SpectraViewer image of Brilliant Violet 711 dye

Brilliant Violet™ dyes, including Brilliant Violet™ 711 (BV711), are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. The violet laser has unique channels far from heavily occupied detectors allowing for larger panels.

BV711 is a tandem dye excited by the 405 nm violet laser and emits at 711 nm. This dye has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining.

When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend that you use Super Bright Complete Staining Buffer (Cat. No. SB-4401-42) or Brilliant Stain Buffer (Cat. No. 00-4409-42) to minimize any non- specific polymer interactions.

BV711 dye dashboard


Initial brightness
 
 
 
 
 
The violet laser-excitable BV711 dye is optimized for use both cell surface and intracellular flow applications. Can be used for spectral flow cytometry applications. 

Photostability in buffer
 
 
 
 
 
 405710/50,
690 LP		407713 
 
Laser line
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Excitation max


Emission max

 

BV711 dye products

We offer BV711 dye conjugated to primary antibodies for use in flow cytometry.

Antibody conjugates

Brilliant Stain Buffer
Super Bright Staining Buffer

Experimental data using BV711 dye products

Figure 1. Excitation and emission of Brilliant Violet™ 711 dye (BV711).

Figure 2. Spectral signature of BV711 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with clone RPA-T8 (hCD8) conjugated to BV711 dye (Cat. No. 407-0088-42) were used for analysis.

Dot plot of Ki-67 BV711 vs CD45R (B220) FITC in unstimulated and stimulated cells

Figure 3. C57BL/6 mouse splenocytes were unstimulated (left) or stimulated for 48 hours with CD3e Monoclonal Antibody, Functional Grade (Cat. No. 16-0031-85) (right). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00) and protocol, with CD45R (B220) Monoclonal Antibody, FITC (Cat. No. 11-0452-82) and 1.0 µg of Ki-67 Monoclonal Antibody, Brilliant Violet™ 711 dye (Cat. No. 407-5698-82). Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (Cat. No. L34962).

Dot plot of samples stained with IgG1 control vs CD3-FITC and CD4-BV711 vs CD3-FITC

Figure 4. Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC (Cat. No. 11-0038-42) and Mouse IgG1 kappa Isotype Control, Brilliant Violet™ 711 dye (Cat. No. 407-4714-81) (left) or CD4 Monoclonal Antibody, Brilliant Violet™ 711dye (Cat. No. 407-0049-42) (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Cat. No. 00-6993-50).

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.

For Research Use Only. Not for use in diagnostic procedures.