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DNA Phosphoramidites |
Thermo Scientific phosphoramidites with deoxyribonucleobases for synthesis of DNA oligos are built to meet the varying requirements of oligonucleotide manufacturers from the proof of concept of research and development (R&D) to the process development of therapeutic and diagnostic applications. Backed by documentation and certificates of analysis, choose from multiple quality grades of TheraPure, standard, and fast deprotect phosphoramidites that best fit your needs.
Premium-grade and high-purity phosphoramidites are manufactured under extreme quality control and QC testing before product release to achieve critical impurity levels. Excellent for any therapeutics application with excellent batch-to-batch reproducibility.
Extensive QC testing to help ensure impurities and residual solvents are within the stringent requirement for oligo therapeutics.
Bz-dA | Bz-dC | iBu-dG | dT | |
---|---|---|---|---|
Applied Biosystems / AKTA Synthesizer | 27-2030-12 | 27-2032-12 | 27-2034-12 | 27-2036-12 |
Expedite Synthesizer | 27-2030-84 | 27-2032-84 | 27-2034-84 | 27-2036-84 |
MerMade Synthesizer | 27-2030-14 | 27-2032-14 | 27-2034-14 | 27-2036-14 |
Click images to enlarge
dA(Bz) CEP | dC(Bz) CEP | dG(iBu) CEP | dT CEP | |
---|---|---|---|---|
Cat. No. | 27-2030 | 27-2032 | 27-2034 | 27-2036 |
Formula | C47H52N7O7P | C46H52N5O8P | C44H54N7O8P | C40H49N4O8P |
Molecular weight | 857.95 g/mol | 833.93 g/mol | 839.92 g/mol | 744.82 g/mol |
CAS # | 98796-53-3 | 102212-98-6 | 93183-15-4 | 98796-51-1 |
HPLC purity | ≥99% | |||
31P NMR purity | ≥99% | |||
Solution clarity | Free from undissolved particulate at 0.2 M solution in ACN | |||
Appearance | White to pale yellow powder | |||
Residual solvent | ≤3.0% total % by weight | |||
Shipping/Storage condition | Ambient / –20°C | |||
Water content | ≤0.3% | |||
Material origin | Non-animal source (no TSE/BSE risk) |
Compatible products are in amber bottles with serum vial neck style as ready-to-ship catalog SKU. Available in sizes of 1 g or 2 g.
Available TheraPure DNA phosphoramidites are compatible with the following oligonucleotide synthesizers:
Available catalog SKUs are supplied in 30 mL amber bottle with a 20-400 finish neck style, which is compatible with the Expedite synthesizer for oligo synthesis.
Catalog SKUs available that are compatible with the MerMade synthesizer for oligo synthesis.
Traditional base protection methods to help ensure quality and reliability of routine oligonucleotide synthesis. This quality grade supports research application (research use only, RUO) with a smooth transition to our TheraPure grade amidites, which are designed with quality attributes for therapeutics.
Bz-dA | Bz-dC | iBu-dG | dT | dU | dI | |
---|---|---|---|---|---|---|
Applied Biosystems/AKTA Synthesizer | 27-1730-04 | 27-1732-04 | 27-1734-04 | 27-1736-04 | 27-1738-02 | 27-1744-03 |
Expedite Synthesizer | 27-1730-11 | 27-1732-11 | 27-1734-11 | 27-1736-11 | 27-1738-08 | |
MerMade Synthesizer | 27-1730-13 | 27-1732-13 | 27-1734-13 | 27-1736-13 |
Click images to enlarge
dA(Bz) CEP | dC(Bz) CEP | dG(iBu) CEP | dT CEP | |
---|---|---|---|---|
Cat. No. | 27-1730 | 27-1732 | 27-1734 | 27-1736 |
Formula | C47H52N7O7P | C46H52N5O8P | C44H54N7O8P | C40H49N4O8P |
Molecular weight | 857.95 g/mol | 833.93 g/mol | 839.92 g/mol | 744.82 g/mol |
CAS # | 98796-53-3 | 102212-98-6 | 93183-15-4 | 98796-51-1 |
HPLC purity | ≥98.0% | |||
31P NMR purity | ≥98.0% | |||
Water content | ≤0.3% | |||
Shipping/Storage condition | Ambient / –20°C | |||
Solution clarity | Free from undissolved particulate at 0.2 M solution in ACN | |||
Material origin | Non-animal source (no TSE/BSE risk) |
Standard DNA phosphoramidites are compatible with the following oligonucleotide synthesizers:
All compatible products are available as ready-to-ship catalog SKU in sizes of 1 g or 5 g. For additional size options or custom packaging services, please contact us.
Available catalog SKUs compatible with the Expedite synthesizer for oligo synthesis.
Catalog SKUs available that are compatible with the MerMade synthesizer for oligo synthesis.
Fast deprotect phosphoramidites permit the removal of base protecting groups under mild or accelerated conditions without the need for special reagents. Excellent for applications requiring mild deprotection to preserve the integrity of oligonucleotides containing sensitive dyes, labels, or other modifications.
PAC-dA | Ac-dC | DMF-dG | iPrPAC-dG | |
---|---|---|---|---|
Applied Biosystems/AKTA Synthesizer | 27-1723-04 | 27-2027-07 | 27-1737-07 | 27-1726-04 |
Expedite Synthesizer | 29-1727-10 | 27-1737-10 | ||
MerMade Synthesizer | 29-1727-13 | 27-1737-13 |
Click images to enlarge
iPrPAC-dG CEP | PAC-dA CEP | Ac-dC CEP | DMF-dG CEP | IBU-dC CEP | |
---|---|---|---|---|---|
Cat. No. (TheraPure phosphoramidites) | N/A | N/A | 27-2027 | N/A | N/A |
Cat. No. (standard phosphoramidites) | 27-1726 | 27-1723 | 29-1727 | 27-1737 | 27-1725 |
Formula | C51H60N7O9P | C48H54N7O8P | C41H50N5O8P | C43H53N8O7P | C43H54N5O8P |
Molecular weight | 946.05 g/mol | 887.96 g/mol | 771.8 g/mol | 824.9 g/mol | 839.92g/mol |
CAS # | 150065-82-0 | 110543-74-3 | 154110-40-4 | 330628-04-1 | 110522-84-4 |
HPLC purity | (TheraPure phosphoramidites) | ≥98.0% | ≥98.0% | ≥98% | (≥99%) | ≥98.0% | ≥98.0% |
31P NMR purity | (TheraPure phosphoramidites) | ≥98.0% | ≥98.0% | ≥98% | (≥99%) | ≥98.0% | ≥98.0% |
Shipping/Storage condition | Ambient / –20°C | ||||
Material origin | Non-animal source (no TSE/BSE risk) |
Standard DNA phosphoramidites are compatible with the following oligonucleotide synthesizers:
All compatible products are available as ready-to-ship catalog SKU in size ranges of 100 μM, 100 µmol, 0.25 g, 1 g or 5 g. For additional size options or custom packaging services, please contact us.
Available catalog SKUs compatible with the Expedite synthesizer for oligo synthesis.
Catalog SKUs available that are compatible with the MerMade synthesizer for oligo synthesis.
HPLC-MS, GC, 31P NMR, and Karl Fischer oven titration are used to assess the purity of each phosphoramidite lot. The rigorously controlled manufacturing processes help to minimize levels of reactive P(III) impurities and accumulation of by-products during oligo synthesis. Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility under the ISO 9001:2015–certified quality management system.
Phosphoramidites are the key raw material and building blocks of oligonucleotide therapeutics. Incorporating the quality testing step into the manufacturing process development from the very beginning is a best practice needed for regulatory documentation.
For the successful synthesis of a therapeutic from discovery to market, a thorough understanding on the analysis of phosphoramidites and impurity is crucial. Here is information on the analytical method used and the details on the instrument conditions used to test the quality of the raw material phosphoramidites.
Condition setup | |
---|---|
UHPLC system | Thermo Scientific Vanquish UHPLC System |
Detection | Thermo Scientific Vanquish Diode Array Detector, 260 nm |
Column | Phenomenex Luna C18(2) Column, 2.0 x 150 mm, 3 µm (Cat. No. 00F-4251-B0) |
Column temperature | 25°C |
Sample temperature | 4°C |
Injection volume | 2.0 µL |
Mobile phase A | 5 mM tributylammonium formate, 30% acetonitrile |
Mobile phase B | 5 mM tributylammonium formate, 100% acetonitrile |
Sample preparation | 5 mg/mL in acetonitrile dried over molecular sieves |
Gradient | |||
---|---|---|---|
Time (min) | Flow (mL/min) | A (%) | B (%) |
0 | 0.25 | 78 | 22 |
45 | 0.25 | 10 | 90 |
69 | 0.25 | 10 | 90 |
70 | 0.25 | 78 | 22 |
90 | 0.25 | 78 | 22 |
Speak with a technical specialist about support available for the oligonucleotide manufacturing process development of your unique projects.
Access webinars, videos, articles, and more to optimize your success in oligonucleotide synthesis for diagnostic and therapeutic manufacturing process development.
Browse the educational assets related to raw materials selection, process development using in vitro transcription, scale-up synthesis, and the latest trends in mRNA production.
For Research Use Only. Not for use in diagnostic procedures.