Invitrogen

CD20 Monoclonal Antibody (2H7), APC-eFluor™ 780, eBioscience™

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CD20 Antibody (47-0209-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0199-80) and Mouse IgG2b K Isotype Control APC-eFluor® 780 (Product # 47-4732-80) (left) or Anti-Human CD20 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

47-0209-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2H7

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272038

Product Specific Information

Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blod cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Spontaneous EBV-Reactivation during B Cell Differentiation as a Model for Polymorphic EBV-Driven Lymphoproliferation.

Cancers

Care MA,Stephenson S,Owen R,Doody GM,Tooze RM

Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry

Wed Jun 07 00:00:00 EDT 2023

A Soluble NK-CAR Mediates the Specific Cytotoxicity of NK Cells toward the Target CD20⁺ Lymphoma Cells.

Aging and disease

Liu R,Luo Q,Luo W,Wan L,Zhu Q,Yin X,Lu X,Song Z,Wei L,Xiang Z,Zou Y

Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

Inactivated whole-virion vaccine BBV152/Covaxin elicits robust cellular immune memory to SARS-CoV-2 and variants of concern.

Nature microbiology

Vikkurthi R,Ansari A,Pai AR,Jha SN,Sachan S,Pandit S,Nikam B,Kalia A,Jit BP,Parray HA,Singh S,Kshetrapal P,Wadhwa N,Shrivastava T,Coshic P,Kumar S,Sharma P,Sharma N,Taneja J,Pandey AK,Sharma A,Thiruvengadam R,Grifoni A,Weiskopf D,Sette A,Bhatnagar S,Gupta N

47-0209-42 was used in Flow cytometry/Cell sorting to show that inactivated vaccine BBV152 induces robust immune memory to SARS-CoV-2 and variants of concern that persists for at least 6 months after vaccination.

Fri Jul 01 00:00:00 EDT 2022

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

Antigen-Specific Adaptive Immunity to SARS-CoV-2 in Acute COVID-19 and Associations with Age and Disease Severity.

Cell

Rydyznski Moderbacher C,Ramirez SI,Dan JM,Grifoni A,Hastie KM,Weiskopf D,Belanger S,Abbott RK,Kim C,Choi J,Kato Y,Crotty EG,Kim C,Rawlings SA,Mateus J,Tse LPV,Frazier A,Baric R,Peters B,Greenbaum J,Ollmann Saphire E,Smith DM,Sette A,Crotty S

47-0209 was used in Flow cytometry/Cell sorting to complete a combined examination of all three branches of adaptive immunity at the level of SARS-CoV-2-specific CD4+ and CD8+ T cell and neutralizing antibody responses in acute and convalescent subjects.

Thu Nov 12 00:00:00 EST 2020

The Immune Response to Eastern Equine Encephalitis Virus Acquired Through Organ Transplantation.

Frontiers in microbiology

Raabe V,Lai L,Xu Y,Huerta C,Wang D,Pouch SM,Burke CW,Piper AE,Gardner CL,Glass PJ,Mulligan MJ

47-0209 was used in Flow cytometry/Cell sorting to examine the humoral and cellular immune response to EEEV acquired from an infected donor via liver transplantation.

Thu Oct 22 00:00:00 EDT 2020

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

Genetic modification of primary human B cells to model high-grade lymphoma.

Nature communications

Caeser R,Di Re M,Krupka JA,Gao J,Lara-Chica M,Dias JML,Cooke SL,Fenner R,Usheva Z,Runge HFP,Beer PA,Eldaly H,Pak HK,Park CS,Vassiliou GS,Huntly BJP,Mupo A,Bashford-Rogers RJM,Hodson DJ

Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry

Fri Oct 04 00:00:00 EDT 2019

Peripherally derived T regulatory and γδ T cells have opposing roles in the pathogenesis of intractable pediatric epilepsy.

The Journal of experimental medicine

Xu D,Robinson AP,Ishii T,Duncan DS,Alden TD,Goings GE,Ifergan I,Podojil JR,Penaloza-MacMaster P,Kearney JA,Swanson GT,Miller SD,Koh S

47-0209 was used in Flow cytometry/Cell sorting to study the roles of peripherally derived regulatory and γΔ T cells in the pathogenesis of intractable paediatric epilepsy.

Mon Apr 02 00:00:00 EDT 2018

Evidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation.

Nature communications

Colonna L,Peterson CW,Schell JB,Carlson JM,Tkachev V,Brown M,Yu A,Reddy S,Obenza WM,Nelson V,Polacino PS,Mack H,Hu SL,Zeleski K,Hoffman M,Olvera J,Furlan SN,Zheng H,Taraseviciute A,Hunt DJ,Betz K,Lane JF,Vogel K,Hotchkiss CE,Moats C,Baldessari A,Murnane RD,English C,Astley CA,Wangari S,Agricola B,Ahrens J,Iwayama N,May A,Stensland L,Huang MW,Jerome KR,Kiem HP,Kean LS

Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry

Thu Oct 25 00:00:00 EDT 2018

Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.

Nature communications

Somasundaram R,Zhang G,Fukunaga-Kalabis M,Perego M,Krepler C,Xu X,Wagner C,Hristova D,Zhang J,Tian T,Wei Z,Liu Q,Garg K,Griss J,Hards R,Maurer M,Hafner C,Mayerhöfer M,Karanikas G,Jalili A,Bauer-Pohl V,Weihsengruber F,Rappersberger K,Koller J,Lang R,Hudgens C,Chen G,Tetzlaff M,Wu L,Frederick DT,Scolyer RA,Long GV,Damle M,Ellingsworth C,Grinman L,Choi H,Gavin BJ,Dunagin M,Raj A,Scholler N,Gross L,Beqiri M,Bennett K,Watson I,Schaider H,Davies MA,Wargo J,Czerniecki BJ,Schuchter L,Herlyn D,Flaherty K,Herlyn M,Wagner SN

Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2017

Automation of cellular therapy product manufacturing: results of a split validation comparing CD34 selection of peripheral blood stem cell apheresis product with a semi-manual vs. an automatic procedure.

Journal of translational medicine

Hümmer C,Poppe C,Bunos M,Stock B,Wingenfeld E,Huppert V,Stuth J,Reck K,Essl M,Seifried E,Bonig H

47-0209 was used in Flow cytometry/Cell sorting to test the feasiblity of CD34 cell selection using an immunomagnetic "normal scale" selection module on the cell processing automat CliniMACS Prodigy.

Wed Mar 16 00:00:00 EDT 2016

Phase I study of a bispecific ligand-directed toxin targeting CD22 and CD19 (DT2219) for refractory B-cell malignancies.

Clinical cancer research : an official journal of the American Association for Cancer Research

Bachanova V,Frankel AE,Cao Q,Lewis D,Grzywacz B,Verneris MR,Ustun C,Lazaryan A,McClune B,Warlick ED,Kantarjian H,Weisdorf DJ,Miller JS,Vallera DA

Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry

Sun Mar 15 00:00:00 EDT 2015

Automated CD34+ cell isolation of peripheral blood stem cell apheresis product.

Cytotherapy

Spohn G,Wiercinska E,Karpova D,Bunos M,Hümmer C,Wingenfeld E,Sorg N,Poppe C,Huppert V,Stuth J,Reck K,Essl M,Seifried E,Bönig H

47-0209 was used in Flow cytometry/Cell sorting to validate an automatic immunomagnetic cell processing system, CliniMACS Prodigy.

Thu Oct 01 00:00:00 EDT 2015

Characterization of proposed human B-1 cells reveals pre-plasmablast phenotype.

Blood

Covens K,Verbinnen B,Geukens N,Meyts I,Schuit F,Van Lommel L,Jacquemin M,Bossuyt X

47-0209 was used in Flow cytometry/Cell sorting to offer perspectives for the investigation of differentiation of B cells into antibody secreting cells.

Thu Jun 27 00:00:00 EDT 2013

An efficient low cost method for gene transfer to T lymphocytes.

PloS one

Chicaybam L,Sodre AL,Curzio BA,Bonamino MH

Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2013

Transcriptional profiling of mRNAs and microRNAs in human bone marrow precursor B cells identifies subset- and age-specific variations.

PloS one

Jensen K,Brusletto BS,Aass HC,Olstad OK,Kierulf P,Gautvik KM

Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Alterations in peripheral blood B cell subsets and dynamics of B cell responses during human schistosomiasis.

PLoS neglected tropical diseases

Labuda LA,Ateba-Ngoa U,Feugap EN,Heeringa JJ,van der Vlugt LE,Pires RB,Mewono L,Kremsner PG,van Zelm MC,Adegnika AA,Yazdanbakhsh M,Smits HH

47-0209 was used in Flow cytometry/Cell sorting to characterise the B cell response to S. haematobium infection.

Wed Aug 28 00:00:00 EDT 2013

Schistosomes induce regulatory features in human and mouse CD1d(hi) B cells: inhibition of allergic inflammation by IL-10 and regulatory T cells.

PloS one

van der Vlugt LE,Labuda LA,Ozir-Fazalalikhan A,Lievers E,Gloudemans AK,Liu KY,Barr TA,Sparwasser T,Boon L,Ngoa UA,Feugap EN,Adegnika AA,Kremsner PG,Gray D,Yazdanbakhsh M,Smits HH

47-0209 was used in Flow cytometry/Cell sorting to investigate the effect of Schistosoma mansoni infection on allergic airway inflammation, showing it was inhibited by IL-10 produced by CD1d(hi) B cells.

Tue Jul 31 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MGC3969

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Gene Aliases: B1; Bp35; CD20; CVID5; LEU-16; MS4A1; MS4A2; S7

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UniProt ID: (Human) P11836

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Entrez Gene ID: (Human) 931

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Function(s)

epidermal growth factor receptor binding protein binding MHC class II protein complex binding transporter

Process(es)

humoral immune response B cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD20 Monoclonal Antibody (2H7), APC-eFluor™ 780, eBioscience™

CD20 Antibody (47-0209-42) in Flow

Product Details

47-0209-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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