Invitrogen

CD28 Monoclonal Antibody (CD28.2), PE, eBioscience™

FIGURE: 1 / 18

CD28 Antibody (12-0289-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD28 PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-0289-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CD28.2

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2016668

Product Specific Information

Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported: The CD28.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The CD28.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CHI3L1 enhances melanoma lung metastasis via regulation of T cell co-stimulators and CTLA-4/B7 axis.

Frontiers in immunology

Ma B,Kamle S,Akosman B,Khan H,Lee CM,Lee CG,Elias JA

Published figure using CD28 monoclonal antibody (Product # 12-0289-42) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2023

Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.

Immunity

Szabo PA,Dogra P,Gray JI,Wells SB,Connors TJ,Weisberg SP,Krupska I,Matsumoto R,Poon MML,Idzikowski E,Morris SE,Pasin C,Yates AJ,Ku A,Chait M,Davis-Porada J,Guo XV,Zhou J,Steinle M,Mackay S,Saqi A,Baldwin MR,Sims PA,Farber DL

Published figure using CD28 monoclonal antibody (Product # 12-0289-42) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Effects of the Antidiabetic Drugs Evogliptin and Sitagliptin on the Immune Function of CD26/DPP4 in Th1 Cells.

Biomolecules & therapeutics

Yoon H,Sung JH,Song MJ

Published figure using CD28 monoclonal antibody (Product # 12-0289-42) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

CD19 Chimeric Antigen Receptor-Exosome Targets CD19 Positive B-lineage Acute Lymphocytic Leukemia and Induces Cytotoxicity.

Cancers

Haque S,Vaiselbuh SR

12-0289-42 was used in Flow Cytometry to show that the novel CD19 CAR exosomes target the CD19 surface antigens of leukemic B-cells and can induce contact-dependent cytotoxicity.

Fri Mar 19 00:00:00 EDT 2021

CD19 Chimeric Antigen Receptor-Exosome Targets CD19 Positive B-lineage Acute Lymphocytic Leukemia and Induces Cytotoxicity.

Cancers

Haque S,Vaiselbuh SR

12-0289-42 was used in Flow Cytometry to show that the novel CD19 CAR exosomes target the CD19 surface antigens of leukemic B-cells and can induce contact-dependent cytotoxicity.

Fri Mar 19 00:00:00 EDT 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

Published figure using CD28 monoclonal antibody (Product # 12-0289-42) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

SIRT1 is downregulated by autophagy in senescence and ageing.

Nature cell biology

Xu C,Wang L,Fozouni P,Evjen G,Chandra V,Jiang J,Lu C,Nicastri M,Bretz C,Winkler JD,Amaravadi R,Garcia BA,Adams PD,Ott M,Tong W,Johansen T,Dou Z,Berger SL

12-0289 was used in Flow cytometry/Cell sorting to investigate SIRT1 homeostasis in mammalian senescence and aging.

Thu Oct 01 00:00:00 EDT 2020

SIRT1 is downregulated by autophagy in senescence and ageing.

Nature cell biology

Xu C,Wang L,Fozouni P,Evjen G,Chandra V,Jiang J,Lu C,Nicastri M,Bretz C,Winkler JD,Amaravadi R,Garcia BA,Adams PD,Ott M,Tong W,Johansen T,Dou Z,Berger SL

12-0289 was used in Flow cytometry/Cell sorting to investigate SIRT1 homeostasis in mammalian senescence and aging.

Thu Oct 01 00:00:00 EDT 2020

PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2.

The Journal of cell biology

Xu X,Hou B,Fulzele A,Masubuchi T,Zhao Y,Wu Z,Hu Y,Jiang Y,Ma Y,Wang H,Bennett EJ,Fu G,Hui E

Published figure using CD28 monoclonal antibody (Product # 12-0289-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD28 monoclonal antibody (Product # 12-0289-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis.

Cancers

Darvekar S,Juzenas P,Oksvold M,Kleinauskas A,Holien T,Christensen E,Stokke T,Sioud M,Peng Q

Published figure using CD28 monoclonal antibody (Product # 12-0289-42) in Flow Cytometry

Thu Feb 06 00:00:00 EST 2020

Metabolic reprogramming of human CD8⁺ memory T cells through loss of SIRT1.

The Journal of experimental medicine

Jeng MY,Hull PA,Fei M,Kwon HS,Tsou CL,Kasler H,Ng CP,Gordon DE,Johnson J,Krogan N,Verdin E,Ott M

Published figure using CD28 monoclonal antibody (Product # 12-0289-42) in Flow Cytometry

Tue Jan 02 00:00:00 EST 2018

Co-expression of CD39 and CD103 identifies tumor-reactive CD8 T cells in human solid tumors.

Nature communications

Duhen T,Duhen R,Montler R,Moses J,Moudgil T,de Miranda NF,Goodall CP,Blair TC,Fox BA,McDermott JE,Chang SC,Grunkemeier G,Leidner R,Bell RB,Weinberg AD

12-0289 was used in Flow cytometry/Cell sorting to develop an approach for detecting tumour-reactive tumour-infiltrating CD8 T cells that will help define mechanisms of existing immunotherapy treatments.

Fri Jul 13 00:00:00 EDT 2018

Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis.

Frontiers in immunology

Lopes DM,Oliveira SC,Page B,Carvalho LP,Carvalho EM,Cardoso LS

12-0289 was used in Flow cytometry/Cell sorting to evaluate the potential of the S.mansoni Sm29 antigen to change aspects of profiles of monocyte derived dendritic cells and lymphocytes from subjects with cutaneous leishmaniasis.

Tue Oct 15 00:00:00 EDT 2019

CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer.

Oncoimmunology

Su S,Zou Z,Chen F,Ding N,Du J,Shao J,Li L,Fu Y,Hu B,Yang Y,Sha H,Meng F,Wei J,Huang X,Liu B

12-0289 was used in Flow cytometry/Cell sorting to study PD-1/PD-L1-mediated immune tolerance of Epstein-Barr virus-associated gastric cancer and provide a new strategy to break the tolerance for the T cell-based adoptive therapy.

Tue Jan 12 00:00:00 EST 2021

The Distribution of Human Stem Cell-like Memory T Cell in Lung Cancer.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Hong H,Gu Y,Sheng SY,Lu CG,Zou JY,Wu CY

Published figure using CD28 monoclonal antibody (Product # 12-0289-42) in Flow Cytometry

Thu Jan 04 00:00:00 EST 2018

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

12-0289 was used in Flow cytometry/Cell sorting to investigate the expression characteristics and clinical significance of CD28, PD-1, CTLA4 and BTLA on T-cells in patients with chronic HBV and the correlations among these receptors.

Mon Aug 01 00:00:00 EDT 2016

CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients.

Scientific reports

Su S,Hu B,Shao J,Shen B,Du J,Du Y,Zhou J,Yu L,Zhang L,Chen F,Sha H,Cheng L,Meng F,Zou Z,Huang X,Liu B

12-0289 was used in Flow cytometry/Cell sorting to explore the effects of genetic disruption of the gene PD-1 on T cell viability, IFN-γ production and cytotoxicity.

Thu Jan 28 00:00:00 EST 2016

Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.

Journal of immunology (Baltimore, Md. : 1950)

Wertheimer AM,Bennett MS,Park B,Uhrlaub JL,Martinez C,Pulko V,Currier NL,Nikolich-Žugich D,Kaye J,Nikolich-Žugich J

Published figure using CD28 monoclonal antibody (Product # 12-0289-42) in Flow Cytometry

Sat Mar 01 00:00:00 EST 2014

Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities.

Journal for immunotherapy of cancer

Kovacsovics-Bankowski M,Chisholm L,Vercellini J,Tucker CG,Montler R,Haley D,Newell P,Ma J,Tseng P,Wolf R,Vetto JT,Hammill C,Hansen P,Weinberg AD

Published figure using CD28 monoclonal antibody (Product # 12-0289-42) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2014

Intensity of the humoral response to cytomegalovirus is associated with the phenotypic and functional status of the immune system.

Journal of virology

Alonso Arias R,Moro-García MA,Echeverría A,Solano-Jaurrieta JJ,Suárez-García FM,López-Larrea C

12-0289 was used in Flow cytometry/Cell sorting to study the association of the phenotypic and functional status of the immune system with the intensity of the humoral response to Cytomegalovirus.

Mon Apr 01 00:00:00 EDT 2013

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

12-0289 was used in Flow cytometry/Cell sorting to demonstrate a method to measure autophagy levels in certain diseases, whilst determining the contribution of autophagy to the efficacy of drugs.

Sun Apr 01 00:00:00 EDT 2012

Mycolactone suppresses T cell responsiveness by altering both early signaling and posttranslational events.

Journal of immunology (Baltimore, Md. : 1950)

Boulkroun S,Guenin-Macé L,Thoulouze MI,Monot M,Merckx A,Langsley G,Bismuth G,Di Bartolo V,Demangel C

12-0289 was used in Flow cytometry/Cell sorting to investigate a novel type of T cell immunosuppressive agent, the potent activity of which may explain the defective cellular responses in Buruli ulcer patients.

Mon Feb 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CD28; CD28 antigen; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44; TP44

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Gene Aliases: CD28; Tp44

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UniProt ID: (Human) P10747

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Entrez Gene ID: (Human) 940

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Function(s)

protease binding SH3/SH2 adaptor activity protein binding coreceptor activity protein kinase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD28 Monoclonal Antibody (CD28.2), PE, eBioscience™

CD28 Antibody (12-0289-42) in Flow

Product Details

12-0289-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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