StemPro® Human Adipose-Derived Stem Cell Kit | Thermo Fisher Scientific

Low passage
Each lot from single donor
MesenPRO RS Medium reduces ADSC doubling time

Human adipose-derived stem cells

Human ADSCs isolated from human lipoaspirate tissue and cryopreserved from primary cultures
Low passage—ADSCs passaged once after isolation
Expanded in MesenPRO RS Medium—a revolutionary new reduced-serum (2%) medium that reduces ADSC doubling times
Kit contains human ADSCs and MesenPRO RS Medium—components ensure optimal ADSC growth and expansion

ADSCs have demonstrated very similar phenotypic and functional characteristics to bone marrow-derived mesenchymal stem cells. They can be expanded to 4–5 passages before they lose their ability to grow or differentiate into all potential phenotypes.

ADSC Characterization

Figure 1. The cell surface protein profile of ADSCs was analyzed by flow cytometry at P2 or P3 and the following criteria was used to characterize the ADSCs

Positive markers:
CD29, CD44, CD73, CD90, CD105, CD166 (>95%)

Negative markers:
CD14, CD31, CD45, Lin1 (< 2%).

Bar graph showing percentage of ADSCs expressing various cell surface proteins.

Differentiation potential of Human ADSCs

Each vial of ADSCs contains cells that can differentiate into multiple mature cell phenotypes in vitro including adipocytes, osteoblasts, and chondrocytes (Figure 1). In vitro differentiation into non-mesenchymal cell types, such as neuronal and glial progenitors, hepatocytes and vascular endothelial progenitors, cardiomyocytic, pancreatic and epithelial phenotypes has also been described. In addition, ADSCs secrete pro-angiogenic, immunomodulatory, and anti-apoptotic factors. ADSCs can be used for studies of adult stem cell differentiation, tissue engineering, and potential future clinical applications or as cells amenable to delivery of recombinant DNA constructs.

Figure 1: Three panel image showing ADSC-derived chondrocytes stained with safranin orange, ADSC-derived osteoblasts stained with Alizarin red, and ADSC-derived adipocytes stained with Oil Red O.
Figure 1. Differentiation potential of human ADSCs

A. ADSCs induced to differentiate towards chondrocytes for 29 days and then stained with Safranin Orange dye ( pellet cross-sectional staining) for proteoglycan content, image captured using 4x objective lens.

B. ADSCs induced to differentiate towards osteoblasts for 29 days and then stained with Alizarin Red dye (which stains mineralized extracellular matrix), image captured using 4x objective lens.

C. ADSCs induced to differentiate towards adipocytes for 14 days and then stained with Oil-Red-O (which stains lipid vacuoles) and counterstained with hematoxylin, image captured using 10x objective lens.