Brilliant Ultra Violet 661 excitation shown as dotted line and emission shown as solid red histogram
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630 LP		349659(in buffer) 3
flow cytometry

Invitrogen Brilliant Ultra Violet™ 661 dye (BUV661) is a tandem dye excited by the 349 nm UV laser and emits at 659 nm. This dye has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining. BUV661 may have some cross-laser excitation with the red laser.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.

We offer BUV661 dye conjugated to primary antibodies for use in flow cytometry.

Search Brilliant Ultra Violet ™ 661 primary antibodies

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Experimental data using Brilliant Ultra Violet™ 661
Spectral signature of Brilliant Ultra Violet™ 661 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet™ 661 were used for analysis.
Intracellular staining of mouse splenocytes using Brilliant Ultra Violet™ 661. C57BL/6 mouse splenocytes were unstimulated (left) or stimulated for 48 hours with CD3e Monoclonal Antibody, Functional Grade (right). Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody, Brilliant Ultra Violet™ 661. Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation

Cell surface staining of C57BL/6 mouse splenocytes using Brilliant Ultra Violet™ 661. C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, FITC  and 0.25 µg of Rat IgG2a kappa Isotype Control, Brilliant Ultra Violet™ 661 dye  (left) or 0.25 µg of CD4 Monoclonal Antibody, Brilliant Ultra Violet™ 661 dye  (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.

Brilliant Ultra Violet dye background

Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.

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Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.