ZL,Norris EH,Strickland S

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Mon Mar 03 00:00:00 EST 2014

Oligodendrocyte precursor cells support blood-brain barrier integrity via TGF-β signaling.

PloS one

Seo JH,Maki T,Maeda M,Miyamoto N,Liang AC,Hayakawa K,Pham LD,Suwa F,Taguchi A,Matsuyama T,Ihara M,Kim KW,Lo EH,Arai K

16-1402 was used in Immunohistochemistry to demonstrate that oligodendrocyte precursor cells increase blood-brain barrier tightness by upregulating tight junction proteins via TGF-β signaling.

Thu Nov 05 00:00:00 EST 2015

Comparison of vascular growth factors in the murine brain reveals placenta growth factor as prime candidate for CNS revascularization.

Blood

Gaál EI,Tammela T,Anisimov A,Marbacher S,Honkanen P,Zarkada G,Leppänen VM,Tatlisumak T,Hernesniemi J,Niemelä M,Alitalo K

16-1402 was used in Immunohistochemistry to characterise the effects of endothelium-specific growth factors on the brain vasculature and parenchyma.

Thu Aug 01 00:00:00 EDT 2013

Wnt4/β-catenin signaling in medullary kidney myofibroblasts.

Journal of the American Society of Nephrology : JASN

DiRocco DP,Kobayashi A,Taketo MM,McMahon AP,Humphreys BD

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Sun Sep 01 00:00:00 EDT 2013

Ephrin-B2 controls PDGFRβ internalization and signaling.

Genes & development

Nakayama A,Nakayama M,Turner CJ,Höing S,Lepore JJ,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Sun Dec 01 00:00:00 EST 2013

Ephrin-B2 controls PDGFRβ internalization and signaling.

Genes & development

Nakayama A,Nakayama M,Turner CJ,Höing S,Lepore JJ,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Sun Dec 01 00:00:00 EST 2013

Ephrin-B2 controls PDGFRβ internalization and signaling.

Genes & development

Nakayama A,Nakayama M,Turner CJ,Höing S,Lepore JJ,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Sun Dec 01 00:00:00 EST 2013

Pericytes derived from adipose-derived stem cells protect against retinal vasculopathy.

PloS one

Mendel TA,Clabough EB,Kao DS,Demidova-Rice TN,Durham JT,Zotter BC,Seaman SA,Cronk SM,Rakoczy EP,Katz AJ,Herman IM,Peirce SM,Yates PA

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2014

The bHLH transcription factor Tcf21 is required for lineage-specific EMT of cardiac fibroblast progenitors.

Development (Cambridge, England)

Acharya A,Baek ST,Huang G,Eskiocak B,Goetsch S,Sung CY,Banfi S,Sauer MF,Olsen GS,Duffield JS,Olson EN,Tallquist MD

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Fri Jun 01 00:00:00 EDT 2012

Multiple stromal populations contribute to pulmonary fibrosis without evidence for epithelial to mesenchymal transition.

Proceedings of the National Academy of Sciences of the United States of America

Rock JR,Barkauskas CE,Cronce MJ,Xue Y,Harris JR,Liang J,Noble PW,Hogan BL

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunocytochemistry

Tue Dec 27 00:00:00 EST 2011

Differential contribution to neuroendocrine tumorigenesis of parallel egfr signaling in cancer cells and pericytes.

Genes & cancer

Nolan-Stevaux O,Truitt MC,Pahler JC,Olson P,Guinto C,Lee DC,Hanahan D

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2010

Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development.

Cell stem cell

Kobayashi A,Valerius MT,Mugford JW,Carroll TJ,Self M,Oliver G,McMahon AP

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 16-1402-82) in Immunofluorescence

Thu Aug 07 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b; CD140b antigen; PDGF beta chain; PDGF Receptor beta; PDGF-R-beta; PDGFR-1; PDGFR-beta; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1

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Gene Aliases: AI528809; CD140b; Pdgfr; PDGFR-1; Pdgfr1; Pdgfrb

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UniProt ID: (Mouse) P05622

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Entrez Gene ID: (Mouse) 18596

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity signal transducer activity platelet-derived growth factor-activated receptor activity platelet-derived growth factor beta-receptor activity receptor binding platelet-derived growth factor receptor binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding vascular endothelial growth factor binding phosphatidylinositol 3-kinase binding platelet-derived growth factor binding transmembrane signal receptor

Process(es)

Performance Guarantee

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CD140b (PDGFRB) Monoclonal Antibody (APB5), Functional Grade, eBioscience™

CD140b (PDGFRB) Antibody (16-1402-82) in Flow

Product Details

16-1402-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

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Product Specific Information

Target Information

Bioinformatics

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