Invitrogen

CD28 Monoclonal Antibody (CD28.2), Alexa Fluor™ 700, eBioscience™

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CD28 Antibody (56-0289-42) in Flow

Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, eFluor 450 (Product # 48-0199-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 700 (Product # 56-4714-80) (left) or CD28 Monoclonal Antibody, Alexa Fluor 700 (right). Cells in the lymphocyte gate were used for analysis.

CD28 Antibody in Functional Assay (Functional)

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Product Details

56-0289-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 11 publications 11 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CD28.2

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815230

Product Specific Information

Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported: This CD28.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CD28.2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser

Target Information

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CHI3L1 enhances melanoma lung metastasis via regulation of T cell co-stimulators and CTLA-4/B7 axis.

Frontiers in immunology

Ma B,Kamle S,Akosman B,Khan H,Lee CM,Lee CG,Elias JA

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2023

Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.

Immunity

Szabo PA,Dogra P,Gray JI,Wells SB,Connors TJ,Weisberg SP,Krupska I,Matsumoto R,Poon MML,Idzikowski E,Morris SE,Pasin C,Yates AJ,Ku A,Chait M,Davis-Porada J,Guo XV,Zhou J,Steinle M,Mackay S,Saqi A,Baldwin MR,Sims PA,Farber DL

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Effects of the Antidiabetic Drugs Evogliptin and Sitagliptin on the Immune Function of CD26/DPP4 in Th1 Cells.

Biomolecules & therapeutics

Yoon H,Sung JH,Song MJ

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

CD19 Chimeric Antigen Receptor-Exosome Targets CD19 Positive B-lineage Acute Lymphocytic Leukemia and Induces Cytotoxicity.

Cancers

Haque S,Vaiselbuh SR

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

SIRT1 is downregulated by autophagy in senescence and ageing.

Nature cell biology

Xu C,Wang L,Fozouni P,Evjen G,Chandra V,Jiang J,Lu C,Nicastri M,Bretz C,Winkler JD,Amaravadi R,Garcia BA,Adams PD,Ott M,Tong W,Johansen T,Dou Z,Berger SL

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2.

The Journal of cell biology

Xu X,Hou B,Fulzele A,Masubuchi T,Zhao Y,Wu Z,Hu Y,Jiang Y,Ma Y,Wang H,Bennett EJ,Fu G,Hui E

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis.

Cancers

Darvekar S,Juzenas P,Oksvold M,Kleinauskas A,Holien T,Christensen E,Stokke T,Sioud M,Peng Q

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Flow Cytometry

Thu Feb 06 00:00:00 EST 2020

RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures.

ImmunoHorizons

Li D,Bradley T,Cain DW,Pedroza-Pacheco I,Aggelakopoulou M,Parks R,Barr M,Xia SM,Scearce R,Bowman C,Stevens G,Newman A,Hora B,Chen Y,Riebe K,Wang Y,Sempowski G,Saunders KO,Borrow P,Haynes BF

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Functional assay

Tue Nov 10 00:00:00 EST 2020

Metabolic reprogramming of human CD8⁺ memory T cells through loss of SIRT1.

The Journal of experimental medicine

Jeng MY,Hull PA,Fei M,Kwon HS,Tsou CL,Kasler H,Ng CP,Gordon DE,Johnson J,Krogan N,Verdin E,Ott M

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Flow Cytometry

Tue Jan 02 00:00:00 EST 2018

The Distribution of Human Stem Cell-like Memory T Cell in Lung Cancer.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Hong H,Gu Y,Sheng SY,Lu CG,Zou JY,Wu CY

Published figure using CD28 monoclonal antibody (Product # 56-0289-42) in Flow Cytometry

Thu Jan 04 00:00:00 EST 2018

Bioinformatics

Protein Aliases: CD28; CD28 antigen; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44; TP44

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Gene Aliases: CD28; Tp44

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UniProt ID: (Human) P10747

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Entrez Gene ID: (Human) 940

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Function(s)

protease binding SH3/SH2 adaptor activity protein binding coreceptor activity protein kinase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD28 Monoclonal Antibody (CD28.2), Alexa Fluor™ 700, eBioscience™

CD28 Antibody (56-0289-42) in Flow

Product Details

56-0289-42

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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