Invitrogen

CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), eFluor™ 450, eBioscience™

7 Published Figures

23 References

View all (70) CD49b (Integrin alpha 2) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

CD49b (Integrin alpha 2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

CD49b (Integrin alpha 2) Antibody (48-5971-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and 0.5 µg of Anti-Mouse CD49b (Integrin alpha 2) eFluor® 450. Total viable cells were used for analysis.

CD49b (Integrin alpha 2) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

48-5971-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgM, kappa

Recommended Isotype Control

Rat IgM Isotype Control (eBRM), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DX5

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10671541

Product Specific Information

Description: The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer cells and a subset of T cells. DX5 reacts with all strains of mouse tested including the most commonly used strains, BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL and 129. Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.

Applications Reported: This DX5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 48-5971-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.

The Journal of experimental medicine

Hosokawa H,Koizumi M,Masuhara K,Romero-Wolf M,Tanaka T,Nakayama T,Rothenberg EV

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 48-5971-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

48-5971-82 was used in Flow Cytometry to demonstrate that ID2 sets a threshold for TCF1 during NK cell development, thus controlling the balance of immature and terminally differentiated cells that support future NK cell responses.

Mon Jun 07 00:00:00 EDT 2021

Exosomes derived from retinoblastoma cells enhance tumour deterioration by infiltrating the microenvironment.

Oncology reports

Chen S,Chen X,Qiu J,Chen P,Han X,Wu Y,Zhuang J,Yang M,Wu C,Wu N,Yang Y,Ge J,Yu K,Zhuang J

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 48-5971-82) in Immunohistochemistry

Fri Jan 01 00:00:00 EST 2021

Transplantation tolerance modifies donor-specific B cell fate to suppress de novo alloreactive B cells.

The Journal of clinical investigation

Khiew SH,Jain D,Chen J,Yang J,Yin D,Young JS,Dent A,Sciammas R,Alegre ML,Chong AS

48-5971 was used in Flow cytometry/Cell sorting to showed that alloreactive B cells were not deleted but rapidly lost their ability to differentiate into germinal center B cells and secrete donor-specific antibodies., by using a murine model of heart tolerance.

Wed Jul 01 00:00:00 EDT 2020

Inflammation-Induced Lactate Leads to Rapid Loss of Hepatic Tissue-Resident NK Cells.

Cell reports

Dodard G,Tata A,Erick TK,Jaime D,Miah SMS,Quatrini L,Escalière B,Ugolini S,Vivier E,Brossay L

48-5971 was used in Flow cytometry/Cell sorting to characterize the kinetics and immune response of each of the hepatic NK cell populations to viral challenge and other stimuli.

Tue Jul 07 00:00:00 EDT 2020

Natural killer cell monitoring in cutaneous melanoma - new dynamic biomarker.

Oncology letters

Isvoranu G,Surcel M,Huică RI,Munteanu AN,Pîrvu IR,Ciotaru D,Constantin C,Bratu O,Neagu M,Ursaciuc C

48-5971-82 was used in Flow Cytometry to show natural killer cells, in the spleen of mice carrying a melanoma, are reduced and that their phenotype changed.

Wed May 01 00:00:00 EDT 2019

MDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment.

Journal for immunotherapy of cancer

Fang DD,Tang Q,Kong Y,Wang Q,Gu J,Fang X,Zou P,Rong T,Wang J,Yang D,Zhai Y

48-5971-82 was used in Flow cytometry/Cell sorting to demonstrate that p53 activation mediated by APG-115 promotes antitumor immunity in the tumor microenvironment (TME) regardless of the Trp53 status of tumors per se.

Thu Nov 28 00:00:00 EST 2019

Intranasal delivery of adjuvant-free peptide nanofibers elicits resident CD8⁺ T cell responses.

Journal of controlled release : official journal of the Controlled Release Society

Si Y,Wen Y,Kelly SH,Chong AS,Collier JH

48-5971 was used in Flow cytometry/Cell sorting to investigate the extent to which self-assembled peptide nanofibers bearing an influenza peptide epitope elicit antigen-specific CD8+ T cell responses when delivered intranasally, and compare these responses with those elicited by subcutaneous immunization.

Sat Jul 28 00:00:00 EDT 2018

A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment.

eLife

Ng KK,Yui MA,Mehta A,Siu S,Irwin B,Pease S,Hirose S,Elowitz MB,Rothenberg EV,Kueh HY

48-5971 was used in Flow cytometry/Cell sorting to study the activation of Bcl11b, which controls T-cell fate commitment.

Tue Nov 20 00:00:00 EST 2018

Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease.

Cell

Van Dyken SJ,Liang HE,Naikawadi RP,Woodruff PG,Wolters PJ,Erle DJ,Locksley RM

48-5971 was used in Flow cytometry/Cell sorting to show that altered chitin clearance could exacerbate fibrogenic pathways in the setting of lung diseases characterised by epithelial cell dysfunction.

Thu Apr 20 00:00:00 EDT 2017

DC subset-specific induction of T cell responses upon antigen uptake via Fcγ receptors in vivo.

The Journal of experimental medicine

Lehmann CHK,Baranska A,Heidkamp GF,Heger L,Neubert K,Lühr JJ,Hoffmann A,Reimer KC,Brückner C,Beck S,Seeling M,Kießling M,Soulat D,Krug AB,Ravetch JV,Leusen JHW,Nimmerjahn F,Dudziak D

48-5971 was used in Flow cytometry/Cell sorting to suggest that targeting antigens to FcγRs on DCs may be a promising approach to raise immunogenic or tolerogenic T cell responses to antigens of choice.

Mon May 01 00:00:00 EDT 2017

4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells.

Journal of immunology (Baltimore, Md. : 1950)

Zilio S,Vella JL,De la Fuente AC,Daftarian PM,Weed DT,Kaifer A,Marigo I,Leone K,Bronte V,Serafini P

48-5971 was used in Flow cytometry/Cell sorting to study the use of 4PD nanoparticles for the in vivo gene silencing of tumour-educated myeloid cells.

Mon May 15 00:00:00 EDT 2017

Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.

PloS one

Ender F,Wiese AV,Schmudde I,Sun J,Vollbrandt T,König P,Laumonnier Y,Köhl J

48-5971 was used in Flow cytometry/Cell sorting to demonstrate a complex regulation pattern of C5aR1 in the airways, lung tissue and lymph nodes of mice.

Thu Aug 17 00:00:00 EDT 2017

CTLA4-Ig in combination with FTY720 promotes allograft survival in sensitized recipients.

JCI insight

Khiew SH,Yang J,Young JS,Chen J,Wang Q,Yin D,Vu V,Miller ML,Sciammas R,Alegre ML,Chong AS

48-5971 was used in Flow cytometry/Cell sorting to suggest inhibiting memory T cell expansion and trafficking into allografts with CTLA4-Ig and FTY720 promotes allograft survival in allosensitized recipients.

Thu May 04 00:00:00 EDT 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 48-5971-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

VprBP Is Required for Efficient Editing and Selection of Igκ+ B Cells, but Is Dispensable for Igλ+ and Marginal Zone B Cell Maturation and Selection.

Journal of immunology (Baltimore, Md. : 1950)

Palmer VL,Aziz-Seible R,Kassmeier MD,Rothermund M,Perry GA,Swanson PC

48-5971 was used in Flow cytometry/Cell sorting to determine the contribution of the protein, VprBP, to B cell development and selection.

Sat Aug 15 00:00:00 EDT 2015

Monitoring and cell-specific deletion of C5aR1 using a novel floxed GFP-C5aR1 reporter knock-in mouse.

Journal of immunology (Baltimore, Md. : 1950)

Karsten CM,Laumonnier Y,Eurich B,Ender F,Bröker K,Roy S,Czabanska A,Vollbrandt T,Figge J,Köhl J

48-5971 was used in Flow cytometry/Cell sorting to study cell-specific deletion of C5aR1 using a GFP-reporter knock-in mouse model.

Sun Feb 15 00:00:00 EST 2015

Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion.

European journal of immunology

Larsen J,Dall M,Antvorskov JC,Weile C,Engkilde K,Josefsen K,Buschard K

48-5971 was used in Flow cytometry/Cell sorting to study the in vitro and in vivo effects of gluten on natural killer cells.

Wed Oct 01 00:00:00 EDT 2014

A gluten-free diet lowers NKG2D and ligand expression in BALB/c and non-obese diabetic (NOD) mice.

Clinical and experimental immunology

Adlercreutz EH,Weile C,Larsen J,Engkilde K,Agardh D,Buschard K,Antvorskov JC

48-5971 was used in Flow cytometry/Cell sorting to show that a gluten-free diet reduces the level of NKG2D and the expression of NKG2D ligands.

Fri Aug 01 00:00:00 EDT 2014

Viral MHC class I-like molecule allows evasion of NK cell effector responses in vivo.

Journal of immunology (Baltimore, Md. : 1950)

Pyzik M,Dumaine A,Charbonneau B,Fodil-Cornu N,Jonjic S,Vidal SM

48-5971 was used in Flow cytometry/Cell sorting to show that recognition of the viral protein m157 by the inhibitory Ly49C receptor allows escape from natural killer cell control of viral replication.

Mon Dec 15 00:00:00 EST 2014

Helminth-induced interleukin-4 abrogates invariant natural killer T cell activation-associated clearance of bacterial infection.

Infection and immunity

Hsieh YJ,Fu CL,Hsieh MH

48-5971 was used in Flow cytometry/Cell sorting to reveal a previously unappreciated role for helminth-induced IL-4 in impairment of iNKT cell-mediated clearance of bacterial coexposure.

Thu May 01 00:00:00 EDT 2014

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 48-5971-82) in Immunofluorescence

Mon Dec 08 00:00:00 EST 2014

Bioinformatics

Protein Aliases: BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; Integrin alpha-2; Platelet membrane glycoprotein Ia; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor

View more View less

Gene Aliases: CD49B; DX5; Itga2

View more View less

UniProt ID: (Mouse) Q62469

View more View less

Entrez Gene ID: (Mouse) 16398

View more View less

Function(s)

integrin binding collagen binding protein complex binding collagen receptor activity laminin binding metal ion binding protein heterodimerization activity collagen binding involved in cell-matrix adhesion integrin

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), eFluor™ 450, eBioscience™

CD49b (Integrin alpha 2) Antibody (48-5971-82) in Flow

Product Details

48-5971-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!