Invitrogen

CD62L (L-Selectin) Monoclonal Antibody (MEL-14), eFluor™ 450, eBioscience™

CD62L (L-Selectin) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 65

CD62L (L-Selectin) Antibody (48-0621-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.25 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or Anti-Mouse CD62L (L-Selectin) eFluor® 450 (right). Total viable cells were used for analysis.

CD62L (L-Selectin) Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

48-0621-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 58 publications 58 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MEL-14

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1963590

Product Specific Information

Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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48-0621-82 was used in Flow cytometry/Cell sorting to investigate the role of Bach2 in natural killer (NK) cell development, revealing that Bach2 expression peaked in immature CD27+CD11b+ cells, and its deficiency led to increased proportions of terminally differentiated NK cells with enhanced granzyme and cytokine production, as well as improved NK cell-mediated control of tumor metastasis.

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eLife

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Sirt6 Regulates the Development of Medullary Thymic Epithelial Cells and Contributes to the Establishment of Central Immune Tolerance.

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CD8⁺ T cells in the central nervous system of mice with herpes simplex infection are highly activated and express high levels of CCR5 and CXCR3.

Journal of neurovirology

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cDC1 Dependent Accumulation of Memory T Cells Is Required for Chronic Autoimmune Inflammation in Murine Testis.

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Jing Y,Cao M,Zhang B,Long X,Wang X

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

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Evaluation of DNA-Launched Virus-Like Particle Vaccines in an Immune Competent Mouse Model of Chikungunya Virus Infection.

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Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration.

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Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Immunocytochemistry

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PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

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Synchronous effects of targeted mitochondrial complex I inhibitors on tumor and immune cells abrogate melanoma progression.

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AbuEid M,McAllister DM,McOlash L,Harwig MC,Cheng G,Drouillard D,Boyle KA,Hardy M,Zielonka J,Johnson BD,Hill RB,Kalyanaraman B,Dwinell MB

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Theranostic near-infrared-IIb emitting nanoprobes for promoting immunogenic radiotherapy and abscopal effects against cancer metastasis.

Nature communications

Li H,Wang M,Huang B,Zhu SW,Zhou JJ,Chen DR,Cui R,Zhang M,Sun ZJ

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Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

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Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

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Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

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Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry.

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48-0621-82 was used in Flow Cytometry to provide a protocol for analysis of T cell activation and cytokine production in the thymus, spleen, and blood.

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Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry.

STAR protocols

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48-0621-82 was used in Flow Cytometry to provide a protocol for analysis of T cell activation and cytokine production in the thymus, spleen, and blood.

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Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

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Nature communications

Komban RJ,Strömberg A,Biram A,Cervin J,Lebrero-Fernández C,Mabbott N,Yrlid U,Shulman Z,Bemark M,Lycke N

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MALT1 Proteolytic Activity Suppresses Autoimmunity in a T Cell Intrinsic Manner.

Frontiers in immunology

Demeyer A,Skordos I,Driege Y,Kreike M,Hochepied T,Baens M,Staal J,Beyaert R

48-0621 was used in Flow cytometry/Cell sorting to establish that suppression of autoimmune responses is due to the T-cell intrinsic role of MALT1 proteolytic activity.

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Liang Z,Zhang L,Su H,Luan R,Na N,Sun L,Zhao Y,Zhang X,Zhang Q,Li J,Zhang L,Zhao Y

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C5aR1 regulates T follicular helper differentiation and chronic graft-versus-host disease bronchiolitis obliterans.

JCI insight

Verghese DA,Chun N,Paz K,Fribourg M,Woodruff TM,Flynn R,Hu Y,Xiong H,Zhang W,Yi Z,Du J,Blazar BR,Heeger PS

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Thu Dec 20 00:00:00 EST 2018

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

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Tue Oct 30 00:00:00 EDT 2018

A method for manual and automated multiplex RNAscope in situ hybridization and immunocytochemistry on cytospin samples.

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Chan S,Filézac de L'Etang A,Rangell L,Caplazi P,Lowe JB,Romeo V

48-0621 was used in Flow cytometry/Cell sorting to develop a method for manual and automated multiplex RNAscope in situ hybridisation and immunocytochemistry on cytospin samples.

Mon Apr 22 00:00:00 EDT 2019

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Antibody Tumor Targeting Is Enhanced by CD27 Agonists through Myeloid Recruitment.

Cancer cell

Turaj AH,Hussain K,Cox KL,Rose-Zerilli MJJ,Testa J,Dahal LN,Chan HTC,James S,Field VL,Carter MJ,Kim HJ,West JJ,Thomas LJ,He LZ,Keler T,Johnson PWM,Al-Shamkhani A,Thirdborough SM,Beers SA,Cragg MS,Glennie MJ,Lim SH

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Mon Dec 11 00:00:00 EST 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Mon Jun 05 00:00:00 EDT 2017

Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice.

Neurobiology of disease

MacPherson KP,Sompol P,Kannarkat GT,Chang J,Sniffen L,Wildner ME,Norris CM,Tansey MG

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice.

Journal of immunology (Baltimore, Md. : 1950)

Sitrin J,Suto E,Wuster A,Eastham-Anderson J,Kim JM,Austin CD,Lee WP,Behrens TW

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Tue Aug 15 00:00:00 EDT 2017

Batf3-dependent CD8α⁺ Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.

EBioMedicine

Li Y,Liu X,Duan W,Tian H,Zhu G,He H,Yao S,Yi S,Song W,Tang H

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Itk is required for Th9 differentiation via TCR-mediated induction of IL-2 and IRF4.

Nature communications

Gomez-Rodriguez J,Meylan F,Handon R,Hayes ET,Anderson SM,Kirby MR,Siegel RM,Schwartzberg PL

48-0621 was used in Magnetic cell separation to investigate the role of IL-9 in allergic asthma and autoimmunity, showing that Itk is required for Th9 differentiation via TCR-mediated induction of IL-2 and IRF4.

Thu Mar 03 00:00:00 EST 2016

Unexpected Role for Adaptive αβTh17 Cells in Acute Respiratory Distress Syndrome.

Journal of immunology (Baltimore, Md. : 1950)

Li JT,Melton AC,Su G,Hamm DE,LaFemina M,Howard J,Fang X,Bhat S,Huynh KM,O'Kane CM,Ingram RJ,Muir RR,McAuley DF,Matthay MA,Sheppard D

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Leptin receptor signaling in T cells is required for Th17 differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Reis BS,Lee K,Fanok MH,Mascaraque C,Amoury M,Cohn LB,Rogoz A,Dallner OS,Moraes-Vieira PM,Domingos AI,Mucida D

48-0621 was used in Flow cytometry/Cell sorting to show that leptin receptor signalling in T cells is required for Th17 differentiation.

Mon Jun 01 00:00:00 EDT 2015

Targeted Mutation of Nuclear Bone Morphogenetic Protein 2 Impairs Secondary Immune Response in a Mouse Model.

BioMed research international

Olsen DS,Goar WA,Nichols BA,Bailey KT,Christensen SL,Merriam KR,Reynolds PR,Wilson E,Weber KS,Bridgewater LC

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Toxoplasma gondii superinfection and virulence during secondary infection correlate with the exact ROP5/ROP18 allelic combination.

mBio

Jensen KD,Camejo A,Melo MB,Cordeiro C,Julien L,Grotenbreg GM,Frickel EM,Ploegh HL,Young L,Saeij JP

48-0621 was used in Flow cytometry/Cell sorting to investigate the interaction between infected host and Toxoplasma gondii strains in endemic regions, showing that secondary infection correlates with the ROP5/ROP18 allele combinations.

Tue Feb 24 00:00:00 EST 2015

The tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals.

Nature immunology

Salmond RJ,Brownlie RJ,Morrison VL,Zamoyska R

48-0621 was used in Flow cytometry/Cell sorting to study how loss-of-function PTPN22 alleles can lead to the population expansion of effector and/or memory T cells and a predisposition to human autoimmunity.

Mon Sep 01 00:00:00 EDT 2014

CCDC88B is a novel regulator of maturation and effector functions of T cells during pathological inflammation.

The Journal of experimental medicine

Kennedy JM,Fodil N,Torre S,Bongfen SE,Olivier JF,Leung V,Langlais D,Meunier C,Berghout J,Langat P,Schwartzentruber J,Majewski J,Lathrop M,Vidal SM,Gros P

48-0621 was used in Flow cytometry/Cell sorting to suggest that CCDC88B is the morbid gene underlying the pleiotropic effect of the 11q13 locus on inflammation.

Mon Dec 15 00:00:00 EST 2014

Myelin-specific T helper 17 cells promote adult hippocampal neurogenesis through indirect mechanisms.

F1000Research

Niebling J,E Rünker A,Schallenberg S,Kretschmer K,Kempermann G

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Fri Mar 06 00:00:00 EST 2020

STAT4 is required for IL-23 responsiveness in Th17 memory cells and NKT cells.

JAK-STAT

Glosson-Byers NL,Sehra S,Kaplan MH

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2015

Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4⁺ T cell immunity.

Nature immunology

Reis BS,Rogoz A,Costa-Pinto FA,Taniuchi I,Mucida D

48-0621 was used in Flow cytometry/Cell sorting to demonstrate plasticity in the CD4(+) T cell lineage that allows chronic exposure to luminal antigens without pathological inflammation.

Fri Mar 01 00:00:00 EST 2013

A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models.

The Journal of clinical investigation

Dai X,James RG,Habib T,Singh S,Jackson S,Khim S,Moon RT,Liggitt D,Wolf-Yadlin A,Buckner JH,Rawlings DJ

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

Hepatocyte-specific S100a8 and S100a9 transgene expression in mice causes Cxcl1 induction and systemic neutrophil enrichment.

Cell communication and signaling : CCS

Wiechert L,Németh J,Pusterla T,Bauer C,De Ponti A,Manthey S,Marhenke S,Vogel A,Klingmüller U,Hess J,Angel P

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Sat Dec 15 00:00:00 EST 2012

Modeling Sjögren's syndrome with Id3 conditional knockout mice.

Immunology letters

Guo Z,Li H,Han M,Xu T,Wu X,Zhuang Y

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Wed Mar 30 00:00:00 EDT 2011

IL-7 promotes T cell viability, trafficking, and functionality and improves survival in sepsis.

Journal of immunology (Baltimore, Md. : 1950)

Unsinger J,McGlynn M,Kasten KR,Hoekzema AS,Watanabe E,Muenzer JT,McDonough JS,Tschoep J,Ferguson TA,McDunn JE,Morre M,Hildeman DA,Caldwell CC,Hotchkiss RS

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2010

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 48-0621-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD62 antigen-like family member L; CD62L; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 1; Ly-22; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen

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Gene Aliases: AI528707; CD62L; L-selectin; LECAM-1; Lnhr; Ly-22; Ly-m22; Ly22; Lyam-1; Lyam1; Sell

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UniProt ID: (Mouse) P18337

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Entrez Gene ID: (Mouse) 20343

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Function(s)

protease binding protein binding carbohydrate binding cell adhesion molecule binding glycolipid binding

Process(es)

cell adhesion response to ATP regulation of apoptotic process positive regulation of neutrophil chemotaxis

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CD62L (L-Selectin) Monoclonal Antibody (MEL-14), eFluor™ 450, eBioscience™

CD62L (L-Selectin) Antibody (48-0621-82) in Flow

Product Details

48-0621-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

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