Invitrogen

IgM Monoclonal Antibody (II/41), PE-Cyanine5, eBioscience™

31 Published Figures

32 References

View all (30) Mouse IgM antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (31)

FIGURE: 1 / 32

IgM Antibody (15-5790-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2a Isotype Control PE-Cyanine5 (Product # 15-4321-80) (left) or 0.06 µg of Anti-Mouse IgM PE-Cyanine5 (right).Total viable cells were used for analysis.

Product Image Gallery

Product Details

15-5790-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 32 publications 32 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

II/41

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_494222

Product Specific Information

Description: The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Dlk1 maintains adult mice long-term HSCs by activating Notch signaling to restrict mitochondrial metabolism.

Experimental hematology & oncology

Huang D,Han Y,Tang T,Yang L,Jiang P,Qian W,Zhang Z,Qian X,Zeng X,Qian P

15-5790-82 was used in Flow cytometry/Cell sorting to demonstrate that Dlk1 is essential for maintaining HSC homeostasis, which is realized by governing Notch signalling and restricting mitochondrial metabolic activity.

Wed Jan 18 00:00:00 EST 2023

Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants.

Nature

Xu J,Xu K,Jung S,Conte A,Lieberman J,Muecksch F,Lorenzi JCC,Park S,Schmidt F,Wang Z,Huang Y,Luo Y,Nair MS,Wang P,Schulz JE,Tessarollo L,Bylund T,Chuang GY,Olia AS,Stephens T,Teng IT,Tsybovsky Y,Zhou T,Munster V,Ho DD,Hatziioannou T,Bieniasz PD,Nussenzweig MC,Kwong PD,Casellas R

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

A platform-agnostic, function first-based antibody discovery strategy using plasmid-free mammalian expression of antibodies.

mAbs

Zhang R,Prabakaran P,Yu X,Mackness BC,Boudanova E,Hopke J,Sancho J,Saleh J,Cho H,Zhang N,Simonds-Mannes H,Stimple SD,Hoffmann D,Park A,Chowdhury PS,Rao SP

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Wed Jan 19 00:00:00 EST 2022

Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers.

PloS one

Cohen AA,Yang Z,Gnanapragasam PNP,Ou S,Dam KA,Wang H,Bjorkman PJ

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Fri Oct 15 00:00:00 EDT 2021

B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches.

Cell reports

Biram A,Winter E,Denton AE,Zaretsky I,Dassa B,Bemark M,Linterman MA,Yaari G,Shulman Z

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Tue Feb 11 00:00:00 EST 2020

Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses.

Cell reports

Fallet B,Hao Y,Florova M,Cornille K,de Los Aires AV,Girelli Zubani G,Ertuna YI,Greiff V,Menzel U,Hammad K,Merkler D,Reddy ST,Weill JC,Reynaud CA,Pinschewer DD

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Tue Jan 28 00:00:00 EST 2020

TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells.

The FEBS journal

Schoeler K,Aufschnaiter A,Messner S,Derudder E,Herzog S,Villunger A,Rajewsky K,Labi V

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells.

Cell reports

Zhao M,Tao F,Venkatraman A,Li Z,Smith SE,Unruh J,Chen S,Ward C,Qian P,Perry JM,Marshall H,Wang J,He XC,Li L

15-5790 was used in Flow cytometry/Cell sorting to report that N-cad+ cells were functional bone and marrow stromal progenitor cells, giving rise to osteoblasts, adipocytes, and chondrocytes both in vitro and in vivo.

Tue Jan 15 00:00:00 EST 2019

A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Retinoid-Sensitive Epigenetic Regulation of the Hoxb Cluster Maintains Normal Hematopoiesis and Inhibits Leukemogenesis.

Cell stem cell

Qian P,De Kumar B,He XC,Nolte C,Gogol M,Ahn Y,Chen S,Li Z,Xu H,Perry JM,Hu D,Tao F,Zhao M,Han Y,Hall K,Peak A,Paulson A,Zhao C,Venkatraman A,Box A,Perera A,Haug JS,Parmely T,Li H,Krumlauf R,Li L

15-5790 was used in Flow cytometry/Cell sorting to demonstrate roles for retinoid signaling and the DERARE in maintaining HSCs and preventing leukemogenesis by coordinate regulation of Hoxb genes.

Thu May 03 00:00:00 EDT 2018

Vitamin A-Retinoic Acid Signaling Regulates Hematopoietic Stem Cell Dormancy.

Cell

Cabezas-Wallscheid N,Buettner F,Sommerkamp P,Klimmeck D,Ladel L,Thalheimer FB,Pastor-Flores D,Roma LP,Renders S,Zeisberger P,Przybylla A,Schönberger K,Scognamiglio R,Altamura S,Florian CM,Fawaz M,Vonficht D,Tesio M,Collier P,Pavlinic D,Geiger H,Schroeder T,Benes V,Dick TP,Rieger MA,Stegle O,Trumpp A

15-5790-82 was used in Flow Cytometry to highlight the impact of dietary vitamin A on the regulation of cell-cycle-mediated stem cell plasticity.

Thu May 18 00:00:00 EDT 2017

Conversion of T cells to B cells by inactivation of polycomb-mediated epigenetic suppression of the B-lineage program.

Genes & development

Ikawa T,Masuda K,Endo TA,Endo M,Isono K,Koseki Y,Nakagawa R,Kometani K,Takano J,Agata Y,Katsura Y,Kurosaki T,Vidal M,Koseki H,Kawamoto H

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2016

Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.

Proceedings of the National Academy of Sciences of the United States of America

Lu G,Duan J,Shu S,Wang X,Gao L,Guo J,Zhang Y

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Tue Feb 02 00:00:00 EST 2016

Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system.

Nature communications

Cheong TC,Compagno M,Chiarle R

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2016

Distinctive Mesenchymal-Parenchymal Cell Pairings Govern B Cell Differentiation in the Bone Marrow.

Stem cell reports

Yu VW,Lymperi S,Oki T,Jones A,Swiatek P,Vasic R,Ferraro F,Scadden DT

15-5790 was used in Flow cytometry/Cell sorting to study the notion that bone marrow is a composite of specialised niches, providing distinctive functional units to regulate hematopoiesis.

Tue Aug 09 00:00:00 EDT 2016

B cell autophagy mediates TLR7-dependent autoimmunity and inflammation.

Autophagy

Weindel CG,Richey LJ,Bolland S,Mehta AJ,Kearney JF,Huber BT

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Mon Apr 18 00:00:00 EDT 2016

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Meningeal Infiltration of the Spinal Cord by Non-Classically Activated B Cells is Associated with Chronic Disease Course in a Spontaneous B Cell-Dependent Model of CNS Autoimmune Disease.

Frontiers in immunology

Dang AK,Tesfagiorgis Y,Jain RW,Craig HC,Kerfoot SM

15-5790 was used in Flow cytometry/Cell sorting to characterise B cell infiltration of the spinal cord in a B cell-dependent spontaneous model of CNS autoimmunity, showing meningeal infiltration of the spinal cord by non-classically activated B cells.

Wed Oct 07 00:00:00 EDT 2015

VprBP Is Required for Efficient Editing and Selection of Igκ+ B Cells, but Is Dispensable for Igλ+ and Marginal Zone B Cell Maturation and Selection.

Journal of immunology (Baltimore, Md. : 1950)

Palmer VL,Aziz-Seible R,Kassmeier MD,Rothermund M,Perry GA,Swanson PC

15-5790 was used in Flow cytometry/Cell sorting to determine the contribution of the protein, VprBP, to B cell development and selection.

Sat Aug 15 00:00:00 EDT 2015

The proximal J kappa germline-transcript promoter facilitates receptor editing through control of ordered recombination.

PloS one

Vettermann C,Timblin GA,Lim V,Lai EC,Schlissel MS

15-5790 was used in Flow cytometry/Cell sorting to identify the proximal GT promoter as the first cis-element known to regulate the internal order of Jκ rearrangements.

Tue Jan 26 00:00:00 EST 2016

Elucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region.

The Journal of experimental medicine

Rouaud P,Saintamand A,Saad F,Carrion C,Lecardeur S,Cogné M,Denizot Y

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Mon May 05 00:00:00 EDT 2014

A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis.

eLife

Olive V,Sabio E,Bennett MJ,De Jong CS,Biton A,McGann JC,Greaney SK,Sodir NM,Zhou AY,Balakrishnan A,Foth M,Luftig MA,Goga A,Speed TP,Xuan Z,Evan GI,Wan Y,Minella AC,He L

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Tue Oct 15 00:00:00 EDT 2013

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Galectin 1 modulates plasma cell homeostasis and regulates the humoral immune response.

Journal of immunology (Baltimore, Md. : 1950)

Anginot A,Espeli M,Chasson L,Mancini SJ,Schiff C

15-5790 was used in Flow cytometry/Cell sorting to elucidate the pattern of GAL1 expression and the function of GAL1 in B cells.

Sat Jun 01 00:00:00 EDT 2013

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

Deletion of genes encoding PU.1 and Spi-B in B cells impairs differentiation and induces pre-B cell acute lymphoblastic leukemia.

Blood

Sokalski KM,Li SK,Welch I,Cadieux-Pitre HA,Gruca MR,DeKoter RP

15-5790 was used in Flow cytometry/Cell sorting to investigate the impairment of differentiation of B cells and induction of pre-B cell acute lymphoblastic leukemia through the deletion of genes encoding PU.1 and Spi-1 in B cells.

Thu Sep 08 00:00:00 EDT 2011

Regulation of follicular B cell differentiation by the related E26 transformation-specific transcription factors PU.1, Spi-B, and Spi-C.

Journal of immunology (Baltimore, Md. : 1950)

DeKoter RP,Geadah M,Khoosal S,Xu LS,Thillainadesan G,Torchia J,Chin SS,Garrett-Sinha LA

15-5790 was used in Flow cytometry/Cell sorting to demonstrate the role of the E26 transformation-specific transcription factors PU.1, Spi-B, and Spi-C in follicular B cell development.

Wed Dec 15 00:00:00 EST 2010

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

miR-19 is a key oncogenic component of mir-17-92.

Genes & development

Olive V,Bennett MJ,Walker JC,Ma C,Jiang I,Cordon-Cardo C,Li QJ,Lowe SW,Hannon GJ,He L

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2009

Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing.

Molecular and cellular biology

Bai L,Chen Y,He Y,Dai X,Lin X,Wen R,Wang D

Published figure using IgM monoclonal antibody (Product # 15-5790-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2007

Bioinformatics

Gene Aliases: AI326478; Igh-6; Igh-M; Igh6; Igm; muH

View more View less

Entrez Gene ID: (Mouse) 16019

View more View less

Function(s)

immunoglobulin

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IgM Monoclonal Antibody (II/41), PE-Cyanine5, eBioscience™

IgM Antibody (15-5790-82) in Flow

Product Details

15-5790-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!