NovaFluor Blue 690 Dye

NovaFluor Blue 690 dye has a unique emission spectrum when excited by the 488nm laser, which fits in a spectral space that is currently only accessible using cross-laser excited tandem dyes, such as PerCP-Cyanine5.5. Replacing highly cross-laser excited dyes may potentially allow for the inclusion of an additional marker in a complex multicolor panel. NovaFluor Blue 690 dye should generally be paired with retain their fluorescence intensity and spectral signature when stored at 4°C.

• Narrow emission spectrum and minimal cross-laser excitation for better resolution
• Should be paired with highly expressed antigens to minimize spillover
• Can be used to replace cross-laser excited PerCP-Cyanine5.5
• Produces highly stable fluorescence and is compatible with all buffers tested

Use NovaFluor dyes with CellBlox Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Blue 690 dye dashboard

Initial brightness		The NovaFluor Blue 690 dye, excited by the blue laser is optimized for use for cell-surface applications. Can be used for spectral and conventional flow cytometry applications.
Photostability in buffer

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	488	695/40	494	690
	Laser line	Filter	Excitation max	Emission max

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

• Primary antibodies
• NovaFluor Antibody Conjugation Kits
• NovaFluor CD4 Label Characterization Kits
• Custom conjugation services

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 690 dye.
 

Figure 2. Spectral signature of NovaFluor Blue 690 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 690 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
 

Figure 3. C57BL/6 mouse splenocytes were stained with CD3 monoclonal antibody, Super Bright 436 dye (Cat. No. 62-0037-42) only (left) or along with CD19 monoclonal antibody, NovaFluor Blue 690 dye (Cat. No. M004T03B09) (right). Viable cells were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Cat. No. L34962).