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The increasing number of approved nucleic acid therapeutics drives the demand for efficient platform technologies to manufacture these new therapeutic modalities. We have built a toolbox of high-performing chromatography resins to help address your needs and help you increase efficiency and productivity in the purification of mRNA and plasmid DNA.
One challenge we can help you overcome is obtaining larger quantities of synthetic mRNA to support clinical manufacturing. POROS™ Oligo (dT)25 Affinity Resin has been designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes. This affinity chromatography resin offers high selectivity and allows for easy mRNA purification. Through the AT-base pairing mechanism, the resin effectively separates mRNA from components of the IVT process, such as enzymes, unreacted nucleotides, partial transcripts, and plasmid DNA.
Figure 1. Mechanism of action of the POROS Oligo (dT)25 affinity resin. The Poly-dT ligand allows binding with poly-A tailed mRNA molecules through AT base pairing.
Figure 2. Chromatogram showing efficient separation of a 2000nt mRNA from an IVT mixture at a load concentration of 2 mg/mL. Elution was performed using H2O and yielded in >95% recovery.
The rapid growth of the gene therapy pipeline as well as genetic vaccination for various infectious diseases, requires larger scale production of plasmid DNA (pDNA) at high quality. POROS AEX resins are ideally suited for the purification of larger molecules such as plasmids and will help you overcome the common challenges of plasmid purification.
Plasmid purification challenge | POROS resin features |
Products and contaminants are similar in size | 50-micron bead size—providing superior resolution, tighter peaks and improved impurity clearance |
Shear sensitivity and high viscosity limits operational flow rates | Poly(styrene-divinylbenzene) backbone — resulting in linear and scalable performance. The beads are rigid and have a high mechanical strength enabling easy and reproducible scale-up. |
Conventional chromatography resins exhibit low binding capacities for pDNA | Large throughpores — leading to a reduced mass transfer resistance compared to other resins. Capacity and resolution are maintained over a wide range of linear velocities, thereby establishing a more efficient purification process. |
Plasmids are generally much larger than proteins | Large throughpores — increased surface area accessible which favours the purification of larger molecules |
During a design of experiments (DoE) study, POROS AEX resins where tested using various process conditions to determine dynamic binding capacities for plasmid DNA binding. Download the article below to learn more about the complete study.
POROS Resin | Plasmid DBC10% (mg/mL resin) |
POROS HQ | 2.8 |
POROS D50 | 15.6 |
POROS XQ | 9.0 |
Table 1. Dynamic Binding Capacity Determination using process conditions from DoE study. Process conditions: residence time 2.5 min, 1 mL column, 45 mS/cm conductivity
A design of experiment (DoE) study was conducted in order to evaluate POROS AEX resins for pDNA capture, with the goals of optimizing process conditions to help maximize purity and recovery, determine the dynamic binding capacity (DBC) of POROS AEX resins for pDNA,and confirm optimal operating parameters.
For subsequent polishing steps after mRNA or plasmid DNA capture, we recommend our suite of differentiated novel Hydrophobic Interaction Chromatography resins.
Use this interactive decision-making tool to determine the right chromatography resin for your purification process.
Check out the new Chromatography Learning Lab from Thermo Fisher Scientific, providing a robust range of webinars, articles, eBooks, infographics, and other digital resources to help you optimize the use of our purification products in your process.
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