Invitrogen

CD27 Monoclonal Antibody (LG.7F9), eBioscience™

FIGURE: 1 / 21

CD27 Antibody (14-0271-82) in Flow

Staining of mouse splenocytes with 0.25 µg Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg Anti-CD27 Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111). Total viable cells were used for analysis.

Product Image Gallery

Product Details

14-0271-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 52 publications 52 publications

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

Assay-Dependent

Functional Assay (Functional)

Assay-Dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

LG.7F9

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467183

Product Specific Information

Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported: The LG.7F9 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. Use of mild detergent lysates is recommended when performing immunoprecipitation with this mAb. LG.7F9 has also been reported in blocking of ligand binding. (Please use Functional Grade purified LG.7F9, Product # 16-0271, in functional assays).

Applications Tested: The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Mon Oct 03 00:00:00 EDT 2022

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Effect of Subcutaneous Anti-CD20 Antibody-Mediated B Cell Depletion on Susceptibility to Pneumocystis Infection in Mice.

mSphere

Dai G,Noell K,Weckbecker G,Kolls JK

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Wed May 05 00:00:00 EDT 2021

Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.

Cell reports. Medicine

Rouers A,Chng MHY,Lee B,Rajapakse MP,Kaur K,Toh YX,Sathiakumar D,Loy T,Thein TL,Lim VWX,Singhal A,Yeo TW,Leo YS,Vora KA,Casimiro D,Lim B,Tucker-Kellogg L,Rivino L,Newell EW,Fink K

14-0271-82 was used in Flow Cytometry to reveal associations between cellular profiles and disease severity, opening opportunities to study immunopathology in dengue disease and the potential predictive value of these parameters.

Tue May 18 00:00:00 EDT 2021

Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.

Cell reports. Medicine

Rouers A,Chng MHY,Lee B,Rajapakse MP,Kaur K,Toh YX,Sathiakumar D,Loy T,Thein TL,Lim VWX,Singhal A,Yeo TW,Leo YS,Vora KA,Casimiro D,Lim B,Tucker-Kellogg L,Rivino L,Newell EW,Fink K

Tue May 18 00:00:00 EDT 2021

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.

Cell reports

Seydoux E,Wan YH,Feng J,Wall A,Aljedani S,Homad LJ,MacCamy AJ,Weidle C,Gray MD,Brumage L,Taylor JJ,Pancera M,Stamatatos L,McGuire AT

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

Ontogenic timing, T cell receptor signal strength, and Notch signaling direct γδ T cell functional differentiation in vivo.

Cell reports

Chen ELY,Lee CR,Thompson PK,Wiest DL,Anderson MK,Zúñiga-Pflücker JC

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Coordinated Viral Control by Cytotoxic Lymphocytes Ensures Optimal Adaptive NK Cell Responses.

Cell reports

Diaz-Salazar C,Sun JC

14-0271 was used in Flow cytometry/Cell sorting to support a mechanism whereby cytotoxic innate and adaptive lymphocytes cooperate to ensure viral clearance and the establishment of robust clonal NK cell responses.

Tue Sep 22 00:00:00 EDT 2020

Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells.

Cell reports

Vink CS,Calero-Nieto FJ,Wang X,Maglitto A,Mariani SA,Jawaid W,Göttgens B,Dzierzak E

Tue May 12 00:00:00 EDT 2020

Divergent Role for STAT5 in the Adaptive Responses of Natural Killer Cells.

Cell reports

Wiedemann GM,Grassmann S,Lau CM,Rapp M,Villarino AV,Friedrich C,Gasteiger G,O'Shea JJ,Sun JC

14-0271 was used in Flow cytometry/Cell sorting to investigate how STAT5 regulates transcription during viral infection.

Tue Dec 15 00:00:00 EST 2020

Cytomegalovirus Infection Drives Avidity Selection of Natural Killer Cells.

Immunity

Adams NM,Geary CD,Santosa EK,Lumaquin D,Le Luduec JB,Sottile R,van der Ploeg K,Hsu J,Whitlock BM,Jackson BT,Weizman OE,Huse M,Hsu KC,Sun JC

14-0271 was used in Flow cytometry/Cell sorting to delineate how heterogeneity in NK cell avidity diversifies NK cell effector function during antiviral immunity, and how avidity selection might serve to produce the most potent memory NK cells.

Tue Jun 18 00:00:00 EDT 2019

The complex of MCMV proteins and MHC class I evades NK cell control and drives the evolution of virus-specific activating Ly49 receptors.

The Journal of experimental medicine

Železnjak J,Lisnić VJ,Popović B,Lisnić B,Babić M,Halenius A,L'Hernault A,Roviš TL,Hengel H,Erhard F,Redwood AJ,Vidal SM,Dölken L,Krmpotić A,Jonjić S

14-0271 was used in Flow cytometry/Cell sorting to solve a long-standing conundrum of how MCMV avoids recognition by NK cells, unravels a fundamental new viral immune evasion mechanism, and demonstrates how this forced the evolution of virus-specific activating MHC I-restricted Ly49 receptors.

Mon Aug 05 00:00:00 EDT 2019

Continuous activity of Foxo1 is required to prevent anergy and maintain the memory state of CD8⁺ T cells.

The Journal of experimental medicine

Delpoux A,Michelini RH,Verma S,Lai CY,Omilusik KD,Utzschneider DT,Redwood AJ,Goldrath AW,Benedict CA,Hedrick SM

14-0271 was used in Flow cytometry/Cell sorting to show that FOXO1 is continuously required for all the phenotypic characteristics of memory-effector T cells.

Mon Feb 05 00:00:00 EST 2018

Tumor-associated neutrophils suppress pro-tumoral IL-17+ γδ T cells through induction of oxidative stress.

PLoS biology

Mensurado S,Rei M,Lança T,Ioannou M,Gonçalves-Sousa N,Kubo H,Malissen M,Papayannopoulos V,Serre K,Silva-Santos B

14-0271 was used in Flow cytometry/Cell sorting to study the role of tumour-associated neutrophils in the regulation of γδ17 T-cell responses in the tumour microenvironment.

Tue May 01 00:00:00 EDT 2018

BALDR: a computational pipeline for paired heavy and light chain immunoglobulin reconstruction in single-cell RNA-seq data.

Genome medicine

Upadhyay AA,Kauffman RC,Wolabaugh AN,Cho A,Patel NB,Reiss SM,Havenar-Daughton C,Dawoud RA,Tharp GK,Sanz I,Pulendran B,Crotty S,Lee FE,Wrammert J,Bosinger SE

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.

Nature communications

Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Mon Nov 19 00:00:00 EST 2018

Non-redundant ISGF3 Components Promote NK Cell Survival in an Auto-regulatory Manner during Viral Infection.

Cell reports

Geary CD,Krishna C,Lau CM,Adams NM,Gearty SV,Pritykin Y,Thomsen AR,Leslie CS,Sun JC

Tue Aug 21 00:00:00 EDT 2018

TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival.

Nature communications

Zhu L,Xie X,Zhang L,Wang H,Jie Z,Zhou X,Shi J,Zhao S,Zhang B,Cheng X,Sun SC

14-0271 was used in Flow cytometry/Cell sorting to study the mechanism of IL-15 function in autophagy and natural killer cell survival.

Wed Jul 18 00:00:00 EDT 2018

Casein kinase 2 controls the survival of normal thymic and leukemic γδ T cells via promotion of AKT signaling.

Leukemia

Ribeiro ST,Tesio M,Ribot JC,Macintyre E,Barata JT,Silva-Santos B

14027182 was used in flow cytometry to identify a role for CK2 in healthy human thymocytes in the selection of the gamma delta T-cell lineage

Sat Jul 01 00:00:00 EDT 2017

NFκB-Pim-1-Eomesodermin axis is critical for maintaining CD8 T-cell memory quality.

Proceedings of the National Academy of Sciences of the United States of America

Knudson KM,Pritzl CJ,Saxena V,Altman A,Daniels MA,Teixeiro E

14-0271 was used in Flow cytometry/Cell sorting to show that TCR/antigenic signals regulate the induction of a NFκB/Pim-1 feedback loop that controls expression of Eomes and thereby long-term maintenance of the memory pool.

Tue Feb 28 00:00:00 EST 2017

Polymicrobial sepsis impairs bystander recruitment of effector cells to infected skin despite optimal sensing and alarming function of skin resident memory CD8 T cells.

PLoS pathogens

Danahy DB,Anthony SM,Jensen IJ,Hartwig SM,Shan Q,Xue HH,Harty JT,Griffith TS,Badovinac VP

14-0271-82 was used in Flow Cytometry to directly examine the impact of sepsis on tissue resident memory CD8+ T cells in mice.

Fri Sep 01 00:00:00 EDT 2017

NCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation.

European journal of immunology

Miletic A,Lenartic M,Popovic B,Brizic I,Trsan T,Miklic K,Mandelboim O,Krmpotic A,Jonjic S

14-0271 was used in Flow cytometry/Cell sorting to provide evidence for the role of NCR1 in antibody response to mouse cytomegalovirus infection.

Fri Sep 01 00:00:00 EDT 2017

NK cells regulate CXCR2+ neutrophil recruitment during acute lung injury.

Journal of leukocyte biology

Hoegl S,Ehrentraut H,Brodsky KS,Victorino F,Golden-Mason L,Eltzschig HK,McNamee EN

14027182 was used in flow cytometry to elucidate the role of Tbet in acute lung injury

Wed Feb 01 00:00:00 EST 2017

NKG2C/E Marks the Unique Cytotoxic CD4 T Cell Subset, ThCTL, Generated by Influenza Infection.

Journal of immunology (Baltimore, Md. : 1950)

Marshall NB,Vong AM,Devarajan P,Brauner MD,Kuang Y,Nayar R,Schutten EA,Castonguay CH,Berg LJ,Nutt SL,Swain SL

14027182 was used in flow cytometry to determine that NKG2C/E identifies the CD4 T cell effector subset ThCTL that develop in the lung during influenza A virus infection in mice

Wed Feb 01 00:00:00 EST 2017

WASH maintains NKp46⁺ ILC3 cells by promoting AHR expression.

Nature communications

Xia P,Liu J,Wang S,Ye B,Du Y,Xiong Z,Han ZG,Tong L,Fan Z

14-0271 was used in Flow cytometry/Cell sorting to study how WASH-mediated AHR expression has a critical function in the maintenance of NKp46(+) ILC3s.

Wed Jun 07 00:00:00 EDT 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Differential Responsiveness of Innate-like IL-17- and IFN-γ-Producing γδ T Cells to Homeostatic Cytokines.

Journal of immunology (Baltimore, Md. : 1950)

Corpuz TM,Stolp J,Kim HO,Pinget GV,Gray DH,Cho JH,Sprent J,Webster KE

14-0271 was used in Flow cytometry/Cell sorting to suggest that γδ T cell subsets do not directly compete with each other for cytokines, but instead fall into resource niches with other functionally similar lymphocytes.

Fri Jan 15 00:00:00 EST 2016

NK Cell Maturation and Cytotoxicity Are Controlled by the Intramembrane Aspartyl Protease SPPL3.

Journal of immunology (Baltimore, Md. : 1950)

Hamblet CE,Makowski SL,Tritapoe JM,Pomerantz JL

14-0271 was used in Flow cytometry/Cell sorting to investigate the role of SPPL3 in murine NK cell biology.

Tue Mar 15 00:00:00 EDT 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Wed Sep 07 00:00:00 EDT 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

Published figure using CD27 monoclonal antibody (Product # 14-0271-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

Transcriptional repressor ZEB2 promotes terminal differentiation of CD8+ effector and memory T cell populations during infection.

The Journal of experimental medicine

Omilusik KD,Best JA,Yu B,Goossens S,Weidemann A,Nguyen JV,Seuntjens E,Stryjewska A,Zweier C,Roychoudhuri R,Gattinoni L,Bird LM,Higashi Y,Kondoh H,Huylebroeck D,Haigh J,Goldrath AW

14-0271-82 was used in Flow Cytometry to study the upregulation of ZEB2 by activated T cells, specifically in the KLRG1(hi) effector CD8(+) T cell subset.

Mon Nov 16 00:00:00 EST 2015

OX40- and CD27-mediated costimulation synergizes with anti-PD-L1 blockade by forcing exhausted CD8+ T cells to exit quiescence.

Journal of immunology (Baltimore, Md. : 1950)

Buchan S,Manzo T,Flutter B,Rogel A,Edwards N,Zhang L,Sivakumaran S,Ghorashian S,Carpenter B,Bennett C,Freeman GJ,Sykes M,Croft M,Al-Shamkhani A,Chakraverty R

14-0271 was used in Flow cytometry/Cell sorting to study how CD27-mediated co-stimulation can synergise with co-inhibitory checkpoint blockade to switch off molecular programs for quiescence in exhausted T cell populations.

Thu Jan 01 00:00:00 EST 2015

PI3Kδ Regulates the Magnitude of CD8+ T Cell Responses after Challenge with Listeria monocytogenes.

Journal of immunology (Baltimore, Md. : 1950)

Pearce VQ,Bouabe H,MacQueen AR,Carbonaro V,Okkenhaug K

14-0271 was used in Flow cytometry/Cell sorting to investigate the effect of inhibition of the p110δ subunit of PI3Kδ on CD8(+) T cell responses to Listeria monocytogenes infection.

Thu Oct 01 00:00:00 EDT 2015

The transcription factor E4bp4/Nfil3 controls commitment to the NK lineage and directly regulates Eomes and Id2 expression.

The Journal of experimental medicine

Male V,Nisoli I,Kostrzewski T,Allan DS,Carlyle JR,Lord GM,Wack A,Brady HJ

14-0271 was used in Flow cytometry/Cell sorting to propose that E4bp4 is required for commitment to the NK lineage and can promote NK development by directly regulating the expression of the downstream transcription factors Eomes and Id2.

Mon Apr 07 00:00:00 EDT 2014

Murine CD27(-) Vγ6(+) γδ T cells producing IL-17A promote ovarian cancer growth via mobilization of protumor small peritoneal macrophages.

Proceedings of the National Academy of Sciences of the United States of America

Rei M,Gonçalves-Sousa N,Lança T,Thompson RG,Mensurado S,Balkwill FR,Kulbe H,Pennington DJ,Silva-Santos B

14-0271 was used in Flow cytometry/Cell sorting to characterise the role of tumour-associated γδ T cells on disease development using a model peritoneal/ovarian cancer cell line.

Tue Aug 26 00:00:00 EDT 2014

Ly49C-dependent control of MCMV Infection by NK cells is cis-regulated by MHC Class I molecules.

PLoS pathogens

Forbes CA,Scalzo AA,Degli-Esposti MA,Coudert JD

14-0271 was used in Flow cytometry/Cell sorting to provide a novel insight into the mechanisms that regulate NK cell activation during viral infection.

Thu May 01 00:00:00 EDT 2014

Microsphere priming facilitates induction of potent therapeutic T-cell immune responses against autochthonous liver cancers.

European journal of immunology

Brinkhoff B,Ostroumov D,Heemcke J,Woller N,Gürlevik E,Manns MP,Longerich T,Zender L,Harty JT,Kubicka S,Kühnel F,Wirth TC

14-0271-82 was used in Flow Cytometry to develop a prime-boost vaccination protocol based on the administration of antigen conjugated to poly-lactic-co-glycolic acid microspheres followed by booster vaccination with Listeria monocytogenes vectors.

Tue Apr 01 00:00:00 EDT 2014

Cutaneous immunosurveillance and regulation of inflammation by group 2 innate lymphoid cells.

Nature immunology

Roediger B,Kyle R,Yip KH,Sumaria N,Guy TV,Kim BS,Mitchell AJ,Tay SS,Jain R,Forbes-Blom E,Chen X,Tong PL,Bolton HA,Artis D,Paul WE,Fazekas de St Groth B,Grimbaldeston MA,Le Gros G,Weninger W

14-0271 was used in Flow cytometry/Cell sorting to report the identification in normal mouse dermis of an abundant, phenotypically unique group 2 innate lymphoid cell subset that depended on interleukin 7 and constitutively produced IL-13.

Sat Jun 01 00:00:00 EDT 2013

Low-affinity T cells are programmed to maintain normal primary responses but are impaired in their recall to low-affinity ligands.

Cell reports

Knudson KM,Goplen NP,Cunningham CA,Daniels MA,Teixeiro E

14-0271 was used in Flow cytometry/Cell sorting to demonstrate that distinct mechanisms drive effector/memory development in high- and low-affinity T-cells.

Thu Aug 15 00:00:00 EDT 2013

Population dynamics of naive and memory CD8 T cell responses after antigen stimulations in vivo.

Journal of immunology (Baltimore, Md. : 1950)

Martin MD,Condotta SA,Harty JT,Badovinac VP

14-0271 was used in Flow cytometry/Cell sorting to investigate cell-autonomous functional differences between naive and memory CD8 T cells.

Wed Feb 01 00:00:00 EST 2012

CCR2 defines a distinct population of NK cells and mediates their migration during influenza virus infection in mice.

PloS one

van Helden MJ,Zaiss DM,Sijts AJ

14-0271 was used in Flow cytometry/Cell sorting to determine the role of CCR2 in NK cell migration during intranasal influenza virus infection, showing that it defines a distinct population of NK cells and mediates their migration.

Mon Jun 10 00:00:00 EDT 2013

Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi.

Infection and immunity

Rigato PO,de Alencar BC,de Vasconcelos JR,Dominguez MR,Araújo AF,Machado AV,Gazzinelli RT,Bruna-Romero O,Rodrigues MM

14-0271 was used in Flow cytometry/Cell sorting to demonstrate that heterologous plasmid DNA prime-adenovirus boost vaccination generates a stable pool of CD8(+) T effector memory cells.

Sun May 01 00:00:00 EDT 2011

Extreme CD8 T cell requirements for anti-malarial liver-stage immunity following immunization with radiation attenuated sporozoites.

PLoS pathogens

Schmidt NW,Butler NS,Badovinac VP,Harty JT

14-0271 was used in Flow cytometry/Cell sorting to identify and track whole-parasite, RAS-vaccine-induced effector and memory CD8 T cell responses.

Thu Jul 15 00:00:00 EDT 2010

Cutting edge: adaptive versus innate receptor signals selectively control the pool sizes of murine IFN-γ- or IL-17-producing γδ T cells upon infection.

Journal of immunology (Baltimore, Md. : 1950)

Ribot JC,Chaves-Ferreira M,d'Orey F,Wencker M,Gonçalves-Sousa N,Decalf J,Simas JP,Hayday AC,Silva-Santos B

14-0271 was used in Flow cytometry/Cell sorting to investigate the properties of γδ T lymphocytes for adaptive and innate immunity.

Wed Dec 01 00:00:00 EST 2010

Type I IFN signaling constrains IL-17A/F secretion by gammadelta T cells during bacterial infections.

Journal of immunology (Baltimore, Md. : 1950)

Henry T,Kirimanjeswara GS,Ruby T,Jones JW,Peng K,Perret M,Ho L,Sauer JD,Iwakura Y,Metzger DW,Monack DM

14-0271 was used in Flow cytometry/Cell sorting to determine that type I IFN signalling might contribute to the resistance of mice to infection with other intracellular bacteria.

Thu Apr 01 00:00:00 EDT 2010

GITR triggering induces expansion of both effector and regulatory CD4+ T cells in vivo.

Journal of immunology (Baltimore, Md. : 1950)

van Olffen RW,Koning N,van Gisbergen KP,Wensveen FM,Hoek RM,Boon L,Hamann J,van Lier RA,Nolte MA

14-0271 was used in Flow cytometry/Cell sorting to study the role of glucocorticoid-induced TNF receptor family-related protein on activated and regulatory T cells.

Mon Jun 15 00:00:00 EDT 2009

In vivo expression of signaling proteins in reconstituted NK cells.

Journal of immunological methods

Orr SJ,Quigley L,McVicar DW

14-0271 was used in Flow cytometry/Cell sorting to develop a novel technique to generate NK cells in vivo, all of which express a gene of interest.

Fri Jan 30 00:00:00 EST 2009

Interleukin 15-mediated survival of natural killer cells is determined by interactions among Bim, Noxa and Mcl-1.

Nature immunology

Huntington ND,Puthalakath H,Gunn P,Naik E,Michalak EM,Smyth MJ,Tabarias H,Degli-Esposti MA,Dewson G,Willis SN,Motoyama N,Huang DC,Nutt SL,Tarlinton DM,Strasser A

14-0271 was used in Flow cytometry/Cell sorting to identify Bim, Noxa and Mcl-1 as key regulators of IL-15-dependent survival of NK cells.

Wed Aug 01 00:00:00 EDT 2007

The impact of a boosting immunogen on the differentiation of secondary memory CD8+ T cells.

Journal of virology

Hovav AH,Panas MW,Osuna CE,Cayabyab MJ,Autissier P,Letvin NL

14-0271 was used in Flow cytometry/Cell sorting to evaluate the effect of three different immunogens on the generation of memory cellular immune responses.

Sat Dec 01 00:00:00 EST 2007

Duration of antigen expression in vivo following DNA immunization modifies the magnitude, contraction, and secondary responses of CD8+ T lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Hovav AH,Panas MW,Rahman S,Sircar P,Gillard G,Cayabyab MJ,Letvin NL

14-0271 was used in Flow cytometry/Cell sorting to investigate the duration of Ag expression on pathogen-induced CD8(+) T cell response.

Thu Nov 15 00:00:00 EST 2007

Characterization of murine CD70, the ligand of the TNF receptor family member CD27.

Journal of immunology (Baltimore, Md. : 1950)

Tesselaar K,Gravestein LA,van Schijndel GM,Borst J,van Lier RA

Sat Nov 15 00:00:00 EST 1997

Bioinformatics

Protein Aliases: CD antigen 27; CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842, (Mouse) P41272

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Entrez Gene ID: (Human) 939, (Mouse) 21940, (Rat) 500318

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Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process molecular_function

Process(es)

inflammatory response immune response cell surface receptor signaling pathway brain development release of cytoplasmic sequestered NF-kappaB extrinsic apoptotic signaling pathway via death domain receptors immunoglobulin mediated immune response response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to ethanol positive regulation of B cell differentiation positive regulation of JNK cascade negative regulation of T cell apoptotic process extrinsic apoptotic signaling pathway apoptotic process B cell proliferation positive regulation of interferon-gamma biosynthetic process positive regulation of T cell differentiation immunoglobulin secretion

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CD27 Monoclonal Antibody (LG.7F9), eBioscience™

CD27 Antibody (14-0271-82) in Flow

Product Details

14-0271-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

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Bioinformatics

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