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Primary Antibodies for the Ultra Violet Laser
Detect more with an ultraviolet laser
Ultraviolet (UV) light-excitable fluorophores play a critical role in standard multiparametric flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger multiparameter panels. The Brilliant Ultra Violet™ dyes join the trusted Invitrogen dye portfolio in providing fluorophores compatible with the 355 nm ultraviolet laser. Brilliant Ultra Violet™ dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The expansive Alexa Fluor dye portfolio support options for immunophenotyping using UV excitation. These molecules are conjugated to antibodies used in flow cytometry experiments. By incorporating UV-excitable dyes in experiments, scientists can take advantage of a broader range of instrument lasers and channels. Super Bright, Brilliant Ultra Violet™, and Brilliant Violet™ dyes use similar polymer technologies.
Properties of fluorescent dyes for the ultraviolet laser
The table below lists the properties of the fluorophores available for the ultraviolet laser.
| Fluorophore | Fluorophore type | Ex/Em (nm) | Relative intensity* (1=lowest, 5=highest) | Suggested bandpass filter (nm) | Uses |
|---|---|---|---|---|---|
| Alexa Fluor 350 | Organic dye | 346/442 | 1 | 450/50 | Blue-fluorescent dye with moderate photostability and excitation that matches the 355 nm laser line |
| Brilliant Ultra Violet™ 395 Dye | Polymer dye | 350/395 | 3 | 379/28 | BUV395 has a medium relative brightness and can be used for cell surface, intracellular, and nuclear staining with conventional and spectral flow cytometers |
| Brilliant Ultra Violet™ 496 | Polymer dye | 350/496 | 2 | 515/30, 450 LP | BUV496 has a low-to-medium relative brightness and can be used for cell surface, intracellular, and nuclear staining with conventional and spectral flow cytometers |
| Brilliant Ultra Violet™ 563 | Polymer dye | 350/594 | 4 | Instruments with yellow-green (561nm) laser: 585/15, 535 LP without yellow-green: 560/40, 535 LP | BUV563 has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining with conventional and spectral flow cytometers |
| Brilliant Ultra Violet™ 615 | Polymer dye | 350/616 | 3 | 610/20, 595 LP | BUV615 has a medium relative brightness and can be used for cell surface, intracellular, and nuclear staining with conventional and spectral flow cytometers |
| Brilliant Ultra Violet™ 661 | Polymer dye | 350/660 | 4 | 670/25, 630 LP | BUV661 has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining with conventional and spectral flow cytometers |
| Brilliant Ultra Violet™ 737 | Polymer dye | 348/737 | 4 | 740/35 | This dye has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining; BUV737 may have some cross-laser excitation with the blue and red laser |
| Brilliant Ultra Violet™ 805 | Polymer dye | 348/805 | 3 | 780/60 | This dye has a low-to-medium relative brightness and can be used for cell surface and intracellular staining; nuclear targets may not be expressed at high enough levels to be paired with BUV805 |
| Qdot 525 | Nanocrystal | UV—525 | 3 | 530/30 | Additional choice for multiplexed fluorescence detection |
| Qdot 565 | Nanocrystal | UV—565 | 3 | 574/26 | Additional choice for multiplexed fluorescence detection |
| Qdot 605 | Nanocrystal | UV—561/605 | 3 | 605/20 | Additional choice for multiplexed fluorescence detection |
| Qdot 655 | Nanocrystal | UV—633/655 | 3 | 655/20 | Additional choice for multiplexed fluorescence detection |
| Qdot 705 | Nanocrystal | UV—633/705 | 3 | 695/40 | Additional choice for multiplexed fluorescence detection |
| Qdot 800 | Nanocrystal | UV—633/800 | 3 | 780/60 | Additional choice for multiplexed fluorescence detection |
| * The relative intensity of any fluorophore is dependent upon cell type being studied, reagent used, instrument, and instrument configuration. The relative intensities provided are to be used as a guideline; observed intensities may vary. | |||||
This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.
Contact our technical sales specialists for help choosing antibodies, fluorophores, and reagents for success on the flow cytometer you’ll be using.
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Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.