Invitrogen

NK1.1 Monoclonal Antibody (PK136), Super Bright™ 600, eBioscience™

FIGURE: 1 / 50

NK1.1 Antibody (63-5941-82) in Flow

Staining of BALB/c splenocytes (left) or C57BL/6 splenocytes (right) with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971-82) and 0.5 µg of Anti-Mouse NK1.1 Super Bright 600. Cells in the lymphocyte gate were used for analysis.

NK1.1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

63-5941-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 32 publications 32 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 600, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

PK136

Conjugate

Super Bright™ 600 Super Bright™ 600 Super Bright™ 600

Excitation/Emission Max

414/601 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2637451

Product Specific Information

Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Product # 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.

Applications Reported: This PK136 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This PK136 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Peripheral Neutrophils-Derived Matrix Metallopeptidase-9 Induces Postoperative Cognitive Dysfunction in Aged Mice.

Frontiers in aging neuroscience

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Mon Mar 14 00:00:00 EDT 2022

NK cells require immune checkpoint receptor LILRB4/gp49B to control neurotropic Zika virus infections in mice.

JCI insight

Lee HN,Manangeeswaran M,Lewkowicz AP,Engel K,Chowdhury M,Garige M,Eckhaus MA,Sourbier C,Ireland DD,Verthelyi D

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Valosin-containing protein (VCP/p97) inhibition reduces viral clearance and induces toxicity associated with muscular damage.

Cell death & disease

Del Rio Oliva M,Basler M

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The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

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Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

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The Effect of miR-520b on Macrophage Polarization and T Cell Immunity by Targeting PTEN in Breast Cancer.

Journal of oncology

Zhu Q,Yuan J,He Y,Hu Y

Published figure using NK1.1 monoclonal antibody (Product # 63-5941-82) in Immunohistochemistry

Wed Apr 27 00:00:00 EDT 2022

Exosomal lncRNA SNHG10 derived from colorectal cancer cells suppresses natural killer cell cytotoxicity by upregulating INHBC.

Cancer cell international

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Tue Oct 12 00:00:00 EDT 2021

Expression of CCRL2 Inhibits Tumor Growth by Concentrating Chemerin and Inhibiting Neoangiogenesis.

Cancers

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Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.

EBioMedicine

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A novel miR1983-TLR7-IFNβ circuit licenses NK cells to kill glioma cells, and is under the control of galectin-1.

Oncoimmunology

Shah D,Comba A,Faisal SM,Kadiyala P,Baker GJ,Alghamri MS,Doherty R,Zamler D,Nuñez G,Castro MG,Lowenstein PR

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Mon Aug 02 00:00:00 EDT 2021

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

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Sequencing, cloning, and antigen binding analysis of monoclonal antibodies isolated from single mouse B cells.

STAR protocols

Viant C,Escolano A,Chen ST,Nussenzweig MC

Published figure using NK1.1 monoclonal antibody (Product # 63-5941-82) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

The enrichment of neutrophil extracellular traps impair the placentas of systemic lupus erythematosus through accumulating decidual NK cells.

Scientific reports

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Thu Mar 25 00:00:00 EDT 2021

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STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

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Dendritic Cells and Myeloid Derived Suppressor Cells Fully Responsive to Stimulation via Toll-Like Receptor 4 Are Rapidly Induced from Bone-Marrow Cells by Granulocyte-Macrophage Colony-Stimulating Factor.

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Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques.

Nature

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Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

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Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.

JCI insight

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Plasmodium co-infection protects against chikungunya virus-induced pathologies.

Nature communications

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Hypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.

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Oncoimmunology

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Published figure using NK1.1 monoclonal antibody (Product # 63-5941-82) in Flow Cytometry

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Bioinformatics

Protein Aliases: CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; Killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; KLRB1; Ly-55; ly-55b; Ly-55c; Ly-55d; Ly55; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; Lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; MGC163757; MGC163759; natural killer cell receptor protein NKR-P1B; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; Natural killer cell surface protein NKR-P1B allele B6; natural killer cell surface protein NKR-P1B allele SJL/BALB; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; natural killer cells; nk cells; NK1.1; NKR-P1 34; NKR-P1 40; NKR-P1.9; NKR-P1C; NKR-P1D

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Gene Aliases: AI462337; CD161; Klrb1b; Klrb1c; Klrb1d; ly-55c; Ly-59; Ly55b; Ly55c; Ly55d; Ly59; Nk-1; Nk-1.2; NK-RP1; Nk1; NK1.1; Nk1.2; NKR-P1.9; NKR-P1B; NKR-P1D; NKRP1; Nkrp1-b; NKRP140; Nkrp1b; Nkrp1c; Nkrp1d

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UniProt ID: (Mouse) Q99JB4, (Mouse) P27814

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Entrez Gene ID: (Mouse) 80782, (Mouse) 17059

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Function(s)

receptor activity protein binding carbohydrate binding protein homodimerization activity transmembrane signal receptor

Process(es)

negative regulation of natural killer cell mediated cytotoxicity natural killer cell activation positive regulation of natural killer cell mediated cytotoxicity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NK1.1 Monoclonal Antibody (PK136), Super Bright™ 600, eBioscience™

NK1.1 Antibody (63-5941-82) in Flow

Product Details

63-5941-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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