Invitrogen

CD27 Monoclonal Antibody (O323), FITC, eBioscience™

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Antibody Testing Data (1)
Published Figures (17)

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CD27 Antibody (11-0279-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD27 FITC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

11-0279-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

O323

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10669045

Product Specific Information

Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.

Applications Reported: The O323 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses.

Life science alliance

Verhoeven D,Grinwis L,Marsman C,Jansen MH,Van Leeuwen EM,Kuijpers TW

Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Oxygen level is a critical regulator of human B cell differentiation and IgG class switch recombination.

Frontiers in immunology

Koers J,Marsman C,Steuten J,Tol S,Derksen NIL,Ten Brinke A,van Ham SM,Rispens T

Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry

Tue Nov 17 00:00:00 EST 2020

More than one antibody of individual B cells revealed by single-cell immune profiling.

Cell discovery

Shi Z,Zhang Q,Yan H,Yang Y,Wang P,Zhang Y,Deng Z,Yu M,Zhou W,Wang Q,Yang X,Mo X,Zhang C,Huang J,Dai H,Sun B,Zhao Y,Zhang L,Yang YG,Qiu X

11-0279-42 was used in Flow Cytometry to reveal an unprecedented presence of multi-Ig specificity in some single B cells, implying regulation of Ig gene rearrangement and class switching that differs from the classical mechanisms of both the "one cell - one antibody" rule and CSR.

Fri Oct 20 00:00:00 EDT 2023

More than one antibody of individual B cells revealed by single-cell immune profiling.

Cell discovery

Shi Z,Zhang Q,Yan H,Yang Y,Wang P,Zhang Y,Deng Z,Yu M,Zhou W,Wang Q,Yang X,Mo X,Zhang C,Huang J,Dai H,Sun B,Zhao Y,Zhang L,Yang YG,Qiu X

Fri Oct 20 00:00:00 EDT 2023

Perturbed CD8⁺ T cell immunity across universal influenza epitopes in the elderly.

Journal of leukocyte biology

Nguyen THO,Sant S,Bird NL,Grant EJ,Clemens EB,Koutsakos M,Valkenburg SA,Gras S,Lappas M,Jaworowski A,Crowe J,Loh L,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Enhancement of cytokine-driven NK cell IFN-γ production after vaccination of HCMV infected Africans.

European journal of immunology

Darboe A,Danso E,Clarke E,Umesi A,Touray E,Wegmuller R,Moore SE,Riley EM,Goodier MR

11-0279-42 was used in Flow Cytometry to investigate the impact of advanced NK cell differentiation on vaccine-induced responses.

Thu Jun 01 00:00:00 EDT 2017

Maintenance of the EBV-specific CD8⁺ TCRαβ repertoire in immunosuppressed lung transplant recipients.

Immunology and cell biology

Nguyen TH,Bird NL,Grant EJ,Miles JJ,Thomas PG,Kotsimbos TC,Mifsud NA,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Increased serum concentrations of IL-1 beta, IL-21 and Th17 cells in overweight patients with rheumatoid arthritis.

Arthritis research & therapy

Shoda H,Nagafuchi Y,Tsuchida Y,Sakurai K,Sumitomo S,Fujio K,Yamamoto K

11-0279-42 was used in Flow Cytometry to show individuals with BMI > 25 and rheumatoid arthritis had heightened concentrations of IL-1 beta, IL-21 and Th17 cells.

Wed May 31 00:00:00 EDT 2017

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Tue Aug 01 00:00:00 EDT 2017

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Oncoimmunology

Van Caeneghem Y,De Munter S,Tieppo P,Goetgeluk G,Weening K,Verstichel G,Bonte S,Taghon T,Leclercq G,Kerre T,Debets R,Vermijlen D,Abken H,Vandekerckhove B

Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

A human immunodeficiency syndrome caused by mutations in CARMIL2.

Nature communications

Schober T,Magg T,Laschinger M,Rohlfs M,Linhares ND,Puchalka J,Weisser T,Fehlner K,Mautner J,Walz C,Hussein K,Jaeger G,Kammer B,Schmid I,Bahia M,Pena SD,Behrends U,Belohradsky BH,Klein C,Hauck F

11-0279 was used in Flow cytometry/Cell sorting to show that human CARMIL2-deficiency is associated with defective CD28-mediated TCR co-signalling and impaired cytoskeletal dynamics.

Mon Jan 23 00:00:00 EST 2017

Immunophenotyping of rheumatoid arthritis reveals a linkage between HLA-DRB1 genotype, CXCR4 expression on memory CD4(+) T cells, and disease activity.

Scientific reports

Nagafuchi Y,Shoda H,Sumitomo S,Nakachi S,Kato R,Tsuchida Y,Tsuchiya H,Sakurai K,Hanata N,Tateishi S,Kanda H,Ishigaki K,Okada Y,Suzuki A,Kochi Y,Fujio K,Yamamoto K

11-0279 was used in Flow cytometry/Cell sorting to perform 24-subset immunophenotyping of peripheral blood mononuclear cells in HLA-DRB1-genotyped rheumatoid arthritis patients and healthy donors.

Thu Jul 07 00:00:00 EDT 2016

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

European journal of immunology

Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A

Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Long-term immune reconstitution of naive and memory t cell pools after haploidentical hematopoietic stem cell transplantation.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Azevedo RI,Soares MV,Albuquerque AS,Tendeiro R,Soares RS,Martins M,Ligeiro D,Victorino RM,Lacerda JF,Sousa AE

11-0279-42 was used in Flow Cytometry to show that long-term immune reconstitution was successfully achieved after haploidentical hematopoietic stem cell transplantation, a process that appears to have largely relied on de novo T cell production.

Wed May 01 00:00:00 EDT 2013

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM

Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Vigorous response of human innate functioning IgM memory B cells upon infection by Neisseria gonorrhoeae.

Journal of immunology (Baltimore, Md. : 1950)

So NS,Ostrowski MA,Gray-Owen SD

11-0279 was used in Flow cytometry/Cell sorting to observe the specific expansion of IgD(+)CD27(+) B cells in response to gonococcal infection.

Sun Apr 15 00:00:00 EDT 2012

Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.

Biotechnology and bioengineering

Kuzin I,Sun H,Moshkani S,Feng C,Mantalaris A,Wu JH,Bottaro A

Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842

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Entrez Gene ID: (Human) 939

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Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process

Process(es)

inflammatory response immune response cell surface receptor signaling pathway brain development release of cytoplasmic sequestered NF-kappaB extrinsic apoptotic signaling pathway via death domain receptors immunoglobulin mediated immune response response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to ethanol positive regulation of B cell differentiation positive regulation of JNK cascade negative regulation of T cell apoptotic process extrinsic apoptotic signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD27 Monoclonal Antibody (O323), FITC, eBioscience™

CD27 Antibody (11-0279-42) in Flow

Product Details

11-0279-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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