Invitrogen

CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), PE-Cyanine5, eBioscience™

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Antibody Testing Data (1)
Published Figures (3)

CD49b (Integrin alpha 2) Antibody in Flow Cytometry (Flow)

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CD49b (Integrin alpha 2) Antibody (15-5971-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 eFluor® 450 (Product # 48-5941-82) and 0.06 µg of Anti-Mouse CD49b (Integrin alpha 2) PE-Cyanine5.Cells in the lymphocyte gate were used for analysis.

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Product Details

15-5971-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

DX5

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573070

Product Specific Information

Description: The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer cells and a subset of T cells. DX5 reacts with all strains of mouse tested including the most commonly used strains, BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL and 129. Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.

Applications Reported: This DX5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Impact of mouse model tumor implantation site on acquired resistance to anti-PD-1 immune checkpoint therapy.

Frontiers in immunology

Denis M,Mathé D,Micoud M,Choffour PA,Grasselly C,Matera EL,Dumontet C

15-5971-82 was used in flow cytometry to demonstrate several differences between SC and orthotopic models at basal state, which tend to become similar after therapy.

Tue Jan 31 00:00:00 EST 2023

Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.

The Journal of experimental medicine

Hosokawa H,Koizumi M,Masuhara K,Romero-Wolf M,Tanaka T,Nakayama T,Rothenberg EV

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 15-5971-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Microbiota triggers STING-type I IFN-dependent monocyte reprogramming of the tumor microenvironment.

Cell

Lam KC,Araya RE,Huang A,Chen Q,Di Modica M,Rodrigues RR,Lopès A,Johnson SB,Schwarz B,Bohrnsen E,Cogdill AP,Bosio CM,Wargo JA,Lee MP,Goldszmid RS

15-5971-82 was used in Flow cytometry/Cell sorting to uncover a mechanistic link between the microbiota and the innate TME that can be harnessed to improve cancer therapies.

Thu Oct 14 00:00:00 EDT 2021

Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules.

NPJ vaccines

Andersen TK,Huszthy PC,Gopalakrishnan RP,Jacobsen JT,Fauskanger M,Tveita AA,Grødeland G,Bogen B

15-5971 was used in Flow cytometry/Cell sorting to study the underlying mechanism by which the targeting of antigen to MHCII on antigen-presenting cells increases antibody responses.

Thu Oct 05 00:00:00 EDT 2023

Differentiation and Protective Capacity of Virus-Specific CD8⁺ T Cells Suggest Murine Norovirus Persistence in an Immune-Privileged Enteric Niche.

Immunity

Tomov VT,Palko O,Lau CW,Pattekar A,Sun Y,Tacheva R,Bengsch B,Manne S,Cosma GL,Eisenlohr LC,Nice TJ,Virgin HW,Wherry EJ

15-5971 was used in Flow cytometry/Cell sorting to elucidate a strategy of immune evasion by mouse norovirus via the induction of a CD8+ T cell program normally reserved for latent pathogens and persistence in an immune-privileged enteric niche.

Tue Oct 17 00:00:00 EDT 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 15-5971-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

VprBP Is Required for Efficient Editing and Selection of Igκ+ B Cells, but Is Dispensable for Igλ+ and Marginal Zone B Cell Maturation and Selection.

Journal of immunology (Baltimore, Md. : 1950)

Palmer VL,Aziz-Seible R,Kassmeier MD,Rothermund M,Perry GA,Swanson PC

15-5971 was used in Flow cytometry/Cell sorting to determine the contribution of the protein, VprBP, to B cell development and selection.

Sat Aug 15 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; Integrin alpha-2; Platelet membrane glycoprotein Ia; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor

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Gene Aliases: CD49B; DX5; Itga2

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UniProt ID: (Mouse) Q62469

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Entrez Gene ID: (Mouse) 16398

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Function(s)

integrin binding collagen binding protein complex binding collagen receptor activity laminin binding metal ion binding protein heterodimerization activity collagen binding involved in cell-matrix adhesion integrin

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), PE-Cyanine5, eBioscience™

CD49b (Integrin alpha 2) Antibody (15-5971-82) in Flow

Product Details

15-5971-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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