Quant-iT Technology

Invitrogen Quant-iT assay kits use intentionally designed fluorescent dyes which bind selectively to DNA, RNA, protein, or endotoxin. The intensity of the fluorescent signal of the resulting complex is proportional to the amount of the target molecule in the sample.

Key benefits of Quant-iT assays:

• Accuracy—Quant-iT technology gives more accurate results than can be obtained with UV absorbance readings, because the Quant-iT reagents only report the molecule of interest—there’s no interference from free nucleotides or other contaminants.
• Sensitivity—Upon binding to DNA, RNA, or protein, the fluorescence of the Quant-iT dyes increases several hundred fold, giving a very high signal-to-background ratio resulting in accurate low-concentration measurements—up to 1,000 times more sensitive than absorbance-based readings.
  
• Confidence—Confidence comes from knowing your assay is sensitive and accurate enough to quantitate even the most dilute or low-abundance samples, while still leaving enough of your biomolecule for successful downstream applications. With Quant-iT technology, you can get the crucial quantitation data you need, without wasting your sample.
   

Which assay kit is right for me?

Quant-iT assay kits and reagents for use with microplate readers*—for 20–2,000 samples

Quant-iT bulk reagents and kits for >2,000 samples

* Invitrogen Quant-iT assays and reagents can also be used with the Invitrogen Qubit Fluorometer. †The useful range assumes a 1–20 µL sample volume in a 96-well microplate assay. ‡ Excitation (Ex) and emission (Em), in nm, of the Quant-iT detection reagent.