Invitrogen

IL-2 Monoclonal Antibody (JES6-5H4), eFluor™ 450, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

23 Published Figures

27 References

View all (144) IL-2 antibodies

Datasheet

Protocols

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Datasheet

Protocols

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FIGURE: 1 / 25

IL-2 Antibody (48-7021-82) in Flow

Intracellular staining of PMA/ionomycin stimulated mouse splenoytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.25 µg of Rat IgG2b K Isotype Control eFluor® 450 (Product # 48-4031-82) (left) or 0.25 µg of Anti-Mouse IL-2 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

IL-2 Antibody — Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IL-2 clone JES6-5H4 stains a large subset of CD4+ T cells and a minor subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IL-2 (clone JES6-5H4). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis. {TM}

Product Details

48-7021-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 23 publications 23 publications

ELISA (ELISA)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES6-5H4

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1944462

Product Specific Information

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported: This JES6-5H4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This JES6-5H4 antibody has been tested by intracellular staining and flow cytrometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection.

International journal of molecular sciences

Yang G,Wang J,Wang S,Zhu Y

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Sat Aug 20 00:00:00 EDT 2022

In vivo genome-wide CRISPR screening identifies ZNF24 as a negative NF-κB modulator in lung cancer.

Cell & bioscience

Liu L,Lei Y,Chen W,Zhou Q,Zheng Z,Zeng G,Liu W,Feng P,Zhang Z,Yu L,Chen L

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Z. morio Hemolymph Relieves E. coli-Induced Mastitis by Inhibiting Inflammatory Response and Repairing the Blood-Milk Barrier.

International journal of molecular sciences

Zou Y,Wang X,Xu J,Wang S,Li S,Zhu Y,Wang J

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Mon Oct 31 00:00:00 EDT 2022

Impaired Treg-DC interactions contribute to autoimmunity in leukocyte adhesion deficiency type 1.

JCI insight

Klaus T,Wilson AS,Vicari E,Hadaschik E,Klein M,Helbich SSC,Kamenjarin N,Hodapp K,Schunke J,Haist M,Butsch F,Probst HC,Enk AH,Mahnke K,Waisman A,Bednarczyk M,Bros M,Bopp T,Grabbe S

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Thu Dec 22 00:00:00 EST 2022

A Methyltransferase-Defective Vesicular Stomatitis Virus-Based SARS-CoV-2 Vaccine Candidate Provides Complete Protection against SARS-CoV-2 Infection in Hamsters.

Journal of virology

Lu M,Zhang Y,Dravid P,Li A,Zeng C,Kc M,Trivedi S,Sharma H,Chaiwatpongsakorn S,Zani A,Kenney A,Cai C,Ye C,Liang X,Qiu J,Martinez-Sobrido L,Yount JS,Boyaka PN,Liu SL,Peeples ME,Kapoor A,Li J

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

Long-Term Programming of CD8 T Cell Immunity by Perinatal Exposure to Glucocorticoids.

Cell

Hong JY,Lim J,Carvalho F,Cho JY,Vaidyanathan B,Yu S,Annicelli C,Ip WKE,Medzhitov R

48-7021 was used in Flow cytometry/Cell sorting to demonstrate that perinatal stress can have long-term consequences on CD8 T cell immunity by altering HPA axis activity.

Thu Mar 05 00:00:00 EST 2020

Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors.

EMBO molecular medicine

Fan Z,Tian Y,Chen Z,Liu L,Zhou Q,He J,Coleman J,Dong C,Li N,Huang J,Xu C,Zhang Z,Gao S,Zhou P,Ding K,Chen L

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Mon Jun 08 00:00:00 EDT 2020

Deletion Timing of Cic Alleles during Hematopoiesis Determines the Degree of Peripheral CD4⁺ T Cell Activation and Proliferation.

Immune network

Park GY,Lee GW,Kim S,Hong H,Park JS,Cho JH,Lee Y

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in ELISA

Thu Oct 01 00:00:00 EDT 2020

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

Vaccines

Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Thu Nov 05 00:00:00 EST 2020

Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses.

Cell reports

Blagih J,Zani F,Chakravarty P,Hennequart M,Pilley S,Hobor S,Hock AK,Walton JB,Morton JP,Gronroos E,Mason S,Yang M,McNeish I,Swanton C,Blyth K,Vousden KH

48-7021 was used in Flow cytometry/Cell sorting to show suppressive regulatory T cells accumulate in tumour cells in the absence of p53 indicating a pathway in which cancer cells circumvent immune destruction.

Tue Jan 14 00:00:00 EST 2020

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Vaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8⁺ T Cells.

Cancer immunology research

Zahm CD,Colluru VT,McNeel DG

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Differential Inhibitory Receptor Expression on T Cells Delineates Functional Capacities in Chronic Viral Infection.

Journal of virology

Teigler JE,Zelinskyy G,Eller MA,Bolton DL,Marovich M,Gordon AD,Alrubayyi A,Alter G,Robb ML,Martin JN,Deeks SG,Michael NL,Dittmer U,Streeck H

48-7021 was used in Flow cytometry/Cell sorting to determine and compare inhibitory receptor expression patterns on virus-specific CD4 and CD8 T cells in relation to their functional T cell profile.

Fri Dec 01 00:00:00 EST 2017

Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5.

Nature communications

Park S,Lee S,Lee CG,Park GY,Hong H,Lee JS,Kim YM,Lee SB,Hwang D,Choi YS,Fryer JD,Im SH,Lee SW,Lee Y

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in ELISA

Wed Jul 12 00:00:00 EDT 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

EBioMedicine

Ma J,Teng X,Wang X,Fan X,Wu Y,Tian M,Zhou Z,Li L

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth.

Nature communications

Klein JC,Moses K,Zelinskyy G,Sody S,Buer J,Lang S,Helfrich I,Dittmer U,Kirschning CJ,Brandau S

48-7021 was used in Flow cytometry/Cell sorting to demonstrate that cells deficient in endosomal TLR3, 7 and 9 leads to tumour regression and anti-tumour immunity.

Thu Mar 16 00:00:00 EDT 2017

The MAPK-Activated Kinase MK2 Attenuates Dendritic Cell-Mediated Th1 Differentiation and Autoimmune Encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Soukup K,Halfmann A,Le Bras M,Sahin E,Vittori S,Poyer F,Schuh C,Luger R,Niederreiter B,Haider T,Stoiber D,Blüml S,Schabbauer G,Kotlyarov A,Gaestel M,Felzmann T,Dohnal AM

48-7021 was used in Flow cytometry/Cell sorting to investigate the role of MK2 in dendritic cells, showing that it attenuates dendritic cell-mediated Th1 differentiation and autoimmune encephalomyelitis.

Wed Jul 15 00:00:00 EDT 2015

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.

European journal of immunology

Reba SM,Li Q,Onwuzulike S,Ding X,Karim AF,Hernandez Y,Fulton SA,Harding CV,Lancioni CL,Nagy N,Rodriguez ME,Wearsch PA,Rojas RE

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in Flow Cytometry

Thu May 01 00:00:00 EDT 2014

Macrophage PTEN regulates expression and secretion of arginase I modulating innate and adaptive immune responses.

Journal of immunology (Baltimore, Md. : 1950)

Sahin E,Haubenwallner S,Kuttke M,Kollmann I,Halfmann A,Dohnal AM,Chen L,Cheng P,Hoesel B,Einwallner E,Brunner J,Kral JB,Schrottmaier WC,Thell K,Saferding V,Blüml S,Schabbauer G

48-7021 was used in Flow cytometry/Cell sorting to study the role of extracellular Arginase I in inflammation and immunity.

Fri Aug 15 00:00:00 EDT 2014

Th2 cell-intrinsic hypo-responsiveness determines susceptibility to helminth infection.

PLoS pathogens

van der Werf N,Redpath SA,Azuma M,Yagita H,Taylor MD

48-7021 was used in Flow cytometry/Cell sorting to demonstrate that during chronic Litomosoides sigmodontis infection, CD4(+) Th2 cells are conditioned towards an intrinsically hypo-responsive phenotype.

Fri Mar 01 00:00:00 EST 2013

Modulation of pulmonary dendritic cell function during mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Liu Y,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in ELISA

Fri Feb 01 00:00:00 EST 2008

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 48-7021-82) in ELISA

Tue May 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: Il-2; Il2

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UniProt ID: (Mouse) P04351

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Entrez Gene ID: (Mouse) 16183

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Function(s)

cytokine activity interleukin-2 receptor binding growth factor activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding actin or actin-binding cytoskeletal protein non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-2 Monoclonal Antibody (JES6-5H4), eFluor™ 450, eBioscience™

IL-2 Antibody (48-7021-82) in Flow

Product Details

48-7021-82

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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