Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), NovaFluor™ Yellow 730, eBioscience™

View all (100) CD61 (Integrin beta 3) antibodies
Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

M053T03Y07-A

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.1 µg/test
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

2C9.G3

Conjugate

NovaFluor™ Yellow 730 NovaFluor™ Yellow 730 NovaFluor™ Yellow 730

Excitation/Emission Max

551/730 nm View spectra spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_3099252 (AB_2937373)

Product Specific Information

Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .

Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.

NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 552 nm; Emission: 718 nm; Laser: 561 nm (Yellow) Laser

Our internal testing shows that NovaFluor Yellow 730 non-specifically stains B cells in SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference.

Target Information

CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin. The CD61 protein product is composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. CD61 is found along with the alpha IIb chain in platelets. Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling. Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD61; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; platelet gpIIIa; Platelet membrane glycoprotein IIIa

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Gene Aliases: CD61; GP3A; INGRB3; Itgb3

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UniProt ID: (Rat) Q8R2H2, (Mouse) O54890

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Entrez Gene ID: (Rat) 29302, (Mouse) 16416

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Function(s)
fibronectin binding protease binding protein disulfide isomerase activity integrin binding protein binding peptide binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding extracellular matrix binding fibrinogen binding receptor activity integrin
Process(es)
positive regulation of endothelial cell proliferation positive regulation of leukocyte migration protein folding cellular calcium ion homeostasis cell-substrate junction assembly cell adhesion cell-matrix adhesion integrin-mediated signaling pathway embryo implantation regulation of G-protein coupled receptor protein signaling pathway response to radiation positive regulation of endothelial cell migration negative regulation of macrophage derived foam cell differentiation positive regulation of fibroblast migration negative regulation of lipid storage response to activity smooth muscle cell migration positive regulation of smooth muscle cell migration cell growth cell migration platelet activation regulation of cell migration positive regulation of cell migration cell-substrate adhesion activation of protein kinase activity negative regulation of lipid transport positive regulation of cell adhesion mediated by integrin positive regulation of osteoblast proliferation heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading cellular response to drug response to platelet-derived growth factor apolipoprotein A-I-mediated signaling pathway positive regulation of MAPK cascade negative regulation of low-density lipoprotein particle receptor biosynthetic process positive regulation of angiogenesis positive regulation of bone resorption viral entry into host cell mesodermal cell differentiation positive regulation of smooth muscle cell proliferation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipoprotein metabolic process negative chemotaxis regulation of release of sequestered calcium ion into cytosol negative regulation of cell death positive regulation of ERK1 and ERK2 cascade platelet aggregation cellular response to mechanical stimulus positive regulation of glomerular mesangial cell proliferation positive regulation of adenylate cyclase-inhibiting opioid receptor signaling pathway cellular response to insulin-like growth factor stimulus positive regulation of osteoclast differentiation cell projection morphogenesis angiogenesis involved in wound healing
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