Designed for oligonucleotide synthesis for diagnostic research and development

Dye-labeled phosphoramidites are intended for oligonucleotide synthesis designed for molecular assays, microscopy, and microarrays. A broad range of labeling options are available with coupling efficiency, emission, and absorption spectra comparable to other commercially available dyes.

These phosphoramidites may be used on automated DNA synthesizers following standard synthesis and deprotection procedures. Fluorescent tags can be incorporated at the 5′ end of an oligonucleotide, and DyLight dye–labeled phosphoramidites can be used in research applications without the constraints of licensing and royalty fees.

Our dye-labeled phosphoramidites are supported by:

  • Technical expertise—over 40 years of partnership, customization, and scale-up experience
  • Secure and consistent supply—5x capacity expansion in 2022 to support emerging therapeutics and diagnostic assays
  • Service excellence—48 hours or less response to orders, >98% delivery to customer at promised date†
  • Flexible packaging—custom and bulk packaging available upon request
  • Refined quality—ISO 9001:2015–certified quality management system that has been refined in partnership with our customers since 1995

 

Quality manufacturing and documentation

Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility. Purity of each phosphoramidite lot may be assessed by HPLC, NMR, and water determination by the Karl Fischer method. Our rigorously controlled manufacturing processes help minimize levels of reactive impurities which could be incorporated into the oligonucleotide during synthesis and result in the accumulation of by-products.

 

DyLight-labeled phosphoramidites

Thermo Scientific DyLight (DY) fluorescent labels offer choices with spectrally distinct colors for oligonucleotide labeling with ß-cyanoethyl phosphoramidites (CEP).

Chemical structures and technical specifications

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 DY547 CEPDY647 CEPDY677 CEP
Cat. No.SY6332SY6334SY6336
FormulaC38H54IN4O2PC47H63N4O5PSC55H67N4O5PS
Molecular weight756.74 g/mol827.07 g/mol927.18 g/mol
HPLC purity≥90% at 547 nm≥85% at 647 nm≥85% at 677 nm
IdentityUV-Vis spectrum conforms to that of the authentic sample
Shipping / storage conditionDry ice / –20°C
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Additional dye-labeled phosphoramidites

Phosphoramidites with fluoresceine and its derivatives like 6-carboxyfluorescein (6-FAM), hexachlorofluorescein (HEX), tetrachlorofluorescein (TET) are also available to provide you with flexibility in oligo labeling.

Chemical structures and technical specifications

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 Fluorescein CEP6-FAM CEPHEX CEPTET CEP
Cat. No.27-160627-1608SY6342SY6341
Chemical name[(3′,6′-dipivaloylfluoresceinyl)-5,6-carboamidohexyl]-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Fluorescein dipivalate phosphoramidite, 6-isomer

6-(4,7,2′,4′,5′,7′-hexachloro-3′,6′-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite5′-tetrachloro-fluorescein phosphoramidite
FormulaC46H58N3O10P C46H58N3O10PC46H52N3O10Cl6PC46H54N3O10Cl4P
Molecular weight843.95 g/mol843.94 g/mol1050.63 g/mol981.73 g/mol
CAS / PubChem#

N/A

204697-37-0 / 58114954

1360547-55-2877049-90-6
HPLC purity≥90%≥90%≥95%≥95%
31P NMR purity85%≥95% (6-isomer)N/AN/A
Shipping / storage  conditionDry ice / –20°C
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For flexible/bulk packaging, custom services, quotes, or items not listed above, please contact us or  submit your inquiry.

 

Resources

For Research Use Only. Not for use in diagnostic procedures.