Designed for oligonucleotide synthesis for diagnostic and therapeutic development

Thermo Scientific RNA phosphoramidites are built to meet the varying needs of oligonucleotide manufacturers for the development of therapeutic, diagnostic, and research applications. We offer TheraPure, standard, and fast deprotect phosphoramidites. 

  • TheraPure phosphoramidites undergo additional quality control release testing and are compared with our standard phosphoramidites, helping to ensure that impurities and residual solvents are controlled to the stringent levels required by our customers for their oligo therapeutics.
  • Standard phosphoramidites support the quality and reliability suitable for routine oligonucleotide synthesis.
  • Fast deprotect phosphoramidites allow the removal of base protecting groups under mild or accelerated conditions without the need for special reagents. 

Our RNA phosphoramidites are supported by:

  • Expert technical support—over 40 years of partnership, customization, and scale-up experience 
  • Secure and consistent supply—5x capacity expansion in 2022 
  • Service excellence—48 hours or less response to orders on standard business days, >98% delivery to customer at promised date†
  • Flexible packaging—custom and bulk packaging available upon request
  • Quality—ISO 9001:2015–certified quality management system that has been refined in partnership with our customers since 1995
†May not be applicable in all regions.

Quality manufacturing and regulatory documentation

Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility. Purity of each phosphoramidite lot is assessed by HPLC-MS, GC, 31P NMR, as well as Karl Fischer oven titration. Our rigorously controlled manufacturing processes help minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide during synthesis and result in the accumulation of by-products.

Available RNA phosphoramidites by synthesizer

  Applied Biosystems/AKTA SynthesizerExpedite SynthesizerMerMade SynthesizerBulk
TheraPure phosphoramiditesBz-rA  ✓✓✓✓
Ac-rC✓✓✓✓
iBu-rG  ✓✓✓✓
rU✓✓✓✓
Standard phosphoramiditesBz-rA  ✓   
Ac-rC✓   
iBu-rG  ✓   
rU✓   
Fast deprotection (mild)PAC-rA✓✓  
iPrPAC-rG✓✓  
Ac-rGContact us

For items not listed above, flexible/bulk packaging, or custom services, please contact us ›

Product information

TheraPure RNA Phosphoramidites

TheraPure RNA phosphoramidites were engineered specifically for oligonucleotide manufacturers requiring highly defined impurity profiles, strictly controlled processes with thorough documentation, and a rigorous process change control.  With our in-house advanced analytical capabilities including LC-MS, GC, HPLC, 31P NMR and 1H NMR, meeting possible regulatory requirements should be achievable. Collaborative, customized specifications are always welcome for further discussion. 

  • QC testing always performed on raw materials and intermediates using high-definition analytics 
  • State-of-the-art purification methods utilized to obtain the highest amidite purity
  • Overall, high purity and tight critical impurity level control yields excellent batch-to-batch reproducibility

Order now

  Click images to enlarge

 Bz-rA CEPiBu-rG CEPAc-rC CEPrU CEP
Cat. No. 27-190327-190627-180527-1804
FormulaC53H66N7O8PSiC50H68N7O9PSiC47H64N5O9PSiC45H61N4O9PSi
Molecular weight

988.2 g/mol970.1 g/mol902.1 g/mol861.1 g/mol
CAS #104992-55-4147201-04-5121058-88-6118362-03-1
AppearanceColor intensity must not exceed Pantone #P1-4U or #P4-2U
Solution Clarity  Free from undissolved particulate at 0.2M solution in ACN
HPLC purity

≥99%

31P NMR purity

≥99%

Residual Solvent≤3.0% total % by weight solvent residue
Shipping / Storage conditionAmbient / –20°C
Water content ≤0.3% by KF Method
Material OriginNon-animal source (no TSE/BSE risk)
 Request a quote
RNA PAC Phosphoramidites – Fast Deprotection (mild)

Use these additional amidites with Ac-rC and U to mitigate the time for your oligonucleotide deprotection.

Order now

  Click images to enlarge

 Therapure PAC-rA CEPTherapure iPrPAC-rG CEP
Cat. No.27-180327-1806
FormulaC54H68N7O9PSiC57H74N7O10PSi
Molecular weight1018.2 g/mol1076.3 g/mol
CAS #121058-86-4956580-73-7
Solution Clarity0.2M solution in ACN is free from undissolved particulate
HPLC purity≥99%
31P NMR purity

≥99%

Water content ≤0.3% (KF Method)
Shipping / Storage  conditionAmbient / –20°C
Material Origin Non-animal source (no TSE/BSE risk)
 Request a quote
Standard RNA Phosphoramidites

Traditional base protection methods are used to ensure quality and reliability for routine oligonucleotide synthesis.

Order now

  Click images to enlarge

 Bz-rA CEPiBu-rG CEPAc-rC CEPrU CEP
Cat. No.27-140327-140627-140527-1404
FormulaC53H66N7O8PSiC50H68N7O9PSiC47H64N5O9PSiC45H61N4O9PSi
Molecular weight

988.2 g/mol970.1 g/mol902.1 g/mol861.1 g/mol
CAS #104992-55-4147201-04-5121058-88-6118362-03-1
AppearanceColor intensity must not exceed Pantone #P1-4U or #P4-2U
HPLC purity≥98.0%
31P NMR purity 

≥98.0%

Shipping / Storage conditionAmbient / –20°C
Water content ≤0.3% by KF Method
Material OriginNon-animal source (no TSE/BSE risk)
 Request a quote

RNA phosphoramidite ordering information

TheraPure RNA Phosphoramidites ordering information
Fast Deprotection (mild), Therapure phosphoramidites ordering information
Standard RNA Phosphoramidites ordering information
Resources

For Research Use Only. Not for use in diagnostic procedures.