CELLstart xeno-free substrate

CELLstart Substrate—the first xeno-free substrate for attachment and expansion of human embryonic, mesenchymal, and neural stem cells.

  • Fully-defined
  • Better lot-to-lot consistency
  • Produced under cGMP

First xeno-free stem cell culture substrate

Containing components only of human origin, CELLstart xeno-free substrate maintains:

  • Pluripotency and differentiation capability of human embryonic stem cells (hESCs) in STEMPRO® hESC SFM (serum- and feeder-free medium)
  • Multipotency and tri-lineage mesoderm differentiation potential of human mesenchymal stem cells (hMSCs) in STEMPRO MSC SFM (serum-free medium)
  • Multipotency of human neural stem cells (hNSCs) in serum-free medium
  • Consistent performance lot to lot and produced under cGMP
  • Little or no adaptation from feeders or feeder-free substrates of animal origin
     

Attachment and growth of hESCs

hESC lines (BG01V, H9 and RCM1) grown on CELLstart xeno-free substrate in serum- and feeder-free hESC medium exhibit normal morphology (Figure 1), retain pluripotency, and maintain pluripotent differentiation capabilities to all three germ layers.

hESCs grown on CELLstart-coated dishes in STEMPRO hESC SFM exhibit normal morphology

Figure 1. hESCs grown on CELLstart-coated dishes in STEMPRO hESC SFM exhibit normal morphology. (A) Phase contrast image (4X) of BG01V cells (passage 13). (B) Phase contrast image (10X) of H9 cells (passage 60). Data provided by E. Seftor, Children’s Memorial Research Center, Chicago, IL. (C) Phase contrast image (10X) of RCM1 cells (passage 28). Data provided by B. Tye, Roslin Cells Ltd.

Attachment and growth of hMSCs

hMSCs grown on CELLstart in STEMPRO MSC SFM exhibit superior growth compared to expansion in classical medium (DMEM + 10% MSC-Qualified FBS) and maintain tri-lineage mesoderm differentiation potential beyond passage 5 (Figure 2).

Figure 2.  hMSCs grown on CELLstart-coated dishes in STEMPRO MSC SFM retain tri-lineage differentiation potential through long-term passaging. hMSCs (passage 5) were seeded into adipogenic, chondrogenic, or osteogenic differentiation medium for 14 days, revealing adipocytes (oil red O lipid stain), chondrocytes (alcian blue glycosaminoglycan stain) and osteoblasts (alkaline phosphatase cell surface glycoprotein stain).

Attachment and growth of hNSCs

hNSCs grown on CELLstart xeno-free substrate in serum-free medium exhibit normal morphology while maintaining multipotency of these cells (Figure 3).

hNSCs grown on CELLstart-coated dishes in serum-free medium exhibit normal morphology and express hNSC markers

Figure 3. hNSCs grown on CELLstart-coated dishes in serum-free medium exhibit normal morphology and express hNSC markers. (A) Phase contrast image (10x) of hNSCs (passage 27). (B) Immunofluorescence analysis of hNSC (passage 23) shows nestin expression and C. Sox2 expression.

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