Invitrogen

IL-2 Monoclonal Antibody (JES6-5H4), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

20 Published Figures

30 References

View all (144) IL-2 antibodies

Datasheet

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IL-2 Antibody (25-7021-82) in Flow

Surface staining of mouse splenocytes stimulated with PMA/Ionomycin in the presence of Monensin with Anti-Mouse CD4 FITC (Product # 11-0042-82), followed by intracellular staining with 1.0 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82) (left) or 1.0 µg of Anti-Mouse IL-2 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

IL-2 Antibody — Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IL-2 clone JES6-5H4 stains a large subset of CD4+ T cells and a minor subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IL-2 (clone JES6-5H4). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis. {TM}

Product Details

25-7021-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 29 publications 29 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES6-5H4

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1235004

Product Specific Information

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported: This JES6-5H4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This JES6-5H4 antibody has been tested by intracellular staining and flow cytrometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Meningeal lymphatics regulate radiotherapy efficacy through modulating anti-tumor immunity.

Cell research

Zhou C,Ma L,Xu H,Huo Y,Luo J

25-7021-82 was used in Flow Cytometry to suggest an essential role of the MLV-CLN network in RT-triggered anti-tumor immunity, and highlight the potential of VEGF-C mRNA for brain tumor therapy.

Wed Jun 01 00:00:00 EDT 2022

Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection.

International journal of molecular sciences

Yang G,Wang J,Wang S,Zhu Y

Published figure using IL-2 monoclonal antibody (Product # 25-7021-82) in Flow Cytometry

Sat Aug 20 00:00:00 EDT 2022

In vivo genome-wide CRISPR screening identifies ZNF24 as a negative NF-κB modulator in lung cancer.

Cell & bioscience

Liu L,Lei Y,Chen W,Zhou Q,Zheng Z,Zeng G,Liu W,Feng P,Zhang Z,Yu L,Chen L

Published figure using IL-2 monoclonal antibody (Product # 25-7021-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Z. morio Hemolymph Relieves E. coli-Induced Mastitis by Inhibiting Inflammatory Response and Repairing the Blood-Milk Barrier.

International journal of molecular sciences

Zou Y,Wang X,Xu J,Wang S,Li S,Zhu Y,Wang J

Published figure using IL-2 monoclonal antibody (Product # 25-7021-82) in Flow Cytometry

Mon Oct 31 00:00:00 EDT 2022

Impaired Treg-DC interactions contribute to autoimmunity in leukocyte adhesion deficiency type 1.

JCI insight

Klaus T,Wilson AS,Vicari E,Hadaschik E,Klein M,Helbich SSC,Kamenjarin N,Hodapp K,Schunke J,Haist M,Butsch F,Probst HC,Enk AH,Mahnke K,Waisman A,Bednarczyk M,Bros M,Bopp T,Grabbe S

25-7021-82 was used in Flow cytometry/Cell sorting to demonstrate a crucial role of LFA-1 on Treg to maintain immune homeostasis by modifying T cell-DC interactions and CD4+ T cell activation.

Thu Dec 22 00:00:00 EST 2022

Impaired Treg-DC interactions contribute to autoimmunity in leukocyte adhesion deficiency type 1.

JCI insight

Klaus T,Wilson AS,Vicari E,Hadaschik E,Klein M,Helbich SSC,Kamenjarin N,Hodapp K,Schunke J,Haist M,Butsch F,Probst HC,Enk AH,Mahnke K,Waisman A,Bednarczyk M,Bros M,Bopp T,Grabbe S

25-7021-82 was used in Flow cytometry/Cell sorting to demonstrate a crucial role of LFA-1 on Treg to maintain immune homeostasis by modifying T cell-DC interactions and CD4+ T cell activation.

Thu Dec 22 00:00:00 EST 2022

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination.

EMBO molecular medicine

Müller K,Gibbins MP,Roberts M,Reyes-Sandoval A,Hill AVS,Draper SJ,Matuschewski K,Silvie O,Hafalla JCR

25-7021-82 was used in Flow Cytometry to test the impact of pre-erythrocytic malaria immunogenicity on the immune system response.

Fri Apr 09 00:00:00 EDT 2021

A Methyltransferase-Defective Vesicular Stomatitis Virus-Based SARS-CoV-2 Vaccine Candidate Provides Complete Protection against SARS-CoV-2 Infection in Hamsters.

Journal of virology

Lu M,Zhang Y,Dravid P,Li A,Zeng C,Kc M,Trivedi S,Sharma H,Chaiwatpongsakorn S,Zani A,Kenney A,Cai C,Ye C,Liang X,Qiu J,Martinez-Sobrido L,Yount JS,Boyaka PN,Liu SL,Peeples ME,Kapoor A,Li J

Published figure using IL-2 monoclonal antibody (Product # 25-7021-82) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using IL-2 monoclonal antibody (Product # 25-7021-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 pneumonia in rhesus macaques.

Nature

van Doremalen N,Lambe T,Spencer A,Belij-Rammerstorfer S,Purushotham JN,Port JR,Avanzato VA,Bushmaker T,Flaxman A,Ulaszewska M,Feldmann F,Allen ER,Sharpe H,Schulz J,Holbrook M,Okumura A,Meade-White K,Pérez-Pérez L,Edwards NJ,Wright D,Bissett C,Gilbride C,Williamson BN,Rosenke R,Long D,Ishwarbhai A,Kailath R,Rose L,Morris S,Powers C,Lovaglio J,Hanley PW,Scott D,Saturday G,de Wit E,Gilbert SC,Munster VJ

25-7021 was used in Flow cytometry/Cell sorting to demonstrate the efficacy of the adenovirus-vector-based vaccine ChAdOx1 nCoV-19 in rhesus macaques.

Thu Oct 01 00:00:00 EDT 2020

Heterogenous Populations of Tissue-Resident CD8⁺ T Cells Are Generated in Response to Infection and Malignancy.

Immunity

Milner JJ,Toma C,He Z,Kurd NS,Nguyen QP,McDonald B,Quezada L,Widjaja CE,Witherden DA,Crowl JT,Shaw LA,Yeo GW,Chang JT,Omilusik KD,Goldrath AW

25-7021 was used in Flow cytometry/Cell sorting to identify discrete lineages of intestinal antigen-specific CD8+ T cells, including a Blimp1hiId3lo tissue-resident effector cell population most prominent in the early phase of acute viral and bacterial infections and a molecularly distinct Blimp1loId3hi tissue-resident memory population that subsequently accumulated at later infection time points.

Tue May 19 00:00:00 EDT 2020

Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors.

EMBO molecular medicine

Fan Z,Tian Y,Chen Z,Liu L,Zhou Q,He J,Coleman J,Dong C,Li N,Huang J,Xu C,Zhang Z,Gao S,Zhou P,Ding K,Chen L

Published figure using IL-2 monoclonal antibody (Product # 25-7021-82) in Flow Cytometry

Mon Jun 08 00:00:00 EDT 2020

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

Vaccines

Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

25-7021 was used in Flow cytometry/Cell sorting to study the protection mediated by liver-stage (LS) antigens as vaccine components for malaria parasite infection.

Fri Jan 10 00:00:00 EST 2020

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

Vaccines

Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

25-7021 was used in Flow cytometry/Cell sorting to study the protection mediated by liver-stage (LS) antigens as vaccine components for malaria parasite infection.

Fri Jan 10 00:00:00 EST 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

25-7021 was used in Flow cytometry/Cell sorting to provide a framework for effective vaccination against cancer-associated herpesviruses through the elimination of latency and key immune evasion mechanisms from the pathogen.

Thu Nov 05 00:00:00 EST 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Thu Nov 05 00:00:00 EST 2020

Blockade of CTLA-4 and PD-1 Enhances Adoptive T-cell Therapy Efficacy in an ICOS-Mediated Manner.

Cancer immunology research

Shi LZ,Goswami S,Fu T,Guan B,Chen J,Xiong L,Zhang J,Ng Tang D,Zhang X,Vence L,Blando J,Allison JP,Collazo R,Gao J,Sharma P

25-7021 was used in Flow cytometry/Cell sorting to provides a foundation of testing combinatorial therapy of ACT of CD8 T cells and dual blocking of CTLA-4 and PD-1 in patients with melanoma.

Fri Nov 01 00:00:00 EDT 2019

Protective immunity by an engineered DNA vaccine for Mayaro virus.

PLoS neglected tropical diseases

Choi H,Kudchodkar SB,Reuschel EL,Asija K,Borole P,Ho M,Wojtak K,Reed C,Ramos S,Bopp NE,Aguilar PV,Weaver SC,Kim JJ,Humeau L,Tebas P,Weiner DB,Muthumani K

25-7021 was used in Flow cytometry/Cell sorting to develop and test a novel DNA vaccine for Mayaro virus which encodes a synthetically designed consesus Mayaro envelope sequence.

Fri Feb 01 00:00:00 EST 2019

Stabilization of cytokine mRNAs in iNKT cells requires the serine-threonine kinase IRE1alpha.

Nature communications

Govindarajan S,Gaublomme D,Van der Cruyssen R,Verheugen E,Van Gassen S,Saeys Y,Tavernier S,Iwawaki T,Bloch Y,Savvides SN,Lambrecht BN,Janssens S,Elewaut D,Drennan MB

25-7021 was used in Flow cytometry/Cell sorting to examine the role of the IRE1α-dependent signalling cascade in coupling constitutive cytokine mRNA expression to the rapid induction of cytokine secretion and effector functions in activated iNKT cells.

Mon Dec 17 00:00:00 EST 2018

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

25-7021 was used in Flow cytometry/Cell sorting to show that miR-27 has a role in regulating Treg immune cells and that correct control of miR-27 expression is important to safeguard immunological tolerance..

Wed Feb 01 00:00:00 EST 2017

Vaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8⁺ T Cells.

Cancer immunology research

Zahm CD,Colluru VT,McNeel DG

Published figure using IL-2 monoclonal antibody (Product # 25-7021-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Published figure using IL-2 monoclonal antibody (Product # 25-7021-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using IL-2 monoclonal antibody (Product # 25-7021-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

EBioMedicine

Ma J,Teng X,Wang X,Fan X,Wu Y,Tian M,Zhou Z,Li L

Published figure using IL-2 monoclonal antibody (Product # 25-7021-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Loss of Gα_i proteins impairs thymocyte development, disrupts T-cell trafficking, and leads to an expanded population of splenic CD4⁺PD-1⁺CXCR5^+/- T-cells.

Scientific reports

Hwang IY,Harrison K,Park C,Kehrl JH

25-7021 was used in Flow cytometry/Cell sorting to delineate a role for Gαi2 in early thymocyte development and for Gαi2/3 in multiple aspects of T cell biology.

Fri Jun 23 00:00:00 EDT 2017

A synthetic consensus anti-spike protein DNA vaccine induces protective immunity against Middle East respiratory syndrome coronavirus in nonhuman primates.

Science translational medicine

Muthumani K,Falzarano D,Reuschel EL,Tingey C,Flingai S,Villarreal DO,Wise M,Patel A,Izmirly A,Aljuaid A,Seliga AM,Soule G,Morrow M,Kraynyak KA,Khan AS,Scott DP,Feldmann F,LaCasse R,Meade-White K,Okumura A,Ugen KE,Sardesai NY,Kim JJ,Kobinger G,Feldmann H,Weiner DB

25-7021 was used in Flow cytometry/Cell sorting to report on the development of a synthetic DNA vaccine against a novel member of the lineage C betacoronaviruses.

Wed Aug 19 00:00:00 EDT 2015

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using IL-2 monoclonal antibody (Product # 25-7021-82) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

DNA vaccines targeting heavy chain C-terminal fragments of Clostridium botulinum neurotoxin serotypes A, B, and E induce potent humoral and cellular immunity and provide protection from lethal toxin challenge.

Human vaccines & immunotherapeutics

Scott VL,Villarreal DO,Hutnick NA,Walters JN,Ragwan E,Bdeir K,Yan J,Sardesai NY,Finnefrock AC,Casimiro DR,Weiner DB

25-7021 was used in Flow cytometry/Cell sorting to demonstrate the protective efficacy of a combinative synthetic DNA vaccine approach targeting three important C. botulinum neurotoxin serotypes.

Wed May 04 00:00:00 EDT 2016

TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.

European journal of immunology

Reba SM,Li Q,Onwuzulike S,Ding X,Karim AF,Hernandez Y,Fulton SA,Harding CV,Lancioni CL,Nagy N,Rodriguez ME,Wearsch PA,Rojas RE

25-7021-82 was used in Flow cytometry/Cell sorting to examine the role of CD4(+) T-cell-expressed TLR2 in Mycobacterium tuberculosis (MTB) Ag-specific T-cell priming and in protection against MTB infection in vivo.

Thu May 01 00:00:00 EDT 2014

TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.

European journal of immunology

Reba SM,Li Q,Onwuzulike S,Ding X,Karim AF,Hernandez Y,Fulton SA,Harding CV,Lancioni CL,Nagy N,Rodriguez ME,Wearsch PA,Rojas RE

Thu May 01 00:00:00 EDT 2014

CD40 ligand preferentially modulates immune response and enhances protection against influenza virus.

Journal of immunology (Baltimore, Md. : 1950)

Hashem AM,Gravel C,Chen Z,Yi Y,Tocchi M,Jaentschke B,Fan X,Li C,Rosu-Myles M,Pereboev A,He R,Wang J,Li X

25-7021 was used in Flow cytometry/Cell sorting to characterise an increased in vivo load of CD40-targeted Ag upon the secretion of the NP-CD40L fusion protein from adenovirus-infected cells.

Tue Jul 15 00:00:00 EDT 2014

Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine.

Vaccine

Yan J,Villarreal DO,Racine T,Chu JS,Walters JN,Morrow MP,Khan AS,Sardesai NY,Kim JJ,Kobinger GP,Weiner DB

25-7021 was used in Flow cytometry/Cell sorting to investigate how synthetic DNA vaccine is a new tool for targeting emerging infection and providing protective immunity to H7N9 influenza viruses.

Mon May 19 00:00:00 EDT 2014

Epstein-Barr virus latent membrane protein 2A exacerbates experimental autoimmune encephalomyelitis and enhances antigen presentation function.

Scientific reports

Chang RA,Miller SD,Longnecker R

25-7021 was used in an ELISpot assay to investigate the Epstein-Barr virus as a possible etiological agent involved in multiple sclerosis.

Mon Feb 25 00:00:00 EST 2013

Autoreactive Tbet-positive CD4 T cells develop independent of classic Th1 cytokine signaling during experimental autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Yeh WI,McWilliams IL,Harrington LE

25-7021 was used in Flow cytometry/Cell sorting to study changes in expression of Tbet by CD4 T cells from distinct areas of the body following the onset of experimental autoimmune encephalomyelitis.

Tue Nov 15 00:00:00 EST 2011

Bioinformatics

Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: Il-2; Il2

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UniProt ID: (Mouse) P04351

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Entrez Gene ID: (Mouse) 16183

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Function(s)

cytokine activity interleukin-2 receptor binding growth factor activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding actin or actin-binding cytoskeletal protein non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-2 Monoclonal Antibody (JES6-5H4), PE-Cyanine7, eBioscience™

IL-2 Antibody (25-7021-82) in Flow

Product Details

25-7021-82

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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