Invitrogen

IgM Monoclonal Antibody (II/41), APC-eFluor™ 780, eBioscience™

31 Published Figures

27 References

View all (30) Mouse IgM antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (31)

FIGURE: 1 / 32

IgM Antibody (47-5790-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or 0.25 µg of Anti-Mouse IgM APC-eFluor® 780 (right). Cells in the small scatter population were used for analysis.

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Product Details

47-5790-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 27 publications 27 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

II/41

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573984

Product Specific Information

Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

Progressive differentiation toward the long-lived plasma cell compartment in the bone marrow.

The Journal of experimental medicine

Koike T,Fujii K,Kometani K,Butler NS,Funakoshi K,Yari S,Kikuta J,Ishii M,Kurosaki T,Ise W

47-5790-82 was used in Flow cytometry/Cell sorting to suggest that the adhesion status of bone marrow plasma cells is dynamically altered during their differentiation and is associated with provision of survival signals.

Mon Feb 06 00:00:00 EST 2023

Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants.

Nature

Xu J,Xu K,Jung S,Conte A,Lieberman J,Muecksch F,Lorenzi JCC,Park S,Schmidt F,Wang Z,Huang Y,Luo Y,Nair MS,Wang P,Schulz JE,Tessarollo L,Bylund T,Chuang GY,Olia AS,Stephens T,Teng IT,Tsybovsky Y,Zhou T,Munster V,Ho DD,Hatziioannou T,Bieniasz PD,Nussenzweig MC,Kwong PD,Casellas R

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

A platform-agnostic, function first-based antibody discovery strategy using plasmid-free mammalian expression of antibodies.

mAbs

Zhang R,Prabakaran P,Yu X,Mackness BC,Boudanova E,Hopke J,Sancho J,Saleh J,Cho H,Zhang N,Simonds-Mannes H,Stimple SD,Hoffmann D,Park A,Chowdhury PS,Rao SP

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Wed Jan 19 00:00:00 EST 2022

Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers.

PloS one

Cohen AA,Yang Z,Gnanapragasam PNP,Ou S,Dam KA,Wang H,Bjorkman PJ

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Fri Oct 15 00:00:00 EDT 2021

Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers.

PloS one

Cohen AA,Yang Z,Gnanapragasam PNP,Ou S,Dam KA,Wang H,Bjorkman PJ

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Fri Oct 15 00:00:00 EDT 2021

Antibody Feedback Limits the Expansion of B Cell Responses to Malaria Vaccination but Drives Diversification of the Humoral Response.

Cell host & microbe

McNamara HA,Idris AH,Sutton HJ,Vistein R,Flynn BJ,Cai Y,Wiehe K,Lyke KE,Chatterjee D,Kc N,Chakravarty S,Lee Sim BK,Hoffman SL,Bonsignori M,Seder RA,Cockburn IA

47-5790 was used in Flow cytometry/Cell sorting to suggest that antibody feedback drives the diversification of immune responses and that vaccination for malaria will require targeting multiple antigens.

Wed Oct 07 00:00:00 EDT 2020

B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches.

Cell reports

Biram A,Winter E,Denton AE,Zaretsky I,Dassa B,Bemark M,Linterman MA,Yaari G,Shulman Z

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Tue Feb 11 00:00:00 EST 2020

Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses.

Cell reports

Fallet B,Hao Y,Florova M,Cornille K,de Los Aires AV,Girelli Zubani G,Ertuna YI,Greiff V,Menzel U,Hammad K,Merkler D,Reddy ST,Weill JC,Reynaud CA,Pinschewer DD

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Tue Jan 28 00:00:00 EST 2020

The cis-Regulatory Atlas of the Mouse Immune System.

Cell

Yoshida H,Lareau CA,Ramirez RN,Rose SA,Maier B,Wroblewska A,Desland F,Chudnovskiy A,Mortha A,Dominguez C,Tellier J,Kim E,Dwyer D,Shinton S,Nabekura T,Qi Y,Yu B,Robinette M,Kim KW,Wagers A,Rhoads A,Nutt SL,Brown BD,Mostafavi S,Buenrostro JD,Benoist C

47-5790-82 was used in Flow Cytometry to generate matched epigenome and transcriptome measurements in 86 primary cell types that span the mouse immune system and its differentiation cascade.

Thu Feb 07 00:00:00 EST 2019

TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells.

The FEBS journal

Schoeler K,Aufschnaiter A,Messner S,Derudder E,Herzog S,Villunger A,Rajewsky K,Labi V

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Fetal liver Mll-AF4+ hematopoietic stem and progenitor cells respond directly to poly(I:C), but not to a single maternal immune activation.

Experimental hematology

Malouf C,Ottersbach K

47-5790-82 was used in Flow Cytometry to assess if the cell-of-origin of t(4;11) MLL-AF4 acute leukemia is sensitive to a viral or bacterial mimic and if maternal immune activation can lead to a full-blown leukemia.

Thu Aug 01 00:00:00 EDT 2019

A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Lineage-Specific Genes Are Prominent DNA Damage Hotspots during Leukemic Transformation of B Cell Precursors.

Cell reports

Boulianne B,Robinson ME,May PC,Castellano L,Blighe K,Thomas J,Reid A,Müschen M,Apperley JF,Stebbing J,Feldhahn N

47-5790 was used in Flow cytometry/Cell sorting to demonstrate lineage-specific transcription predisposes genes in B cells to DNA damage.

Tue Feb 14 00:00:00 EST 2017

Conversion of T cells to B cells by inactivation of polycomb-mediated epigenetic suppression of the B-lineage program.

Genes & development

Ikawa T,Masuda K,Endo TA,Endo M,Isono K,Koseki Y,Nakagawa R,Kometani K,Takano J,Agata Y,Katsura Y,Kurosaki T,Vidal M,Koseki H,Kawamoto H

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2016

Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.

Proceedings of the National Academy of Sciences of the United States of America

Lu G,Duan J,Shu S,Wang X,Gao L,Guo J,Zhang Y

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Tue Feb 02 00:00:00 EST 2016

Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system.

Nature communications

Cheong TC,Compagno M,Chiarle R

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2016

B cell autophagy mediates TLR7-dependent autoimmunity and inflammation.

Autophagy

Weindel CG,Richey LJ,Bolland S,Mehta AJ,Kearney JF,Huber BT

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Mon Apr 18 00:00:00 EDT 2016

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

PD-L1hi B cells are critical regulators of humoral immunity.

Nature communications

Khan AR,Hams E,Floudas A,Sparwasser T,Weaver CT,Fallon PG

47-5790 was used in Flow cytometry/Cell sorting to demonstrate that elevated PD-L1 expression on B cells is an important regulator of T follicular helper-cell activity, downstream antibody responses and autoimmune disease.

Thu Jan 22 00:00:00 EST 2015

Elucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region.

The Journal of experimental medicine

Rouaud P,Saintamand A,Saad F,Carrion C,Lecardeur S,Cogné M,Denizot Y

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Mon May 05 00:00:00 EDT 2014

A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis.

eLife

Olive V,Sabio E,Bennett MJ,De Jong CS,Biton A,McGann JC,Greaney SK,Sodir NM,Zhou AY,Balakrishnan A,Foth M,Luftig MA,Goga A,Speed TP,Xuan Z,Evan GI,Wan Y,Minella AC,He L

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Tue Oct 15 00:00:00 EDT 2013

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

miR-19 is a key oncogenic component of mir-17-92.

Genes & development

Olive V,Bennett MJ,Walker JC,Ma C,Jiang I,Cordon-Cardo C,Li QJ,Lowe SW,Hannon GJ,He L

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2009

Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing.

Molecular and cellular biology

Bai L,Chen Y,He Y,Dai X,Lin X,Wen R,Wang D

Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2007

Bioinformatics

Gene Aliases: AI326478; Igh-6; Igh-M; Igh6; Igm; muH

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Entrez Gene ID: (Mouse) 16019

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Function(s)

immunoglobulin

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IgM Monoclonal Antibody (II/41), APC-eFluor™ 780, eBioscience™

IgM Antibody (47-5790-82) in Flow

Product Details

47-5790-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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