Invitrogen

CD20 Monoclonal Antibody (2H7), eFluor™ 450, eBioscience™

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Antibody Testing Data (1)
Published Figures (15)

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CD20 Antibody (48-0209-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Mouse IgG2b K Isotype Control eFluor® 450 (Product # 48-4732-82) (left) or Anti-Human CD20 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

CD20 Antibody in Immunohistochemistry (IHC)

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Product Details

48-0209-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2H7

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1633384

Product Specific Information

Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Spontaneous EBV-Reactivation during B Cell Differentiation as a Model for Polymorphic EBV-Driven Lymphoproliferation.

Cancers

Care MA,Stephenson S,Owen R,Doody GM,Tooze RM

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry

Wed Jun 07 00:00:00 EDT 2023

A Soluble NK-CAR Mediates the Specific Cytotoxicity of NK Cells toward the Target CD20⁺ Lymphoma Cells.

Aging and disease

Liu R,Luo Q,Luo W,Wan L,Zhu Q,Yin X,Lu X,Song Z,Wei L,Xiang Z,Zou Y

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

γ9δ2 T-Cell Expansion and Phenotypic Profile Are Reflected in the CDR3δ Repertoire of Healthy Adults.

Frontiers in immunology

Vyborova A,Janssen A,Gatti L,Karaiskaki F,Yonika A,van Dooremalen S,Sanders J,Beringer DX,Straetemans T,Sebestyen Z,Kuball J

48-0209-42 was used in Flow cytometry/Cell sorting to establish consistent repertoire-phenotype associations and justifies stratification for the T-cell phenotype in future research on γ9δ2TCR repertoire dynamics.

Tue Jul 26 00:00:00 EDT 2022

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

IL-33 Alarmin and Its Active Proinflammatory Fragments Are Released in Small Intestine in Celiac Disease.

Frontiers in immunology

Perez F,Ruera CN,Miculan E,Carasi P,Dubois-Camacho K,Garbi L,Guzman L,Hermoso MA,Chirdo FG

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Immunohistochemistry

Tue Jun 22 00:00:00 EDT 2021

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

Genetic modification of primary human B cells to model high-grade lymphoma.

Nature communications

Caeser R,Di Re M,Krupka JA,Gao J,Lara-Chica M,Dias JML,Cooke SL,Fenner R,Usheva Z,Runge HFP,Beer PA,Eldaly H,Pak HK,Park CS,Vassiliou GS,Huntly BJP,Mupo A,Bashford-Rogers RJM,Hodson DJ

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry

Fri Oct 04 00:00:00 EDT 2019

CLEC10A Is a Specific Marker for Human CD1c⁺ Dendritic Cells and Enhances Their Toll-Like Receptor 7/8-Induced Cytokine Secretion.

Frontiers in immunology

Heger L,Balk S,Lühr JJ,Heidkamp GF,Lehmann CHK,Hatscher L,Purbojo A,Hartmann A,Garcia-Martin F,Nishimura SI,Cesnjevar R,Nimmerjahn F,Dudziak D

48-0209 was used in Flow cytometry/Cell sorting to identify CLEC10A as a specific marker for human CD1c+ dendritic cells and that this rapidly internalises into human CD1c dendritic cells upon binding of a monoclonal antibody directed against CLEC10A.

Thu Jun 13 00:00:00 EDT 2019

Evidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation.

Nature communications

Colonna L,Peterson CW,Schell JB,Carlson JM,Tkachev V,Brown M,Yu A,Reddy S,Obenza WM,Nelson V,Polacino PS,Mack H,Hu SL,Zeleski K,Hoffman M,Olvera J,Furlan SN,Zheng H,Taraseviciute A,Hunt DJ,Betz K,Lane JF,Vogel K,Hotchkiss CE,Moats C,Baldessari A,Murnane RD,English C,Astley CA,Wangari S,Agricola B,Ahrens J,Iwayama N,May A,Stensland L,Huang MW,Jerome KR,Kiem HP,Kean LS

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry

Thu Oct 25 00:00:00 EDT 2018

Early Emergence of CD19-Negative Human Antibody-Secreting Cells at the Plasmablast to Plasma Cell Transition.

Journal of immunology (Baltimore, Md. : 1950)

Arumugakani G,Stephenson SJ,Newton DJ,Rawstron A,Emery P,Doody GM,McGonagle D,Tooze RM

48-0209 was used in Flow cytometry/Cell sorting to investigate whether human CD19-negative plasma cells are aged cells that have gradually lost CD19 expression.

Thu Jun 15 00:00:00 EDT 2017

Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.

Nature communications

Somasundaram R,Zhang G,Fukunaga-Kalabis M,Perego M,Krepler C,Xu X,Wagner C,Hristova D,Zhang J,Tian T,Wei Z,Liu Q,Garg K,Griss J,Hards R,Maurer M,Hafner C,Mayerhöfer M,Karanikas G,Jalili A,Bauer-Pohl V,Weihsengruber F,Rappersberger K,Koller J,Lang R,Hudgens C,Chen G,Tetzlaff M,Wu L,Frederick DT,Scolyer RA,Long GV,Damle M,Ellingsworth C,Grinman L,Choi H,Gavin BJ,Dunagin M,Raj A,Scholler N,Gross L,Beqiri M,Bennett K,Watson I,Schaider H,Davies MA,Wargo J,Czerniecki BJ,Schuchter L,Herlyn D,Flaherty K,Herlyn M,Wagner SN

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Immunohistochemistry

Tue Sep 19 00:00:00 EDT 2017

Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.

Nature communications

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Immunohistochemistry

Tue Sep 19 00:00:00 EDT 2017

Phase I study of a bispecific ligand-directed toxin targeting CD22 and CD19 (DT2219) for refractory B-cell malignancies.

Clinical cancer research : an official journal of the American Association for Cancer Research

Bachanova V,Frankel AE,Cao Q,Lewis D,Grzywacz B,Verneris MR,Ustun C,Lazaryan A,McClune B,Warlick ED,Kantarjian H,Weisdorf DJ,Miller JS,Vallera DA

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry

Sun Mar 15 00:00:00 EDT 2015

Sexual Differences in Cell Loss during the Post-Hatch Development of Song Control Nuclei in the Bengalese Finch.

PloS one

Chen X,Li J,Zeng L,Zhang X,Lu X,Zuo M,Zhang X,Zeng S

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Immunohistochemistry

Mon Apr 04 00:00:00 EDT 2016

Validation and implementation of a method for microarray gene expression profiling of minor B-cell subpopulations in man.

BMC immunology

Bergkvist KS,Nyegaard M,Bøgsted M,Schmitz A,Bødker JS,Rasmussen SM,Perez-Andres M,Falgreen S,Bilgrau AE,Kjeldsen MK,Gaihede M,Nørgaard MA,Bæch J,Grønholdt ML,Jensen FS,Johansen P,Dybkær K,Johnsen HE

48-0209 was used in Flow cytometry/Cell sorting to describe a method for the generation of global gene expression profiles from low frequency B-cell subsets.

Fri Jan 31 00:00:00 EST 2014

An efficient low cost method for gene transfer to T lymphocytes.

PloS one

Chicaybam L,Sodre AL,Curzio BA,Bonamino MH

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2013

Transcriptional profiling of mRNAs and microRNAs in human bone marrow precursor B cells identifies subset- and age-specific variations.

PloS one

Jensen K,Brusletto BS,Aass HC,Olstad OK,Kierulf P,Gautvik KM

Published figure using CD20 monoclonal antibody (Product # 48-0209-42) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

In vitro generation of long-lived human plasma cells.

Journal of immunology (Baltimore, Md. : 1950)

Cocco M,Stephenson S,Care MA,Newton D,Barnes NA,Davison A,Rawstron A,Westhead DR,Doody GM,Tooze RM

48-0209 was used in Flow cytometry/Cell sorting to develop a model for the generation of polyclonal long-lived human plasma cells from peripheral blood B cells.

Sat Dec 15 00:00:00 EST 2012

Experimental inoculation of juvenile rhesus macaques with primate enteric caliciviruses.

PloS one

Sestak K,Feely S,Fey B,Dufour J,Hargitt E,Alvarez X,Pahar B,Gregoricus N,Vinjé J,Farkas T

48-0209 was used in Flow cytometry/Cell sorting to show that ReCVs represent an alternative cell culture and animal model to study enteric calicivirus replication, pathogenesis and immunity.

Mon Oct 29 00:00:00 EDT 2012

Alterations in peripheral blood B-cell populations in SHIV89.6P-infected macaques (Macacca fascicularis).

Comparative medicine

Kling HM,Shipley TW,Norris KA

48-0209 was used in Flow cytometry/Cell sorting to study how SHIV89.6P infection can alter the peripheral blood B cell population in macaques.

Wed Jun 01 00:00:00 EDT 2011

Alterations in peripheral blood B-cell populations in SHIV89.6P-infected macaques (Macacca fascicularis).

Comparative medicine

Kling HM,Shipley TW,Norris KA

48-0209 was used in Flow cytometry/Cell sorting to study how SHIV89.6P infection can alter the peripheral blood B cell population in macaques.

Wed Jun 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MGC3969

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Gene Aliases: B1; Bp35; CD20; CVID5; LEU-16; MS4A1; MS4A2; S7

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UniProt ID: (Human) P11836

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Entrez Gene ID: (Human) 931

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Function(s)

epidermal growth factor receptor binding protein binding MHC class II protein complex binding transporter

Process(es)

humoral immune response B cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD20 Monoclonal Antibody (2H7), eFluor™ 450, eBioscience™

CD20 Antibody (48-0209-42) in Flow

Product Details

48-0209-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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