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Ion exchange chromatography (IEX) is a purification technique that allows for the separation of molecules based on their surface charge. The process exploits the interaction between negatively or positively charged molecules in a sample and an oppositely charged chromatography matrix.
Ion exchange chromatography serves as the workhorse in downstream processing and plays a significant role in impurity removal. Our large portfolio of anion and cation exchange resins are applied in many commercial and late-stage processes for the manufacturing of:
A major improvement to the performance of traditional ion exchangers, POROS resins provide an easy solution for ion exchange chromatography in bind/elute or flow through modes of operation.
POROS IEX resin | Bind/Elute application | Flow through application |
---|---|---|
Cation exchange | Polish of many biomolecules such as:
| Polish for mAbs by binding impurities under normal B/E conditions such as:
|
Anion exchange
| Capture of:
Polish of:
| Trace impurity removal by binding impurities such as:
|
Choosing the right ion exchange resin will help to ensure quality, productivity, and effectiveness of the purification process. Biomolecules vary in their net charge depending on the solution pH. Select either an anion exchange or cation exchange resin according the purification step and charged properties of the target molecule.
The point along the pH scale at which the molecule or surface carries a zero-net charge.
POROS resins exhibit high capacity, high resolution, and salt tolerance, built to deliver exceptional separation capability and to meet the increasing demands of process intensification. High performance resins, like POROS, can simplify the development process, balance all the purification requirements, and help develop more efficient and cost-effective downstream processes.
Selecting the optimal resin for your processesDownload the article and learn how efficient purification strategies are critical to overcoming manufacturing limitations. |
Process development scientists are looking for new tools that will clear even more impurities in a single step while maintaining high recoveries and process flexibility. Watch this video to learn how Thermo Scientific POROS Ion Exchange chromatography resins address purification challenges of today’s biomolecules.
Use this interactive decision-making tool to determine the right chromatography resin for your purification process.
Check out the new Chromatography Learning Lab from Thermo Fisher Scientific, providing a robust range of webinars, articles, eBooks, infographics, and other digital resources to help you optimize the use of our purification products in your process.
Explore digital resource
Access a targeted collection of scientific application notes, case studies, posters, white papers and more for bioprocessing:
Pharmaceutical Grade Reagent. For Manufacturing and Laboratory Use Only.